##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553476_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	487753
Sequences flagged as poor quality	0
Sequence length	51
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00831978480912	33.0	31.0	34.0	30.0	34.0
2	32.208402613617956	34.0	31.0	34.0	30.0	34.0
3	32.3355386845391	34.0	31.0	34.0	30.0	34.0
4	35.94368461085836	37.0	35.0	37.0	35.0	37.0
5	35.74941620041292	37.0	35.0	37.0	35.0	37.0
6	35.7403911405978	37.0	35.0	37.0	33.0	37.0
7	35.7339616568222	37.0	35.0	37.0	35.0	37.0
8	35.91594106033177	37.0	35.0	37.0	35.0	37.0
9	37.53161948773252	39.0	37.0	39.0	35.0	39.0
10	37.14115136144729	39.0	37.0	39.0	34.0	39.0
11	37.10440940394011	39.0	37.0	39.0	33.0	39.0
12	36.9781467259043	39.0	37.0	39.0	33.0	39.0
13	36.93768362265327	39.0	37.0	39.0	33.0	39.0
14	38.12585673486375	40.0	37.0	41.0	33.0	41.0
15	38.11543752678097	40.0	37.0	41.0	33.0	41.0
16	38.04386441498053	40.0	37.0	41.0	33.0	41.0
17	38.04996791408767	40.0	37.0	41.0	33.0	41.0
18	38.00174678577066	40.0	37.0	41.0	33.0	41.0
19	37.98844907155876	40.0	37.0	41.0	33.0	41.0
20	37.92984358886567	40.0	37.0	41.0	33.0	41.0
21	37.871639948908566	40.0	37.0	41.0	33.0	41.0
22	37.85858005998938	40.0	37.0	41.0	33.0	41.0
23	37.7494408030294	40.0	36.0	41.0	33.0	41.0
24	37.655745838569935	40.0	36.0	41.0	33.0	41.0
25	37.63844814896064	40.0	36.0	41.0	33.0	41.0
26	37.58710043813159	39.0	36.0	41.0	33.0	41.0
27	37.53326171238311	39.0	36.0	41.0	33.0	41.0
28	37.44352571896021	39.0	36.0	41.0	32.0	41.0
29	37.3088079417246	39.0	36.0	41.0	32.0	41.0
30	37.2915246036416	39.0	36.0	41.0	32.0	41.0
31	37.20051132437935	39.0	35.0	41.0	32.0	41.0
32	37.18077797573772	39.0	35.0	41.0	32.0	41.0
33	37.085610954725034	39.0	35.0	41.0	31.0	41.0
34	37.00112967014042	39.0	35.0	41.0	31.0	41.0
35	36.89014111650774	39.0	35.0	41.0	31.0	41.0
36	36.81529380649632	39.0	35.0	41.0	31.0	41.0
37	36.6700543102759	39.0	35.0	40.0	31.0	41.0
38	36.63299046853633	39.0	35.0	40.0	31.0	41.0
39	36.542092001484356	39.0	35.0	40.0	30.0	41.0
40	36.43393274874783	39.0	35.0	40.0	30.0	41.0
41	36.38570546977671	38.0	35.0	40.0	30.0	41.0
42	36.291577909310654	38.0	35.0	40.0	30.0	41.0
43	36.199525169501776	38.0	35.0	40.0	30.0	41.0
44	36.04127703981318	38.0	35.0	40.0	30.0	41.0
45	35.847156245066664	38.0	35.0	40.0	29.0	41.0
46	35.73092118346786	38.0	34.0	40.0	29.0	41.0
47	35.60378306232868	38.0	34.0	40.0	29.0	41.0
48	35.51279233546488	38.0	34.0	40.0	28.0	41.0
49	35.34787484648992	37.0	34.0	40.0	28.0	41.0
50	35.1739999548952	37.0	34.0	40.0	27.0	41.0
51	33.898245628422586	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	0.0
11	6.0
12	7.0
13	9.0
14	14.0
15	25.0
16	52.0
17	92.0
18	161.0
19	332.0
20	544.0
21	790.0
22	1157.0
23	1546.0
24	1928.0
25	2392.0
26	3090.0
27	3912.0
28	5220.0
29	6548.0
30	8573.0
31	11308.0
32	14903.0
33	20125.0
34	32097.0
35	38943.0
36	45674.0
37	66264.0
38	101498.0
39	120514.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.592691382728553	31.642655196380137	20.263740048754187	26.500913372137124
2	23.390322560804343	31.452394962204234	19.428891262585775	25.728391214405654
3	21.452456468745453	29.907760690349416	20.99689802010444	27.64288482080069
4	21.61483373756799	31.085405932920967	19.174664225540386	28.12509610397066
5	18.536226327669947	33.389645988850916	17.508862067480877	30.56526561599826
6	21.095308486057494	35.7752797009962	20.061178506334148	23.068233306612157
7	84.72259524800462	5.118779382187295	5.4025295590186015	4.7560958107894775
8	86.53642314860186	3.985623871098691	4.405508525831722	5.072444454467733
9	79.46645125709118	7.429580135847447	8.027833760120389	5.076134846940972
10	38.36080967210863	31.20759892814601	13.328672504320835	17.10291889542453
