##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553480_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	972703
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.899865632161102	33.0	31.0	34.0	30.0	34.0
2	32.10909907751904	34.0	31.0	34.0	30.0	34.0
3	32.23978233849387	34.0	31.0	34.0	30.0	34.0
4	35.85350204533141	37.0	35.0	37.0	35.0	37.0
5	35.64530899976663	37.0	35.0	37.0	33.0	37.0
6	35.649544619477886	37.0	35.0	37.0	33.0	37.0
7	35.74185953985955	37.0	35.0	37.0	35.0	37.0
8	35.96449070271193	37.0	35.0	37.0	35.0	37.0
9	37.6113942282485	39.0	37.0	39.0	35.0	39.0
10	37.105437116982266	39.0	37.0	39.0	33.0	39.0
11	37.0849262313368	39.0	37.0	39.0	33.0	39.0
12	37.07560375571988	39.0	37.0	39.0	33.0	39.0
13	37.08708824790301	39.0	37.0	39.0	33.0	39.0
14	38.28342772665449	40.0	38.0	41.0	33.0	41.0
15	38.27434890197728	40.0	38.0	41.0	33.0	41.0
16	38.17256757715356	40.0	37.0	41.0	33.0	41.0
17	38.17360283663153	40.0	37.0	41.0	33.0	41.0
18	38.14524783001595	40.0	37.0	41.0	33.0	41.0
19	38.14579064729933	40.0	37.0	41.0	33.0	41.0
20	38.133451834732696	40.0	37.0	41.0	33.0	41.0
21	38.095324060890114	40.0	37.0	41.0	33.0	41.0
22	38.122202768984984	40.0	37.0	41.0	33.0	41.0
23	38.0126739611166	40.0	37.0	41.0	33.0	41.0
24	37.918730588884785	40.0	37.0	41.0	33.0	41.0
25	37.9531964021906	40.0	37.0	41.0	33.0	41.0
26	37.87949764727774	40.0	37.0	41.0	33.0	41.0
27	37.84227970922265	40.0	37.0	41.0	33.0	41.0
28	37.80161878805761	40.0	37.0	41.0	33.0	41.0
29	37.670926274515445	40.0	37.0	41.0	33.0	41.0
30	37.69118117246477	40.0	37.0	41.0	33.0	41.0
31	37.52927049674978	40.0	37.0	41.0	32.0	41.0
32	37.50338489754838	40.0	37.0	41.0	32.0	41.0
33	37.47826520530933	40.0	36.0	41.0	32.0	41.0
34	37.34035157699729	39.0	36.0	41.0	32.0	41.0
35	37.32248281335618	39.0	36.0	41.0	31.0	41.0
36	37.17200728279855	39.0	36.0	41.0	31.0	41.0
37	37.16349183666546	39.0	36.0	41.0	31.0	41.0
38	37.13065550327284	39.0	36.0	41.0	31.0	41.0
39	37.023904521729655	39.0	35.0	41.0	31.0	41.0
40	36.926356760491124	39.0	35.0	41.0	31.0	41.0
41	36.87557558679268	39.0	35.0	41.0	31.0	41.0
42	36.85687820434398	39.0	35.0	41.0	31.0	41.0
43	36.81183465045343	39.0	35.0	40.0	31.0	41.0
44	36.710440905394556	39.0	35.0	40.0	31.0	41.0
45	36.53942570342643	39.0	35.0	40.0	30.0	41.0
46	36.35317255112815	39.0	35.0	40.0	30.0	41.0
47	36.28786484672094	39.0	35.0	40.0	30.0	41.0
48	36.23302282402748	38.0	35.0	40.0	29.0	41.0
49	36.09476582266118	38.0	35.0	40.0	29.0	41.0
50	35.96928353258908	38.0	35.0	40.0	29.0	41.0
51	34.67942115938781	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	9.0
13	11.0
14	10.0
15	25.0
16	37.0
17	90.0
18	172.0
19	359.0
20	651.0
21	1092.0
22	1593.0
23	2298.0
24	3034.0
25	3800.0
26	5140.0
27	6814.0
28	9041.0
29	12037.0
30	16204.0
31	21426.0
32	27825.0
33	36984.0
34	53136.0
35	68088.0
36	87489.0
37	127389.0
38	207627.0
39	280221.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.782708596560305	33.40824485994183	20.260141070809897	26.548905472687963
2	22.222404988984305	31.409690316571453	20.203597603790673	26.164307090653573
3	20.108912998109393	30.11114389489906	22.81446649182741	26.965476615164135
4	21.42586174813895	30.364972658663536	19.674042333579724	28.535123259617784
5	18.51120023275347	34.012129087707144	18.615240212068844	28.861430467470544
6	21.183238871474643	34.76796103229866	20.64001036287541	23.40878973335129
7	85.41600056749078	4.672238082950294	5.263785554275046	4.647975795283863
8	87.17810061241715	2.7820413836494797	4.001015726280273	6.038842277653097
9	79.81675804433624	7.492626217869175	8.060117014134839	4.63049872365974
10	29.284786825989023	44.1778220073342	12.545350430707009	13.992040735969766
11	20.388340531488026	26.004032063229985	35.13240937881347	18.47521802646851
12	23.372499108155314	23.71576935611384	34.08522436961744	18.826507166113398
13	23.748358954377647	25.648322252527233	29.696114846977956	20.907203946117157
