##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553471_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	987140
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.941919079360577	33.0	31.0	34.0	30.0	34.0
2	32.135714285714286	34.0	31.0	34.0	30.0	34.0
3	32.287151771785155	34.0	31.0	34.0	30.0	34.0
4	35.86900844864964	37.0	35.0	37.0	35.0	37.0
5	35.69769029722228	37.0	35.0	37.0	33.0	37.0
6	35.70491419656786	37.0	35.0	37.0	33.0	37.0
7	35.818264886439614	37.0	35.0	37.0	35.0	37.0
8	35.95877889661041	37.0	35.0	37.0	35.0	37.0
9	37.567006706242275	39.0	37.0	39.0	35.0	39.0
10	37.101081913406404	39.0	37.0	39.0	33.0	39.0
11	37.084641489555686	39.0	37.0	39.0	33.0	39.0
12	37.044291589845415	39.0	37.0	39.0	33.0	39.0
13	37.021124663168344	39.0	37.0	39.0	33.0	39.0
14	38.24122515549973	40.0	38.0	41.0	33.0	41.0
15	38.21894665397006	40.0	37.0	41.0	33.0	41.0
16	38.186966387746416	40.0	37.0	41.0	33.0	41.0
17	38.151414186437584	40.0	37.0	41.0	33.0	41.0
18	38.100796239641795	40.0	37.0	41.0	33.0	41.0
19	38.11406386125575	40.0	37.0	41.0	33.0	41.0
20	38.04343558157911	40.0	37.0	41.0	33.0	41.0
21	38.02339789695484	40.0	37.0	41.0	33.0	41.0
22	37.98829547987114	40.0	37.0	41.0	33.0	41.0
23	37.88520371983711	40.0	37.0	41.0	33.0	41.0
24	37.79500982636708	40.0	37.0	41.0	33.0	41.0
25	37.7802763539113	40.0	37.0	41.0	33.0	41.0
26	37.70100391028628	40.0	37.0	41.0	33.0	41.0
27	37.6635512693235	40.0	37.0	41.0	33.0	41.0
28	37.5967431164779	40.0	36.0	41.0	33.0	41.0
29	37.47115100188423	39.0	36.0	41.0	32.0	41.0
30	37.46770468221326	39.0	36.0	41.0	32.0	41.0
31	37.42355491622262	39.0	36.0	41.0	32.0	41.0
32	37.39405251534737	39.0	36.0	41.0	32.0	41.0
33	37.31611625503981	39.0	36.0	41.0	32.0	41.0
34	37.22208501327066	39.0	36.0	41.0	31.0	41.0
35	37.148883643657435	39.0	36.0	41.0	31.0	41.0
36	37.07492047733857	39.0	35.0	41.0	31.0	41.0
37	36.979863038677394	39.0	35.0	41.0	31.0	41.0
38	36.88457868184857	39.0	35.0	41.0	31.0	41.0
39	36.81123143627044	39.0	35.0	41.0	31.0	41.0
40	36.725141317341006	39.0	35.0	40.0	31.0	41.0
41	36.679173166926674	39.0	35.0	40.0	31.0	41.0
42	36.55925198046883	39.0	35.0	40.0	30.0	41.0
43	36.49263832890978	39.0	35.0	40.0	30.0	41.0
44	36.43166014952286	39.0	35.0	40.0	30.0	41.0
45	36.27491034706323	38.0	35.0	40.0	30.0	41.0
46	36.15730494154831	38.0	35.0	40.0	30.0	41.0
47	36.0495958020139	38.0	35.0	40.0	30.0	41.0
48	35.98988492007213	38.0	35.0	40.0	29.0	41.0
49	35.83235407338371	38.0	35.0	40.0	29.0	41.0
50	35.65278177360861	38.0	34.0	40.0	28.0	41.0
51	34.451187268269955	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	4.0
10	9.0
11	8.0
12	11.0
13	13.0
14	15.0
15	39.0
16	65.0
17	104.0
18	230.0
19	444.0
20	709.0
21	1205.0
22	1771.0
23	2395.0
24	3300.0
25	4413.0
26	5680.0
27	7625.0
28	9659.0
29	13036.0
30	16745.0
31	21966.0
32	28831.0
33	38938.0
34	58910.0
35	72804.0
36	90291.0
37	132919.0
38	209279.0
