SRR3553496_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553496_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	179861
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	3954	2.198364292425818	Illumina Single End Adapter 1 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCC	2255	1.2537459482600453	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGT	2032	1.129761315682666	No Hit
AATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	1915	1.0647110824470007	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1309	0.7277842333802214	No Hit
AAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	1216	0.6760776377313592	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT	1033	0.5743324011319852	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	573	0.31857934738492505	No Hit
AAGCGGGGGAGAGCTCTGGAGCCGCAGAGAGGACAGGCAGAATTTGAAACC	566	0.3146874530887741	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	565	0.31413146818932397	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT	505	0.2807723742223161	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	417	0.23184570307070462	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	400	0.22239395978005239	No Hit
AACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	400	0.22239395978005239	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTAT	376	0.20905032219324923	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	352	0.1957066846064461	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTGAGGTTC	324	0.18013910742184241	No Hit
AACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	298	0.16568350003613902	No Hit
ACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	288	0.16012365104163773	No Hit
TACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	267	0.14844796815318495	No Hit
GAAAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT	267	0.14844796815318495	No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	264	0.14678001345483455	No Hit
TCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	252	0.14010819466143298	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	249	0.1384402399630826	No Hit
AGCCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	243	0.13510433056638183	No Hit
ATTCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAAGTGCTTGCCTAGCA	237	0.13176842116968102	No Hit
TCGACAGGGATATCTGTTTCCCTTTCCTCCTGTGCTAGTAGAACAAACAAG	234	0.13010046647133064	No Hit
CAAAAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	226	0.1256525872757296	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	216	0.12009273828122828	No Hit
TTCTGAGGGAACTTAATTTGTAGAAAACAATGGATTCAGTTAATATCATTT	212	0.11786879868342776	No Hit
TAAAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT	191	0.10619311579497502	No Hit
GAAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTC	189	0.10508114599607475	No Hit
ATTTCAGGGATTATAGGATTTGTATTTTCTTCTCCTCCCCTCCTCTTCCTC	187	0.1039691761971745	No Hit
TAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	186	0.10341319129772435	No Hit
TAAGATGGGATTGATACCACTGCAGAAATAAGCCAGCAACAACAGGGCAGC	185	0.10285720639827423	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	30	2.1552642E-6	45.000004	3
TCGTTTG	30	2.1552642E-6	45.000004	21
ACTGCGG	30	2.1552642E-6	45.000004	2
CTCGTTT	30	2.1552642E-6	45.000004	20
CCCTCCA	30	2.1552642E-6	45.000004	34
ACGTTAC	30	2.1552642E-6	45.000004	10
CAGTTTA	30	2.1552642E-6	45.000004	42
ACACCGG	30	2.1552642E-6	45.000004	2
TTGCTCA	30	2.1552642E-6	45.000004	10
CTATATT	30	2.1552642E-6	45.000004	40
GGCAATT	30	2.1552642E-6	45.000004	8
CACGCTT	30	2.1552642E-6	45.000004	1
GATCAAC	30	2.1552642E-6	45.000004	9
AATATGG	30	2.1552642E-6	45.000004	2
CGTTACT	30	2.1552642E-6	45.000004	11
GCATTAG	30	2.1552642E-6	45.000004	1
GGGACGC	30	2.1552642E-6	45.000004	7
GGAACTT	30	2.1552642E-6	45.000004	8
AAACCGG	65	0.0	45.000004	2
AGTTAAG	30	2.1552642E-6	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content