##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553465_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	365939
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.539885062811013	31.0	31.0	34.0	30.0	34.0
2	31.74768745610607	31.0	31.0	34.0	30.0	34.0
3	31.923694932762018	33.0	31.0	34.0	30.0	34.0
4	35.564391879520905	37.0	35.0	37.0	33.0	37.0
5	35.369291056706174	37.0	35.0	37.0	33.0	37.0
6	35.374805637005075	37.0	35.0	37.0	33.0	37.0
7	35.461631583405975	37.0	35.0	37.0	33.0	37.0
8	35.72019653548816	37.0	35.0	37.0	35.0	37.0
9	37.28543281803798	39.0	37.0	39.0	34.0	39.0
10	36.803390729055934	39.0	37.0	39.0	32.0	39.0
11	36.815625008539676	39.0	37.0	39.0	32.0	39.0
12	36.80674374690864	39.0	37.0	39.0	32.0	39.0
13	36.72906140094387	39.0	35.0	39.0	32.0	39.0
14	37.81240589278541	39.0	37.0	41.0	33.0	41.0
15	37.81716351632376	39.0	37.0	41.0	33.0	41.0
16	37.778525929184916	39.0	37.0	41.0	33.0	41.0
17	37.76329388231372	39.0	37.0	41.0	33.0	41.0
18	37.681567146436976	39.0	37.0	41.0	33.0	41.0
19	37.62101060559274	39.0	36.0	41.0	32.0	41.0
20	37.5916641844679	39.0	36.0	41.0	32.0	41.0
21	37.52950628383419	39.0	36.0	41.0	32.0	41.0
22	37.50908211477869	39.0	36.0	41.0	32.0	41.0
23	37.40122807353138	39.0	36.0	40.0	32.0	41.0
24	37.32474264836489	39.0	36.0	41.0	32.0	41.0
25	37.24301591248815	39.0	36.0	41.0	31.0	41.0
26	37.19046343789539	39.0	36.0	40.0	31.0	41.0
27	37.13332276690924	39.0	36.0	40.0	31.0	41.0
28	36.964712698017976	39.0	36.0	40.0	31.0	41.0
29	36.921161723675255	39.0	36.0	40.0	31.0	41.0
30	36.84484845834962	39.0	35.0	40.0	31.0	41.0
31	36.68535466293562	39.0	35.0	40.0	30.0	41.0
32	36.724098825214035	39.0	35.0	40.0	30.0	41.0
33	36.63371217607306	39.0	35.0	40.0	30.0	41.0
34	36.42614206192835	39.0	35.0	40.0	30.0	41.0
35	36.38136410713261	39.0	35.0	40.0	30.0	41.0
36	36.38796356769844	39.0	35.0	40.0	30.0	41.0
37	36.302782704221194	38.0	35.0	40.0	30.0	41.0
38	36.31132511156231	39.0	35.0	40.0	30.0	41.0
39	36.15857287690025	38.0	35.0	40.0	30.0	41.0
40	35.9485870595919	38.0	35.0	40.0	29.0	41.0
41	35.8937609820216	38.0	35.0	40.0	29.0	41.0
42	35.72953415733223	38.0	35.0	40.0	28.0	41.0
43	35.73751636201662	38.0	35.0	40.0	28.0	41.0
44	35.69080639122914	38.0	35.0	40.0	28.0	41.0
45	35.45427516607959	38.0	34.0	40.0	27.0	41.0
46	35.250719382192116	38.0	34.0	40.0	26.0	41.0
47	35.110078455698904	38.0	34.0	40.0	26.0	41.0
48	35.06731996316326	38.0	34.0	40.0	26.0	41.0
49	34.92864931040419	38.0	34.0	40.0	26.0	41.0
50	34.721273217667424	38.0	33.0	40.0	24.0	41.0
51	33.32293087099216	36.0	31.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	1.0
11	0.0
12	0.0
13	2.0
14	6.0
15	14.0
16	28.0
17	54.0
18	127.0
19	297.0
20	474.0
21	807.0
22	1061.0
23	1516.0
24	1949.0
25	2447.0
26	3050.0
27	3780.0
28	4835.0
29	6309.0
30	8130.0
31	10349.0
32	13363.0
33	17346.0
34	23710.0
35	30135.0
36	36429.0
37	50310.0
38	75260.0
39	74132.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.65994059119143	34.944895187449276	19.531943848564925	26.863220372794373
2	18.95288559022132	35.295773339272394	20.448216779299283	25.303124291207002
3	19.062466695268885	33.36703658259984	23.35553193291778	24.214964789213504
4	19.604633559145103	32.44721114721306	20.409685767300015	27.538469526341824
5	16.717267085497856	36.307144086856006	18.905883220973987	28.06970560667215