39	265645.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.549304050084082	32.00153980185181	21.041696213303076	27.407459934761025
2	22.476447109832446	29.924732054217234	20.8287578256377	26.770063010312622
3	21.12172538849606	30.52363393237028	20.35719350851956	27.997447170614098
4	20.522418299329377	30.78783151326053	19.818971979658407	28.870778207751684
5	19.06477298052961	33.34602994509391	17.82624551735316	29.76295155702332
6	20.92560325789655	36.69469376177644	18.850720262576736	23.528982717750267
7	86.496849484369	4.022428429604717	4.725570841015459	4.755151245010839
8	87.95449480316874	3.859229693863079	4.026885750754706	4.159389752213465
9	80.04761229410215	7.635796340944546	7.614016248961647	4.702575115991653
10	34.43108373685597	36.5772838705756	13.22122495289422	15.77040743967421
11	24.507668618433048	27.48303178880402	29.412241424721923	18.59705816804101
12	26.56897704479608	22.961079482140327	30.187004882792714	20.28293859027088
13	25.20564458941994	23.476406588731084	28.35028466073708	22.9676641611119
14	21.229207609862836	25.551796097817935	29.590939481735113	23.628056810584113
15	20.38343092165245	28.544076828008187	29.151488137447572	21.921004112891787
16	22.4025974025974	26.483578823672428	28.207751686690845	22.906072087039327
17	21.808152845594346	26.782827157242135	29.04755151245011	22.361468484713416
18	22.431873898332558	25.54460360232591	28.22183276941467	23.80168972992686
19	22.696780598496666	29.04319549405353	26.79022225824098	21.469801649208826
20	24.34740766254027	27.379196466559964	26.7457503494945	21.527645521405272
21	23.93176246530381	27.70255485544097	25.777093421399194	22.58858925785603
22	22.79494296654983	25.76564621026399	26.393014162124928	25.046396661061248
23	23.110095832404724	27.42073059545758	25.53508114350548	23.934092428632212
24	21.25706586705027	27.00528800372794	27.45861782523249	24.279028303989303
25	22.69293109386713	27.3773730169986	25.88974208318982	24.039953805944446
26	23.196000567295417	27.786433535263487	25.622505419697305	23.395060477743783
27	21.542131815142735	26.46139352067589	26.106428672731326	25.89004599145005
28	22.00356585691999	27.33482586056689	27.282958850821565	23.378649431691553
29	22.921064894543832	27.42073059545758	24.24063456044735	25.41756994955123
30	22.972121482261887	26.261016674433215	26.326863464148953	24.439998379155945
31	23.138055392345564	26.955244443543975	23.667058370646515	26.239641793463946
32	24.203152541686084	25.821565330145674	24.621735518771402	25.353546609396844
33	22.369572704986123	25.697570759973253	26.115951131551757	25.816905403488867
34	21.7274145511275	25.999756873391817	26.58093076969832	25.69189780578236
35	22.223595437323986	25.170391231233662	27.15947079441619	25.446542537026158
36	22.361569787466824	27.73760560812043	25.386267398747897	24.51455720566485
37	23.321717284275785	27.376967805984965	24.515671535952347	24.7856433737869
38	23.483193873209473	26.304880766659238	26.543752659197278	23.66817270093401
