SRR3553466_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553466_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	364643
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	5472	1.5006458371612783	TruSeq Adapter, Index 14 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC	2250	0.6170418738327624	No Hit
AATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	2112	0.579196638904353	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGT	1941	0.532301456493063	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1576	0.43220355251574827	No Hit
AAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	1534	0.42068543753753673	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT	1355	0.3715963284637303	No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	1043	0.28603318862558724	TruSeq Adapter, Index 15 (95% over 21bp)
AAAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	712	0.1952594729639675	No Hit
AACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	581	0.15933392386526	No Hit
ACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	552	0.15138093971363772	TruSeq Adapter, Index 14 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT	518	0.14205675139794266	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	488	0.13382952641350582	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	461	0.12642502392751265	No Hit
AACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	409	0.11216450062115549	No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	398	0.10914785146019532	TruSeq Adapter, Index 15 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	369	0.10119486730857305	No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	366	0.10037214481012936	TruSeq Adapter, Index 15 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGGA	30	2.1614105E-6	45.000004	24
CCAATAG	30	2.1614105E-6	45.000004	32
ACGGGCC	85	0.0	45.000004	5
CGGGCTT	30	2.1614105E-6	45.000004	6
CGGGCTA	30	2.1614105E-6	45.000004	6
ACTAACG	30	2.1614105E-6	45.000004	1
ATACCGG	85	0.0	45.000004	2
TCACGAC	20	7.0269447E-4	45.0	25
ACAACGG	35	1.2088822E-7	45.0	2
TTTAGCG	20	7.0269447E-4	45.0	1
CGGGTGC	40	6.7939254E-9	45.0	6
CGTGGGC	25	3.885549E-5	45.0	4
AGGTACG	25	3.885549E-5	45.0	1
CCTTACG	20	7.0269447E-4	45.0	1
AATCCCG	25	3.885549E-5	45.0	1
CGAACTT	20	7.0269447E-4	45.0	45
ATTAGAC	20	7.0269447E-4	45.0	14
GATCGGA	25	3.885549E-5	45.0	9
ATCTACG	40	6.7939254E-9	45.0	1
CTCCGAC	20	7.0269447E-4	45.0	26

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content