##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553464_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	675952
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61520788458352	31.0	31.0	34.0	30.0	34.0
2	31.812016237839373	33.0	31.0	34.0	30.0	34.0
3	31.98740295168888	33.0	31.0	34.0	30.0	34.0
4	35.72617434374038	37.0	35.0	37.0	33.0	37.0
5	35.45719666485194	37.0	35.0	37.0	33.0	37.0
6	34.11794920349374	37.0	35.0	37.0	32.0	37.0
7	34.973357575685846	37.0	35.0	37.0	32.0	37.0
8	35.65999065022368	37.0	35.0	37.0	33.0	37.0
9	37.35135187113878	39.0	37.0	39.0	34.0	39.0
10	36.665891956825334	38.0	37.0	39.0	32.0	39.0
11	36.6842142637347	39.0	37.0	39.0	32.0	39.0
12	36.80204067744455	39.0	37.0	39.0	32.0	39.0
13	36.801619049873366	39.0	37.0	39.0	32.0	39.0
14	37.99153638128151	40.0	37.0	41.0	33.0	41.0
15	37.94183758610079	40.0	37.0	41.0	33.0	41.0
16	37.833568064004545	40.0	37.0	41.0	33.0	41.0
17	37.803318874713	39.0	37.0	41.0	33.0	41.0
18	37.6364712287263	39.0	37.0	41.0	33.0	41.0
19	37.56501201268729	39.0	36.0	41.0	32.0	41.0
20	37.473245141666865	39.0	36.0	41.0	32.0	41.0
21	37.44388506876228	39.0	36.0	41.0	32.0	41.0
22	37.43210020829881	39.0	36.0	41.0	32.0	41.0
23	37.25753012048193	39.0	35.0	40.0	32.0	41.0
24	37.08935545719223	39.0	35.0	40.0	31.0	41.0
25	37.13039387412124	39.0	35.0	40.0	32.0	41.0
26	37.053665053139866	39.0	35.0	40.0	32.0	41.0
27	37.01318288872583	39.0	35.0	40.0	31.0	41.0
28	36.93731655502166	39.0	35.0	40.0	31.0	41.0
29	36.821043506047765	39.0	35.0	40.0	31.0	41.0
30	36.75754343503681	39.0	35.0	40.0	31.0	41.0
31	36.701870842900085	39.0	35.0	40.0	31.0	41.0
32	36.60299399957393	39.0	35.0	40.0	31.0	41.0
33	36.5288496816342	39.0	35.0	40.0	30.0	41.0
34	36.478242537931685	39.0	35.0	40.0	30.0	41.0
35	36.313281120552936	38.0	35.0	40.0	30.0	41.0
36	36.29796346486141	38.0	35.0	40.0	30.0	41.0
37	36.222956659644474	38.0	35.0	40.0	30.0	41.0
38	36.1553764172604	38.0	35.0	40.0	30.0	41.0
39	36.13486904395578	38.0	35.0	40.0	30.0	41.0
40	35.999983726655145	38.0	35.0	40.0	30.0	41.0
41	35.89670568324378	38.0	35.0	40.0	29.0	41.0
42	35.79757882216489	38.0	35.0	40.0	29.0	41.0
43	35.727041269202545	38.0	35.0	40.0	28.0	41.0
44	35.57768155135276	38.0	35.0	40.0	28.0	41.0
45	35.423018202475916	38.0	34.0	40.0	27.0	41.0
46	35.2561838710441	38.0	34.0	40.0	26.0	41.0
47	35.1513938859564	38.0	34.0	40.0	26.0	41.0
48	35.034036440457314	38.0	34.0	40.0	26.0	41.0
49	34.885836568277035	37.0	34.0	40.0	26.0	41.0
50	34.80584420195517	37.0	33.0	40.0	26.0	41.0
51	33.67814430610458	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	5.0
12	6.0
13	10.0
14	16.0
15	40.0
16	61.0
17	172.0
18	329.0
19	683.0
20	1207.0
21	1694.0
22	2388.0
23	3192.0
24	3934.0
25	4783.0
26	5706.0
27	6833.0
28	8371.0
29	10751.0
30	13997.0
31	18044.0
32	23338.0
33	30913.0
34	43938.0
35	66317.0
36	70444.0
37	93007.0
38	132611.0
39	133132.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.22658413615168	34.398596350036684	19.55375529623405	25.82106421757758
2	20.645992614860226	35.01594787795583	19.73897554855966	24.59908395862428
