FastQCFastQC Report
Sat 18 Jun 2016
SRR3553486_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553486_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726793
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT26880.36984395832100747No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCC9520.13098640190535682No Hit
AATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT9340.1285097682558858No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGT8180.11254924029262803No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG253.8893842E-545.0000041
CCGATAG253.8893842E-545.0000041
CGCGACG406.8084773E-945.0000041
CCGCTAG253.8893842E-545.0000041
ATCACCG406.8084773E-945.0000041
GCCGCGA406.8084773E-945.00000415
ATCCGCG253.8893842E-545.0000041
GTTACCG207.0315716E-445.00000430
TCTACGT207.0315716E-445.00000433
CGAGCAA207.0315716E-445.00000438
CGTCGTA207.0315716E-445.00000445
CATAACG253.8893842E-545.0000041
TAGGCGA207.0315716E-445.00000414
CGCGTTG207.0315716E-445.0000041
GCTCGAT207.0315716E-445.00000431
TAGTCCG406.8084773E-945.0000041
TCGCGCG207.0315716E-445.0000041
ACGACCG207.0315716E-445.0000041
GTACGAA253.8893842E-545.0000049
TAACGCG351.2111013E-745.01