##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553491_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1397769
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93875597469968	33.0	31.0	34.0	30.0	34.0
2	32.13619990141432	34.0	31.0	34.0	30.0	34.0
3	32.2731252445862	34.0	31.0	34.0	30.0	34.0
4	35.85819616832252	37.0	35.0	37.0	35.0	37.0
5	35.69069281118697	37.0	35.0	37.0	33.0	37.0
6	35.71020604978362	37.0	35.0	37.0	33.0	37.0
7	35.8609977757412	37.0	35.0	37.0	35.0	37.0
8	36.02616669850311	37.0	35.0	37.0	35.0	37.0
9	37.64718204510187	39.0	37.0	39.0	35.0	39.0
10	37.15175397365373	39.0	37.0	39.0	34.0	39.0
11	37.14329477903716	39.0	37.0	39.0	34.0	39.0
12	37.15023440926219	39.0	37.0	39.0	34.0	39.0
13	37.119745823523054	39.0	37.0	39.0	33.0	39.0
14	38.35094854729215	40.0	38.0	41.0	34.0	41.0
15	38.336531286643215	40.0	38.0	41.0	34.0	41.0
16	38.29730162852374	40.0	38.0	41.0	33.0	41.0
17	38.29432188008176	40.0	38.0	41.0	34.0	41.0
18	38.26249187097439	40.0	38.0	41.0	33.0	41.0
19	38.272540026284744	40.0	38.0	41.0	34.0	41.0
20	38.215632912162164	40.0	38.0	41.0	34.0	41.0
21	38.18050192843023	40.0	37.0	41.0	34.0	41.0
22	38.17112198081371	40.0	37.0	41.0	34.0	41.0
23	38.07822894913251	40.0	37.0	41.0	33.0	41.0
24	38.012718124382495	40.0	37.0	41.0	33.0	41.0
25	38.01737483089123	40.0	37.0	41.0	33.0	41.0
26	37.935468593165254	40.0	37.0	41.0	33.0	41.0
27	37.88966345655112	40.0	37.0	41.0	33.0	41.0
28	37.85086520018687	40.0	37.0	41.0	33.0	41.0
29	37.74783243869337	40.0	37.0	41.0	33.0	41.0
30	37.74386611807817	40.0	37.0	41.0	33.0	41.0
31	37.669167079825066	40.0	37.0	41.0	33.0	41.0
32	37.65426261420878	40.0	37.0	41.0	33.0	41.0
33	37.56430497457019	40.0	37.0	41.0	32.0	41.0
34	37.55954381589519	40.0	37.0	41.0	32.0	41.0
35	37.50107635811067	40.0	37.0	41.0	32.0	41.0
36	37.44578610628795	40.0	36.0	41.0	32.0	41.0
37	37.36420109474455	39.0	36.0	41.0	32.0	41.0
38	37.320526496152084	39.0	36.0	41.0	32.0	41.0
39	37.263861911374484	39.0	36.0	41.0	32.0	41.0
40	37.17441651660611	39.0	36.0	41.0	31.0	41.0
41	37.16983779150918	39.0	36.0	41.0	31.0	41.0
42	37.1266618446968	39.0	36.0	41.0	31.0	41.0
43	37.06020951959873	39.0	35.0	41.0	31.0	41.0
44	36.96395827922926	39.0	35.0	41.0	31.0	41.0
45	36.76966651857353	39.0	35.0	40.0	31.0	41.0
46	36.62819893702035	39.0	35.0	40.0	31.0	41.0
47	36.50294934284563	39.0	35.0	40.0	30.0	41.0
48	36.494310576354174	39.0	35.0	40.0	30.0	41.0
49	36.343903749475054	39.0	35.0	40.0	30.0	41.0
50	36.17358233012751	38.0	35.0	40.0	30.0	41.0
51	34.95682405318762	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	6.0
11	6.0
12	9.0
13	13.0
14	20.0
15	29.0
16	80.0
17	122.0
18	239.0
19	477.0
20	795.0
21	1254.0
22	1939.0
23	2746.0
24	3846.0
25	4921.0
26	6560.0
27	8708.0
28	11799.0
29	15804.0
30	21217.0
31	28292.0
32	37576.0
33	50300.0
34	73458.0
35	95129.0
36	123510.0
37	183599.0
38	308306.0
39	416880.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.853445741034463	32.18450258948367	19.758057304175438	27.20399436530643
