##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553466_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	364643
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.566828377344418	31.0	31.0	34.0	30.0	34.0
2	31.782968547318884	31.0	31.0	34.0	30.0	34.0
3	31.946534007234472	33.0	31.0	34.0	30.0	34.0
4	35.621783497832126	37.0	35.0	37.0	33.0	37.0
5	35.39393050188815	37.0	35.0	37.0	33.0	37.0
6	35.385182219321365	37.0	35.0	37.0	33.0	37.0
7	35.450218432823334	37.0	35.0	37.0	33.0	37.0
8	35.70826808686853	37.0	35.0	37.0	35.0	37.0
9	37.297000079529845	39.0	37.0	39.0	34.0	39.0
10	36.8181838126606	39.0	37.0	39.0	32.0	39.0
11	36.82234130368607	39.0	37.0	39.0	32.0	39.0
12	36.80137833442573	39.0	37.0	39.0	32.0	39.0
13	36.75139794264527	39.0	35.0	39.0	32.0	39.0
14	37.820843948738904	39.0	37.0	41.0	33.0	41.0
15	37.83990642902784	39.0	37.0	41.0	33.0	41.0
16	37.7741133108273	39.0	37.0	41.0	33.0	41.0
17	37.7829411232356	39.0	37.0	41.0	33.0	41.0
18	37.72731959752415	39.0	37.0	41.0	33.0	41.0
19	37.65470336740319	39.0	36.0	41.0	32.0	41.0
20	37.627995052695375	39.0	36.0	41.0	32.0	41.0
21	37.55383484668566	39.0	36.0	41.0	32.0	41.0
22	37.554915355566955	39.0	36.0	41.0	32.0	41.0
23	37.418118543342395	39.0	36.0	40.0	32.0	41.0
24	37.33081397421588	39.0	36.0	41.0	32.0	41.0
25	37.243780903513844	39.0	36.0	41.0	31.0	41.0
26	37.21785417517956	39.0	36.0	40.0	32.0	41.0
27	37.185474560049144	39.0	36.0	40.0	31.0	41.0
28	37.001173750764444	39.0	36.0	40.0	31.0	41.0
29	36.965209807949144	39.0	36.0	40.0	31.0	41.0
30	36.859177332349724	39.0	35.0	40.0	31.0	41.0
31	36.70253371105437	39.0	35.0	40.0	30.0	41.0
32	36.72340343843156	39.0	35.0	40.0	30.0	41.0
33	36.62924010607635	39.0	35.0	40.0	30.0	41.0
34	36.43950932830193	39.0	35.0	40.0	30.0	41.0
35	36.407063895371635	39.0	35.0	40.0	30.0	41.0
36	36.36745529188823	39.0	35.0	40.0	30.0	41.0
37	36.34866156761545	39.0	35.0	40.0	30.0	41.0
38	36.38617222872782	39.0	35.0	40.0	30.0	41.0
39	36.243802842780475	39.0	35.0	40.0	30.0	41.0
40	35.98245407151652	38.0	35.0	40.0	29.0	41.0
41	35.879646668111	38.0	35.0	40.0	29.0	41.0
42	35.75697325877639	38.0	35.0	40.0	28.0	41.0
43	35.76622614447555	38.0	35.0	40.0	28.0	41.0
44	35.641262824186946	38.0	35.0	40.0	28.0	41.0
45	35.41303960311867	38.0	34.0	40.0	26.0	41.0
46	35.21916778876874	38.0	34.0	40.0	26.0	41.0
47	35.11280896657827	38.0	34.0	40.0	26.0	41.0
48	35.01879372427278	38.0	34.0	40.0	26.0	41.0
49	34.849222390118555	38.0	33.0	40.0	26.0	41.0
50	34.68928239401277	38.0	33.0	40.0	24.0	41.0
51	33.25942360061759	36.0	31.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	2.0
12	2.0
13	4.0
14	6.0
15	13.0
16	25.0
17	56.0
18	142.0
19	256.0
20	482.0
21	718.0
22	1088.0
23	1486.0
24	1986.0
25	2354.0
26	3056.0
27	3817.0
28	4873.0
29	6134.0
30	8039.0
31	10243.0
32	13192.0
33	17172.0
34	23832.0
35	29876.0
36	36153.0
37	50662.0
38	74584.0
39	74372.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.622597993105586	35.038105763719585	19.49934593561374	26.839950307561093
2	19.04136374481342	35.38447193556438	20.26475209999918	25.309412219623027
