##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553467_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 774940 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80808965855421 33.0 31.0 34.0 30.0 34.0 2 32.00678246057759 33.0 31.0 34.0 30.0 34.0 3 32.15900327767311 34.0 31.0 34.0 30.0 34.0 4 35.76353007974811 37.0 35.0 37.0 35.0 37.0 5 35.589752755052004 37.0 35.0 37.0 33.0 37.0 6 35.60055230082329 37.0 35.0 37.0 33.0 37.0 7 35.783630990786385 37.0 35.0 37.0 35.0 37.0 8 35.943485947299145 37.0 35.0 37.0 35.0 37.0 9 37.530759800758766 39.0 37.0 39.0 35.0 39.0 10 37.01094149224456 39.0 37.0 39.0 33.0 39.0 11 37.00824321882985 39.0 37.0 39.0 33.0 39.0 12 37.031482437349986 39.0 37.0 39.0 33.0 39.0 13 37.00130332670916 39.0 37.0 39.0 33.0 39.0 14 38.197474643198184 40.0 37.0 41.0 33.0 41.0 15 38.170408031589545 40.0 37.0 41.0 33.0 41.0 16 38.120253180891424 40.0 37.0 41.0 33.0 41.0 17 38.09110124654812 40.0 37.0 41.0 33.0 41.0 18 38.05299765143108 40.0 37.0 41.0 33.0 41.0 19 38.05875422613364 40.0 37.0 41.0 33.0 41.0 20 37.981923761839624 40.0 37.0 41.0 33.0 41.0 21 37.94840761865435 40.0 37.0 41.0 33.0 41.0 22 37.95632307017318 40.0 37.0 41.0 33.0 41.0 23 37.86028724804501 40.0 37.0 41.0 33.0 41.0 24 37.766664515962525 40.0 37.0 41.0 33.0 41.0 25 37.77338374583839 40.0 37.0 41.0 33.0 41.0 26 37.69932768988567 40.0 37.0 41.0 33.0 41.0 27 37.66409657521873 40.0 37.0 41.0 33.0 41.0 28 37.62614912122229 40.0 37.0 41.0 32.0 41.0 29 37.48018169148579 39.0 36.0 41.0 32.0 41.0 30 37.46376751748522 39.0 36.0 41.0 32.0 41.0 31 37.38786228611247 39.0 36.0 41.0 32.0 41.0 32 37.36733940692183 39.0 36.0 41.0 32.0 41.0 33 37.22391023821199 39.0 36.0 41.0 31.0 41.0 34 37.22466384494283 39.0 36.0 41.0 31.0 41.0 35 37.06164477249852 39.0 36.0 41.0 31.0 41.0 36 37.06377396959765 39.0 36.0 41.0 31.0 41.0 37 36.967073579890055 39.0 35.0 41.0 31.0 41.0 38 36.92633365163755 39.0 35.0 41.0 31.0 41.0 39 36.800033550984594 39.0 35.0 41.0 30.0 41.0 40 36.71435982140553 39.0 35.0 40.0 30.0 41.0 41 36.71602188556533 39.0 35.0 40.0 30.0 41.0 42 36.673365679923606 39.0 35.0 40.0 30.0 41.0 43 36.65736315069554 39.0 35.0 40.0 30.0 41.0 44 36.65643017523937 39.0 35.0 40.0 30.0 41.0 45 36.472551423336 39.0 35.0 40.0 30.0 41.0 46 36.27852349859344 38.0 35.0 40.0 30.0 41.0 47 36.165605079102896 38.0 35.0 40.0 29.0 41.0 48 36.17233850362609 38.0 35.0 40.0 29.0 41.0 49 36.028844813792034 38.0 35.0 40.0 29.0 41.0 50 35.87216171574573 38.0 34.0 40.0 28.0 41.0 51 34.62834283944564 37.0 33.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 7.0 11 5.0 12 4.0 13 9.0 14 6.0 15 15.0 16 30.0 17 59.0 18 136.0 19 286.0 20 558.0 21 831.0 22 1312.0 23 1791.0 24 2515.0 25 3342.0 26 4336.0 27 5665.0 28 7615.0 29 10186.0 30 13380.0 31 17742.0 32 23360.0 33 31278.0 34 44108.0 35 57319.0 36 72903.0 37 106101.0 38 166690.0 39 203281.0 40 68.