##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553492_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1382019
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.963733494257315	33.0	31.0	34.0	30.0	34.0
2	32.16551653776106	34.0	31.0	34.0	30.0	34.0
3	32.30299149288107	34.0	31.0	34.0	30.0	34.0
4	35.90738332830446	37.0	35.0	37.0	35.0	37.0
5	35.71890256212107	37.0	35.0	37.0	33.0	37.0
6	35.728390130671144	37.0	35.0	37.0	33.0	37.0
7	35.85673713603069	37.0	35.0	37.0	35.0	37.0
8	36.02298665937299	37.0	35.0	37.0	35.0	37.0
9	37.646842047757666	39.0	37.0	39.0	35.0	39.0
10	37.16158316202599	39.0	37.0	39.0	34.0	39.0
11	37.139065381879696	39.0	37.0	39.0	34.0	39.0
12	37.14631781473337	39.0	37.0	39.0	34.0	39.0
13	37.126917213149746	39.0	37.0	39.0	33.0	39.0
14	38.361373468816275	40.0	38.0	41.0	34.0	41.0
15	38.349965521458095	40.0	38.0	41.0	34.0	41.0
16	38.28299610931543	40.0	38.0	41.0	33.0	41.0
17	38.30813107489839	40.0	38.0	41.0	34.0	41.0
18	38.286671890907435	40.0	38.0	41.0	34.0	41.0
19	38.2965053302451	40.0	38.0	41.0	34.0	41.0
20	38.250176010604775	40.0	38.0	41.0	34.0	41.0
21	38.19980767268757	40.0	37.0	41.0	34.0	41.0
22	38.198890898026725	40.0	37.0	41.0	34.0	41.0
23	38.098382873173236	40.0	37.0	41.0	33.0	41.0
24	38.01910176343451	40.0	37.0	41.0	33.0	41.0
25	38.020670482822595	40.0	37.0	41.0	33.0	41.0
26	37.9565143460401	40.0	37.0	41.0	33.0	41.0
27	37.91997794531045	40.0	37.0	41.0	33.0	41.0
28	37.86656261599877	40.0	37.0	41.0	33.0	41.0
29	37.7753699478806	40.0	37.0	41.0	33.0	41.0
30	37.7527993464634	40.0	37.0	41.0	33.0	41.0
31	37.68058688049875	40.0	37.0	41.0	33.0	41.0
32	37.66583310359698	40.0	37.0	41.0	33.0	41.0
33	37.57131703688589	40.0	37.0	41.0	32.0	41.0
34	37.57855210384228	40.0	37.0	41.0	33.0	41.0
35	37.532398613911965	40.0	37.0	41.0	32.0	41.0
36	37.44504742698906	40.0	36.0	41.0	32.0	41.0
37	37.3846336410715	40.0	36.0	41.0	32.0	41.0
38	37.36706803596767	39.0	36.0	41.0	32.0	41.0
39	37.293943136816495	39.0	36.0	41.0	32.0	41.0
40	37.19320211950777	39.0	36.0	41.0	31.0	41.0
41	37.16236462740382	39.0	36.0	41.0	31.0	41.0
42	37.131917144409734	39.0	36.0	41.0	31.0	41.0
43	37.06451647915115	39.0	35.0	41.0	31.0	41.0
44	36.920144368492764	39.0	35.0	41.0	31.0	41.0
45	36.731139731074606	39.0	35.0	40.0	31.0	41.0
46	36.604564047238135	39.0	35.0	40.0	30.0	41.0
47	36.49273562809194	39.0	35.0	40.0	30.0	41.0
48	36.47361505160204	39.0	35.0	40.0	30.0	41.0
49	36.316788698274046	39.0	35.0	40.0	30.0	41.0
50	36.16156941402397	38.0	35.0	40.0	29.0	41.0
51	34.91228702355033	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	6.0
10	6.0
11	7.0
12	3.0
13	13.0
14	23.0
15	41.0
16	81.0
17	133.0
18	243.0
19	438.0
20	810.0
21	1280.0
22	1969.0
23	2761.0
24	3725.0
25	4782.0
26	6554.0
27	8672.0
28	11861.0
29	15724.0
30	20924.0
31	27650.0
32	36869.0
33	49697.0
34	71939.0
35	93280.0
36	121040.0
37	180835.0
38	304076.0
39	416426.0
40	147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.858179229084406	32.190440218260385	19.777296838900188	27.17408371375502
2	23.739109230770346	30.33916320976774	20.382643075095206	25.539084484366715
3	20.50688159858873	30.77113990473358	20.699281268926114	28.02269722775157
4	20.361152777204943	31.438931013249455	19.83612381595333	28.363792393592274
5	18.628687449304245	33.54150702703798	18.427098324986847	29.40270719867093
6	21.449994536978146	35.317242382340616	19.884820686256845	23.34794239442439
7	87.19909060584551	3.7273004206165035	4.77178678440745	4.30182218913054
8	88.91643313152714	3.276365954447804	3.6081269504977866	4.199073963527274
9	80.86437306578274	7.66878024108207	7.188540823244832	4.278305869890356
10	30.556381641641682	42.87155241715201	12.802284194356229	13.769781746850079
11	22.742957947756146	27.529939892288024	32.048039860522906	17.679062299432932
