##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553479_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	980388
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.880188251998188	33.0	31.0	34.0	30.0	34.0
2	32.0825142698605	34.0	31.0	34.0	30.0	34.0
3	32.21611545632953	34.0	31.0	34.0	30.0	34.0
4	35.80213752106309	37.0	35.0	37.0	35.0	37.0
5	35.62743322031685	37.0	35.0	37.0	33.0	37.0
6	35.639605951929234	37.0	35.0	37.0	33.0	37.0
7	35.75968086104685	37.0	35.0	37.0	35.0	37.0
8	35.970754435998806	37.0	35.0	37.0	35.0	37.0
9	37.62220467814784	39.0	37.0	39.0	35.0	39.0
10	37.098938379498726	39.0	37.0	39.0	33.0	39.0
11	37.076494204335425	39.0	37.0	39.0	33.0	39.0
12	37.076762465472854	39.0	37.0	39.0	33.0	39.0
13	37.08102914356357	39.0	37.0	39.0	33.0	39.0
14	38.27378038082882	40.0	38.0	41.0	33.0	41.0
15	38.264705402350906	40.0	38.0	41.0	33.0	41.0
16	38.175480524037425	40.0	37.0	41.0	33.0	41.0
17	38.15612084195237	40.0	37.0	41.0	33.0	41.0
18	38.109259803261565	40.0	37.0	41.0	33.0	41.0
19	38.11336940068626	40.0	37.0	41.0	33.0	41.0
20	38.09558154526575	40.0	37.0	41.0	33.0	41.0
21	38.07971231798023	40.0	37.0	41.0	33.0	41.0
22	38.09342219611011	40.0	37.0	41.0	33.0	41.0
23	37.99600668306834	40.0	37.0	41.0	33.0	41.0
24	37.92031624214086	40.0	37.0	41.0	33.0	41.0
25	37.951517154432736	40.0	37.0	41.0	33.0	41.0
26	37.86103869080405	40.0	37.0	41.0	33.0	41.0
27	37.81935519406602	40.0	37.0	41.0	33.0	41.0
28	37.7843833257853	40.0	37.0	41.0	33.0	41.0
29	37.63856248750495	40.0	37.0	41.0	33.0	41.0
30	37.68030004447219	40.0	37.0	41.0	33.0	41.0
31	37.53113461201075	40.0	37.0	41.0	32.0	41.0
32	37.507664312496686	40.0	36.0	41.0	32.0	41.0
33	37.468115684810506	40.0	36.0	41.0	32.0	41.0
34	37.31775480728038	39.0	36.0	41.0	31.0	41.0
35	37.304190789768946	39.0	36.0	41.0	31.0	41.0
36	37.19006658588233	39.0	36.0	41.0	31.0	41.0
37	37.14149295993015	39.0	36.0	41.0	31.0	41.0
38	37.088088593495634	39.0	36.0	41.0	31.0	41.0
39	36.999563438148975	39.0	35.0	41.0	31.0	41.0
40	36.926127206779356	39.0	35.0	41.0	31.0	41.0
41	36.89584531838415	39.0	35.0	41.0	31.0	41.0
42	36.85404452114877	39.0	35.0	41.0	31.0	41.0
43	36.803233005707945	39.0	35.0	40.0	31.0	41.0
44	36.759837941712874	39.0	35.0	40.0	31.0	41.0
45	36.58945845930387	39.0	35.0	40.0	30.0	41.0
46	36.38184779903467	39.0	35.0	40.0	30.0	41.0
47	36.3095152123445	39.0	35.0	40.0	30.0	41.0
48	36.26934846203748	38.0	35.0	40.0	30.0	41.0
49	36.132846383268664	38.0	35.0	40.0	29.0	41.0
50	35.985225237355	38.0	35.0	40.0	29.0	41.0
51	34.751273985401696	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	2.0
12	5.0
13	2.0
14	9.0
15	22.0
16	43.0
17	87.0
18	180.0
19	360.0
20	667.0
21	1135.0
22	1571.0
23	2210.0
24	3093.0
25	4010.0
26	5188.0
27	6855.0
28	9115.0
29	12218.0
30	16086.0
31	21479.0
32	28212.0
33	37530.0
34	53929.0
35	68645.0
36	88381.0
37	128259.0
38	210286.0
39	280715.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.76186979032791	33.38484355173666	20.357246314724375	26.496040343211053
2	22.28882850463286	31.50854559623333	20.08439515783547	26.118230741298344