11	27.923354648766896	28.5146375317015	23.71364194582094	19.84836587371067
12	28.785676356680533	23.409184566778677	28.05600375599945	19.74913532054134
13	27.382917173241374	23.629788027956774	26.563650044182197	22.423644754619655
14	22.987864759417164	25.482775093131156	28.877116081295245	22.652244066156435
15	22.12185265903029	27.386607565714616	27.679583723729017	22.81195605152608
16	23.912718117571803	25.82270124427733	27.150012403819147	23.11456823433172
17	22.53230631077615	26.54929851789738	27.611721506582228	23.306673664744245
18	24.362330933894818	24.745311663895457	26.26083283957249	24.631524562637235
19	24.058078576656627	28.731140556798216	25.93771847635996	21.273062390185196
20	26.035513876900808	27.00506198834246	25.14940963971518	21.810014495041546
21	25.74930343842068	27.28799207795749	24.81850444794804	22.144200035673794
22	24.121225292309838	25.743562827906747	25.00917472573208	25.126037154051335
23	24.475092926132692	27.15677812335342	23.575867293486663	24.792261657027225
24	23.22856035739401	26.886149341982517	25.002203984393738	24.88308631622973
25	23.69888037592798	26.262062970396904	24.23378226274364	25.805274390931476
26	25.707068946782492	26.147660803726474	24.34039360085945	23.80487664863158
27	23.29929287979777	25.643102143913005	25.354841487392182	25.702763488897045
28	23.78006901033925	25.847303860765592	26.707780372442606	23.664846756452548
29	25.47293404653585	27.29598792831618	23.497343942528286	23.733734082619687
30	26.674566840183456	24.33895844823097	25.64884275442693	23.337631957158646
31	26.59440331479253	25.81880583000002	22.669670919502288	24.917119935705163
32	27.766717990458282	24.247313701812185	24.62434879949483	23.361619508234703
33	26.57226095995309	24.59954116120249	25.157200468269803	23.670997410574614
34	24.93229154920626	24.532909074880113	26.253862098234148	24.28093727767948
35	26.329925187543697	23.88257991237368	27.12581983093902	22.6616750691436
36	27.27487068249708	26.086769327918024	24.771964498424406	21.866395491160485
37	26.603219252367488	26.70450002357751	24.579859068011885	22.11242165604312
38	27.075589488942146	24.873655313242562	25.612553895106743	22.438201302708542
39	25.74376784971082	22.776692301226237	25.968061703362157	25.511478145700796
40	26.544583016403795	23.341732393240022	26.331565361976246	23.78211922837994
41	22.672541224759254	22.881663464909494	28.972451220187267	25.473344090143986
42	25.005074289650704	23.970124222711085	26.458268836890802	24.56653265074741
43	24.137011971223142	23.12399923731889	27.86123304213403	24.877755749323942
44	25.84238333746794	22.032668174260333	25.751763700069503	26.373184788202224
45	26.143560367645097	22.938044461028433	26.82259258272117	24.0958025886053
46	24.30492482875554	23.209903373223742	27.727558825881133	24.757612972139587
47	23.85059651093894	25.845048620920835	25.952890089861057	24.35146477827917
48	23.580377773176178	23.75116093596554	28.5119722482486	24.15648904260968
49	24.37709250378778	23.763052200601535	27.27241042084826	24.587444874762433
50	23.43378718326694	22.954856248962077	27.00260172669363	26.60875484107735
51	23.549009437153643	22.34307118562059	27.99121686591369	26.116702511312077
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	816.0
1	719.0
2	622.0
3	441.0
4	260.0
5	250.5
6	241.0
7	286.0
8	331.0
9	393.0
10	455.0
11	471.5
12	488.0
13	540.5
14	593.0
15	604.5
16	616.0
17	635.5
18	655.0
19	714.0
20	773.0
21	863.0
22	953.0
23	1380.5
24	1808.0
25	2015.0
26	2652.0
27	3082.0
28	3806.5
29	4531.0
30	5297.5
31	6064.0
32	7522.0
33	8980.0
34	9720.5
35	10461.0
36	11790.0
37	13119.0
38	13670.0
39	14221.0
40	15413.5
41	16606.0
42	18489.5
43	20373.0
44	22668.5
45	24964.0
46	28566.5
47	32169.0
48	35301.5
49	38434.0
50	42428.0
51	46422.0
52	44262.0