3	19.487774279830518	36.154046441167424	20.17835585958766	24.179823419414394
4	19.55257178024475	35.976075224276286	18.463589130589153	26.007763864889817
5	16.944398418822637	37.97636518569366	17.508343787724574	27.570892607759127
6	17.461003148152532	43.02258148507586	17.713979690865624	21.802435675905983
7	82.35510805500982	10.493348640140129	3.914331195114446	3.2372121097356024
8	83.72502781262575	9.967127843397163	2.9823123535398963	3.3255319904371907
9	77.03564750159775	14.348208156792197	5.475832603498473	3.140311738111582
10	28.110280019883067	48.43701919662934	11.695652945771297	11.757047837716287
11	17.030943025540275	33.24126565199896	33.74307643146259	15.984714890998179
12	21.572241815986935	28.752633323076193	32.08260349847327	17.592521362463607
13	22.04165384524345	30.959446824626603	28.26458091698819	18.73431841314176
14	18.536819182427156	32.755876156886885	28.928089568490073	19.779215092195894
15	15.865919473572088	37.23178568892466	27.974027741614787	18.928267095888465
16	18.51181740715317	35.94338059507184	26.602924468009565	18.941877529765428
17	17.547251875872842	34.71237602670012	28.305412218619075	19.434959878807962
18	18.52084171657159	33.885985987170685	28.60188297393898	18.991289322318742
19	18.758728430421094	37.37794991360333	26.431462589059578	17.431859066915994
20	19.566921911615026	35.96897412834048	27.209624352024996	17.254479608019505
21	19.562927545151133	34.69358771036997	28.171083153833408	17.57240159064549
22	19.06126470518617	33.22203351717282	27.055915212914528	20.66078656472649
23	18.634459251544488	35.54216867469879	26.512977252822683	19.310394820934032
24	15.897282647288566	36.402732738419296	28.50231969133903	19.19766492295311
25	17.88884417828485	36.38320472459583	26.615351385897224	19.112599711222096
26	19.615298127677704	37.008101166946766	24.555441806518807	18.821158898856723
27	16.874866854451202	35.99338414561981	26.751159845669513	20.380589154259475
28	17.4219471205056	35.89855492697706	28.413703931640118	18.26579402087722
29	19.061116765687505	35.46346486141027	26.267841503538712	19.207576869363503
30	18.63342367505385	35.16669822709304	26.819359954552986	19.380518143300115
31	19.041144933368052	36.31988661916822	24.889489194499017	19.749479252964708
32	19.445167704215685	34.4274445522759	25.796062442303597	20.33132530120482
33	19.116446138187328	34.509403034535	25.555512817478167	20.81863800979951
34	17.703475986460575	35.48743106019362	25.836006106942506	20.973086846403294
35	18.96628754704476	33.13770800293512	28.60587734040287	19.29012710961725
36	20.270965985750465	33.64632399933723	27.675781712310933	18.40692830260137
37	17.6744798447227	34.703647596279026	27.9383743224371	19.683498236561174
38	18.61212038724643	32.45526309560442	29.42028428054063	19.51233223660852
39	19.352261699055553	31.919278291949727	27.240395767746822	21.4880642412479
40	20.604865434232018	32.68575283452079	26.629701517267495	20.079680213979692
41	17.832775108291713	32.282469761166475	29.11271806282103	20.772037067720785