12	26.452313607844758	24.097208504369334	30.98741768383792	18.463060203947993
13	24.79459399617516	25.761802117047594	28.32688986186152	21.11671402491572
14	19.862462093502334	28.160973184883854	29.307990700562	22.66857402105181
15	17.91089702818847	30.44314151976203	30.131857810927347	21.514103641122155
16	20.369908083752826	28.760313714934455	30.0663739065816	20.803404294731116
17	19.400095078287634	28.410029095113742	30.48264893608554	21.70722689051308
18	21.237841158479007	27.37545576435635	28.1937513160094	23.192951761155236
19	21.039508139902562	30.895161354511046	27.181247146385108	20.884083359201284
20	25.026718156552118	27.951714122598894	26.81743159826312	20.20413612258587
21	22.67074475821244	29.65834767828807	26.205066645248724	21.465840918250763
22	21.706286237743473	26.98891983395308	27.20215858103253	24.102635347270912
23	21.084080609600882	30.427295138489413	26.54254391582171	21.946080336088
24	19.853923860670513	28.021394785455193	28.980281747211865	23.14439960666243
25	21.024312979778138	29.253143408303362	25.90456426431185	23.817979347606656
26	23.37847743048395	29.440188593644518	25.604278957091037	21.577055018780495
27	21.310271421738776	28.51046186774567	26.39717688396469	23.782089826550866
28	21.087119641625765	29.436643056282147	28.079787615076203	21.39644968701588
29	22.131244215889943	28.624787358205637	25.51585759674795	23.728110829156474
30	23.543236380975948	26.9425384166209	27.23023344831004	22.283991754093105
31	21.551512678190388	29.25082795533202	25.033736873371492	24.1639224931061
32	22.728848156212035	27.610980746284962	25.826924231866567	23.833246865636433
33	21.502454018360094	29.226298625416874	25.453195650711024	23.81805170551201
34	19.914704501168217	27.106429072248645	28.87138309965348	24.107483326929657
35	21.03227234936712	26.83132431609117	26.77495750782008	25.361445826721628
36	20.120635099806876	30.582719919190694	26.727780153529	22.56886482747343
37	20.977425057108476	29.956896395780376	25.126716781751917	23.93896176535923
38	20.870624788805365	27.984058106292313	27.750052640376143	23.395264464526175
39	20.410211437035237	26.907444832524007	26.937907510678215	25.744436219762534
40	22.33934555168923	26.90223506333849	28.80995123800758	21.948468146964693
41	18.69815103844448	26.520330038877905	30.983510356948784	23.798008565728836
42	22.813506905476697	27.306788112175013	26.812945408131146	23.06675957421714
43	21.82784751873889	27.626320622220103	27.12227545352126	23.42355640551975
44	23.446349145706392	27.043767126211726	25.914911444777534	23.594972283304354
45	23.04772944510893	26.44030219555592	26.754480220604783	23.757488138730366
46	19.47230826783134	26.821555998868323	28.185357798988292	25.520777934312044
47	20.373453621115196	28.58122789918228	27.738547733424795	23.306770746277728
48	22.015399209417527	25.319188809994653	29.14576427675741	23.519647703830408
49	20.901304540675635	26.589721270112783	27.346150812687814	25.162823376523768
50	19.18960593161165	27.070322477476793	27.461416955917393	26.278654634994165
51	19.356535619264278	24.876937292468483	29.479189504630543	26.287337583636695
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	923.0
1	799.0
2	675.0
3	537.5
4	400.0
5	458.5
6	517.0
7	575.0
8	633.0
9	721.0
10	809.0
11	973.0
12	1137.0
13	1315.5
14	1494.0
15	1775.5
16	2057.0
17	2175.0
18	2293.0
19	2520.0
20	2747.0
21	3659.0
22	4571.0
23	5732.5
24	6894.0
25	7504.0
26	10788.0
27	13462.0
28	16956.0
29	20450.0
30	24278.5
31	28107.0
32	34992.0
33	41877.0
34	44553.5
35	47230.0
36	53657.5
37	60085.0
38	62164.5
39	64244.0
40	66802.5
41	69361.0
42	73954.5
43	78548.0
44	88302.5
45	98057.0
46	109598.0
47	121139.0
48	129136.0
49	137133.0
50	130305.0
51	123477.0
52	114318.5
53	105160.0
54	92446.5
55	79733.0
56	70610.5
57	61488.0