14	18.99233373393523	27.626212728859684	31.26226607710678	22.119187460098303
15	18.443245266026732	30.696522988003533	29.075473191714224	21.78475855425551
16	21.272885968276032	29.27234726324479	28.958068392921582	20.496698375557596
17	19.766876425794923	28.81136379758261	30.44649805747489	20.97526171914757
18	22.100476712830126	27.099433228847857	27.4032258561966	23.396864202125418
19	20.666842808133623	30.139929659927027	28.778774199318807	20.414453332620543
20	23.751751562398802	29.204495102821724	27.758627247988336	19.285126086791138
21	21.717009200136115	29.689740856150337	26.962495232357668	21.63075471135588
22	21.79452515310429	27.078255130291566	28.04175580829914	23.085463908305
23	21.43059083810783	28.876645800413897	26.53245646410055	23.16030689737772
24	18.43142254110453	29.41000490386069	29.30586211824164	22.852710436793142
25	21.53051856527635	28.414634271715006	26.30031983041072	23.754527332597924
26	23.290562484129275	29.9705048714767	25.417213681874117	21.321718962519906
27	20.422883449521592	27.53378986185917	28.20953569589073	23.83379099272851
28	22.291799243962444	28.769316019381048	27.46912469684991	21.4697600398066
29	21.550565794492254	30.648409637885354	25.83779427019347	21.963230297428918
30	23.546036148752496	25.760997961351002	28.846934778652887	21.846031111243615
31	23.124119078485418	30.348832068987143	24.670120273094664	21.856928579432775
32	22.552721642680243	28.886618011870013	26.499764059533078	22.060896285916666
33	23.793696534296696	27.96639878770807	26.547157765525554	21.692746912469683
34	21.15270539928426	28.45308382928808	28.76674586178926	21.627464909638398
35	23.659123082790945	28.37690435826763	27.613978778722796	20.34999378021863
36	20.58603705344797	31.74853989347211	28.712464133450805	18.952958919629115
37	21.68041015602913	30.37679538358574	26.53862484232083	21.4041696180643
38	23.567008634701445	29.464183825895468	27.695504177534147	19.273303361868937
39	22.434185974547216	27.00649633032899	28.42368122643808	22.135636468685714
40	25.686463391189296	26.97287866902847	27.117321525686673	20.223336414095566
41	21.93218279372018	24.84067593088538	31.32898736818947	21.89815390720497
42	25.238022294575014	26.615215538555965	26.535643459514365	21.61111870735466
43	22.45392478485211	26.817641150484782	28.187843565816085	22.540590498847028
44	23.218803684166698	26.048547192719667	26.77374285881713	23.958906264296502
45	25.31471579711382	26.274001416670867	26.60534613340352	21.805936652811802
46	20.62232767864394	27.240791896395923	29.44413659667956	22.692743828280577
47	22.274630591249334	27.494517853856728	27.385440365661463	22.84541118923248
48	21.723485997267407	25.041559448259132	31.284369432396115	21.950585122077346
49	22.6586121354617	25.60380712303756	28.265565131391597	23.47201561010915
50	20.8461370017364	24.94245417151998	27.977913093719252	26.233495733024366
51	20.89620367162433	23.51509145134743	30.744430725514366	24.844274151513872
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1020.0
1	831.5
2	643.0
3	460.0
4	277.0
5	295.0
6	313.0
7	356.0
8	399.0
9	467.5
10	536.0
11	617.0
12	698.0
13	898.5
14	1099.0
15	973.0
16	847.0
17	1184.0
18	1521.0
19	1804.0
20	2087.0
21	2275.0
22	2463.0
23	3454.5
24	4446.0
25	5400.0
26	8100.5
27	9847.0
28	11533.5
29	13220.0
30	16017.5
31	18815.0
32	21990.5
33	25166.0
34	27985.0
35	30804.0
36	35114.5
37	39425.0
38	42899.0
39	46373.0
40	50271.0
41	54169.0
42	67900.0
43	81631.0
44	86510.5
45	91390.0
46	96156.5
47	100923.0
48	95419.5
49	89916.0
50	83975.5
51	78035.0
52	70645.5
53	63256.0
54	56776.0
55	50296.0
56	44377.5
57	38459.0
58	35811.5
59	33164.0
60	30387.0
61	27610.0
62	23854.5
63	20099.0
64	16895.5
65	13692.0
66	10821.5
67	7951.0
68	6269.0
69	4587.0
70	3746.0
71	2905.0
72	2901.0
73	2897.0
74	2533.5
75	1961.5
76	1753.0
77	1229.0
78	705.0
79	523.0