6	20.478003164461835	35.29741295680427	21.060613927457855	23.16396995127603
7	78.40104498290698	6.433312655934459	8.429547001002899	6.736095360155654
8	80.46614326431455	4.458666608369154	6.351058509751625	8.724131617564677
9	72.1877143458336	8.016090113379553	13.461806475942712	6.334389064844141
10	30.15666545517149	30.84585135773995	16.63446640013773	22.363016786950833
11	20.766302580484723	29.09310021615624	31.445131565643454	18.695465637715575
12	23.042911523505282	23.503370780375967	33.83569392712993	19.61802376898882
13	24.21305190209297	25.411885587488626	28.769549023197854	21.60551348722055
14	16.70660957154061	27.243065101014103	34.90335820997489	21.146967117470396
15	15.638125479929716	30.069219186804357	31.225149546782387	23.06750578648354
16	17.144933991730863	28.99472316424322	29.381126362590486	24.479216481435433
17	17.72945764184741	29.03707995048355	30.46901259499534	22.7644498126737
18	18.5869776110226	25.70974943911417	29.388777911072612	26.314495038790618
19	18.668958487616788	31.323526598695413	30.650190332268494	19.35732458141931
20	20.096245549121576	29.26990564001104	28.89388668603237	21.739962124835014
21	18.518933483449427	30.102558076619328	27.817204506762057	23.56130393316919
22	17.519040058589	28.150046865734453	28.809446383140358	25.521466692536187
23	19.487674175204063	27.65378929275098	26.26312035612493	26.59541617592003
24	15.572814048243014	30.500711867278422	26.742708484200918	27.183765600277642
25	18.315620909495845	29.800595181164073	26.30329098565608	25.580492923684
26	20.58539811280022	29.16196415249536	26.02674216194502	24.225895572759395
27	16.25325532397476	27.418504176925662	26.412598821114997	29.915641677984585
28	19.515820942834736	27.331604447735824	29.072878266596348	24.079696342833095
29	19.53631616198328	27.30236460175057	24.93475688571046	28.226562350555696
30	21.157078092250348	27.22011045556773	23.54408794908441	28.078723503097514
31	19.126958318189644	28.564050292535093	25.571201757669993	26.737789631605267
32	19.292832958498547	25.02056353654571	24.071498255173676	31.615105249782065
33	21.400834565323727	25.643071659484228	26.848463815007417	26.10762996018462
34	16.073717204233493	24.902237804661436	28.21453848865521	30.809506502449864
35	19.144174302274422	24.47948975102408	27.93662331700092	28.43971262970058
36	18.58725088061125	22.616064426038218	32.323146753967194	26.47353793938334
37	17.633266746643567	24.541248678058363	29.3245595577405	28.50092501755757
38	20.681589008004067	23.497632119014373	31.647897600419743	24.17288127256182
39	21.07318432853563	21.32022003667278	30.97920691699983	26.62738871779176
40	23.72362606882568	23.940328852622976	28.814911774913305	23.521133303638038
41	18.744107624494795	21.555505152498096	35.349880717824554	24.350506505182558
42	22.31382826099432	24.457628183932297	29.35024689907334	23.878296656000046
43	19.48931379273595	22.03454674139679	30.746654497060987	27.72948496880628
44	21.435266533493287	21.273764206602742	27.446650944556332	29.84431831534764
45	24.0739576814715	21.624095819248563	30.08616190130049	24.215784597979447
46	18.967915417596924	21.76264350069274	33.2038946381774	26.065546443532938
47	21.78969718996882	22.7592576904894	28.144034934784212	27.30701018475757
48	20.041864900980766	19.85303561522549	35.859801770240395	24.245297713553352