3	18.852960292669817	33.59888987310877	23.30717989924392	24.2409699349775
4	19.59807263542698	32.26251429480341	20.49346895456652	27.645944115203093
5	16.686457713434784	36.22748825563634	18.91137359005932	28.174680440869565
6	20.570256387754597	35.246254555825836	21.119012294216535	23.064476762203032
7	78.49129148235397	6.3755508812729165	8.423307179899243	6.709850456473866
8	80.54562956096785	4.413357722484731	6.3053452280723885	8.73566748847503
9	72.30716070238562	8.045129071447963	13.362110338056674	6.28559988810974
10	30.217774645338043	30.893229816560307	16.557838762844753	22.331156775256893
11	20.66404675257718	29.245042411344794	31.322965201580722	18.767945634497302
12	23.147023252880214	23.23203791105273	33.87861552257962	19.74232331348744
13	24.316111923168688	25.36234070035624	28.911839799475104	21.40970757699997
14	16.74569373332273	27.16684538027605	34.83461906577118	21.252841820630042
15	15.719484536930642	29.89691287094501	31.2980092857946	23.085593306329752
16	17.228631839909173	28.751408912278585	29.364885655284755	24.655073592527486
17	17.825105651280843	28.818049434652522	30.605002701272205	22.751842212794433
18	18.65770081970585	25.605043837397123	29.36515989611757	26.372095446779454
19	18.76849411616293	31.222867297603408	30.652446365349125	19.356192220884534
20	20.09417430198852	29.164964088162943	28.92061550612517	21.820246103723367
21	18.47917003754357	30.114111610534138	27.94184997381	23.464868378112293
22	17.568690472599226	27.97777552290871	28.829293308797922	25.624240695694144
23	19.393488974147317	27.62866694273577	26.255543092833268	26.72230099028365
24	15.547261293923098	30.42290678828333	26.820753449264075	27.20907846852949
25	18.39634930603357	29.778715072001933	26.299695866916405	25.52523975504809
26	20.590824450215692	29.048685975049573	26.18122382714052	24.179265747594222
27	16.119053430341456	27.460557312220445	26.476306963248987	29.944082294189112
28	19.46534007234473	27.15423030196658	29.17977309313493	24.200656532553758
29	19.483714208143308	27.225807159331183	25.013232120183304	28.27724651234221
30	21.143968210002658	27.174524123594857	23.50573026220166	28.17577740420082
31	19.125829921320307	28.485121063615647	25.602575669901796	26.786473345162253
32	19.280775991860533	25.083163532551016	24.088492031932603	31.547568443655848
33	21.33237166214626	25.767394410423343	26.818833763434373	26.081400163996015
34	16.11493981784924	24.986905000233104	28.185650074182146	30.71250510773551
35	19.13625107296726	24.597757258469244	27.85656107480467	28.409430593758827
36	18.696917258798333	22.551097923174172	32.1681754483152	26.58380936971229
37	17.64465518328886	24.3879630213661	29.35830387529721	28.609077920047827
38	20.581500261899997	23.4481396873106	31.621887709348595	24.348472341440807
39	21.1974451724015	21.151921194154284	30.982632328057853	26.668001305386362
40	23.703732143493774	23.954114023853467	28.894562627007787	23.447591205644976
41	18.71419443126565	21.536406841760297	35.47277748373066	24.27662124324339
42	22.2088453638216	24.416209827146005	29.506119684184256	23.86882512484814
