##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553496_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	179861
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.423532616854125	31.0	31.0	34.0	30.0	34.0
2	31.63605784466894	31.0	31.0	34.0	30.0	34.0
3	31.81531849594965	31.0	31.0	34.0	30.0	34.0
4	35.49304184898338	37.0	35.0	37.0	33.0	37.0
5	35.24398841327469	37.0	35.0	37.0	33.0	37.0
6	35.229315971778206	37.0	35.0	37.0	32.0	37.0
7	35.06500019459472	37.0	35.0	37.0	32.0	37.0
8	35.46455318273556	37.0	35.0	37.0	33.0	37.0
9	37.04330010396917	39.0	37.0	39.0	33.0	39.0
10	36.680208605534276	38.0	35.0	39.0	32.0	39.0
11	36.75989792117246	39.0	35.0	39.0	32.0	39.0
12	36.67579408543264	39.0	35.0	39.0	32.0	39.0
13	36.59272994145479	39.0	35.0	39.0	32.0	39.0
14	37.63159328592636	39.0	36.0	41.0	32.0	41.0
15	37.694363981074275	39.0	37.0	41.0	33.0	41.0
16	37.56373532894847	39.0	36.0	41.0	32.0	41.0
17	37.58031479865007	39.0	36.0	41.0	32.0	41.0
18	37.48469095579364	39.0	36.0	40.0	32.0	41.0
19	37.365476673653546	39.0	36.0	40.0	32.0	41.0
20	37.38588687931236	39.0	36.0	40.0	32.0	41.0
21	37.30550814239885	39.0	36.0	40.0	32.0	41.0
22	37.333290707824375	39.0	36.0	40.0	32.0	41.0
23	37.2047525589205	39.0	36.0	40.0	32.0	41.0
24	37.039113537676315	39.0	35.0	40.0	31.0	41.0
25	37.099643613679454	39.0	35.0	40.0	31.0	41.0
26	37.03199137111436	39.0	35.0	40.0	31.0	41.0
27	37.020499163242725	39.0	35.0	40.0	31.0	41.0
28	36.96384430198876	39.0	35.0	40.0	31.0	41.0
29	36.69570946453094	39.0	35.0	40.0	31.0	41.0
30	36.6594536892378	39.0	35.0	40.0	30.0	41.0
31	36.594848243921696	39.0	35.0	40.0	30.0	41.0
32	36.27386704177115	38.0	35.0	40.0	30.0	41.0
33	36.31251911198092	38.0	35.0	40.0	30.0	41.0
34	36.2328575955877	38.0	35.0	40.0	30.0	41.0
35	35.966529709053106	38.0	35.0	40.0	29.0	41.0
36	36.040025352911414	38.0	35.0	40.0	30.0	41.0
37	35.71805449763984	38.0	35.0	40.0	28.0	41.0
38	35.71079333485302	38.0	35.0	40.0	28.0	41.0
39	35.56880591123145	38.0	34.0	40.0	27.0	41.0
40	35.393359316360964	38.0	34.0	40.0	26.0	41.0
41	35.357514969893415	38.0	34.0	40.0	26.0	41.0
42	35.14686341118975	38.0	34.0	40.0	26.0	41.0
43	35.176069297957866	38.0	34.0	40.0	26.0	41.0
44	35.13128471430716	38.0	34.0	40.0	26.0	41.0
45	34.87580409316083	38.0	33.0	40.0	25.0	41.0
46	34.59174584818276	38.0	33.0	40.0	24.0	41.0
47	34.59307465209245	38.0	33.0	40.0	24.0	41.0
48	34.50254919076398	37.0	33.0	40.0	24.0	41.0
49	34.20699317806528	37.0	33.0	40.0	23.0	41.0
50	34.09157627278843	37.0	32.0	40.0	23.0	41.0
51	32.59405874536448	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	1.0
14	7.0
15	16.0
16	23.0
17	45.0
18	98.0
19	215.0
20	398.0
21	571.0
22	804.0
23	1008.0
24	1231.0
25	1450.0
26	1666.0
27	2070.0
28	2602.0
29	3312.0
30	4264.0
31	5514.0
32	7076.0
33	9222.0
34	12393.0
35	15641.0
36	18603.0
37	24886.0
38	34785.0
39	31947.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.591145384491355	37.23264076147692	20.623147875303708	25.553065978728018
2	17.272226886317767	40.02535291141493	21.09073117574127	21.61168902652604
3	18.98632833132252	33.223433651542024	24.781914923190687	23.008323093944767
4	21.74790532689132	31.76786518478158	19.938174479181146	26.546055009145952
5	16.05184003202473	38.55032497317373	18.830096574577034	26.567738420224508
6	20.358499063165443	36.52431599957745	22.655272682793935	20.46191225446317
7	70.59340268318313	10.778323260740239	10.248469651564264	8.379804404512374
