##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553468_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	769470
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83603129426748	33.0	31.0	34.0	30.0	34.0
2	32.04639817016908	33.0	31.0	34.0	30.0	34.0
3	32.18803852002027	34.0	31.0	34.0	30.0	34.0
4	35.81795261673619	37.0	35.0	37.0	35.0	37.0
5	35.62859370735701	37.0	35.0	37.0	33.0	37.0
6	35.620134638127546	37.0	35.0	37.0	33.0	37.0
7	35.77917917527649	37.0	35.0	37.0	35.0	37.0
8	35.93596891366785	37.0	35.0	37.0	35.0	37.0
9	37.52507700105267	39.0	37.0	39.0	35.0	39.0
10	37.03160097209768	39.0	37.0	39.0	33.0	39.0
11	37.01209403875395	39.0	37.0	39.0	33.0	39.0
12	37.033068215784894	39.0	37.0	39.0	33.0	39.0
13	37.01892991279712	39.0	37.0	39.0	33.0	39.0
14	38.20708929522918	40.0	38.0	41.0	33.0	41.0
15	38.183149440524	40.0	37.0	41.0	33.0	41.0
16	38.10334645925117	40.0	37.0	41.0	33.0	41.0
17	38.1081016803774	40.0	37.0	41.0	33.0	41.0
18	38.078338336777264	40.0	37.0	41.0	33.0	41.0
19	38.09053894238892	40.0	37.0	41.0	33.0	41.0
20	38.01563933616645	40.0	37.0	41.0	33.0	41.0
21	37.9671540151013	40.0	37.0	41.0	33.0	41.0
22	37.98694555993086	40.0	37.0	41.0	33.0	41.0
23	37.87980817965612	40.0	37.0	41.0	33.0	41.0
24	37.77474365472338	40.0	37.0	41.0	33.0	41.0
25	37.775650772609715	40.0	37.0	41.0	33.0	41.0
26	37.71510780147374	40.0	37.0	41.0	33.0	41.0
27	37.702331474911304	40.0	37.0	41.0	33.0	41.0
28	37.64906753999506	40.0	37.0	41.0	33.0	41.0
29	37.51585636866935	40.0	37.0	41.0	32.0	41.0
30	37.479682118861035	39.0	36.0	41.0	32.0	41.0
31	37.38571224349227	39.0	36.0	41.0	32.0	41.0
32	37.370148283883715	39.0	36.0	41.0	32.0	41.0
33	37.2295943961428	39.0	36.0	41.0	31.0	41.0
34	37.25478706122396	39.0	36.0	41.0	31.0	41.0
35	37.08387071620726	39.0	36.0	41.0	31.0	41.0
36	37.0521137926105	39.0	36.0	41.0	31.0	41.0
37	37.005575266092244	39.0	35.0	41.0	31.0	41.0
38	36.98101160539073	39.0	35.0	41.0	31.0	41.0
39	36.84090607820968	39.0	35.0	41.0	30.0	41.0
40	36.72066747241608	39.0	35.0	40.0	30.0	41.0
41	36.70283441849585	39.0	35.0	40.0	30.0	41.0
42	36.675814521683755	39.0	35.0	40.0	30.0	41.0
43	36.65852989720197	39.0	35.0	40.0	30.0	41.0
44	36.6014061626834	39.0	35.0	40.0	30.0	41.0
45	36.41339883296295	39.0	35.0	40.0	30.0	41.0
46	36.24806685120927	38.0	35.0	40.0	30.0	41.0
47	36.150421718845436	38.0	35.0	40.0	29.0	41.0
48	36.13237033282649	38.0	35.0	40.0	29.0	41.0
49	35.974306990525946	38.0	35.0	40.0	29.0	41.0
50	35.8527207038611	38.0	34.0	40.0	28.0	41.0
51	34.56758288172379	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	7.0
12	6.0
13	14.0
14	17.0
15	18.0
16	32.0
17	57.0
18	147.0
19	277.0
20	527.0
21	833.0
22	1271.0
23	1884.0
24	2501.0
25	3242.0
26	4308.0
27	5694.0
28	7596.0
29	10075.0
30	13436.0
31	17551.0
32	23061.0
33	30616.0
34	43628.0
35	56278.0
36	71867.0
37	105615.0
38	165613.0
39	203242.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.48558098431388	32.77957555200332	21.21031359247274	27.524529871210053
2	22.751244363003106	31.134547155834536	21.352489375804126	24.761719105358235
3	20.52386707733895	31.214602258697543	20.627834743394803	27.6336959205687
4	20.080574941193287	31.17418482851833	20.191040586377635	28.554199643910742
5	18.65972682495744	33.477328550820694	18.12858201099458	29.73436261322729
6	20.816666016868755	35.42711216811571	19.734492572809856	24.021729242205677
7	85.87469297048618	3.900346992085461	5.4697389111986165	4.755221126229743
8	87.33738807230951	3.6157355062575536	4.234343119289901	4.812533302143033
9	78.95980350111115	7.9033620544010805	8.54120368565376	4.595630758834003
10	30.542451297646434	42.286119016985715	12.553705797496978	14.617723887870874
11	21.329746448854404	26.749580880346212	34.01938996971941	17.901282701079964
12	24.689591537031983	23.59117314515186	32.36981298816068	19.34942232965548