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.51730456551475 32.76976282034738 21.244354401631092 27.468578212506774 2 22.73646992025189 31.22061062791958 21.246419077606006 24.79650037422252 3 20.514362402250498 31.33274834180711 20.63953338322967 27.513355872712726 4 20.244664103027333 31.07079257748987 20.170593852427285 28.513949467055514 5 18.654476475598113 33.60053681575348 18.052494386662193 29.69249232198622 6 20.76328489947609 35.543913077141454 19.632358634216843 24.060443389165613 7 86.01659483314837 3.9452086613157147 5.3956435336929305 4.642552971842981 8 87.44225359382662 3.6389914057862542 4.184840116654192 4.733914883732934 9 79.12160941492245 7.8655121686840275 8.42607169587323 4.586806720520299 10 30.568431104343563 42.362763568792424 12.440834129093865 14.627971197770151 11 21.323328257671562 26.78452525356802 34.05231372751439 17.839832761246033 12 24.682556068856947 23.48788293287222 32.449350917490385 19.380210080780447 13 24.52589877926033 24.632487676465274 29.386016981959894 21.4555965623145 14 19.23232766407722 27.579167419413114 31.01504632616719 22.17345859034248 15 17.99558675510362 30.623661186672518 30.392417477482127 20.988334580741736 16 20.513588148759904 28.204635197563682 29.98374067669755 21.298035976978863 17 19.436214416599995 28.312901643998245 30.561978991921958 21.688904947479806 18 20.822773376003305 27.34611711874468 28.626345265439905 23.204764239812114 19 21.076728520917747 30.58172245593207 28.736160218855655 19.605388804294527 20 23.481301778202184 28.62363537822283 28.29251296874597 19.60254987482902 21 21.753038944950575 29.87147392056159 26.76258807133455 21.612899063153275 22 20.6219836374429 26.807107647043644 28.322837897127517 24.24807081838594 23 21.175962009962063 29.576354298397295 25.8769711203448 23.370712571295844 24 18.30838516530312 28.64389501122668 28.651895630629472 24.395824192840738 25 20.1184607840607 29.46279711977702 25.80638501045242 24.61235708570986 26 22.102485353704804 29.61248612795829 26.237128035718893 22.04790048261801 27 19.895088652024672 27.984360079490024 26.64025601982089 25.480295248664415 28 20.797610137559037 28.258703899656744 27.60768575631662 23.336000206467595 29 22.752987328051205 28.612795829354532 25.859550416806464 22.774666425787803 30 21.94350530363641 26.006916664515963 26.49340594110512 25.556172090742507 31 23.88946240999303 28.536531860531138 23.565953493173666 24.008052236302166 32 23.42426510439518 27.24081864402405 25.03019588613312 24.304720365447647 33 21.63419103414458 27.2968229798436 26.323586342168426 24.745399643843395 34 22.22391410947944 26.37210622757891 26.36591219965417 25.03806746328748 35 21.159702686659614 26.120473843136242 28.16747103001523 24.55235244018892 36 22.45257697370119 28.97656592768472 26.495728701576898 22.07512839703719 37 23.804036441530958 27.340439259813664 26.087568069786048 22.76795622886933 38 24.37195137688079 25.526363331354684 28.736289261104087 21.36539603066044 39 23.51304617131649 24.28613828167342 28.98185665987044 23.21895888713965 40 24.49505768188505 24.762949389630165 28.640152786022142 22.101840142462642 41 21.254548739257235 24.940640565721218 31.405012000929105 22.399798694092446 42 23.115854130642372 