2	23.784402143701858	30.34671680370648	20.398005679049973	25.470875373541695
3	20.49086794742193	30.796361916740178	20.728103141506217	27.98466699433168
4	20.419539995521436	31.507352073196643	19.774440554912864	28.298667376369057
5	18.669823125280356	33.58573555430118	18.35825519095072	29.386186129467745
6	21.430579730985592	35.35426812298742	19.855569840223957	23.359582305803034
7	87.33538946707216	3.7851748035619623	4.67108656723679	4.208349162129078
8	89.04268158758708	3.288096960227334	3.5476534391591175	4.121568013026473
9	80.986271694393	7.68510390486554	7.064686654232566	4.263937746508901
10	30.486439461742247	43.13502445683085	12.75811668451654	13.620419396910362
11	22.730150690135495	27.578877482616942	31.98575730324539	17.705214524002177
12	26.33725601297496	24.253578380977114	30.959836711216234	18.449328894831694
13	24.83128471156536	25.849979503050935	28.333508612653453	20.98522717273026
14	19.89792304737049	28.11945321437233	29.336464036618352	22.646159701638826
15	17.969492813190165	30.425413641309827	30.08043532228859	21.524658223211418
16	20.344563372059334	28.816707195538033	30.098320967198443	20.740408465204194
17	19.46909682501186	28.43316742609115	30.378696336805294	21.719039412091696
18	21.23176290216767	27.4578989804467	28.144850830144325	23.16548728724131
19	21.116793976687134	30.860034812619254	27.181529995299652	20.84164121539396
20	25.03868664994001	28.042401856100685	26.7528468581003	20.166064635859
21	22.737805746156912	29.71728518803894	26.13715141772353	21.40775764808062
22	21.7480856994253	26.94865889857337	27.253001032359425	24.05025436964191
23	21.097549022764134	30.519635218694933	26.51997576137402	21.862839997166912
24	19.922533694766447	28.116305340868198	28.98139821386796	22.9797627504974
25	21.0021827641048	29.365796494270512	25.859423123563335	23.772597618061354
26	23.35013868529063	29.546012252382187	25.46515196717054	21.63869709515664
27	21.395380781803002	28.586554716838048	26.38518954133337	23.632874960025585
28	21.05419421950265	29.49342845634722	28.000978702489466	21.451398621660662
29	22.046275171362364	28.703741462287404	25.560661310989154	23.689322055361078
30	23.517047523589376	26.980423803933267	27.273676837875215	22.22885183460214
31	21.605072082726114	29.344977603595446	25.00255764722211	24.047392666456332
32	22.68929987716139	27.702789230552398	25.812419648740242	23.795491243545968
33	21.56679680261903	29.198959198551407	25.40627242412731	23.827971574702257
34	19.944568809295387	27.054899629337896	28.840387789398676	24.16014377196804
35	20.919980340099116	26.91660782289491	26.877402489252518	25.286009347753456
36	20.09960157937399	30.61764855280093	26.6292212804834	22.653528587341683
37	21.01513197101953	29.939281812660028	25.226128208595266	23.819458007725167
38	20.928636992235482	27.977798906686296	27.712948276861198	23.38061582421702
39	20.474627781843783	26.879978022119534	26.958674859722887	25.686719336313796
40	22.385887796910648	27.001242694608337	28.76698510268864	21.845884405792372
