FastQCFastQC Report
Sat 18 Jun 2016
SRR3553472_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553472_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977889
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT42730.43696165924762426Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC10600.10839676077755248No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG253.8903763E-545.0000041
CCGTACA253.8903763E-545.00000421
CCGAACC253.8903763E-545.00000424
TAAGACG253.8903763E-545.0000041
CGAAACG551.8189894E-1245.01
GTCACGA207.0327695E-445.025
GTATCGA207.0327695E-445.023
GTATACG207.0327695E-445.01
CAGTACG551.8189894E-1245.01
GCGTAAG453.8380676E-1045.01
ACGAACG453.8380676E-1045.01
CGATAAG302.1651686E-644.9999961
ATTACCG750.042.01
TATAGCG650.041.538461
ACTACGG1900.041.447372
ATCACCG556.002665E-1140.9090921
AAACGCG556.002665E-1140.9090921
TACTACG950.040.2631571
ATAAGCG451.927765E-840.01
GTGGCGA1700.039.70588328
Produced by FastQC (version 0.11.5)