42	19.101356309323737	32.46621061850546	27.41481643667006	21.017616635500747
43	18.5742478755888	32.83384027268209	27.498254313915783	21.093657537813336
44	19.94786612067129	33.20871896229318	26.04933486401401	20.794080053021517
45	20.41357966246124	32.60897223471489	26.94658792340284	20.030860179421023
46	18.632092219565884	32.90766208251473	28.2494910881246	20.21075460979478
47	18.339172012213886	33.41938480838876	27.714393921461877	20.527049257935477
48	18.454416881672074	32.24370961251687	29.95301441522475	19.348859090586316
49	18.73816484010699	32.26619641631358	28.569632163230523	20.4260065803489
50	18.655022841858592	31.209760456363767	27.24379837621606	22.891418325561578
51	17.90911188960163	30.88281416431936	29.572366085165815	21.6357078609132
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23028.0
1	17536.0
2	12044.0
3	7376.0
4	2708.0
5	2387.5
6	2067.0
7	2076.0
8	2085.0
9	2426.5
10	2768.0
11	3256.5
12	3745.0
13	4342.0
14	4939.0
15	4891.0
16	4843.0
17	5173.5
18	5504.0
19	5326.5
20	5149.0
21	5264.5
22	5380.0
23	5679.0
24	5978.0
25	6621.5
26	8301.5
27	9338.0
28	10503.5
29	11669.0
30	15500.0
31	19331.0
32	23751.5
33	28172.0
34	26828.0
35	25484.0
36	27473.5
37	29463.0
38	29976.0
39	30489.0
40	33357.0
41	36225.0
42	37881.0
43	39537.0
44	41836.5
45	44136.0
46	48627.5
47	53119.0
48	55632.5
49	58146.0
50	54864.5
51	51583.0
52	45953.5
53	40324.0
54	35109.5
55	29895.0
56	25218.5
57	20542.0
58	19007.0
59	17472.0
60	15024.0
61	12576.0
62	10780.5
63	8985.0
64	8452.0
65	7919.0
66	6508.5
67	5098.0
68	3968.0
69	2838.0
70	2475.5
71	2113.0
72	2014.0
73	1915.0
74	1483.0
75	741.0
76	431.0
77	318.0
78	205.0
79	145.5
80	86.0
81	92.5
82	99.0
83	131.5
84	164.0
85	89.0
86	14.0
87	12.0
88	10.0
89	10.0
90	10.0
91	7.0
92	4.0
93	3.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	675952.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.147275088540567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.59037103867962	19.76482225593206
2	10.057539654404062	5.259545121152164
3	3.5875651353113556	2.814151574731298
4	1.8735167312063612	1.9594942941534417
5	1.2441391394813655	1.6265424164219702
6	0.862336416021316	1.3528648493145305
7	0.6363631035370119	1.1647412787065772
8	0.5312259468113714	1.1112088772357875
9	0.44192283856479403	1.0399570225076136
>10	3.8959186696400327	22.163095021371024
>50	0.7816730327649598	14.423351220451458
>100	0.4893400286414788	21.29317467512752
>500	0.005199598887571793	0.9606000252497989
>1k	0.002310932838920797	2.0186347125983746
>5k	0.0	0.0
>10k+	5.777332097301992E-4	3.047816655046382
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20176	2.984827325017161	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4706	0.6962032807063223	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3910	0.5784434397708713	No Hit
CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT	2616	0.38700972850143206	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2131	0.315259071650058	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGT	933	0.13802755225223093	No Hit
