##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553486_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	726793
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.984515536060474	33.0	31.0	34.0	30.0	34.0
2	32.19381997350002	34.0	31.0	34.0	30.0	34.0
3	32.318768892931	34.0	31.0	34.0	30.0	34.0
4	35.93513558881277	37.0	35.0	37.0	35.0	37.0
5	35.74213015260191	37.0	35.0	37.0	35.0	37.0
6	35.74605286512116	37.0	35.0	37.0	33.0	37.0
7	35.889403172567704	37.0	35.0	37.0	35.0	37.0
8	36.052745417195815	37.0	35.0	37.0	35.0	37.0
9	37.67952085394328	39.0	38.0	39.0	35.0	39.0
10	37.172771339294684	39.0	37.0	39.0	34.0	39.0
11	37.13366942169228	39.0	37.0	39.0	34.0	39.0
12	37.13833650021395	39.0	37.0	39.0	33.0	39.0
13	37.13646939362377	39.0	37.0	39.0	33.0	39.0
14	38.361932489718534	40.0	38.0	41.0	34.0	41.0
15	38.3617811398844	40.0	38.0	41.0	34.0	41.0
16	38.25880959227731	40.0	38.0	41.0	33.0	41.0
17	38.28300079940231	40.0	38.0	41.0	33.0	41.0
18	38.2472987494376	40.0	38.0	41.0	33.0	41.0
19	38.25855780118961	40.0	38.0	41.0	34.0	41.0
20	38.205744964522225	40.0	37.0	41.0	34.0	41.0
21	38.17920783496814	40.0	37.0	41.0	34.0	41.0
22	38.18656618872224	40.0	37.0	41.0	34.0	41.0
23	38.06079585246418	40.0	37.0	41.0	33.0	41.0
24	37.99099468486901	40.0	37.0	41.0	33.0	41.0
25	37.99435327527921	40.0	37.0	41.0	33.0	41.0
26	37.93651975184131	40.0	37.0	41.0	33.0	41.0
27	37.87799001916639	40.0	37.0	41.0	33.0	41.0
28	37.822849146868506	40.0	37.0	41.0	33.0	41.0
29	37.72163463324495	40.0	37.0	41.0	33.0	41.0
30	37.69979072445662	40.0	37.0	41.0	33.0	41.0
31	37.63561289115333	40.0	37.0	41.0	33.0	41.0
32	37.58695116766397	40.0	37.0	41.0	32.0	41.0
33	37.48729005370167	40.0	37.0	41.0	32.0	41.0
34	37.44669114864893	40.0	36.0	41.0	32.0	41.0
35	37.371558339169475	40.0	36.0	41.0	32.0	41.0
36	37.30842344381413	40.0	36.0	41.0	31.0	41.0
37	37.215420346646155	39.0	36.0	41.0	31.0	41.0
38	37.18346489303006	39.0	36.0	41.0	31.0	41.0
39	37.110715155484435	39.0	36.0	41.0	31.0	41.0
40	37.004777151128316	39.0	36.0	41.0	31.0	41.0
41	36.96942595759728	39.0	36.0	41.0	31.0	41.0
42	36.93100649015607	39.0	35.0	41.0	31.0	41.0
43	36.850965818327914	39.0	35.0	41.0	31.0	41.0
44	36.70921431549286	39.0	35.0	41.0	31.0	41.0
45	36.512813139367054	39.0	35.0	40.0	30.0	41.0
46	36.36163804549576	39.0	35.0	40.0	30.0	41.0
47	36.27237466513849	39.0	35.0	40.0	30.0	41.0
48	36.23300994918773	39.0	35.0	40.0	30.0	41.0
49	36.05536652114151	38.0	35.0	40.0	29.0	41.0
50	35.8959745071843	38.0	35.0	40.0	29.0	41.0
51	34.69156830074038	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	1.0
12	2.0
13	4.0
14	8.0
15	15.0
16	22.0
17	67.0
18	119.0
19	228.0
20	419.0
21	780.0
22	1252.0
23	1695.0
24	2285.0
25	3066.0
26	4023.0
27	5107.0
28	6907.0
29	8807.0
30	11763.0
31	15333.0
32	19491.0
33	26396.0
34	38693.0
35	49677.0
36	62311.0
37	92999.0
38	156531.0
39	218730.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.557022425917694	31.440451407759845	20.788450081384934	27.214076084937528
