##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553463_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	670477
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58910149043144	31.0	31.0	34.0	30.0	34.0
2	31.789114317120497	31.0	31.0	34.0	30.0	34.0
3	31.97256281721819	33.0	31.0	34.0	30.0	34.0
4	35.72472732099684	37.0	35.0	37.0	33.0	37.0
5	35.47564345980548	37.0	35.0	37.0	33.0	37.0
6	32.71636909245209	37.0	35.0	37.0	26.0	37.0
7	34.272764017259355	37.0	35.0	37.0	28.0	37.0
8	35.37519706119673	37.0	35.0	37.0	32.0	37.0
9	37.22198822629262	39.0	37.0	39.0	34.0	39.0
10	36.62962637047952	38.0	35.0	39.0	32.0	39.0
11	36.70353494601604	39.0	37.0	39.0	32.0	39.0
12	36.82159566994841	39.0	37.0	39.0	32.0	39.0
13	36.841705233736576	39.0	37.0	39.0	33.0	39.0
14	37.99936910587537	40.0	37.0	41.0	33.0	41.0
15	37.997210940867475	40.0	37.0	41.0	33.0	41.0
16	37.91754228705832	40.0	37.0	41.0	33.0	41.0
17	37.912028302238554	39.0	37.0	41.0	33.0	41.0
18	37.73248299345093	39.0	37.0	41.0	33.0	41.0
19	37.65854757135592	39.0	36.0	41.0	33.0	41.0
20	37.549200047130626	39.0	36.0	41.0	32.0	41.0
21	37.505851804014156	39.0	36.0	41.0	32.0	41.0
22	37.45271351589987	39.0	36.0	41.0	32.0	41.0
23	37.28358765475922	39.0	35.0	40.0	32.0	41.0
24	37.18103081835768	39.0	35.0	40.0	32.0	41.0
25	37.17686960179096	39.0	35.0	40.0	32.0	41.0
26	37.10386187743949	39.0	35.0	40.0	32.0	41.0
27	37.0286497523405	39.0	35.0	40.0	31.0	41.0
28	36.97497900748273	39.0	35.0	40.0	31.0	41.0
29	36.8227813929486	39.0	35.0	40.0	31.0	41.0
30	36.78708591047866	39.0	35.0	40.0	31.0	41.0
31	36.42276617989879	39.0	35.0	40.0	30.0	41.0
32	36.50605315320287	39.0	35.0	40.0	30.0	41.0
33	36.481889759081966	39.0	35.0	40.0	30.0	41.0
34	36.47670986476792	39.0	35.0	40.0	30.0	41.0
35	36.40984254493443	39.0	35.0	40.0	30.0	41.0
36	36.357084583065486	39.0	35.0	40.0	30.0	41.0
37	36.26368391458618	38.0	35.0	40.0	30.0	41.0
38	36.18322627025237	38.0	35.0	40.0	30.0	41.0
39	36.13813300083373	38.0	35.0	40.0	30.0	41.0
40	36.027431216879926	38.0	35.0	40.0	30.0	41.0
41	35.99357770661783	38.0	35.0	40.0	30.0	41.0
42	35.87237295239061	38.0	35.0	40.0	29.0	41.0
43	35.82477698713006	38.0	35.0	40.0	29.0	41.0
44	35.62037623960255	38.0	35.0	40.0	28.0	41.0
45	35.46406215276587	38.0	34.0	40.0	27.0	41.0
46	35.18949792461188	38.0	34.0	40.0	26.0	41.0
47	35.15195599550768	38.0	34.0	40.0	26.0	41.0
48	35.157358119667045	38.0	34.0	40.0	26.0	41.0
49	35.04897856302304	38.0	34.0	40.0	26.0	41.0
50	34.95318855083769	38.0	34.0	40.0	26.0	41.0
51	33.89631560814166	36.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	6.0
12	6.0
13	13.0
14	16.0
15	34.0
16	77.0
17	190.0
18	342.0
19	664.0
20	1114.0
21	1713.0
22	2426.0
23	3188.0
24	4030.0
25	4792.0
26	5477.0
27	6581.0
28	8210.0
29	10468.0
30	13362.0
31	17784.0
32	23452.0
33	31231.0
34	44871.0
35	66246.0
36	71319.0
37	94795.0
38	129073.0
39	128975.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.060941687783473	34.4460734670988	19.59873343902923	25.894251406088504
2	20.72867525657107	35.06533408304834	19.62125471865552	24.584735941725068