53	42102.0
54	38189.5
55	34277.0
56	32047.5
57	29818.0
58	28343.0
59	26868.0
60	25797.0
61	24726.0
62	23234.5
63	21743.0
64	19204.0
65	16665.0
66	14724.0
67	12783.0
68	10871.5
69	8960.0
70	7593.0
71	6226.0
72	5640.5
73	5055.0
74	4178.5
75	2639.0
76	1976.0
77	1601.0
78	1226.0
79	929.5
80	633.0
81	585.0
82	537.0
83	455.5
84	374.0
85	245.0
86	116.0
87	82.5
88	49.0
89	40.0
90	31.0
91	23.0
92	15.0
93	10.5
94	6.0
95	5.0
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	487753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.981453774469173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.44887099082442	21.866179669564662
2	9.418208337899802	5.459067391663266
3	3.2555648666597365	2.830530080786552
4	1.6018987221047079	1.8570141506423534
5	0.9652681299036749	1.398743684338583
6	0.7308830446067008	1.2709231903087437
7	0.5714624678917071	1.1593269167933309
8	0.46330035463101155	1.074169424914707
9	0.41284189211298883	1.0768282391193278
>10	6.34725218124898	45.34589092788865
>50	0.7319632735117331	13.037994029700725
>100	0.04893940491502867	2.47633086453624
>500	0.0028370669515958647	0.6534619256545785
>1k	7.092667378989662E-4	0.4935395040882803
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT	2401	0.49225735156933936	No Hit
AATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT	880	0.1804191875805992	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCC	861	0.1765237733032908	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGT	810	0.1660676612957788	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	628	0.1287536929552458	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5617597431486838	0.0
2	0.0	0.0	0.0	0.7749824193803011	0.0
3	0.0	0.0	0.0	1.033514914311137	0.0
4	0.0	0.0	0.0	2.1851223877659387	0.0
5	0.0	0.0	0.0	2.3755876437459125	0.0
6	0.0	0.0	0.0	3.5671743689941424	0.0
7	0.0	0.0	0.0	4.393617261195728	0.0
8	0.0	0.0	0.0	5.164089200886514	0.0
9	0.0	0.0	0.0	6.20149952947496	0.0
10	0.0	0.0	0.0	7.727066773551368	0.0
11	0.0	0.0	0.0	9.19399778166408	0.0
12	0.0	0.0	0.0	9.851502707312923	0.0
13	0.0	0.0	0.0	10.149604410429049	0.0
14	0.0	0.0	0.0	10.49199082322405	0.0
15	0.0	0.0	0.0	10.772255629386185	0.0
16	0.0	0.0	0.0	11.297111447802473	0.0
17	0.0	0.0	0.0	11.916892361502645	0.0
18	0.0	0.0	0.0	12.69864050041722	0.0
19	0.0	0.0	0.0	13.045127349293598	0.0
20	0.0	0.0	0.0	13.452300652174358	0.0
21	0.0	0.0	0.0	13.878540982833524	0.0
22	0.0	0.0	0.0	14.323233275858888	0.0
23	0.0	0.0	0.0	14.786787574858586	0.0
24	0.0	0.0	0.0	15.13245433651869	0.0
25	0.0	0.0	0.0	15.455568699731216	0.0
26	0.0	0.0	0.0	15.750800097590378	0.0
27	0.0	0.0	0.0	16.086215769047037	0.0
28	0.0	0.0	0.0	16.39210830071778	0.0
29	0.0	0.0	0.0	16.723218514288995	0.0
30	0.0	0.0	0.0	17.191693336586347	0.0
31	0.0	0.0	0.0	17.50660682763612	0.0
32	0.0	0.0	0.0	17.831771408889335	0.0
33	0.0	0.0	0.0	18.15181044504083	0.0
34	0.0	0.0	0.0	18.461598390988883	0.0
35	0.0	0.0	0.0	18.801934585743194	0.0
36	0.0	0.0	0.0	19.1541620451335	0.0
37	0.0	0.0	0.0	19.47543121210941	0.0
38	0.0	0.0	0.0	19.80264601140331	0.0
39	0.0	0.0	0.0	20.173940498572023	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGAC	35	1.21001E-7	45.000004	21
AAACGCG	35	1.21001E-7	45.000004	1
CTAACGG	115	0.0	45.000004	2
TCGACGG	35	1.21001E-7	45.000004	2
TCACGAC	25	3.887493E-5	45.0	25
GGTACTC	20	7.02929E-4	45.0	8
TTAATCG	90	0.0	45.0	20
CTATACG	20	7.02929E-4	45.0	1
ATTACGG	20	7.02929E-4	45.0	2
TAAACGC	25	3.887493E-5	45.0	40
GCGTTAC	25	3.887493E-5	45.0	17
ATCGTTA	20	7.02929E-4	45.0	34
CGTTACG	20	7.02929E-4	45.0	1
GCATTAC	20	7.02929E-4	45.0	14
ATATACG	25	3.887493E-5	45.0	1
TACGACG	20	7.02929E-4	45.0	1
TATTCGC	25	3.887493E-5	45.0	44
ACGTGCA	25	3.887493E-5	45.0	34
TCATGCG	20	7.02929E-4	45.0	1
ATCCACG	20	7.02929E-4	45.0	1
>>END_MODULE