80	341.0
81	232.0
82	123.0
83	154.5
84	186.0
85	116.5
86	47.0
87	33.5
88	20.0
89	17.0
90	14.0
91	7.0
92	0.0
93	1.5
94	3.0
95	3.5
96	4.0
97	3.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	972703.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.161002296324906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.39823675137815	20.66967913701759
2	10.563803282874707	5.949745770139183
3	3.9361155432295605	3.3253487655446343
4	1.9060878504492869	2.1470937733399746
5	1.1744825031913593	1.653730223468265
6	0.8072040066781994	1.3639004331400462
7	0.6448097195141533	1.2710941594351481
8	0.5120422133713065	1.153569755725172
9	0.4321222192891906	1.095209532872632
>10	5.631023193425813	36.96695124664471
>50	0.9020696779335946	16.954071903597864
>100	0.08503866521998751	3.792413727144376
>500	0.004032005678533827	0.7584827454288676
>1k	0.002199275824654815	1.563312626499755
>5k	7.33091941551605E-4	1.3353962000018047
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	6515	0.6697830684186231	TruSeq Adapter, Index 21 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	6422	0.6602220821771908	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC	4959	0.5098164599060556	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGT	4929	0.5067322707959161	No Hit
AATGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT	1537	0.15801328874281256	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT	1281	0.13169487500295568	No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1225	0.125937721997362	TruSeq Adapter, Index 15 (95% over 22bp)
AACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	1214	0.12480685265697751	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7750567233780506	0.0
2	0.0	0.0	0.0	1.0801858326745162	0.0
3	0.0	0.0	0.0	1.517832267403308	0.0
4	0.0	0.0	0.0	3.4477121999212503	0.0
5	0.0	0.0	0.0	3.7341305619495366	0.0
6	0.0	0.0	0.0	5.1658111468762815	0.0
7	0.0	0.0	0.0	6.208267066103425	0.0
8	0.0	0.0	0.0	7.140206208883904	0.0
9	0.0	0.0	0.0	8.824893107145758	0.0
10	0.0	0.0	0.0	10.603853385874208	0.0
11	0.0	0.0	0.0	12.56663133556697	0.0
12	0.0	0.0	0.0	13.309818104806915	0.0
13	0.0	0.0	0.0	13.66491107768764	0.0
14	0.0	0.0	0.0	14.055472225334968	0.0
15	0.0	0.0	0.0	14.380237338632655	0.0
16	0.0	0.0	0.0	14.969523071276639	0.0
17	0.0	0.0	0.0	15.777375005525839	0.0
18	0.0	0.0	0.0	16.75249279584827	0.0
19	0.0	0.0	0.0	17.236196454621812	0.0
20	0.0	0.0	0.0	17.71732995580357	0.0
21	0.0	0.0	0.0	18.249763802517315	0.0
22	0.0	0.0	0.0	18.896518258913563	0.0
23	0.0	0.0	0.0	19.49300043281454	0.0
24	0.0	0.0	0.0	19.926431809092808	0.0
25	0.0	0.0	0.0	20.306198294854646	0.0
26	0.0	0.0	0.0	20.669104546814392	0.0
27	0.0	0.0	0.0	21.08845145948969	0.0
28	0.0	0.0	0.0	21.49525600311709	0.0
29	0.0	0.0	0.0	21.90792050605375	0.0
30	0.0	0.0	0.0	22.51972081920175	0.0
31	0.0	0.0	0.0	22.941843502076175	0.0
32	0.0	0.0	0.0	23.376919779213182	0.0
33	0.0	0.0	0.0	23.78845341280946	0.0
34	0.0	0.0	0.0	24.186519420624794	0.0
35	0.0	0.0	0.0	24.634138066809705	0.0
36	0.0	0.0	0.0	25.05153165971525	0.0
37	0.0	0.0	0.0	25.46656070763635	0.0
38	0.0	0.0	0.0	25.88148694925378	0.0
39	0.0	0.0	0.0	26.298983348462993	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	35	1.2116652E-7	45.000004	2
AAGCGGT	45	3.8380676E-10	45.000004	3
CCGTAGG	45	3.8380676E-10	45.000004	2
CGTAACG	35	1.2116652E-7	45.000004	1
GTGTTCG	20	7.0327515E-4	45.0	38
CTTCGCG	20	7.0327515E-4	45.0	1
GATCGTG	25	3.890362E-5	45.0	8
GGTAATC	25	3.890362E-5	45.0	8
CGAAACG	25	3.890362E-5	45.0	1
CGCATAC	25	3.890362E-5	45.0	33
TGCGCGA	20	7.0327515E-4	45.0	11
CCAAGCG	20	7.0327515E-4	45.0	1
TTACGCT	20	7.0327515E-4	45.0	35
GTAAGCG	25	3.890362E-5	45.0	1
GATTCGA	25	3.890362E-5	45.0	9
TATCCGG	80	0.0	45.0	2
ATTAGCG	30	2.1651558E-6	44.999996	1
GGTAACG	30	2.1651558E-6	44.999996	1
CGATTAG	30	2.1651558E-6	44.999996	1
TAACACG	95	0.0	42.63158	1
>>END_MODULE