41	18.776135398624522	26.514323897582504	30.975933791635097	23.733606912157875
42	22.754832880111092	27.331840955122054	26.80278357868861	23.110542586078243
43	21.8744298950685	27.691771673287935	27.09439113329885	23.33940729834472
44	23.356792145197097	27.10190310416099	25.947635124258728	23.59366962638319
45	23.05516862943734	26.49901378553967	26.725946848155886	23.719870736867108
46	19.526473973882666	26.866671102306604	28.197649253918204	25.409205669892522
47	20.428983616033836	28.628264040767824	27.654927244773635	23.2878250984247
48	21.937029652252985	25.495986818995124	29.12069161642589	23.446291912326
49	20.95002822354767	26.737035947999992	27.23704703709984	25.075888791352508
50	19.157600433261862	27.218589051552865	27.42749338409995	26.196317131085323
51	19.41658457155653	24.931658950799452	29.38661538494558	26.265141092698435
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	969.0
1	809.0
2	649.0
3	501.0
4	353.0
5	428.5
6	504.0
7	557.5
8	611.0
9	724.5
10	838.0
11	1003.5
12	1169.0
13	1368.0
14	1567.0
15	1847.0
16	2127.0
17	2232.0
18	2337.0
19	2550.0
20	2763.0
21	3738.0
22	4713.0
23	5882.0
24	7051.0
25	7698.0
26	10977.5
27	13610.0
28	17152.5
29	20695.0
30	24557.5
31	28420.0
32	35675.5
33	42931.0
34	45358.0
35	47785.0
36	54518.0
37	61251.0
38	63060.5
39	64870.0
40	67551.5
41	70233.0
42	74903.0
43	79573.0
44	89714.5
45	99856.0
46	110838.5
47	121821.0
48	129744.0
49	137667.0
50	131234.5
51	124802.0
52	115544.5
53	106287.0
54	93446.5
55	80606.0
56	71395.5
57	62185.0
58	58482.5
59	54780.0
60	51656.0
61	48532.0
62	43090.5
63	37649.0
64	30594.5
65	23540.0
66	18490.0
67	13440.0
68	10888.5
69	8337.0
70	6714.5
71	5092.0
72	3998.5
73	2905.0
74	2933.0
75	2446.0
76	1931.0
77	1542.5
78	1154.0
79	809.0
80	464.0
81	350.0
82	236.0
83	160.5
84	85.0
85	62.0
86	39.0
87	30.0
88	21.0
89	13.5
90	6.0
91	5.0
92	4.0
93	4.0
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1397769.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.73250208434449
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.19099864987497	19.565785239630372
2	10.428068538834324	5.575367279001515
3	3.8181296277896264	3.062044746995509
4	1.9031248514276309	2.035011562302278
5	1.2188274083616102	1.629115311724147
6	0.8831135362679727	1.4164700669397845
7	0.6512771905650405	1.2187188197986176
8	0.550694845663867	1.1777160887637674
9	0.48630557206523656	1.1700148246976048
>10	5.574699418442752	34.44708017923712
>50	1.1313652226514541	20.890379330683125
>100	0.15772357129254544	5.9645408102893605
>500	0.00378104450870233	0.680607719313271
>1k	0.0016204476465867127	0.7532386056640878
>5k	2.7007460776445213E-4	0.4139094149594763
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	5733	0.4101536090727438	RNA PCR Primer, Index 41 (96% over 27bp)
AATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	2493	0.17835565104105186	Illumina PCR Primer Index 11 (95% over 23bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	2450	0.17527932011655717	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCC	1714	0.12262398150195061	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGT	1607	0.11496892548053363	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.430851592788222E-4	0.0	0.0	0.45908873354610097	0.0
