FastQCFastQC Report
Sat 18 Jun 2016
SRR3553465_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553465_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365939
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT56051.5316760443680504TruSeq Adapter, Index 14 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC22300.6093911826834527No Hit
AATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT21930.599280207903503No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGT20000.5465391772945764No Hit
AAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT15370.420015357750882No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15170.4145499659779362No Hit
AAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT14560.39788052107045163No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC10350.2828340242499433TruSeq Adapter, Index 15 (95% over 21bp)
AAAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT7600.20768488737193902No Hit
AACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT5880.16068251812460546No Hit
AATGACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT5460.14920519540141938No Hit
ACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG5180.14155364691929528TruSeq Adapter, Index 14 (95% over 21bp)
GAAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT4790.13089613296205105No Hit
AATGATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT4630.12652381954369443No Hit
AACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG4130.11286034011133003No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC4010.10958110504756258TruSeq Adapter, Index 15 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3920.10712167874973699No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC3920.10712167874973699TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG207.0269755E-445.0000041
TCACGAC351.2089004E-745.00000425
TTTAGCG207.0269755E-445.0000041
CGGGTGC207.0269755E-445.0000046
AGGTACG207.0269755E-445.0000041
CTCACGA351.2089004E-745.00000424
GTACCGG207.0269755E-445.0000042
GCGACTC406.7939254E-945.00000417
CAACGCG207.0269755E-445.0000041
CATGAGT351.2089004E-745.00000439
CCAATAC207.0269755E-445.00000423
ACCGAGA207.0269755E-445.00000439
CACGCAA207.0269755E-445.00000410
GTTAGAT207.0269755E-445.00000443
ATTCGAA207.0269755E-445.00000425
CTAAGCG207.0269755E-445.0000041
TACGGGT406.7939254E-945.0000044
CGCGACT406.7939254E-945.00000416
TGACGAG207.0269755E-445.00000442
TCGGATC207.0269755E-445.00000431
Produced by FastQC (version 0.11.5)