SRR3553491_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553491_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1397769
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	5733	0.4101536090727438	RNA PCR Primer, Index 41 (96% over 27bp)
AATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	2493	0.17835565104105186	Illumina PCR Primer Index 11 (95% over 23bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	2450	0.17527932011655717	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCC	1714	0.12262398150195061	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGT	1607	0.11496892548053363	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCATCCG	45	3.8562575E-10	45.000004	1
CGGCGTA	25	3.8912407E-5	45.0	35
TCGCACC	20	7.0338114E-4	45.0	41
CGAACCG	20	7.0338114E-4	45.0	1
CGTATAG	20	7.0338114E-4	45.0	1
TATCGAT	20	7.0338114E-4	45.0	13
CCGATAG	30	2.1658398E-6	44.999996	1
CGATAAG	30	2.1658398E-6	44.999996	1
ACGATAG	100	0.0	42.75	1
CGCAATG	100	0.0	42.75	1
CGCACCG	95	0.0	42.63158	1
CTTAACG	85	0.0	42.35294	1
ATTCACG	80	0.0	42.1875	1
CGAATAT	390	0.0	41.538464	13
ATATGCG	60	3.6379788E-12	41.249996	1
AATTGCG	60	3.6379788E-12	41.249996	1
CTAGACG	55	6.184564E-11	40.90909	1
CGTATAT	55	6.184564E-11	40.90909	45
CAAGCCG	55	6.184564E-11	40.90909	1
CACGTAT	55	6.184564E-11	40.90909	43