8	73.0108250259923	6.66236705011092	7.971155503416528	12.35565242048026
9	65.7941410311296	11.528346890098465	15.926743429648452	6.75076864912349
10	25.022100399753143	40.26609437287683	15.477507630892745	19.23429759647728
11	18.994668104814274	25.888324873096447	35.624732432267145	19.492274589822138
12	20.98175813544904	22.31723386392826	38.60091959902369	18.100088401599013
13	23.355257671201652	25.722641373060306	30.363447328770548	20.55865362696749
14	14.894835456269007	28.361345705850628	36.517088195884604	20.226730641995765
15	12.929428836712795	31.147386036995233	32.60629041315238	23.31689471313959
16	16.238650958239976	29.61453566921122	29.533361873891508	24.613451498657295
17	15.447484446322438	29.595076197730467	32.21487704393949	22.742562312007607
18	15.879484713195188	26.874086099821533	29.838041598790177	27.408387588193108
19	16.054619956521982	33.72215210634879	33.3340746465326	16.889153290596628
20	17.91772535457937	31.770089124379382	28.984604778134226	21.327580742907024
21	16.30036528207894	31.740621924708524	28.10837257660082	23.85064021661172
22	14.87092810559265	29.58896036383652	28.625438533089444	26.914672997481386
23	19.9620818298575	27.708619433896175	24.61956733255125	27.709731403695077
24	14.709136499852665	33.78219847548941	25.74766069353556	25.76100433112237
25	19.298235859914044	27.545159873457838	26.935244438761043	26.221359827867076
26	20.25008200777267	29.72239673970455	25.262285876315598	24.76523537620718
27	15.296812538571453	27.287182880112976	29.325979506396603	28.090025074918966
28	25.99396200399197	25.465776349514346	26.913561027682487	21.626700618811192
29	20.404645809819804	30.05821161897243	23.797265666264504	25.73987690494326
30	26.15519762483251	25.072695025603103	24.58454028388589	24.187567065678497
31	28.889531360328252	27.507908885194677	18.440907144961944	25.16165260951513
32	22.552415476395662	28.121160229288172	24.255397223411414	25.071027070904755
33	29.38491390573832	23.544292537014694	24.61956733255125	22.451226224695738
34	25.38682649379243	24.984849411489986	22.859875125791586	26.768448968926005
35	26.450425606440533	22.204924914239328	28.692712705922908	22.651936773397235
36	27.521808507680934	21.90246912893846	30.87717737586247	19.698544987518137
37	24.180895246885097	27.60075836340285	25.625344015656538	22.59300237405552
38	29.34265905338011	24.636802864434202	28.22512940548535	17.79540867670034
39	27.64523715535886	23.43643146652137	26.101267089585846	22.817064288533924
40	29.64733877827878	23.9890804565748	24.256509193210313	22.107071571936107
41	24.32878723013883	19.475595042838638	32.39557213626078	23.800045590761755
42	27.079244527718625	22.782593224768018	28.090581059818415	22.04758118769494
43	20.47025202795492	20.41965740210496	31.99303906905888	27.117051500881235
44	24.437760270431056	19.861448563057028	28.28573175952541	27.415059406986508
45	28.44363147096925	22.81595231873502	27.294410683805825	21.446005526489902
46	21.155225424077482	22.408971372337525	34.385998076292246	22.049805127292743
47	24.977065622897683	22.49792895624955	26.14963777583801	26.375367645014762
48	23.121744013432597	19.130884405179554	35.78207615881153	21.96529542257632
49	23.747783010213443	17.978327708619435	32.16317044829062	26.1107188328765
50	21.65060796948755	18.337493953664218	27.43563084826616	32.576267228582076
51	21.079055492852813	16.469384691511777	36.36975219753032	26.081807618105092
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1536.0