13	24.555343288237356	24.719222321857902	29.259620258099734	21.465814131805008
14	19.11588495977751	27.738833222867687	31.0589106787789	22.086371138575903
15	17.872951512079744	30.66721249691346	30.519318491949004	20.940517499057794
16	20.52035816860956	28.262050502293786	29.994671657114637	21.222919671982012
17	19.310434454884533	28.29493027668395	30.59560476691749	21.79903050151403
18	20.80639920984574	27.272018402276892	28.740041846985587	23.18154054089178
19	21.176004262674308	30.40768321052153	28.75810622896279	19.65820629784137
20	23.45783461343522	28.677401328186935	28.299738781239036	19.56502527713881
21	21.758223192587106	29.669512781524947	26.872782564622405	21.699481461265545
22	20.60249262479369	26.884348967471116	28.329629485230097	24.1835289225051
23	21.27776261582648	29.483410659284964	25.784241101017585	23.454585623870976
24	18.301298296229874	28.610602102746046	28.70365316386604	24.384446437158044
25	20.142305742913955	29.30432635450375	25.845971902738246	24.70739599984405
26	22.119640791713778	29.610641090620817	26.19868220983274	22.071035907832666
27	19.78192782044784	28.134170273045083	26.680182463253928	25.40371944325315
28	20.854614214979144	28.216044810064073	27.455261413700338	23.47407956125645
29	22.737078768502997	28.67337258112727	25.84519214524283	22.744356505126905
30	22.004106722809205	25.994645665198124	26.375167322962557	25.626080289030114
31	23.95999844048501	28.51352229456639	23.64029786736325	23.88618139758535
32	23.42196576864595	27.25772284819421	25.00487348434637	24.315437898813467
33	21.593434441888572	27.342976334360014	26.293162826360998	24.77042639739041
34	22.363834847362472	26.34540657855407	26.29563205842983	24.99512651565363
35	21.21785124826179	26.08639713049242	28.161201866219603	24.534549755026188
36	22.627782759561775	28.954735077390932	26.326302519916307	22.091179643130985
37	23.760250562075193	27.368318452961127	26.087436807152976	22.7839941778107
38	24.406799485360054	25.445176550092917	28.78968640752726	21.358337557019766
39	23.569599854445265	24.219007888546663	29.044537148946674	23.166855108061394
40	24.50426917228742	24.695179799082485	28.692606599347602	22.107944429282494
41	21.327927014698428	24.889339415441796	31.347420952083905	22.43531261777587
42	23.22143813274072	26.537486841592266	27.414843983521127	22.826231042145892
43	20.91166647172729	26.521891691683884	28.422160708019806	24.144281128569016
44	24.549365147439147	24.639427138160034	25.847141538981376	24.964066175419443
45	24.32141603961168	24.94730138926794	27.73090568833093	23.000376882789453
46	20.806139290680598	26.009461057611084	29.43259646250016	23.751803189208157
47	21.703640167907782	26.090945715882363	28.13676946469648	24.06864465151338
48	22.155899515250756	24.19327589119784	30.41197187674633	23.238852716805074
49	22.360195979050516	24.254096975840515	28.956034673216628	24.42967237189234
50	21.178473494743137	23.33580256540216	28.382782954501153	27.10294098535356
51	20.997309836640802	22.797899853145672	30.655776053647315	25.54901425656621
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1152.0
1	952.0
2	752.0
3	552.0
4	352.0
5	360.5
6	369.0
7	418.0
8	467.0
9	553.5
10	640.0
11	733.0
12	826.0
13	877.0
14	928.0
15	1091.5
16	1255.0
17	1343.5
18	1432.0
19	1775.0
20	2118.0
21	2354.5
22	2591.0
23	3322.0
24	4053.0
25	4589.5
26	6425.5
27	7725.0
28	9374.5
29	11024.0
30	13163.5
31	15303.0
32	17691.5
33	20080.0
34	21843.0
35	23606.0
36	26406.0
37	29206.0
38	31128.0
39	33050.0
40	35898.5
41	38747.0
42	39075.0
43	39403.0
44	41841.5
45	44280.0
46	51311.5
47	58343.0
48	65129.5
49	71916.0
50	77970.5
51	84025.0
52	78947.0
53	73869.0
54	63959.5
55	54050.0
56	45980.5
57	37911.0
58	34177.5
59	30444.0
60	27402.0
61	24360.0
62	21291.5
63	18223.0