39	23.14372834653646	25.782259861823043	26.46098830966226	24.613023481978242
40	24.883704439086653	24.948335595761492	27.274044208521588	22.893915756630264
41	23.414915817411917	24.214903661081507	28.74425106874405	23.625929452762527
42	24.093137751484086	25.287395911420873	26.09721012217112	24.52225621492392
43	21.34236278542051	25.0045586239034	27.663350689871752	25.989727900804343
44	23.410661101768746	23.9339911258788	26.05638511254736	26.598962659805093
45	23.436088092874364	24.45468727840023	27.645825313532026	24.46339931519339
46	21.355836051623882	25.36367688473773	28.62380209494094	24.656684968697448
47	22.30524545657151	25.372388921530884	27.490528192556273	24.83183742934133
48	22.67378487347286	24.268999331401826	28.657333306319266	24.399882488806046
49	22.961687298660777	24.5518366189193	27.200599712300182	25.28587637011974
50	21.984419636525722	23.559373543772917	27.295013878477214	27.161192941224144
51	22.015316976315415	23.533946552667302	28.573150718236523	25.877585752780764
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	963.0
1	851.5
2	740.0
3	539.5
4	339.0
5	358.0
6	377.0
7	406.0
8	435.0
9	509.5
10	584.0
11	674.0
12	764.0
13	888.0
14	1012.0
15	1088.0
16	1164.0
17	1173.5
18	1183.0
19	1473.0
20	1763.0
21	2238.0
22	2713.0
23	3421.5
24	4130.0
25	4603.0
26	6399.5
27	7723.0
28	9779.0
29	11835.0
30	14257.5
31	16680.0
32	19960.0
33	23240.0
34	25078.5
35	26917.0
36	30526.5
37	34136.0
38	35744.5
39	37353.0
40	40232.0
41	43111.0
42	45496.5
43	47882.0
44	52012.0
45	56142.0
46	63148.5
47	70155.0
48	76065.5
49	81976.0
50	89243.5
51	96511.0
52	90419.5
53	84328.0
54	76620.5
55	68913.0
56	61150.0
57	53387.0
58	49615.0
59	45843.0
60	42487.0
61	39131.0
62	36746.5
63	34362.0
64	29411.0
65	24460.0
66	21243.0
67	18026.0
68	15690.0
69	13354.0
70	11715.5
71	10077.0
72	8384.0
73	6691.0
74	6345.5
75	4626.0
76	3252.0
77	2566.0
78	1880.0
79	1517.5
80	1155.0
81	839.5
82	524.0
83	534.0
84	544.0
85	349.5
86	155.0
87	100.5
88	46.0
89	53.0
90	60.0
91	47.5
92	35.0
93	21.5
94	8.0
95	5.0
96	2.0
97	1.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	987140.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.894563658625355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.12306132107386	21.41753513913959
2	9.791971190323641	5.658694698044639
3	3.5555538902117547	3.082085346671897
4	1.7724972558009546	2.0486213916991773
5	1.1435051756595005	1.6520541546030503
6	0.8753559284209393	1.517581655862836
7	0.6398673153623583	1.2942080813767958
8	0.5153607043041782	1.1912898142136858
9	0.4832076327597656	1.2565866334599636
>10	6.172869251437362	41.10936907097127
>50	0.8709475403882578	16.221332088317673
>100	0.05333051856250558	2.677191971868688
>500	0.002119093452814633	0.4219786842904831
>1k	3.5318224213577216E-4	0.4514712694803137
