FastQCFastQC Report
Sat 18 Jun 2016
SRR3553492_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553492_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382019
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT57110.41323599747905054RNA PCR Primer, Index 41 (96% over 27bp)
AATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT25490.18444030074839782Illumina PCR Primer Index 11 (95% over 23bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA24100.17438255190413446No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCC15980.11562793275635139No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGT15290.11063523728689693No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG253.891217E-545.01
ATTACGC253.891217E-545.012
TAACGGC207.033784E-445.013
ATTCACG800.045.01
AATTGCG551.8189894E-1245.01
CGCGAAT302.1658216E-644.99999629
TACCGGA302.1658216E-644.9999963
CGCACCG1250.043.1999971
CGAAACG750.042.01
ACGATAG700.041.7857131
CGTAACG1350.041.6666641
TCTAGCG650.041.538461
CGAATAT3000.041.2513
CCGATAG603.6379788E-1241.2499961
ATTACCG556.184564E-1140.9090921
CGGGTTA1050.040.7142876
ATAACGG2100.040.7142872
CTACGAA3100.040.6451610
GCTACGA3100.040.645169
TCTTGCG900.040.0000041
Produced by FastQC (version 0.11.5)