FastQCFastQC Report
Sat 18 Jun 2016
SRR3553498_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553498_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047742
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT103730.9900338060324011No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCC80390.7672690414243201No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGT73290.6995042672719047No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT69150.6599907229069752Illumina Single End Adapter 2 (95% over 22bp)
AACTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG24610.23488606928041447No Hit
AATGACTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTT21360.203866982520506No Hit
AATGATCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCT20140.19222289456755576No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA13270.12665331732430313No Hit
AATGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTC12870.12283558356923746No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC12610.12035405662844478Illumina Single End Adapter 2 (95% over 21bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCATAGATC10590.10107450116536322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG351.2117926E-745.0000041
AATTGCG700.045.0000041
ATAGTCG351.2117926E-745.0000041
CGCTTAG453.8562575E-1045.0000041
CGGCGAA253.8905688E-545.030
CATATCG207.033002E-445.019
TTTCGCG253.8905688E-545.01
TCATCCG253.8905688E-545.01
CGACTAG406.8139343E-945.01
TAGTGCG253.8905688E-545.01
CGGTAAG207.033002E-445.01
CCTGCGA207.033002E-445.01
TAGTCCG253.8905688E-545.01
TGCGTAA253.8905688E-545.029
AGATACG551.8189894E-1244.9999961
ATATCCG551.8189894E-1244.9999961
CGTAACG950.042.631581
AAATCCG603.6379788E-1241.2500041
TACCGCG603.6379788E-1241.2500041
ACGATTG556.184564E-1140.909091