2	24.516058905355447	29.18176151944226	20.212495167124615	26.08968440807768
3	20.55757278895091	30.872889529756065	21.192966910798535	27.376570770494485
4	20.315963417369183	31.622483980995963	20.045735168060233	28.015817433574618
5	19.103376064436503	33.67533809489085	17.396012344642838	29.82527349602982
6	20.498683944396824	36.658580916437	19.66006827253427	23.1826668666319
7	89.30740939992543	3.308920146451603	3.7754904078602847	3.6081800457626865
8	90.73890364931968	2.6859091928513346	2.940727277230243	3.6344598805987403
9	82.77542574020387	7.603127713117765	6.0798604279347765	3.541586118743576
10	30.889538011510844	45.45572123011642	11.682005743038252	11.97273501533449
11	22.042589843325402	28.407538322465957	31.761725828399555	17.788146005809082
12	26.177880084150505	24.319441711739106	30.877430024780097	18.625248179330292
13	24.841598639502582	25.056790585489953	29.190567327973717	20.91104344703375
14	19.91185936023049	29.28811917561121	29.29431075973489	21.505710704423407
15	19.638466523480552	30.54225893755168	29.38016739291655	20.439107146051214
16	22.213202383622296	29.21120594171931	29.566465279660097	19.0091263949983
17	20.6512720953559	28.343283438338013	29.808074651241824	21.197369815064263
18	22.226411096419476	28.022009017698302	27.044013907673847	22.707565978208375
19	21.90155931606386	30.49878025792764	27.740223144691818	19.859437281316687
20	26.292630776575997	28.40588723336631	26.652293018782515	18.649188971275176
21	24.29962864254334	29.116818681522798	26.589276451479304	19.994276224454556
22	22.338547564437192	26.640460213568375	27.45513509348604	23.56585712850839
23	22.012319876498534	30.608715273812486	26.297446453116635	21.08151839657234
24	20.884901202955998	28.381946441421423	28.20307845562629	22.530073899996285
25	22.650878585787147	28.70666063101874	25.273771211335276	23.368689571858837
26	23.79439537805125	29.678326566161207	25.85578011896097	20.67149793682658
27	21.468973971956252	28.91731208198208	26.701825691771937	22.911888254289735
28	22.125557070582683	30.30037438445334	27.180641530669668	20.393427014294303
29	23.886030823081676	28.847828749038584	26.313269390321594	20.95287103755815
30	23.94753389204354	26.38536694767286	26.62092232588921	23.046176834394387
31	23.528019669974807	29.33613835025929	24.30141732240129	22.834424657364615
32	24.131217554379308	27.98692337433079	26.04538018390381	21.836478887386093
33	23.012604689368224	29.414840264009147	25.323991838116218	22.24856320850641
34	23.060486273258	28.065074925047433	26.66811595598747	22.2063228457071
35	23.468993234662413	27.862128556549116	27.126843544172825	21.54203466461565
36	21.732597864866612	30.41306121550428	25.61348279358772	22.240858126041392
37	22.136701922005305	30.574455174994807	25.453877513955142	21.83496538904475
38	22.768931456411938	28.95143459004146	26.967375855298553	21.31225809824806
39	22.976693501450896	26.522407342943588	26.28313701425303	24.217762141352488
