##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553483_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1184542
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.854873022653482	33.0	31.0	34.0	30.0	34.0
2	32.057554734234834	34.0	31.0	34.0	30.0	34.0
3	32.19927870856415	34.0	31.0	34.0	30.0	34.0
4	35.79648589919142	37.0	35.0	37.0	35.0	37.0
5	35.622166204322006	37.0	35.0	37.0	33.0	37.0
6	35.627378345385814	37.0	35.0	37.0	33.0	37.0
7	35.80273388364448	37.0	35.0	37.0	35.0	37.0
8	35.949727405191204	37.0	35.0	37.0	35.0	37.0
9	37.56400110760108	39.0	37.0	39.0	35.0	39.0
10	37.07445915805434	39.0	37.0	39.0	33.0	39.0
11	37.09141507857045	39.0	37.0	39.0	33.0	39.0
12	37.078463237268075	39.0	37.0	39.0	33.0	39.0
13	37.04544203582482	39.0	37.0	39.0	33.0	39.0
14	38.28146574794309	40.0	38.0	41.0	33.0	41.0
15	38.26608849665103	40.0	38.0	41.0	33.0	41.0
16	38.23147005340461	40.0	38.0	41.0	33.0	41.0
17	38.231370436843946	40.0	38.0	41.0	33.0	41.0
18	38.19296994112492	40.0	37.0	41.0	33.0	41.0
19	38.18325732646036	40.0	37.0	41.0	34.0	41.0
20	38.140706703519164	40.0	37.0	41.0	33.0	41.0
21	38.12040180930689	40.0	37.0	41.0	33.0	41.0
22	38.12049720482685	40.0	37.0	41.0	33.0	41.0
23	38.02305785696075	40.0	37.0	41.0	33.0	41.0
24	37.92565143321216	40.0	37.0	41.0	33.0	41.0
25	37.9316140753135	40.0	37.0	41.0	33.0	41.0
26	37.8441726844637	40.0	37.0	41.0	33.0	41.0
27	37.775472714348666	40.0	37.0	41.0	33.0	41.0
28	37.70381885994756	40.0	37.0	41.0	33.0	41.0
29	37.60190352051679	40.0	37.0	41.0	32.0	41.0
30	37.584223269415524	40.0	37.0	41.0	32.0	41.0
31	37.47906026126554	40.0	36.0	41.0	32.0	41.0
32	37.46508017444717	39.0	36.0	41.0	32.0	41.0
33	37.386631288717496	39.0	36.0	41.0	32.0	41.0
34	37.33824803172872	39.0	36.0	41.0	32.0	41.0
35	37.296132175980254	39.0	36.0	41.0	31.0	41.0
36	37.223933807328066	39.0	36.0	41.0	31.0	41.0
37	37.14238583351202	39.0	36.0	41.0	31.0	41.0
38	37.11017760450875	39.0	36.0	41.0	31.0	41.0
39	37.12415600291083	39.0	36.0	41.0	31.0	41.0
40	37.03995974815583	39.0	35.0	41.0	31.0	41.0
41	37.011211928323355	39.0	35.0	41.0	31.0	41.0
42	36.9431180996537	39.0	35.0	41.0	31.0	41.0
43	36.87087245534561	39.0	35.0	40.0	31.0	41.0
44	36.778426598634745	39.0	35.0	40.0	31.0	41.0
45	36.57063151834211	39.0	35.0	40.0	30.0	41.0
46	36.42387775190749	39.0	35.0	40.0	30.0	41.0
47	36.33647941567289	38.0	35.0	40.0	30.0	41.0
48	36.31314719106625	38.0	35.0	40.0	30.0	41.0
49	36.16206516949167	38.0	35.0	40.0	30.0	41.0
50	36.00725765738994	38.0	35.0	40.0	29.0	41.0
51	34.84184182578583	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	5.0
11	6.0
12	14.0
13	10.0
14	21.0
15	33.0
16	52.0
17	103.0
18	174.0
19	351.0
20	663.0
21	1088.0
22	1706.0
23	2443.0
24	3243.0
25	4474.0
26	6018.0
27	7986.0
28	10836.0
29	14632.0
30	19794.0
31	26066.0
32	33661.0
33	44829.0
34	64747.0
35	84015.0
36	109155.0
37	161020.0
38	257277.0
39	329999.0
40	114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.454523351641395	31.434258979419894	21.317859560910463	27.793358108028247
2	24.405044312485334	29.765766009141085	20.198355144857675	25.630834533515905
3	20.330726981398715	30.023587175465284	21.221282149556536	28.424403693579457
4	20.394802379316225	31.64691501018959	19.744086744074927	28.214195866419256
5	18.40145811630149	33.10435594516699	17.91907758441659	30.57510835411492
6	20.895586648679405	35.732291467926004	19.707870214817202	23.66425166857739
7	86.52989931973708	3.5640779305419312	5.2876976924414665	4.618325057279522
8	87.62931158202917	3.2303624523233454	4.2440031674689465	4.896322798178536
9	79.1918733147495	7.706860541880321	8.350484828735494	4.750781314634686
10	29.362487780087154	42.88788409359904	12.990759297686363	14.758868828627437
11	22.88791786192469	27.70640466948407	31.472839291472994	17.932838177118246
12	26.575672285153253	23.88011569028367	30.955002017657456	18.589210006905624