3	20.154061453220564	30.173257934613645	22.729572373386866	26.943108238778933
4	21.448752942712478	30.410918942296316	19.56001093444636	28.580317180544846
5	18.580194780025867	34.013778218419645	18.561630701314176	28.84439630024031
6	21.200177888754247	34.811217599562625	20.58399327613149	23.404611235551638
7	85.58101486350303	4.662133767447174	5.172747932451234	4.584103436598571
8	87.28309608032738	2.8397940407267326	3.9636348058115765	5.91347507313431
9	80.01893128026862	7.488667751951268	7.873209382407781	4.619191585372322
10	29.30758026414032	44.30888586967609	12.528509120878672	13.85502474530492
11	20.425484604054724	26.13098079535857	35.034394545832875	18.40914005475383
12	23.457141458279786	23.7406006601468	34.021836252585715	18.780421628987707
13	23.829851038568403	25.58854249542018	29.630207632080356	20.951398833931055
14	18.89976213499145	27.69842144130691	31.263846558709407	22.137969864992225
15	18.569688735480238	30.62807786305014	29.109597424693078	21.692635976776543
16	21.383370665491622	29.24127998302713	28.95200675650865	20.423342594972603
17	19.73830769042461	28.814918175253062	30.456309134750732	20.990464999571596
18	22.02740139618192	27.156391143098446	27.445358368319482	23.37084909240015
19	20.745051959020305	30.116749695018708	28.670383562426306	20.46781478353468
20	23.82250700742971	29.259742061306337	27.62396112559517	19.293789805668776
21	21.824828537272996	29.634185648947152	27.076626804897653	21.464359008882198
22	22.01577334687899	27.004410498700516	27.977494624577208	23.002321529843286
23	21.399486733823753	28.964552809703914	26.551732579346137	23.0842278771262
24	18.484110372627978	29.392546624397685	29.29513621137754	22.8282067915968
25	21.515461225555597	28.509732881267418	26.265723366667075	23.70908252650991
26	23.30944483204609	29.863584621598793	25.42156778744742	21.405402758907698
27	20.439458663304734	27.550010812045844	28.23555571875625	23.774974805893176
28	22.194988106749573	28.759021938253014	27.45749641978482	21.58849353521259
29	21.51015720306654	30.6670420282582	25.881895739237937	21.940905029437324
30	23.53149977355904	25.773367279077263	28.94027670677324	21.75485624059046
31	23.126354055741196	30.35961272475795	24.63565445517489	21.878378764325962
32	22.52914152356006	28.85163833094652	26.576722685304187	22.04249746018923
33	23.843315095656006	27.951790515591785	26.471050237253007	21.733844151499202
34	21.09960546232716	28.42476652101005	28.838378274723887	21.637249741938906
35	23.59749405337479	28.425888525767352	27.623553123865246	20.353064296992617
36	20.64131751918628	31.826480944279208	28.630501393325908	18.901700143208608
37	21.645715777834898	30.42754501279085	26.469724231630742	21.457014977743505
38	23.60932610354268	29.537897240684302	27.56867689119002	19.284099764583
39	22.44509316719503	27.139255070441497	28.277069894776353	22.138581867587117
40	25.793155362978737	26.838455795052575	27.087438850740725	20.280949991227963
41	21.881336776867936	24.894021550651374	31.289550667694833	21.93509100478586
42	25.161466684618745	26.69769519822764	26.528068479010354	21.612769638143263