40	23.44794184864191	27.427754532583553	27.305711529967958	21.81859208880658
41	20.072015002896286	26.961459452691482	30.38182811336928	22.58469743104295
42	23.929234321189114	27.747515454881928	26.289741370651615	22.033508853277343
43	22.532137761370844	27.58213136340058	27.144867933510643	22.740862941717932
44	23.952899931617395	27.406015192771534	25.907239062566646	22.73384581304443
45	24.738130389258014	26.522269752185284	26.46805899341353	22.27154086514317
46	20.529091501981995	27.616529052976567	27.742011824549767	24.112367620491668
47	21.384218064841022	28.46367535185397	27.648862881177998	22.503243702127016
48	22.670554064224614	25.730022165871162	29.157545545980767	22.441878223923457
49	22.076849942143088	26.509336220904718	27.571674465769487	23.842139371182718
50	20.632972524501476	27.373406183053493	26.964211267857564	25.029410024587467
51	20.948055361017513	25.84270899692209	28.406437596399524	24.80279804566087
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	554.0
1	444.0
2	334.0
3	255.5
4	177.0
5	184.0
6	191.0
7	200.5
8	210.0
9	239.5
10	269.0
11	321.5
12	374.0
13	474.5
14	575.0
15	667.0
16	759.0
17	734.5
18	710.0
19	846.5
20	983.0
21	1515.5
22	2048.0
23	2779.5
24	3511.0
25	4467.5
26	6769.5
27	8115.0
28	9749.0
29	11383.0
30	13474.5
31	15566.0
32	19306.0
33	23046.0
34	24324.0
35	25602.0
36	29426.0
37	33250.0
38	34686.0
39	36122.0
40	37632.5
41	39143.0
42	39478.5
43	39814.0
44	42392.5
45	44971.0
46	48925.0
47	52879.0
48	56932.5
49	60986.0
50	65064.0
51	69142.0
52	63978.5
53	58815.0
54	53973.0
55	49131.0
56	42572.0
57	36013.0
58	33452.5
59	30892.0
60	28315.5
61	25739.0
62	23307.5
63	20876.0
64	16645.5
65	12415.0
66	9267.0
67	6119.0
68	4794.0
69	3469.0
70	2968.5
71	2468.0
72	2104.0
73	1740.0
74	1613.0
75	1103.5
76	721.0
77	524.5
78	328.0
79	292.0
80	256.0
81	191.5
82	127.0
83	80.0
84	33.0
85	23.0
86	13.0
87	9.0
88	5.0
89	5.5
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	726793.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.213798628332952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.51055333920074	20.27715194258671
2	9.211364640626012	5.0135244484428565
3	3.224807728132803	2.632778043854939
4	1.6394632919532974	1.7846409554304346
5	1.0577398737593768	1.4392559962822997
6	0.8048785650711635	1.3142281914064937
7	0.6522709344080485	1.2425538902066642
8	0.5646344595179967	1.2292678783952302
9	0.48476334775724733	1.187302691543604
>10	6.794344357504432	43.262549621310335
>50	0.9997774575576571	17.446982106125102
>100	0.05286062815756242	2.354361888374185
>500	0.0020331010829831696	0.44359547476740563
>1k	5.082752707457924E-4	0.3718068712737095
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT	2688	0.36984395832100747	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCC	952	0.13098640190535682	No Hit
AATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT	934	0.1285097682558858	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGT	818	0.11254924029262803	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7518151660789247E-4	0.0	0.0	0.45459986543623837	0.0