64	15379.0
65	12535.0
66	9908.0
67	7281.0
68	5690.5
69	4100.0
70	3435.5
71	2771.0
72	2326.5
73	1882.0
74	1662.0
75	1131.0
76	820.0
77	639.5
78	459.0
79	344.0
80	229.0
81	187.0
82	145.0
83	104.5
84	64.0
85	60.5
86	57.0
87	40.0
88	23.0
89	20.5
90	18.0
91	11.5
92	5.0
93	6.0
94	7.0
95	6.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	769470.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.40443441643357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.96627819938945	23.968354109397954
2	10.56624602971839	6.8478645299582235
3	3.76704312195228	3.6620670536753948
4	1.955448273962519	2.534607813933867
5	1.1878550362618958	1.9245885309389463
6	0.8451298500575636	1.6431572879736394
7	0.6636917866619589	1.5054589881529148
8	0.5490112257239484	1.4232318606285994
9	0.48137291269804056	1.4038755281434132
>10	5.39653144345853	39.01253534636043
>50	0.5698777476589854	11.67019161612525
>100	0.04628244400387012	2.6124452723997176
>500	0.0040245603481626185	0.8423414543944576
>1k	8.049120696325237E-4	0.2910831593448567
>5k	4.0245603481626184E-4	0.658197448572353
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	5047	0.6559060132298855	Illumina Single End Adapter 1 (95% over 21bp)
AATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	1190	0.15465190325808673	No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	1042	0.13541788503775326	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	879	0.11423447307887247	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC	833	0.10825633228066071	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGT	826	0.10734661520267197	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7472675997764695	0.0
2	0.0	0.0	0.0	1.0789244544946521	0.0
3	0.0	0.0	0.0	1.425006822878085	0.0
4	0.0	0.0	0.0	2.9366966873302403	0.0
5	0.0	0.0	0.0	3.1981753674607196	0.0
6	0.0	0.0	0.0	4.751972136665497	0.0
7	0.0	0.0	0.0	5.942141993839916	0.0
8	0.0	0.0	0.0	7.062133676426631	0.0
9	0.0	0.0	0.0	8.512612577488401	0.0
10	0.0	0.0	0.0	10.614708825555253	0.0
11	0.0	0.0	0.0	12.445319505633748	0.0
12	0.0	0.0	0.0	13.392984781732881	0.0
13	0.0	0.0	0.0	13.823540878786698	0.0
14	0.0	0.0	0.0	14.266183217019506	0.0
15	0.0	0.0	0.0	14.62695101823333	0.0
16	0.0	0.0	0.0	15.317686199591927	0.0
17	0.0	0.0	0.0	16.27366888897553	0.0
18	0.0	0.0	0.0	17.33205972942415	0.0
19	0.0	0.0	0.0	17.89738391360287	0.0
20	0.0	0.0	0.0	18.478563166855107	0.0
21	0.0	0.0	0.0	19.10886714231874	0.0
22	0.0	0.0	0.0	19.859903569989733	0.0
23	0.0	0.0	0.0	20.5438808530547	0.0
24	0.0	0.0	0.0	21.089841059430515	0.0
25	0.0	0.0	0.0	21.527934812273383	0.0
26	0.0	0.0	0.0	21.947054466061054	0.0
27	0.0	0.0	0.0	22.420237306197773	0.0
28	0.0	0.0	0.0	22.88042418807751	0.0
29	0.0	0.0	0.0	23.3662131077235	0.0
30	0.0	0.0	0.0	24.059547480733492	0.0
31	0.0	0.0	0.0	24.54078781498954	0.0
32	0.0	0.0	0.0	25.0047435247638	0.0
33	0.0	0.0	0.0	25.456612993359066	0.0
34	0.0	0.0	0.0	25.89522658453221	0.0
35	0.0	0.0	0.0	26.37334788880658	0.0
36	0.0	0.0	0.0	26.85289874848922	0.0
37	0.0	0.0	0.0	27.31139615579555	0.0
38	0.0	0.0	0.0	27.748840110725563	0.0
39	0.0	0.0	0.0	28.202918892224517	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCCG	55	1.8189894E-12	45.000004	1
TCTACGG	110	0.0	45.000004	2
GTCGCAC	20	7.0318324E-4	45.0	10
CTCGTAC	20	7.0318324E-4	45.0	31
CGAGTAG	25	3.8896E-5	45.0	1
ACGCACG	50	2.1827873E-11	45.0	1
TTGCTCG	20	7.0318324E-4	45.0	1
CGCGAAG	45	3.8380676E-10	45.0	1
CGTTCCG	20	7.0318324E-4	45.0	1
TCTAGCG	40	6.8102963E-9	45.0	1
CCTCGCG	20	7.0318324E-4	45.0	1
TCGCTAG	20	7.0318324E-4	45.0	1
TTACGAG	50	2.1827873E-11	45.0	1
TTACCGG	85	0.0	45.0	2
TCGACTA	20	7.0318324E-4	45.0	38
GCGTACG	25	3.8896E-5	45.0	1
ACGACCG	25	3.8896E-5	45.0	1
GTTGCGA	20	7.0318324E-4	45.0	31
TACCGGA	20	7.0318324E-4	45.0	3
CTTACCG	25	3.8896E-5	45.0	1
>>END_MODULE