43	22.5137394582553	26.808161666605468	28.12060123134922	22.557497643790008
44	23.260280623589843	26.074268554898673	26.701265213364504	23.96418560814698
45	25.331297404700997	26.238897252924353	26.570194657625347	21.859610684749303
46	20.545233111788395	27.39395015034864	29.414068715651354	22.646748022211614
47	22.20763616037732	27.525632708682686	27.455048409405258	22.81168272153474
48	21.775868329681717	25.13402856828113	31.141242038866245	21.948861063170906
49	22.65878407324447	25.688298918387414	28.234433713998946	23.418483294369167
50	20.915086679967523	25.035190149206233	27.905278318380073	26.144444852446174
51	20.89825660860802	23.571891944821846	30.787096537289315	24.742754909280816
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	914.0
1	746.0
2	578.0
3	455.0
4	332.0
5	305.5
6	279.0
7	319.0
8	359.0
9	427.0
10	495.0
11	612.0
12	729.0
13	901.5
14	1074.0
15	972.5
16	871.0
17	1216.0
18	1561.0
19	1840.0
20	2119.0
21	2289.5
22	2460.0
23	3442.5
24	4425.0
25	5457.5
26	8302.0
27	10114.0
28	11701.0
29	13288.0
30	16056.5
31	18825.0
32	22254.5
33	25684.0
34	28346.0
35	31008.0
36	35547.0
37	40086.0
38	43507.0
39	46928.0
40	50787.0
41	54646.0
42	68020.0
43	81394.0
44	86665.5
45	91937.0
46	96859.5
47	101782.0
48	96211.0
49	90640.0
50	84332.0
51	78024.0
52	71204.0
53	64384.0
54	57611.0
55	50838.0
56	44836.5
57	38835.0
58	36266.0
59	33697.0
60	30754.5
61	27812.0
62	24008.0
63	20204.0
64	17032.0
65	13860.0
66	10843.5
67	7827.0
68	6246.0
69	4665.0
70	3767.0
71	2869.0
72	2880.5
73	2892.0
74	2562.0
75	1987.5
76	1743.0
77	1222.5
78	702.0
79	532.0
80	362.0
81	247.0
82	132.0
83	169.0
84	206.0
85	131.0
86	56.0
87	35.0
88	14.0
89	12.0
90	10.0
91	7.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	980388.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.865937370680005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.32502681448612	20.432706049159023
2	10.609291165980379	5.912756863570357
3	3.869758235665762	3.2350332190420583
4	1.9273504256094138	2.1482970500554153
5	1.1783767609345555	1.6418286509633544
6	0.8512521307121172	1.4232563136649061
7	0.6276331438770978	1.224271011532954
8	0.5107308834534829	1.138559584926946
9	0.4267807905236157	1.070338210176634
>10	5.636606970321907	36.7248345825468
>50	0.936712341531562	17.362433134876483
>100	0.0934871999034346	4.038328362377863
>500	0.004048658263534515	0.7452251201563797
>1k	0.0014722393685580055	0.5482009726446256
>5k	0.0014722393685580055	2.3539308743062306
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	6438	0.6566787843180455	TruSeq Adapter, Index 21 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	6327	0.645356736312562	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC	5163	0.5266282329037075	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGT	5023	0.5123481723562507	No Hit
AATGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT	1527	0.1557546603997601	No Hit
AACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	1325	0.1351505730384297	TruSeq Adapter, Index 15 (95% over 21bp)
AATGATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT	1283	0.1308665548741927	No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1210	0.1234205233030188	TruSeq Adapter, Index 15 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0400086496366745E-4	0.0	0.0	0.7517431873911146	0.0
2	2.0400086496366745E-4	0.0	0.0	1.0530524649424513	0.0
3	2.0400086496366745E-4	0.0	0.0	1.4854322982329444	0.0
4	2.0400086496366745E-4	0.0	0.0	3.3586702407618207	0.0
5	2.0400086496366745E-4	0.0	0.0	3.643761449548546	0.0
6	2.0400086496366745E-4	0.0	0.0	5.0389233650350675	0.0
7	2.0400086496366745E-4	0.0	0.0	6.07810377115999	0.0
8	2.0400086496366745E-4	0.0	0.0	6.977747585649763	0.0
9	2.0400086496366745E-4	0.0	0.0	8.636580619101824	0.0
10	2.0400086496366745E-4	0.0	0.0	10.401902104064922	0.0
11	2.0400086496366745E-4	0.0	0.0	12.367858439719784	0.0
12	2.0400086496366745E-4	0.0	0.0	13.098079535857233	0.0
13	2.0400086496366745E-4	0.0	0.0	13.45232703786664	0.0
14	2.0400086496366745E-4	0.0	0.0	13.862062775146166	0.0
15	2.0400086496366745E-4	0.0	0.0	14.169492078646414	0.0
16	2.0400086496366745E-4	0.0	0.0	14.762624593528276	0.0
17	2.0400086496366745E-4	0.0	0.0	15.551495938342779	0.0
18	2.0400086496366745E-4	0.0	0.0	16.49673394615193	0.0
19	2.0400086496366745E-4	0.0	0.0	16.96603793600085	0.0
20	3.0600129744550114E-4	0.0	0.0	17.442583956555975	0.0
21	3.0600129744550114E-4	0.0	0.0	17.98461425476444	0.0
22	3.0600129744550114E-4	0.0	0.0	18.613038919285017	0.0
23	3.0600129744550114E-4	0.0	0.0	19.184649342913215	0.0
24	3.0600129744550114E-4	0.0	0.0	19.618457182258453	0.0
25	3.0600129744550114E-4	0.0	0.0	19.98759674741021	0.0
26	3.0600129744550114E-4	0.0	0.0	20.365814351052848	0.0
27	3.0600129744550114E-4	0.0	0.0	20.775958090062304	0.0
28	3.0600129744550114E-4	0.0	0.0	21.1785537970681	0.0
29	3.0600129744550114E-4	0.0	0.0	21.606751612626837	0.0
30	3.0600129744550114E-4	0.0	0.0	22.203658143510527	0.0
31	3.0600129744550114E-4	0.0	0.0	22.627367940040067	0.0
32	3.0600129744550114E-4	0.0	0.0	23.060155775060487	0.0
33	3.0600129744550114E-4	0.0	0.0	23.47254352358454	0.0
34	3.0600129744550114E-4	0.0	0.0	23.86289917869252	0.0
35	3.0600129744550114E-4	0.0	0.0	24.312109083342513	0.0
36	3.0600129744550114E-4	0.0	0.0	24.726434840083723	0.0
37	3.0600129744550114E-4	0.0	0.0	25.143616608934423	0.0
38	3.0600129744550114E-4	0.0	0.0	25.566102400274175	0.0
39	3.0600129744550114E-4	0.0	0.0	25.99277020934569	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCA	30	2.165174E-6	45.000004	41
CGGGTCG	30	2.165174E-6	45.000004	6
TTTCGCG	30	2.165174E-6	45.000004	1
TAACCCG	30	2.165174E-6	45.000004	1
CATCGCG	35	1.2116834E-7	45.000004	1
CAATGCG	35	1.2116834E-7	45.000004	1
TATAACG	30	2.165174E-6	45.000004	1
TTCGACG	30	2.165174E-6	45.000004	1
AGATCGT	25	3.8903854E-5	45.0	23
GTCGACA	25	3.8903854E-5	45.0	24
CCGATAG	20	7.032778E-4	45.0	1
CCTAGCG	45	3.8380676E-10	45.0	1
CCGGCTA	20	7.032778E-4	45.0	33
GACGTAA	25	3.8903854E-5	45.0	22
GAGTACG	40	6.8139343E-9	45.0	1
CCGGACG	50	2.1827873E-11	45.0	1
CCGTATC	25	3.8903854E-5	45.0	43
GCCGATT	20	7.032778E-4	45.0	14
CGCTATG	20	7.032778E-4	45.0	1
CTACGCG	55	1.8189894E-12	45.0	1
>>END_MODULE