SRR3553480_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553480_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	972703
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	6515	0.6697830684186231	TruSeq Adapter, Index 21 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	6422	0.6602220821771908	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC	4959	0.5098164599060556	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGT	4929	0.5067322707959161	No Hit
AATGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT	1537	0.15801328874281256	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT	1281	0.13169487500295568	No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1225	0.125937721997362	TruSeq Adapter, Index 15 (95% over 22bp)
AACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	1214	0.12480685265697751	TruSeq Adapter, Index 15 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	35	1.2116652E-7	45.000004	2
AAGCGGT	45	3.8380676E-10	45.000004	3
CCGTAGG	45	3.8380676E-10	45.000004	2
CGTAACG	35	1.2116652E-7	45.000004	1
GTGTTCG	20	7.0327515E-4	45.0	38
CTTCGCG	20	7.0327515E-4	45.0	1
GATCGTG	25	3.890362E-5	45.0	8
GGTAATC	25	3.890362E-5	45.0	8
CGAAACG	25	3.890362E-5	45.0	1
CGCATAC	25	3.890362E-5	45.0	33
TGCGCGA	20	7.0327515E-4	45.0	11
CCAAGCG	20	7.0327515E-4	45.0	1
TTACGCT	20	7.0327515E-4	45.0	35
GTAAGCG	25	3.890362E-5	45.0	1
GATTCGA	25	3.890362E-5	45.0	9
TATCCGG	80	0.0	45.0	2
ATTAGCG	30	2.1651558E-6	44.999996	1
GGTAACG	30	2.1651558E-6	44.999996	1
CGATTAG	30	2.1651558E-6	44.999996	1
TAACACG	95	0.0	42.63158	1