2	1.430851592788222E-4	0.0	0.0	0.6585494455807791	0.0
3	1.430851592788222E-4	0.0	0.0	0.9166750729197743	0.0
4	1.430851592788222E-4	0.0	0.0	2.030807665644323	0.0
5	1.430851592788222E-4	0.0	0.0	2.254235141858204	0.0
6	1.430851592788222E-4	0.0	0.0	3.465451015153434	0.0
7	1.430851592788222E-4	0.0	0.0	4.401585669735128	0.0
8	1.430851592788222E-4	0.0	0.0	5.283419506370509	0.0
9	1.430851592788222E-4	0.0	0.0	6.443553977803199	0.0
10	1.430851592788222E-4	0.0	0.0	8.090392618522802	0.0
11	1.430851592788222E-4	0.0	0.0	9.557659384347485	0.0
12	1.430851592788222E-4	0.0	0.0	10.250406182995903	0.0
13	1.430851592788222E-4	0.0	0.0	10.582149124783852	0.0
14	1.430851592788222E-4	0.0	0.0	10.941435959732974	0.0
15	1.430851592788222E-4	0.0	0.0	11.23948234651076	0.0
16	1.430851592788222E-4	0.0	0.0	11.748865513543368	0.0
17	1.430851592788222E-4	0.0	0.0	12.433313372953615	0.0
18	1.430851592788222E-4	0.0	0.0	13.17714157346457	0.0
19	1.430851592788222E-4	0.0	0.0	13.589513002506136	0.0
20	1.430851592788222E-4	0.0	0.0	14.014762095882796	0.0
21	1.430851592788222E-4	0.0	0.0	14.519351910079562	0.0
22	1.430851592788222E-4	0.0	0.0	15.102996274777878	0.0
23	1.430851592788222E-4	0.0	0.0	15.677554731861989	0.0
24	1.430851592788222E-4	0.0	0.0	16.080554082970792	0.0
25	1.430851592788222E-4	0.0	0.0	16.44298879142405	0.0
26	1.430851592788222E-4	0.0	0.0	16.78424689630404	0.0
27	1.430851592788222E-4	0.0	0.0	17.162492514857604	0.0
28	1.430851592788222E-4	0.0	0.0	17.547677763636194	0.0
29	1.430851592788222E-4	0.0	0.0	17.96491408809324	0.0
30	1.430851592788222E-4	0.0	0.0	18.54419435543355	0.0
31	2.1462773891823327E-4	0.0	0.0	18.973592918429297	0.0
32	2.1462773891823327E-4	0.0	0.0	19.42638590496713	0.0
33	2.1462773891823327E-4	0.0	0.0	19.831531533465114	0.0
34	2.1462773891823327E-4	0.0	0.0	20.223727955048368	0.0
35	2.1462773891823327E-4	0.0	0.0	20.656202848968608	0.0
36	2.1462773891823327E-4	0.0	0.0	21.069647416704765	0.0
37	2.1462773891823327E-4	0.0	0.0	21.48094570705174	0.0
38	2.1462773891823327E-4	0.0	0.0	21.864914731976455	0.0
39	2.1462773891823327E-4	0.0	0.0	22.2727074359211	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCATCCG	45	3.8562575E-10	45.000004	1
CGGCGTA	25	3.8912407E-5	45.0	35
TCGCACC	20	7.0338114E-4	45.0	41
CGAACCG	20	7.0338114E-4	45.0	1
CGTATAG	20	7.0338114E-4	45.0	1
TATCGAT	20	7.0338114E-4	45.0	13
CCGATAG	30	2.1658398E-6	44.999996	1
CGATAAG	30	2.1658398E-6	44.999996	1
ACGATAG	100	0.0	42.75	1
CGCAATG	100	0.0	42.75	1
CGCACCG	95	0.0	42.63158	1
CTTAACG	85	0.0	42.35294	1
ATTCACG	80	0.0	42.1875	1
CGAATAT	390	0.0	41.538464	13
ATATGCG	60	3.6379788E-12	41.249996	1
AATTGCG	60	3.6379788E-12	41.249996	1
CTAGACG	55	6.184564E-11	40.90909	1
CGTATAT	55	6.184564E-11	40.90909	45
CAAGCCG	55	6.184564E-11	40.90909	1
CACGTAT	55	6.184564E-11	40.90909	43
>>END_MODULE