26.59831728908045 27.513355872712726 22.772472707564457 43 20.768059462668077 26.692260045939044 28.431362428058947 24.108318063333936 44 24.559449763852687 24.78385423387617 25.793093658863913 24.86360234340723 45 24.168193666606445 25.054971997832094 27.670013162309342 23.106821173252122 46 20.787415799932898 26.10034325238083 29.333496786848013 23.778744160838258 47 21.7290370867422 26.09673006942473 28.21341007045707 23.960822773376 48 22.107905128138952 24.289493380132658 30.39628874493509 23.2063127467933 49 22.34405244276976 24.239295945492554 28.92985263375229 24.48679897798539 50 21.344491186414434 23.31199834825922 28.270575786512502 27.072934678813844 51 20.99723849588355 22.828089916638707 30.653728030557204 25.520943556920532 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1122.0 1 936.5 2 751.0 3 528.5 4 306.0 5 339.0 6 372.0 7 410.5 8 449.0 9 534.0 10 619.0 11 710.5 12 802.0 13 869.0 14 936.0 15 1102.5 16 1269.0 17 1371.5 18 1474.0 19 1806.5 20 2139.0 21 2384.5 22 2630.0 23 3376.0 24 4122.0 25 4779.5 26 6731.5 27 8026.0 28 9619.0 29 11212.0 30 13313.5 31 15415.0 32 17823.0 33 20231.0 34 21960.5 35 23690.0 36 26756.5 37 29823.0 38 31557.5 39 33292.0 40 36186.0 41 39080.0 42 39521.0 43 39962.0 44 42449.5 45 44937.0 46 51669.0 47 58401.0 48 65544.5 49 72688.0 50 78085.5 51 83483.0 52 78563.5 53 73644.0 54 64032.0 55 54420.0 56 46256.0 57 38092.0 58 34443.5 59 30795.0 60 27663.0 61 24531.0 62 21448.0 63 18365.0 64 15570.0 65 12775.0 66 10002.5 67 7230.0 68 5642.0 69 4054.0 70 3493.0 71 2932.0 72 2454.5 73 1977.0 74 1759.5 75 1195.0 76 848.0 77 664.5 78 481.0 79 361.5 80 242.0 81 204.5 82 167.0 83 119.5 84 72.0 85 59.0 86 46.0 87 33.5 88 21.0 89 16.0 90 11.0 91 10.5 92 10.0 93 7.5 94 5.0 95 8.5 96 12.0 97 6.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 774940.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.14065234720774 #Duplication Level Percentage of deduplicated Percentage of total 1 73.89288688607698 23.749655883369467 2 10.599428548052856 6.813450960840717 3 3.763874790652026 3.629201733742983 4 1.9243085731217697 2.4739413142983278 5 1.2073015228500745 1.9401729262089367 6 0.8521394724572673 1.6432991121349223 7 0.7033956031812936 1.5825315481083113 8 0.5329871312384091 1.3704443272535438 9 0.484976323389215 1.402870986601178 >10 5.387364595991484 38.678664246398704 >50 0.5953119363090066 12.116162044810745 >100 0.05078490432827763 2.794921310981525 >500 0.004433602758817889 0.97987415046416 >1k 4.0305479625617175E-4 0.16322599545013772 >5k 4.0305479625617175E-4 0.6615834593363915 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT 5107 0.659018762742922 Illumina Single End Adapter 1 (95% over 21bp) AATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT 1260 0.16259323302449222 No Hit CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC 969 0.12504193873074046 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 865 0.11162154489379823 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC 865 0.11162154489379823 No Hit AATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGT 819 0.10568560146591993 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2904224843213668E-4 0.0 0.0 0.7332180555914006 0.0 2 1.2904224843213668E-4 0.0 0.0 1.0369835084006505 0.0 3 1.2904224843213668E-4 0.0 0.0 1.3790745089942447 0.0 4 1.2904224843213668E-4 0.0 0.0 2.879835858259994 0.0 5 1.2904224843213668E-4 0.0 0.0 3.1416625803288 0.0 6 1.2904224843213668E-4 0.0 0.0 4.672232688982373 0.0 7 1.2904224843213668E-4 0.0 0.0 5.840968333032235 0.0 8 1.2904224843213668E-4 0.0 0.0 6.959893669187292 0.0 9 1.2904224843213668E-4 0.0 0.0 8.398069527963456 0.0 10 1.2904224843213668E-4 0.0 0.0 10.530363641056082 0.0 11 1.2904224843213668E-4 0.0 0.0 12.347278498980566 0.0 12 1.2904224843213668E-4 0.0 0.0 13.306836658321934 0.0 13 1.2904224843213668E-4 0.0 0.0 13.735256923116628 0.0 14 1.2904224843213668E-4 0.0 0.0 14.166258032879965 0.0 15 1.2904224843213668E-4 0.0 0.0 14.518414328851266 0.0 16 1.2904224843213668E-4 0.0 0.0 15.199241231579219 0.0 17 1.2904224843213668E-4 0.0 0.0 16.123828941595477 0.0 18 1.2904224843213668E-4 0.0 0.0 17.156941182543164 0.0 19 2.5808449686427336E-4 0.0 0.0 17.70266085116267 0.0 20 2.5808449686427336E-4 0.0 0.0 18.27573747644979 0.0 21 2.5808449686427336E-4 0.0 0.0 18.897463029395823 0.0 22 2.5808449686427336E-4 0.0 0.0 19.64126254935866 0.0 23 2.5808449686427336E-4 0.0 0.0 20.346478437040286 0.0 24 2.5808449686427336E-4 0.0 0.0 20.870648050171628 0.0 25 2.5808449686427336E-4 0.0 0.0 21.31829560998271 0.0 26 2.5808449686427336E-4 0.0 0.0 21.730198466978088 0.0 27 2.5808449686427336E-4 0.0 0.0 22.18687898417942 0.0 28 2.5808449686427336E-4 0.0 0.0 22.65736702196299 0.0 29 2.5808449686427336E-4 0.0 0.0 23.147340439259814 0.0 30 2.5808449686427336E-4 0.0 0.0 23.837716468371745 0.0 31 2.5808449686427336E-4 0.0 0.0 24.33065785738251 0.0 32 2.5808449686427336E-4 0.0 0.0 24.777531163702996 0.0 33 2.5808449686427336E-4 0.0 0.0 25.241179962319663 0.0 34 2.5808449686427336E-4 0.0 0.0 25.70057036673807 0.0 35 2.5808449686427336E-4 0.0 0.0 26.200867163909464 0.0 36 2.5808449686427336E-4 0.0 0.0 26.668387229979096 0.0 37 2.5808449686427336E-4 0.0 0.0 27.118228508013523 0.0 38 2.5808449686427336E-4 0.0 0.0 27.566908405812065 0.0 39 2.5808449686427336E-4 0.0 0.0 28.02268562727437 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 35 1.2112469E-7 45.000004 1 ACCGGTA 20 7.031861E-4 45.000004 4 CTATCCG 70 0.0 45.000004 1 CGGTTTA 20 7.031861E-4 45.000004 19 GCTATCG 20 7.031861E-4 45.000004 1 CGGATAA 20 7.031861E-4 45.000004 36 TCGCTAA 20 7.031861E-4 45.000004 30 ACGGACG 30 2.1645828E-6 45.000004 1 CTTGACG 20 7.031861E-4 45.000004 1 GATATCG 30 2.1645828E-6 45.000004 9 AGCGATC 20 7.031861E-4 45.000004 6 CAGTACG 35 1.2112469E-7 45.000004 1 CGGTAAA 60 0.0 45.000004 15 ATCGCTA 20 7.031861E-4 45.000004 29 GCGTACT 20 7.031861E-4 45.000004 27 TACACCG 20 7.031861E-4 45.000004 1 GTTATCG 20 7.031861E-4 45.000004 18 TACGTAC 20 7.031861E-4 45.000004 33 CGCTTCG 20 7.031861E-4 45.000004 1 TAATCCG 20 7.031861E-4 45.000004 1 >>END_MODULE