TGAGAAGGGAAAAGCCAGCCCTTTGTATAGAAATTTTGCTTTTGTTTTTTT	926	0.13699197576159253	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCC	789	0.11672426444481265	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9587899732525386E-4	0.0	0.0	0.4547660188889152	0.0
2	2.9587899732525386E-4	0.0	0.0	0.6237129263616351	0.0
3	2.9587899732525386E-4	0.0	0.0	0.7997609297701612	0.0
4	2.9587899732525386E-4	0.0	0.0	1.7390288067791795	0.0
5	2.9587899732525386E-4	0.0	0.0	1.8831218784765782	0.0
6	2.9587899732525386E-4	0.0	0.0	2.7062572490354344	0.0
7	2.9587899732525386E-4	0.0	0.0	3.3105901010722656	0.0
8	2.9587899732525386E-4	0.0	0.0	3.7026297725282267	0.0
9	2.9587899732525386E-4	0.0	0.0	4.5697031741898835	0.0
10	2.9587899732525386E-4	0.0	0.0	5.818460482401117	0.0
11	2.9587899732525386E-4	0.0	0.0	7.131275593533268	0.0
12	2.9587899732525386E-4	0.0	0.0	7.763125192321349	0.0
13	2.9587899732525386E-4	0.0	0.0	7.996129902714985	0.0
14	2.9587899732525386E-4	0.0	0.0	8.24747911094279	0.0
15	2.9587899732525386E-4	0.0	0.0	8.485809643288281	0.0
16	2.9587899732525386E-4	0.0	0.0	8.970903259403034	0.0
17	2.9587899732525386E-4	0.0	0.0	9.642104764835373	0.0
18	4.4381849598788077E-4	0.0	0.0	10.3869801406017	0.0
19	4.4381849598788077E-4	0.0	0.0	10.767332591663314	0.0
20	4.4381849598788077E-4	0.0	0.0	11.110848107557933	0.0
21	4.4381849598788077E-4	0.0	0.0	11.522563732336025	0.0
22	4.4381849598788077E-4	0.0	0.0	11.995378370061779	0.0
23	4.4381849598788077E-4	0.0	0.0	12.446150022486803	0.0
24	4.4381849598788077E-4	0.0	0.0	12.769249887565982	0.0
25	4.4381849598788077E-4	0.0	0.0	13.055956635974152	0.0
26	4.4381849598788077E-4	0.0	0.0	13.328609132009374	0.0
27	4.4381849598788077E-4	0.0	0.0	13.641944990176817	0.0
28	4.4381849598788077E-4	0.0	0.0	13.933089923544868	0.0
29	4.4381849598788077E-4	0.0	0.0	14.27172343598362	0.0
30	4.4381849598788077E-4	0.0	0.0	14.685066395247	0.0
31	4.4381849598788077E-4	0.0	0.0	15.006538925840887	0.0
32	4.4381849598788077E-4	0.0	0.0	15.310110777096599	0.0
33	4.4381849598788077E-4	0.0	0.0	15.628032759722585	0.0
34	4.4381849598788077E-4	0.0	0.0	15.93589485643951	0.0
35	4.4381849598788077E-4	0.0	0.0	16.2743804293796	0.0
36	4.4381849598788077E-4	0.0	0.0	16.615824792292944	0.0
37	4.4381849598788077E-4	0.0	0.0	16.926201860487136	0.0
38	4.4381849598788077E-4	0.0	0.0	17.265279191421875	0.0
39	4.4381849598788077E-4	0.0	0.0	17.586307903519778	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	45	3.8380676E-10	45.0	1
TCACGCG	35	1.2109376E-7	45.0	1
AACCGGT	20	7.0312223E-4	45.0	44
ACTATCG	25	3.8890947E-5	45.0	39
GATACGC	35	1.2109376E-7	45.0	15
GTCGCGG	20	7.0312223E-4	45.0	2
TCGTTAC	35	1.2109376E-7	45.0	30
AGATCGT	40	6.8084773E-9	45.0	34
CTACTCG	20	7.0312223E-4	45.0	1
TTTACGA	20	7.0312223E-4	45.0	23
CGAAAGC	20	7.0312223E-4	45.0	26
ACTGACG	55	1.8189894E-12	45.0	1
ACGTTAG	20	7.0312223E-4	45.0	31
CTATCCG	45	3.8380676E-10	45.0	1
AACCCGT	25	3.8890947E-5	45.0	10
CGGCCGT	20	7.0312223E-4	45.0	41
GCGCGCC	45	3.8380676E-10	45.0	16
CCGGCAT	20	7.0312223E-4	45.0	29
CATGACG	25	3.8890947E-5	45.0	1
GACGTCA	20	7.0312223E-4	45.0	13
>>END_MODULE