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	4424	0.44816338108069775	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	989	0.10018842312134044	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0130275340883765E-4	0.0	0.0	0.5094515468930445	0.0
2	1.0130275340883765E-4	0.0	0.0	0.7553133294162936	0.0
3	1.0130275340883765E-4	0.0	0.0	0.9790911116964159	0.0
4	1.0130275340883765E-4	0.0	0.0	1.937516461697429	0.0
5	1.0130275340883765E-4	0.0	0.0	2.1131754361083535	0.0
6	1.0130275340883765E-4	0.0	0.0	3.319184715440566	0.0
7	1.0130275340883765E-4	0.0	0.0	4.240938468707579	0.0
8	1.0130275340883765E-4	0.0	0.0	5.154891909962113	0.0
9	1.0130275340883765E-4	0.0	0.0	6.250582490832101	0.0
10	1.0130275340883765E-4	0.0	0.0	7.954190894908524	0.0
11	1.0130275340883765E-4	0.0	0.0	9.308203496971048	0.0
12	1.0130275340883765E-4	0.0	0.0	9.997264825657961	0.0
13	1.0130275340883765E-4	0.0	0.0	10.3198127925117	0.0
14	1.0130275340883765E-4	0.0	0.0	10.6579613833904	0.0
15	1.0130275340883765E-4	0.0	0.0	10.926211074417003	0.0
16	1.0130275340883765E-4	0.0	0.0	11.454099722430456	0.0
17	1.0130275340883765E-4	0.0	0.0	12.125331766517414	0.0
18	1.0130275340883765E-4	0.0	0.0	12.889458435480277	0.0
19	1.0130275340883765E-4	0.0	0.0	13.281094880158843	0.0
20	1.0130275340883765E-4	0.0	0.0	13.71446805924185	0.0
21	1.0130275340883765E-4	0.0	0.0	14.188159734181575	0.0
22	1.0130275340883765E-4	0.0	0.0	14.724253905221143	0.0
23	1.0130275340883765E-4	0.0	0.0	15.2475839293312	0.0
24	1.0130275340883765E-4	0.0	0.0	15.659987438458577	0.0
25	1.0130275340883765E-4	0.0	0.0	16.02508256174403	0.0
26	1.0130275340883765E-4	0.0	0.0	16.358368620459103	0.0
27	1.0130275340883765E-4	0.0	0.0	16.715663431732075	0.0
28	1.0130275340883765E-4	0.0	0.0	17.06617095852665	0.0
29	1.0130275340883765E-4	0.0	0.0	17.431873898332558	0.0
30	1.0130275340883765E-4	0.0	0.0	17.941122839718783	0.0
31	1.0130275340883765E-4	0.0	0.0	18.3207042567417	0.0
32	1.0130275340883765E-4	0.0	0.0	18.70676904998278	0.0
33	1.0130275340883765E-4	0.0	0.0	19.09293514597727	0.0
34	1.0130275340883765E-4	0.0	0.0	19.453775553619547	0.0
35	1.0130275340883765E-4	0.0	0.0	19.824442328342485	0.0
36	1.0130275340883765E-4	0.0	0.0	20.203719837105172	0.0
37	1.0130275340883765E-4	0.0	0.0	20.552201308831574	0.0
38	1.0130275340883765E-4	0.0	0.0	20.93593613874425	0.0
39	1.0130275340883765E-4	0.0	0.0	21.308527665781956	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACCG	95	0.0	45.000004	1
AACGTTC	20	7.032803E-4	45.0	39
CTATGCG	25	3.890404E-5	45.0	1
GTCGACG	20	7.032803E-4	45.0	1
TCGATCT	20	7.032803E-4	45.0	18
ATCGTTA	20	7.032803E-4	45.0	45
CGTTATG	40	6.8139343E-9	45.0	1
TATAGCG	45	3.8380676E-10	45.0	1
ATCGATC	20	7.032803E-4	45.0	17
TTACGCG	30	2.1651904E-6	44.999996	1
ATACCCG	30	2.1651904E-6	44.999996	1
CAGTACG	85	0.0	42.352943	1
CATGACG	75	0.0	42.0	1
AAACGCG	60	3.6379788E-12	41.249996	1
ATTAGCG	50	1.0804797E-9	40.5	1
TACGCGG	90	0.0	40.0	2
AATTACG	45	1.927765E-8	40.0	1
TCGTTCG	85	0.0	39.705883	45
TTACCGG	170	0.0	39.705883	2
TATCACG	40	3.4575532E-7	39.375	1
>>END_MODULE