FastQCFastQC Report
Sat 18 Jun 2016
SRR3553463_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553463_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670477
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA205373.063043176723437No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47790.7127761280401863No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37920.5655674989596958No Hit
CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT23880.35616434269930214No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20780.3099286030691582No Hit
TGAGAAGGGAAAAGCCAGCCCTTTGTATAGAAATTTTGCTTTTGTTTTTTT9080.13542597285216346No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGT8180.12200269360470233No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCC7170.10693879133810705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAG302.1641426E-645.0000041
CGAGTGG302.1641426E-645.0000042
CCGATAT302.1641426E-645.00000433
CGACATA302.1641426E-645.00000414
CCGTCAA302.1641426E-645.00000413
ATCCGAT302.1641426E-645.00000431
ATATCCG302.1641426E-645.0000041
TCATGCG302.1641426E-645.0000041
CGTACTC302.1641426E-645.00000445
CATAACG302.1641426E-645.0000041
CATCGCG302.1641426E-645.0000041
CGCGTAG302.1641426E-645.0000041
CTTACGA302.1641426E-645.00000414
ACGCGAG207.031181E-445.01
TATTTCG207.031181E-445.017
CCGGGTA1050.045.05
CGAACCG207.031181E-445.01
GTATTCG207.031181E-445.031
ATTAGCG406.8084773E-945.01
GGTCGTT207.031181E-445.07