58	57612.0
59	53736.0
60	50905.5
61	48075.0
62	42602.0
63	37129.0
64	30244.0
65	23359.0
66	18420.5
67	13482.0
68	11017.0
69	8552.0
70	6846.5
71	5141.0
72	3969.5
73	2798.0
74	2893.0
75	2477.5
76	1967.0
77	1525.5
78	1084.0
79	764.5
80	445.0
81	344.5
82	244.0
83	176.0
84	108.0
85	81.0
86	54.0
87	39.0
88	24.0
89	14.0
90	4.0
91	8.0
92	12.0
93	6.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1382019.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.01351218299309
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.99294063371897	19.71795691081459
2	10.643451277544724	5.750340015100955
3	3.8265580092355442	3.101063142042425
4	1.9374183955855222	2.093459017308177
5	1.2007267443662377	1.6217923268691494
6	0.8682233925449081	1.407225791524288
7	0.708895583616338	1.3404831639142958
8	0.5326567378976163	1.1511143422840489
9	0.4704016057631223	1.1436479557363455
>10	5.545618991773479	34.42288773801886
>50	1.1160241930019226	20.618081654961053
>100	0.15143585083392172	5.793261386055066
>500	0.0037657227451640875	0.6750206717800822
>1k	0.0016138811764988949	0.7486989237913849
>5k	2.689801960831491E-4	0.4149669597993594
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	5711	0.41323599747905054	RNA PCR Primer, Index 41 (96% over 27bp)
AATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	2549	0.18444030074839782	Illumina PCR Primer Index 11 (95% over 23bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	2410	0.17438255190413446	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCC	1598	0.11562793275635139	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGT	1529	0.11063523728689693	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4701816689929733	0.0
2	0.0	0.0	0.0	0.681611468438567	0.0
3	0.0	0.0	0.0	0.9398568326484658	0.0
4	0.0	0.0	0.0	2.0739946411735293	0.0
5	0.0	0.0	0.0	2.296278126422285	0.0
6	0.0	0.0	0.0	3.542281256625271	0.0
7	0.0	0.0	0.0	4.492991775076899	0.0
8	0.0	0.0	0.0	5.381546852829086	0.0
9	0.0	0.0	0.0	6.547160350183319	0.0
10	0.0	0.0	0.0	8.241131272435473	0.0
11	0.0	0.0	0.0	9.697912980935863	0.0
12	0.0	0.0	0.0	10.385964302950972	0.0
13	0.0	0.0	0.0	10.717146435758119	0.0
14	0.0	0.0	0.0	11.067865203010957	0.0
15	0.0	0.0	0.0	11.36192773037129	0.0
16	0.0	0.0	0.0	11.88174692243739	0.0
17	0.0	0.0	0.0	12.571679549991716	0.0
18	0.0	0.0	0.0	13.333029430130845	0.0
19	0.0	0.0	0.0	13.750245112404388	0.0
20	0.0	0.0	0.0	14.181715302032751	0.0
21	0.0	0.0	0.0	14.689812513431436	0.0
22	0.0	0.0	0.0	15.274681462411154	0.0
23	0.0	0.0	0.0	15.867003275642375	0.0
24	0.0	0.0	0.0	16.27387177745024	0.0
25	0.0	0.0	0.0	16.644416610770186	0.0
26	0.0	0.0	0.0	16.998463841669327	0.0
27	0.0	0.0	0.0	17.374652591606917	0.0
28	0.0	0.0	0.0	17.753952731474747	0.0
29	0.0	0.0	0.0	18.171096055842938	0.0
30	0.0	0.0	0.0	18.75292597279777	0.0
31	0.0	0.0	0.0	19.182804288508336	0.0
32	0.0	0.0	0.0	19.641263976833894	0.0
33	0.0	0.0	0.0	20.06180812275374	0.0
34	0.0	0.0	0.0	20.44848876896772	0.0
35	0.0	0.0	0.0	20.881984979946008	0.0
36	0.0	0.0	0.0	21.29493154580364	0.0
37	0.0	0.0	0.0	21.70520086916316	0.0
38	0.0	0.0	0.0	22.091013220512888	0.0
39	0.0	0.0	0.0	22.49759229069933	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	25	3.891217E-5	45.0	1
ATTACGC	25	3.891217E-5	45.0	12
TAACGGC	20	7.033784E-4	45.0	13
ATTCACG	80	0.0	45.0	1
AATTGCG	55	1.8189894E-12	45.0	1
CGCGAAT	30	2.1658216E-6	44.999996	29
TACCGGA	30	2.1658216E-6	44.999996	3
CGCACCG	125	0.0	43.199997	1
CGAAACG	75	0.0	42.0	1
ACGATAG	70	0.0	41.785713	1
CGTAACG	135	0.0	41.666664	1
TCTAGCG	65	0.0	41.53846	1
CGAATAT	300	0.0	41.25	13
CCGATAG	60	3.6379788E-12	41.249996	1
ATTACCG	55	6.184564E-11	40.909092	1
CGGGTTA	105	0.0	40.714287	6
ATAACGG	210	0.0	40.714287	2
CTACGAA	310	0.0	40.64516	10
GCTACGA	310	0.0	40.64516	9
TCTTGCG	90	0.0	40.000004	1
>>END_MODULE