FastQCFastQC Report
Sat 18 Jun 2016
SRR3553473_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553473_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885032
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT59440.6716141337262381No Hit
AATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT17560.1984109049164324No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC13070.14767827603973643No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCC12940.14620940259787218No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGT11980.13536233718102847No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10420.11773585587865751No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCG207.032405E-445.0000041
GTCGAGA207.032405E-445.0000048
CGACGAA207.032405E-445.0000047
GACCGTA207.032405E-445.00000422
TTGTCGA207.032405E-445.00000429
CGACACG207.032405E-445.0000041
CCGCTCA207.032405E-445.00000414
CCCGTAA207.032405E-445.00000438
TCGTCGA207.032405E-445.00000441
AGCGACG351.2115015E-745.0000041
TCCCGTA207.032405E-445.00000437
CGTGTAA207.032405E-445.00000416
TATAGCG351.2115015E-745.0000041
CGTCGGT207.032405E-445.00000418
CGTCGAA207.032405E-445.00000442
AACTCGC207.032405E-445.00000441
CGGTCGA207.032405E-445.0000046
ACCAACG406.8121153E-945.0000041
GCGTGAT207.032405E-445.0000049
CCGCGAA207.032405E-445.0000043