49	21.49839180847081	18.986771019213585	31.571655385187146	27.943181787128456
50	19.709295811597013	18.944960772150548	28.860821065806046	32.48492235044639
51	19.97682673888271	18.27681662790793	35.049557439901186	26.696799193308173
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1823.0
1	1460.0
2	1097.0
3	776.0
4	455.0
5	406.0
6	357.0
7	369.0
8	381.0
9	429.0
10	477.0
11	521.0
12	565.0
13	659.5
14	754.0
15	789.0
16	824.0
17	907.5
18	991.0
19	1008.5
20	1026.0
21	1283.0
22	1540.0
23	1605.5
24	1671.0
25	1748.0
26	2064.0
27	2303.0
28	2865.0
29	3427.0
30	4102.5
31	4778.0
32	5728.0
33	6678.0
34	7204.5
35	7731.0
36	7964.5
37	8198.0
38	9174.0
39	10150.0
40	11662.0
41	13174.0
42	16915.0
43	20656.0
44	27488.5
45	34321.0
46	38180.5
47	42040.0
48	45865.0
49	49690.0
50	45522.5
51	41355.0
52	36263.5
53	31172.0
54	26634.5
55	22097.0
56	18512.5
57	14928.0
58	13189.0
59	11450.0
60	9961.0
61	8472.0
62	7224.5
63	5977.0
64	5236.0
65	4495.0
66	3822.0
67	3149.0
68	2715.0
69	2281.0
70	1897.5
71	1514.0
72	1146.5
73	779.0
74	610.5
75	409.0
76	376.0
77	284.5
78	193.0
79	200.5
80	208.0
81	137.5
82	67.0
83	46.0
84	25.0
85	17.5
86	10.0
87	10.5
88	11.0
89	6.5
90	2.0
91	1.0
92	0.0
93	1.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	365939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.56031612248505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.40283031002606	25.71385335922113
2	11.208510148127393	7.747393079627288
3	4.311593921233968	4.470301467288924
4	2.1049026790906917	2.9098440798576
5	1.2489568775484639	2.1582172255713377
6	0.8534305747215913	1.7696898270583379
7	0.5951807080126883	1.4398743393246087
8	0.4780091181277046	1.3216116985539015
9	0.3869898565722042	1.2037042601397474
>10	3.822388426636756	30.060119249078877
>50	0.5096516882172679	11.264304356872637
>100	0.06805907637684012	4.475089180214886
>500	0.003165538436132099	0.6596941145751317
>1k	0.005539692263231173	3.2733081107940203
>5k	7.913846090330248E-4	1.5329956518215644
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	5605	1.5316760443680504	TruSeq Adapter, Index 14 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC	2230	0.6093911826834527	No Hit
AATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	2193	0.599280207903503	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGT	2000	0.5465391772945764	No Hit
AAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	1537	0.420015357750882	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1517	0.4145499659779362	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT	1456	0.39788052107045163	No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	1035	0.2828340242499433	TruSeq Adapter, Index 15 (95% over 21bp)
AAAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	760	0.20768488737193902	No Hit
AACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	588	0.16068251812460546	No Hit
AATGACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT	546	0.14920519540141938	No Hit
ACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	518	0.14155364691929528	TruSeq Adapter, Index 14 (95% over 21bp)
GAAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	479	0.13089613296205105	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	463	0.12652381954369443	No Hit
AACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	413	0.11286034011133003	No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	401	0.10958110504756258	TruSeq Adapter, Index 15 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	392	0.10712167874973699	No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	392	0.10712167874973699	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.732695886472882E-4	0.0	0.0	1.8041258242493967	0.0
2	2.732695886472882E-4	0.0	0.0	2.4733630468466057	0.0
3	2.732695886472882E-4	0.0	0.0	3.1633687581810084	0.0
4	2.732695886472882E-4	0.0	0.0	6.538521447563665	0.0
5	2.732695886472882E-4	0.0	0.0	7.029313628774195	0.0
6	2.732695886472882E-4	0.0	0.0	9.976252872746551	0.0
7	2.732695886472882E-4	0.0	0.0	12.148199563315197	0.0
8	2.732695886472882E-4	0.0	0.0	13.78453786013516	0.0
9	2.732695886472882E-4	0.0	0.0	16.412298224567483	0.0
10	2.732695886472882E-4	0.0	0.0	19.826255195538053	0.0
11	2.732695886472882E-4	0.0	0.0	23.206873276693656	0.0
12	2.732695886472882E-4	0.0	0.0	24.672418080609063	0.0
13	2.732695886472882E-4	0.0	0.0	25.292193507661114	0.0
14	2.732695886472882E-4	0.0	0.0	25.948040520414605	0.0
15	2.732695886472882E-4	0.0	0.0	26.512615490559902	0.0
16	2.732695886472882E-4	0.0	0.0	27.63548023031161	0.0
17	2.732695886472882E-4	0.0	0.0	29.02587589734901	0.0
18	2.732695886472882E-4	0.0	0.0	31.063920489480488	0.0
19	2.732695886472882E-4	0.0	0.0	31.822243597976712	0.0
20	2.732695886472882E-4	0.0	0.0	32.734964024058655	0.0
21	2.732695886472882E-4	0.0	0.0	33.62254364798505	0.0
22	2.732695886472882E-4	0.0	0.0	34.51230942862062	0.0
23	2.732695886472882E-4	0.0	0.0	35.36135804054774	0.0
24	2.732695886472882E-4	0.0	0.0	35.971569031997134	0.0
25	2.732695886472882E-4	0.0	0.0	36.49925260767505	0.0
26	2.732695886472882E-4	0.0	0.0	37.014912321452485	0.0
27	2.732695886472882E-4	0.0	0.0	37.60654098087386	0.0
28	2.732695886472882E-4	0.0	0.0	38.15471977570032	0.0
29	2.732695886472882E-4	0.0	0.0	38.7162887803705	0.0
30	2.732695886472882E-4	0.0	0.0	39.479530741462376	0.0
31	2.732695886472882E-4	0.0	0.0	40.01814510068618	0.0
32	2.732695886472882E-4	0.0	0.0	40.469039921954206	0.0
33	2.732695886472882E-4	0.0	0.0	40.941523040725365	0.0
34	2.732695886472882E-4	0.0	0.0	41.425756751808365	0.0
35	2.732695886472882E-4	0.0	0.0	41.92037470725995	0.0
36	2.732695886472882E-4	0.0	0.0	42.37536857235769	0.0
37	2.732695886472882E-4	0.0	0.0	42.81943165390953	0.0
38	2.732695886472882E-4	0.0	0.0	43.25338376068142	0.0
39	2.732695886472882E-4	0.0	0.0	43.72012821809099	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	20	7.0269755E-4	45.000004	1
TCACGAC	35	1.2089004E-7	45.000004	25
TTTAGCG	20	7.0269755E-4	45.000004	1
CGGGTGC	20	7.0269755E-4	45.000004	6
AGGTACG	20	7.0269755E-4	45.000004	1
CTCACGA	35	1.2089004E-7	45.000004	24
GTACCGG	20	7.0269755E-4	45.000004	2
GCGACTC	40	6.7939254E-9	45.000004	17
CAACGCG	20	7.0269755E-4	45.000004	1
CATGAGT	35	1.2089004E-7	45.000004	39
CCAATAC	20	7.0269755E-4	45.000004	23
ACCGAGA	20	7.0269755E-4	45.000004	39
CACGCAA	20	7.0269755E-4	45.000004	10
GTTAGAT	20	7.0269755E-4	45.000004	43
ATTCGAA	20	7.0269755E-4	45.000004	25
CTAAGCG	20	7.0269755E-4	45.000004	1
TACGGGT	40	6.7939254E-9	45.000004	4
CGCGACT	40	6.7939254E-9	45.000004	16
TGACGAG	20	7.0269755E-4	45.000004	42
TCGGATC	20	7.0269755E-4	45.000004	31
>>END_MODULE