13	24.893756405429272	25.323205086860572	28.290174599127766	21.49286390858239
14	18.85572651708424	27.790065696277548	29.49469077499996	23.859517011638253
15	17.587219364108662	30.361186011133416	29.689365172361974	22.362229452395948
16	19.14444570137657	27.661661638000172	31.161664170624597	22.032228489998666
17	18.82415313260315	27.903274008013224	30.69008950294713	22.582483356436498
18	20.62037479464637	27.26083161255574	27.880142704944188	24.2386508878537
19	20.190757271586822	31.2519944417336	27.49771641697804	21.05953186970154
20	25.038453680831918	27.61100914952783	27.37496855324674	19.97556861639351
21	21.905681689631944	29.812366298535636	25.661563709855795	22.62038830197663
22	20.559845070921927	26.934798428422123	26.661950357184466	25.843406143471487
23	21.301313081342833	30.336703975038454	25.16829289294934	23.19369005066937
24	18.74294030941917	28.618149461986153	28.91184947431159	23.72706075428309
25	21.073798987287915	29.283301056442067	25.449076520714335	24.193823435555682
26	23.24864800066186	29.309387088005323	25.146005798021516	22.295959113311305
27	20.844005531251742	28.873606845514978	25.689084895259096	24.593302727974187
28	20.86012990674877	31.201932898960106	26.271166408620378	21.66677078567075
29	20.844005531251742	29.82798414914794	25.079482196494514	24.24852812310581
30	22.693243464562673	27.945231152631145	26.01309197985382	23.348433402952367
31	21.299118140175697	29.841069375336627	22.766351889591082	26.093460594896595
32	20.870682508513838	29.003614899260644	25.965309799061576	24.16039279316394
33	19.948131851804327	29.366793241607304	24.260009353826202	26.425065552762167
34	18.259715569393066	29.545343263472297	26.657307212407833	25.537633954726807
35	18.989617928279454	27.956205858466816	26.083498938830367	26.970677274423366
36	17.7553012050227	31.354650151704206	25.577733841434075	25.312314801839026
37	18.709847350283905	30.699966738199237	24.229026915043956	26.3611589964729
38	19.574823011763197	28.019352627429	27.736627320939235	24.669197039868575
39	19.773718449831243	24.465320773767413	26.774314460778935	28.986646315622412
40	21.729664292190566	25.272299335945874	26.993217631793552	26.00481874007
41	18.075340511353755	25.81900852819064	29.300691744150903	26.804959216304695
42	21.985797042232356	27.73139323046376	25.03870694327428	25.24410278402961
43	20.92319225489683	27.454070856077706	26.364957933108325	25.257778955917136
44	22.91476368081503	26.773132569381247	25.237349118899964	25.074754630903755
45	23.251433887527835	25.17133204225768	26.61771385058529	24.959520219629187
46	18.216238850120973	27.222842246201484	28.363451865784413	26.197467037893123
47	20.005369163778067	27.797072623849555	26.82969451484202	25.367863697530353
48	20.824757585632252	25.00747124204967	28.944182646119764	25.22358852619831
49	20.213297628957015	26.242801015075866	26.887185089258125	26.65671626670899
50	18.366423478441458	25.884772342390562	27.151000133384883	28.5978040457831
51	18.669072097063673	23.55501113510538	29.455941621318622	28.31997514651232
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	880.0
1	737.5
2	595.0
3	444.0
4	293.0
5	316.5
6	340.0
7	390.5
8	441.0
9	534.0
10	627.0
11	829.5
12	1032.0
13	1203.0
14	1374.0
15	1423.0
16	1472.0
17	1487.0
18	1502.0
19	1912.0
20	2322.0
21	2716.5
22	3111.0
23	3797.5
24	4484.0
25	5672.0
26	9056.0
27	11252.0
28	13708.5
29	16165.0
30	19512.5
31	22860.0
32	26776.5
33	30693.0
34	34785.0
35	38877.0
36	40918.5
37	42960.0
38	47403.0
39	51846.0
40	55193.5
41	58541.0
42	61523.5
43	64506.0
44	74220.5
45	83935.0
46	95650.0
47	107365.0
48	116806.5
49	126248.0
50	121612.5
51	116977.0
52	103548.5
53	90120.0
54	78943.5
55	67767.0
56	59398.0
57	51029.0
58	47842.5
59	44656.0
60	44072.5
61	43489.0
62	39359.0
63	35229.0
64	28739.0
65	22249.0
