FastQCFastQC Report
Sat 18 Jun 2016
SRR3553483_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553483_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1184542
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT73460.6201553005296562TruSeq Adapter, Index 13 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT22590.19070661909835193No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCC15170.12806637502089413No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGT15090.12739100850792964No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC14600.12325438861602205TruSeq Adapter, Index 19 (95% over 21bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA13330.11253294522271055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG600.045.0000041
CGAAACG302.1655578E-645.0000041
CGCTCGC302.1655578E-645.00000445
CCGAACG302.1655578E-645.0000041
ATACCCG302.1655578E-645.0000041
TAATACG551.8189894E-1245.01
TCGCACG551.8189894E-1245.01
TCCGCGC207.033375E-445.026
CCGGATA207.033375E-445.05
CCGCTAA253.890878E-545.032
TACGCCC207.033375E-445.04
GTTAACG207.033375E-445.01
CGGATAT207.033375E-445.06
TTAAGCG253.890878E-545.01
ACGCTAG351.2119744E-745.01
CTCGAGT207.033375E-445.044
TTCGAGT207.033375E-445.013
GCGTAAG207.033375E-445.01
TGGTACG207.033375E-445.011
CGTAACG1150.044.9999961