1	1298.0
2	1060.0
3	731.0
4	402.0
5	356.0
6	310.0
7	310.5
8	311.0
9	343.5
10	376.0
11	454.5
12	533.0
13	512.5
14	492.0
15	504.5
16	517.0
17	632.0
18	747.0
19	754.5
20	762.0
21	865.5
22	969.0
23	1015.5
24	1062.0
25	953.5
26	1110.0
27	1375.0
28	1380.0
29	1385.0
30	2009.5
31	2634.0
32	3030.0
33	3426.0
34	3658.0
35	3890.0
36	4087.5
37	4285.0
38	4545.5
39	4806.0
40	5598.5
41	6391.0
42	6570.5
43	6750.0
44	8922.5
45	11095.0
46	15934.0
47	20773.0
48	21454.5
49	22136.0
50	23677.5
51	25219.0
52	21678.0
53	18137.0
54	14463.0
55	10789.0
56	9068.5
57	7348.0
58	6559.0
59	5770.0
60	4850.0
61	3930.0
62	3673.5
63	3417.0
64	2839.0
65	2261.0
66	1877.0
67	1493.0
68	1267.5
69	1042.0
70	843.5
71	645.0
72	554.5
73	464.0
74	331.0
75	163.5
76	129.0
77	90.5
78	52.0
79	42.5
80	33.0
81	29.0
82	25.0
83	16.5
84	8.0
85	8.0
86	8.0
87	13.0
88	18.0
89	10.0
90	2.0
91	2.0
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	179861.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.802647600091184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.20312136035406	27.282735000917373
2	10.93407873282087	7.829379354056744
3	4.195977948598493	4.506813594942762
4	2.0048140383570154	2.871106020760476
5	1.2004037580557496	2.1488816363747563
6	0.7469524031368895	1.604572419813078
7	0.573025856044724	1.4361089952796882
8	0.34164143178818235	0.9785334230322305
9	0.29039521701995497	0.9357225857745702
>10	2.880658436213992	23.544848521914144
>50	0.532650050469757	12.458509626878534
>100	0.07919869555089681	5.549841266311207
>500	0.006211662396148769	1.2281706428853392
>1k	0.010870409193260346	7.624776911059096
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	3954	2.198364292425818	Illumina Single End Adapter 1 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCC	2255	1.2537459482600453	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGT	2032	1.129761315682666	No Hit
AATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	1915	1.0647110824470007	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1309	0.7277842333802214	No Hit
AAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	1216	0.6760776377313592	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT	1033	0.5743324011319852	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	573	0.31857934738492505	No Hit
AAGCGGGGGAGAGCTCTGGAGCCGCAGAGAGGACAGGCAGAATTTGAAACC	566	0.3146874530887741	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	565	0.31413146818932397	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT	505	0.2807723742223161	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	417	0.23184570307070462	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	400	0.22239395978005239	No Hit
AACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	400	0.22239395978005239	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTAT	376	0.20905032219324923	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	352	0.1957066846064461	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTGAGGTTC	324	0.18013910742184241	No Hit
AACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	298	0.16568350003613902	No Hit
ACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	288	0.16012365104163773	No Hit
TACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	267	0.14844796815318495	No Hit
GAAAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT	267	0.14844796815318495	No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	264	0.14678001345483455	No Hit
TCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	252	0.14010819466143298	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	249	0.1384402399630826	No Hit
AGCCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	243	0.13510433056638183	No Hit
ATTCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAAGTGCTTGCCTAGCA	237	0.13176842116968102	No Hit
TCGACAGGGATATCTGTTTCCCTTTCCTCCTGTGCTAGTAGAACAAACAAG	234	0.13010046647133064	No Hit
CAAAAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	226	0.1256525872757296	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	216	0.12009273828122828	No Hit
TTCTGAGGGAACTTAATTTGTAGAAAACAATGGATTCAGTTAATATCATTT	212	0.11786879868342776	No Hit
TAAAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT	191	0.10619311579497502	No Hit
GAAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTC	189	0.10508114599607475	No Hit
ATTTCAGGGATTATAGGATTTGTATTTTCTTCTCCTCCCCTCCTCTTCCTC	187	0.1039691761971745	No Hit
TAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	186	0.10341319129772435	No Hit
TAAGATGGGATTGATACCACTGCAGAAATAAGCCAGCAACAACAGGGCAGC	185	0.10285720639827423	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.5202795492074435	0.0
2	0.0	0.0	0.0	3.3097781064266294	0.0
3	0.0	0.0	0.0	4.191014172055087	0.0
4	0.0	0.0	0.0	8.772329743524166	0.0
5	0.0	0.0	0.0	9.3449941899578	0.0
6	0.0	0.0	0.0	12.760965412179406	0.0
7	0.0	0.0	0.0	15.307376251661005	0.0
8	0.0	0.0	0.0	17.19994884938925	0.0
9	0.0	0.0	0.0	20.783827511244795	0.0
10	0.0	0.0	0.0	24.614563468456197	0.0
11	0.0	0.0	0.0	28.777222410639325	0.0
12	0.0	0.0	0.0	30.39013460394416	0.0
13	0.0	0.0	0.0	31.063988302077714	0.0
14	0.0	0.0	0.0	31.901301560649614	0.0
15	0.0	0.0	0.0	32.4745219919827	0.0
16	0.0	0.0	0.0	33.67489338989553	0.0
17	0.0	0.0	0.0	35.15325723753343	0.0
18	0.0	0.0	0.0	37.62627807028761	0.0
19	0.0	0.0	0.0	38.43190018959085	0.0
20	0.0	0.0	0.0	39.350387243482466	0.0
21	0.0	0.0	0.0	40.10652670673465	0.0
22	0.0	0.0	0.0	40.924936478725236	0.0
23	0.0	0.0	0.0	41.67996397217851	0.0
24	0.0	0.0	0.0	42.17979439678418	0.0
25	0.0	0.0	0.0	42.60901473915968	0.0
26	0.0	0.0	0.0	43.06992622080384	0.0
27	0.0	0.0	0.0	43.57364853970566	0.0
28	0.0	0.0	0.0	44.056243432428374	0.0
29	0.0	0.0	0.0	44.54495415904504	0.0
30	0.0	0.0	0.0	45.22825960046925	0.0
31	0.0	0.0	0.0	45.6580359277442	0.0
32	0.0	0.0	0.0	46.14563468456197	0.0
33	0.0	0.0	0.0	46.567071238345164	0.0
34	0.0	0.0	0.0	47.01575105220142	0.0
35	0.0	0.0	0.0	47.45609109256593	0.0
36	0.0	0.0	0.0	47.84806044667827	0.0
37	0.0	0.0	0.0	48.24002980079061	0.0
38	0.0	0.0	0.0	48.62866324550625	0.0
39	0.0	0.0	0.0	49.065111391574604	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	30	2.1552642E-6	45.000004	3
TCGTTTG	30	2.1552642E-6	45.000004	21
ACTGCGG	30	2.1552642E-6	45.000004	2
CTCGTTT	30	2.1552642E-6	45.000004	20
CCCTCCA	30	2.1552642E-6	45.000004	34
ACGTTAC	30	2.1552642E-6	45.000004	10
CAGTTTA	30	2.1552642E-6	45.000004	42
ACACCGG	30	2.1552642E-6	45.000004	2
TTGCTCA	30	2.1552642E-6	45.000004	10
CTATATT	30	2.1552642E-6	45.000004	40
GGCAATT	30	2.1552642E-6	45.000004	8
CACGCTT	30	2.1552642E-6	45.000004	1
GATCAAC	30	2.1552642E-6	45.000004	9
AATATGG	30	2.1552642E-6	45.000004	2
CGTTACT	30	2.1552642E-6	45.000004	11
GCATTAG	30	2.1552642E-6	45.000004	1
GGGACGC	30	2.1552642E-6	45.000004	7
GGAACTT	30	2.1552642E-6	45.000004	8
AAACCGG	65	0.0	45.000004	2
AGTTAAG	30	2.1552642E-6	45.000004	1
>>END_MODULE