FastQCFastQC Report
Sat 18 Jun 2016
SRR3553471_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553471_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987140
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT44240.44816338108069775Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC9890.10018842312134044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACCG950.045.0000041
AACGTTC207.032803E-445.039
CTATGCG253.890404E-545.01
GTCGACG207.032803E-445.01
TCGATCT207.032803E-445.018
ATCGTTA207.032803E-445.045
CGTTATG406.8139343E-945.01
TATAGCG453.8380676E-1045.01
ATCGATC207.032803E-445.017
TTACGCG302.1651904E-644.9999961
ATACCCG302.1651904E-644.9999961
CAGTACG850.042.3529431
CATGACG750.042.01
AAACGCG603.6379788E-1241.2499961
ATTAGCG501.0804797E-940.51
TACGCGG900.040.02
AATTACG451.927765E-840.01
TCGTTCG850.039.70588345
TTACCGG1700.039.7058832
TATCACG403.4575532E-739.3751
Produced by FastQC (version 0.11.5)