FastQCFastQC Report
Sat 18 Jun 2016
SRR3553489_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553489_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473326
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT61340.41633691389414157Illumina PCR Primer Index 2 (96% over 25bp)
AATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT48170.32694732869711113Illumina PCR Primer Index 2 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGT28270.19187878310706524No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCC26880.18244434700806203No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG207.0339354E-445.01
CATTACG302.1659216E-644.9999961
ACGATAG650.041.538461
CGAAACG556.184564E-1140.909091
CGAGATA7650.040.019
TATCGCG451.9288564E-840.01
GTAACGG2200.039.886362
TTACGCG800.039.3751
TAGTACG403.4590994E-739.3751
GGCCGAT2500.038.78
CGCGTAG356.249489E-638.5714261
ACGGGAG14050.038.274025
ACTAACG659.094947E-1238.076921
TATACGG1600.037.968752
GTTACGG1900.037.8947372
AAACGCG950.037.8947371
CTTGACG950.037.8947371
CCACGCG950.037.8947371
ATACCGG1850.037.7027052
CTAGCGG3600.037.52
Produced by FastQC (version 0.11.5)