66	16798.0
67	11347.0
68	9199.0
69	7051.0
70	5769.0
71	4487.0
72	3901.5
73	3316.0
74	3102.5
75	2352.0
76	1815.0
77	1374.0
78	933.0
79	606.0
80	279.0
81	215.5
82	152.0
83	129.0
84	106.0
85	69.0
86	32.0
87	21.5
88	11.0
89	11.5
90	12.0
91	7.5
92	3.0
93	4.5
94	6.0
95	5.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1184542.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.556723494023593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.54924431774239	21.003254331761553
2	10.462788036590512	5.975658898749866
3	3.8607622394822587	3.307521592471865
4	1.961311132361903	2.240344787704367
5	1.2407404076197395	1.7715740374129512
6	0.8893770730415415	1.5238617094062796
7	0.6890768187810806	1.3774443326050907
8	0.5405852393774999	1.2349874564683057
9	0.43561509075898536	1.1195765726965503
>10	5.251088248682678	33.939900761342834
>50	1.0031479844859754	19.455170527770008
>100	0.1100030735563687	4.8304080067425055
>500	0.0038754470356141767	0.7208878059837284
>1k	0.002086779173023018	0.8740381558850896
>5k	2.981113104318598E-4	0.6253710229991106
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	7346	0.6201553005296562	TruSeq Adapter, Index 13 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT	2259	0.19070661909835193	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCC	1517	0.12806637502089413	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGT	1509	0.12739100850792964	No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	1460	0.12325438861602205	TruSeq Adapter, Index 19 (95% over 21bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1333	0.11253294522271055	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6990887617323827	0.0
2	0.0	0.0	0.0	0.9867948962552615	0.0
3	0.0	0.0	0.0	1.3015156912967205	0.0
4	0.0	0.0	0.0	2.756424001850504	0.0
5	0.0	0.0	0.0	3.0149205346876684	0.0
6	0.0	0.0	0.0	4.525884265817506	0.0
7	0.0	0.0	0.0	5.654506129795314	0.0
8	0.0	0.0	0.0	6.7466582020730375	0.0
9	0.0	0.0	0.0	8.167798186978596	0.0
10	0.0	0.0	0.0	10.193222359359146	0.0
11	0.0	0.0	0.0	12.018400360645717	0.0
12	0.0	0.0	0.0	12.89578588180073	0.0
13	0.0	0.0	0.0	13.286232147108334	0.0
14	0.0	0.0	0.0	13.69660172454839	0.0
15	0.0	0.0	0.0	14.045344107680437	0.0
16	0.0	0.0	0.0	14.655115648073263	0.0
17	0.0	0.0	0.0	15.495356011015227	0.0
18	0.0	0.0	0.0	16.382872029864707	0.0
19	0.0	0.0	0.0	16.87943525852186	0.0
20	0.0	0.0	0.0	17.375069858223686	0.0
21	0.0	0.0	0.0	17.938494371664323	0.0
22	0.0	0.0	0.0	18.61926381673254	0.0
23	0.0	0.0	0.0	19.287876664567403	0.0
24	0.0	0.0	0.0	19.738008445458245	0.0
25	0.0	0.0	0.0	20.13951383741564	0.0
26	0.0	0.0	0.0	20.53477208912812	0.0
27	0.0	0.0	0.0	20.95603195158973	0.0
28	0.0	0.0	0.0	21.375856660211287	0.0
29	0.0	0.0	0.0	21.838482721591973	0.0
30	0.0	0.0	0.0	22.47071019854087	0.0
31	0.0	0.0	0.0	22.925569545022466	0.0
32	0.0	0.0	0.0	23.43445821254122	0.0
33	0.0	0.0	0.0	23.868803301191516	0.0
34	0.0	0.0	0.0	24.275036258739664	0.0
35	0.0	0.0	0.0	24.749143550840746	0.0
36	0.0	0.0	0.0	25.187794101011193	0.0
37	0.0	0.0	0.0	25.62821748827817	0.0
38	0.0	0.0	0.0	26.027612359882554	0.0
39	0.0	0.0	0.0	26.467444801450686	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCG	60	0.0	45.000004	1
CGAAACG	30	2.1655578E-6	45.000004	1
CGCTCGC	30	2.1655578E-6	45.000004	45
CCGAACG	30	2.1655578E-6	45.000004	1
ATACCCG	30	2.1655578E-6	45.000004	1
TAATACG	55	1.8189894E-12	45.0	1
TCGCACG	55	1.8189894E-12	45.0	1
TCCGCGC	20	7.033375E-4	45.0	26
CCGGATA	20	7.033375E-4	45.0	5
CCGCTAA	25	3.890878E-5	45.0	32
TACGCCC	20	7.033375E-4	45.0	4
GTTAACG	20	7.033375E-4	45.0	1
CGGATAT	20	7.033375E-4	45.0	6
TTAAGCG	25	3.890878E-5	45.0	1
ACGCTAG	35	1.2119744E-7	45.0	1
CTCGAGT	20	7.033375E-4	45.0	44
TTCGAGT	20	7.033375E-4	45.0	13
GCGTAAG	20	7.033375E-4	45.0	1
TGGTACG	20	7.033375E-4	45.0	11
CGTAACG	115	0.0	44.999996	1
>>END_MODULE