3	19.401262086544353	36.163358325490655	20.33969845348908	24.095681134475903
4	19.526993468828906	36.020773270373176	18.371994863358474	26.080238397439437
5	16.849944144243576	38.17610447487386	17.427145151884403	27.54680622899816
6	16.81176237216191	45.324597264335694	16.983431198982217	20.880209164520185
7	82.38761359450064	10.569490079450897	3.831003897225408	3.211892428823062
8	83.54932383959479	10.122345732963248	2.9396981551939887	3.388632272247967
9	77.0053260589103	14.536516539717246	5.3391838944512635	3.118973506921192
10	28.022586904547058	48.55379080863326	11.720014258505513	11.70360802831417
11	16.91885031104721	33.47840418090404	33.6588727130088	15.943872795039947
12	21.46098971329367	28.856470840908788	32.17187166748449	17.510667778313053
13	21.957352750355344	31.122916968069003	28.184411993252567	18.73531828832309
14	18.444033128653185	32.928646321946914	28.77518542768805	19.852135121711857
15	15.799050526714565	37.45691500230433	27.873290209805855	18.870744261175254
16	18.419871226007754	36.15426032511183	26.52902336694622	18.896845081934206
17	17.416704823580822	34.84683292640911	28.326698753275654	19.409763496734413
18	18.578265921127795	34.21728112970318	28.360704394035892	18.843748555133136
19	18.736958911342224	37.564897826472794	26.314400046534036	17.383743215650945
20	19.48761851636969	36.00451618772904	27.11442748968272	17.393437806218557
21	19.497909697126076	34.73541970865518	28.264653373642943	17.502017220575798
22	19.12817292763212	33.341635880127136	26.99063502551169	20.53955616672906
23	18.69892628680775	35.53723692237019	26.618511895262625	19.14532489555943
24	15.85244534861002	36.69462188859573	28.354440197053737	19.09849256574051
25	17.912918713095305	36.42436653308018	26.629996256396566	19.03271849742795
26	19.62856294846803	37.28285981472892	24.408741836036135	18.679835400766915
27	16.87157053858671	36.277754494188464	26.654158159041998	20.19651680818283
28	17.271733407708243	36.14307425907227	28.305967244215687	18.2792250890038
29	19.033911677805506	35.67564584616624	26.259364601619446	19.031077874408815
30	18.54217221470684	35.41419019593513	26.810166493406935	19.233471095951092
31	19.087455647248152	36.45673155082128	24.875126216111813	19.580686585818754
32	19.369195363897642	34.569418488628244	25.764791335124098	20.296594812350012
33	19.01705800497258	34.81983722036699	25.532121161501436	20.63098361315899
34	17.604481585498085	35.77960168656046	25.855920486459638	20.759996241481808
35	18.97216459326718	33.35476086428021	28.50940449858832	19.163670043864293
36	20.23768153120838	33.817267408128835	27.649867184109223	18.29518387655356
37	17.591207453797818	34.99210263737608	27.743680991294255	19.67300891753185
38	18.4610359490333	32.59067798000528	29.57178247725127	19.37650359371015
39	19.30521106615141	32.08909477879182	27.123227194967164	21.482466960089607
40	20.51792977238593	32.907467370245364	26.688760389991007	19.885842467377703
41	17.76511349382604	32.59127457019406	28.936264778657584	20.707347157322324