2	2.7518151660789247E-4	0.0	0.0	0.6214974552589252	0.0
3	2.7518151660789247E-4	0.0	0.0	0.8044931638031737	0.0
4	2.7518151660789247E-4	0.0	0.0	1.6597573174205036	0.0
5	2.7518151660789247E-4	0.0	0.0	1.8075297918389417	0.0
6	2.7518151660789247E-4	0.0	0.0	2.700356222473249	0.0
7	2.7518151660789247E-4	0.0	0.0	3.335062390529353	0.0
8	2.7518151660789247E-4	0.0	0.0	4.035124168779832	0.0
9	2.7518151660789247E-4	0.0	0.0	4.932766275954776	0.0
10	2.7518151660789247E-4	0.0	0.0	6.173559734339764	0.0
11	2.7518151660789247E-4	0.0	0.0	7.300565635607388	0.0
12	2.7518151660789247E-4	0.0	0.0	7.804973355549654	0.0
13	2.7518151660789247E-4	0.0	0.0	8.028007974760351	0.0
14	2.7518151660789247E-4	0.0	0.0	8.277460019565407	0.0
15	2.7518151660789247E-4	0.0	0.0	8.476278665314608	0.0
16	2.7518151660789247E-4	0.0	0.0	8.867586781931031	0.0
17	2.7518151660789247E-4	0.0	0.0	9.39400902320193	0.0
18	2.7518151660789247E-4	0.0	0.0	10.026238557608563	0.0
19	2.7518151660789247E-4	0.0	0.0	10.344623572323894	0.0
20	2.7518151660789247E-4	0.0	0.0	10.691352283249838	0.0
21	2.7518151660789247E-4	0.0	0.0	11.059957924746112	0.0
22	2.7518151660789247E-4	0.0	0.0	11.516208879281997	0.0
23	2.7518151660789247E-4	0.0	0.0	11.964066797561342	0.0
24	2.7518151660789247E-4	0.0	0.0	12.272407686920484	0.0
25	2.7518151660789247E-4	0.0	0.0	12.561761051633685	0.0
26	2.7518151660789247E-4	0.0	0.0	12.828549531985036	0.0
27	2.7518151660789247E-4	0.0	0.0	13.117352533665018	0.0
28	2.7518151660789247E-4	0.0	0.0	13.403541310937227	0.0
29	2.7518151660789247E-4	0.0	0.0	13.718624147453264	0.0
30	2.7518151660789247E-4	0.0	0.0	14.148320085636488	0.0
31	4.1277227491183873E-4	0.0	0.0	14.476336453433095	0.0
32	4.1277227491183873E-4	0.0	0.0	14.816048035685538	0.0
33	4.1277227491183873E-4	0.0	0.0	15.134983413434087	0.0
34	4.1277227491183873E-4	0.0	0.0	15.457220969381929	0.0
35	4.1277227491183873E-4	0.0	0.0	15.791566512060518	0.0
36	4.1277227491183873E-4	0.0	0.0	16.13059014052144	0.0
37	4.1277227491183873E-4	0.0	0.0	16.486124659978838	0.0
38	5.503630332157849E-4	0.0	0.0	16.819369476590996	0.0
39	5.503630332157849E-4	0.0	0.0	17.16843723040811	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCG	25	3.8893842E-5	45.000004	1
CCGATAG	25	3.8893842E-5	45.000004	1
CGCGACG	40	6.8084773E-9	45.000004	1
CCGCTAG	25	3.8893842E-5	45.000004	1
ATCACCG	40	6.8084773E-9	45.000004	1
GCCGCGA	40	6.8084773E-9	45.000004	15
ATCCGCG	25	3.8893842E-5	45.000004	1
GTTACCG	20	7.0315716E-4	45.000004	30
TCTACGT	20	7.0315716E-4	45.000004	33
CGAGCAA	20	7.0315716E-4	45.000004	38
CGTCGTA	20	7.0315716E-4	45.000004	45
CATAACG	25	3.8893842E-5	45.000004	1
TAGGCGA	20	7.0315716E-4	45.000004	14
CGCGTTG	20	7.0315716E-4	45.000004	1
GCTCGAT	20	7.0315716E-4	45.000004	31
TAGTCCG	40	6.8084773E-9	45.000004	1
TCGCGCG	20	7.0315716E-4	45.000004	1
ACGACCG	20	7.0315716E-4	45.000004	1
GTACGAA	25	3.8893842E-5	45.000004	9
TAACGCG	35	1.2111013E-7	45.0	1
>>END_MODULE