43	19.475761223991686	21.90992285605373	30.8139742158769	27.800341704077685
44	21.36473208041838	21.176602869107594	27.456992181393858	30.00167286908017
45	23.923673291411053	21.641441080728274	30.18212333707215	24.252762290788525
46	19.04438039397438	21.754976785513502	33.128841085664604	26.07180173484751
47	21.79117657544502	22.748551322800655	28.263260229868663	27.19701187188565
48	20.060716920385143	19.880540693225974	35.92061276371684	24.13812962267204
49	21.38996223703732	19.060286362277626	31.623533154345484	27.92621824633957
50	19.69734781690585	18.961011180798753	28.842731109605836	32.49890989268957
51	20.079639537849346	18.21946396886818	34.96433497969247	26.736561513590008
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1881.0
1	1466.5
2	1052.0
3	734.5
4	417.0
5	398.5
6	380.0
7	397.0
8	414.0
9	440.0
10	466.0
11	526.0
12	586.0
13	654.5
14	723.0
15	780.5
16	838.0
17	883.0
18	928.0
19	963.5
20	999.0
21	1233.0
22	1467.0
23	1515.0
24	1563.0
25	1625.0
26	2021.0
27	2355.0
28	2868.0
29	3381.0
30	4093.0
31	4805.0
32	5698.5
33	6592.0
34	7156.5
35	7721.0
36	7874.5
37	8028.0
38	9025.5
39	10023.0
40	11636.0
41	13249.0
42	16992.0
43	20735.0
44	27448.0
45	34161.0
46	38274.5
47	42388.0
48	45784.5
49	49181.0
50	45112.0
51	41043.0
52	35985.5
53	30928.0
54	26674.5
55	22421.0
56	18482.0
57	14543.0
58	13158.5
59	11774.0
60	10158.0
61	8542.0
62	7299.0
63	6056.0
64	5218.5
65	4381.0
66	3743.0
67	3105.0
68	2646.5
69	2188.0
70	1829.0
71	1470.0
72	1111.5
73	753.0
74	610.0
75	430.0
76	393.0
77	309.0
78	225.0
79	216.0
80	207.0
81	138.5
82	70.0
83	44.0
84	18.0
85	19.5
86	21.0
87	15.5
88	10.0
89	6.5
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	364643.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.73351293494823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.46316484327153	25.863672992609544
2	11.121031002624886	7.725449483592635
3	4.3008185718243315	4.48147612485978
4	2.1838502602193737	3.0341116504527874
5	1.2739545315600278	2.212445810023807
6	0.8389397398134015	1.7483594582670532
7	0.5815585192502915	1.4139699245566535
8	0.47866268506424414	1.330050925052478
9	0.38846073967238276	1.2143345513517236
>10	3.8141671146690905	30.394969733320348
>50	0.4802321070690831	10.807197110073627
>100	0.06566600475573953	4.375310017966647
>500	0.003164626735216363	0.6493441510146456
>1k	0.005538096786628635	3.2456215690368087
>5k	7.911566838040907E-4	1.5036864978214728
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	5472	1.5006458371612783	TruSeq Adapter, Index 14 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC	2250	0.6170418738327624	No Hit
AATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	2112	0.579196638904353	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGT	1941	0.532301456493063	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1576	0.43220355251574827	No Hit
AAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	1534	0.42068543753753673	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT	1355	0.3715963284637303	No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	1043	0.28603318862558724	TruSeq Adapter, Index 15 (95% over 21bp)
AAAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	712	0.1952594729639675	No Hit
AACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	581	0.15933392386526	No Hit
ACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	552	0.15138093971363772	TruSeq Adapter, Index 14 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT	518	0.14205675139794266	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	488	0.13382952641350582	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	461	0.12642502392751265	No Hit
AACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	409	0.11216450062115549	No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	398	0.10914785146019532	TruSeq Adapter, Index 15 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	369	0.10119486730857305	No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	366	0.10037214481012936	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.7968259366010042	0.0
2	0.0	0.0	0.0	2.4681674953310497	0.0
3	0.0	0.0	0.0	3.161722561519075	0.0
4	0.0	0.0	0.0	6.524463653491223	0.0
5	0.0	0.0	0.0	7.011789613402698	0.0
6	0.0	0.0	0.0	9.9310832787137	0.0
7	0.0	0.0	0.0	12.069613293001648	0.0
8	0.0	0.0	0.0	13.683246353282525	0.0
9	0.0	0.0	0.0	16.26824044339258	0.0
10	0.0	0.0	0.0	19.72614310435138	0.0
11	0.0	0.0	0.0	23.116308279604983	0.0
12	0.0	0.0	0.0	24.641087310053944	0.0
13	0.0	0.0	0.0	25.258677665552334	0.0
14	0.0	0.0	0.0	25.929744983449567	0.0
15	0.0	0.0	0.0	26.490019004889714	0.0
16	0.0	0.0	0.0	27.649235005196864	0.0
17	0.0	0.0	0.0	29.05636471836838	0.0
18	0.0	0.0	0.0	31.12057546696358	0.0
19	0.0	0.0	0.0	31.88954676217561	0.0
20	0.0	0.0	0.0	32.80962475626846	0.0
21	0.0	0.0	0.0	33.69734233208919	0.0
22	0.0	0.0	0.0	34.59246441039592	0.0
23	0.0	0.0	0.0	35.46208209125089	0.0
24	0.0	0.0	0.0	36.08076941008054	0.0
25	0.0	0.0	0.0	36.628702594044036	0.0
26	0.0	0.0	0.0	37.11246342312892	0.0
27	0.0	0.0	0.0	37.671640481237816	0.0
28	0.0	0.0	0.0	38.20421617856369	0.0
29	0.0	0.0	0.0	38.771346220824206	0.0
30	0.0	0.0	0.0	39.53565542187839	0.0
31	0.0	0.0	0.0	40.05616452256042	0.0
32	0.0	0.0	0.0	40.54184503747501	0.0
33	0.0	0.0	0.0	41.000375709940954	0.0
34	0.0	0.0	0.0	41.477280518205475	0.0
35	0.0	0.0	0.0	41.976124593095165	0.0
36	0.0	0.0	0.0	42.455497568855016	0.0
37	0.0	0.0	0.0	42.91320551882252	0.0
38	0.0	0.0	0.0	43.348699961332045	0.0
39	0.0	0.0	0.0	43.80339126213858	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGGA	30	2.1614105E-6	45.000004	24
CCAATAG	30	2.1614105E-6	45.000004	32
ACGGGCC	85	0.0	45.000004	5
CGGGCTT	30	2.1614105E-6	45.000004	6
CGGGCTA	30	2.1614105E-6	45.000004	6
ACTAACG	30	2.1614105E-6	45.000004	1
ATACCGG	85	0.0	45.000004	2
TCACGAC	20	7.0269447E-4	45.0	25
ACAACGG	35	1.2088822E-7	45.0	2
TTTAGCG	20	7.0269447E-4	45.0	1
CGGGTGC	40	6.7939254E-9	45.0	6
CGTGGGC	25	3.885549E-5	45.0	4
AGGTACG	25	3.885549E-5	45.0	1
CCTTACG	20	7.0269447E-4	45.0	1
AATCCCG	25	3.885549E-5	45.0	1
CGAACTT	20	7.0269447E-4	45.0	45
ATTAGAC	20	7.0269447E-4	45.0	14
GATCGGA	25	3.885549E-5	45.0	9
ATCTACG	40	6.7939254E-9	45.0	1
CTCCGAC	20	7.0269447E-4	45.0	26
>>END_MODULE