42	19.080893155171616	32.68479008228471	27.371707008592388	20.862609753951293
43	18.5655883796163	32.98949852120207	27.475066258797842	20.969846840383784
44	19.973093782486202	33.334924240503405	26.012227115918968	20.679754861091432
45	20.238576416491544	32.85019471212286	26.793760263215592	20.117468608170004
46	18.589451987167347	33.184881808026226	28.213197469861008	20.01246873494542
47	18.336199452031913	33.74612402811729	27.497438390876944	20.42023812897385
48	18.386611322983487	32.53086981358048	29.824140127103536	19.25837873633249
49	18.688336810956976	32.54131014188406	28.511045121607452	20.25930792555151
50	18.657910711329396	31.45104157189583	27.269391791217295	22.621655925557477
51	17.80985775798424	31.10621244278327	29.461562439874893	21.622367359357593
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23369.0
1	17735.5
2	12102.0
3	7385.0
4	2668.0
5	2313.0
6	1958.0
7	2027.0
8	2096.0
9	2466.0
10	2836.0
11	3353.5
12	3871.0
13	4448.0
14	5025.0
15	4930.5
16	4836.0
17	5142.5
18	5449.0
19	5373.5
20	5298.0
21	5309.5
22	5321.0
23	5728.5
24	6136.0
25	6732.5
26	8382.5
27	9436.0
28	10623.5
29	11811.0
30	15668.5
31	19526.0
32	23694.0
33	27862.0
34	26869.5
35	25877.0
36	27681.0
37	29485.0
38	29949.0
39	30413.0
40	33261.0
41	36109.0
42	37646.5
43	39184.0
44	41444.5
45	43705.0
46	48237.0
47	52769.0
48	54846.5
49	56924.0
50	53651.5
51	50379.0
52	45053.5
53	39728.0
54	34465.0
55	29202.0
56	24811.0
57	20420.0
58	18700.5
59	16981.0
60	14586.5
61	12192.0
62	10428.0
63	8664.0
64	8257.0
65	7850.0
66	6429.5
67	5009.0
68	3880.5
69	2752.0
70	2376.0
71	2000.0
72	1901.0
73	1802.0
74	1432.0
75	761.0
76	460.0
77	340.5
78	221.0
79	149.5
80	78.0
81	89.0
82	100.0
83	116.0
84	132.0
85	75.5
86	19.0
87	16.5
88	14.0
89	11.5
90	9.0
91	6.5
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	670477.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.42637200160817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8327943368523	19.77559261065496
2	10.049946530189304	5.311672512061075
3	3.827049681325774	3.034051156420527
4	2.0170001628653216	2.13207986524733
5	1.2915554684445485	1.706556263491347
6	0.9786517975382701	1.551732987707331
7	0.6848641818800918	1.266893294865824
8	0.5765693578218701	1.2189309067623255
9	0.46883607007428196	1.115067275599951
>10	4.059238972422186	22.964367843474253
>50	0.7546430212791102	14.130280780700192
>100	0.45084056161329694	19.809426564738583
>500	0.005149194231623949	0.9071634818228629
>1k	0.0022885307696106443	1.9711150520874439
>5k	0.0	0.0
>10k+	5.721326924026611E-4	3.1050694043660223
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20537	3.063043176723437	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4779	0.7127761280401863	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3792	0.5655674989596958	No Hit
CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT	2388	0.35616434269930214	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2078	0.3099286030691582	No Hit
TGAGAAGGGAAAAGCCAGCCCTTTGTATAGAAATTTTGCTTTTGTTTTTTT	908	0.13542597285216346	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGT	818	0.12200269360470233	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCC	717	0.10693879133810705	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4914754719401262E-4	0.0	0.0	0.4217892634646677	0.0
2	1.4914754719401262E-4	0.0	0.0	0.5819737291510373	0.0
3	1.4914754719401262E-4	0.0	0.0	0.7612490808782404	0.0
4	1.4914754719401262E-4	0.0	0.0	1.654344593475988	0.0
5	1.4914754719401262E-4	0.0	0.0	1.7914111893472855	0.0
6	1.4914754719401262E-4	0.0	0.0	2.552511122678332	0.0
7	1.4914754719401262E-4	0.0	0.0	3.1534265903230088	0.0
8	1.4914754719401262E-4	0.0	0.0	3.532261360195801	0.0
9	1.4914754719401262E-4	0.0	0.0	4.3759890346723305	0.0
10	1.4914754719401262E-4	0.0	0.0	5.579758888075206	0.0
11	1.4914754719401262E-4	0.0	0.0	6.866305630170759	0.0
12	1.4914754719401262E-4	0.0	0.0	7.4584213925309895	0.0
13	1.4914754719401262E-4	0.0	0.0	7.689003500492933	0.0
14	1.4914754719401262E-4	0.0	0.0	7.938378199401321	0.0
15	1.4914754719401262E-4	0.0	0.0	8.176268537175773	0.0
16	1.4914754719401262E-4	0.0	0.0	8.632063441400675	0.0
17	1.4914754719401262E-4	0.0	0.0	9.265493074333646	0.0
18	1.4914754719401262E-4	0.0	0.0	10.015257794077948	0.0
19	1.4914754719401262E-4	0.0	0.0	10.363666464323162	0.0
20	1.4914754719401262E-4	0.0	0.0	10.709092183624493	0.0
21	1.4914754719401262E-4	0.0	0.0	11.105078921424598	0.0
22	1.4914754719401262E-4	0.0	0.0	11.567436317726036	0.0
23	1.4914754719401262E-4	0.0	0.0	12.008316467231538	0.0
24	1.4914754719401262E-4	0.0	0.0	12.330922611812188	0.0
25	1.4914754719401262E-4	0.0	0.0	12.596554393364723	0.0
26	1.4914754719401262E-4	0.0	0.0	12.860992994539709	0.0
27	1.4914754719401262E-4	0.0	0.0	13.160481269305286	0.0
28	1.4914754719401262E-4	0.0	0.0	13.452661314258357	0.0
29	1.4914754719401262E-4	0.0	0.0	13.78063677053799	0.0
30	1.4914754719401262E-4	0.0	0.0	14.151865015503887	0.0
31	2.9829509438802524E-4	0.0	0.0	14.469996733668717	0.0
32	2.9829509438802524E-4	0.0	0.0	14.775450910322053	0.0
33	4.4744264158203787E-4	0.0	0.0	15.082098267352944	0.0
34	4.4744264158203787E-4	0.0	0.0	15.385166083251178	0.0
35	4.4744264158203787E-4	0.0	0.0	15.728354589344601	0.0
36	4.4744264158203787E-4	0.0	0.0	16.04052040562167	0.0
37	4.4744264158203787E-4	0.0	0.0	16.33792061472653	0.0
38	4.4744264158203787E-4	0.0	0.0	16.650981316286764	0.0
39	4.4744264158203787E-4	0.0	0.0	16.96463860803577	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAG	30	2.1641426E-6	45.000004	1
CGAGTGG	30	2.1641426E-6	45.000004	2
CCGATAT	30	2.1641426E-6	45.000004	33
CGACATA	30	2.1641426E-6	45.000004	14
CCGTCAA	30	2.1641426E-6	45.000004	13
ATCCGAT	30	2.1641426E-6	45.000004	31
ATATCCG	30	2.1641426E-6	45.000004	1
TCATGCG	30	2.1641426E-6	45.000004	1
CGTACTC	30	2.1641426E-6	45.000004	45
CATAACG	30	2.1641426E-6	45.000004	1
CATCGCG	30	2.1641426E-6	45.000004	1
CGCGTAG	30	2.1641426E-6	45.000004	1
CTTACGA	30	2.1641426E-6	45.000004	14
ACGCGAG	20	7.031181E-4	45.0	1
TATTTCG	20	7.031181E-4	45.0	17
CCGGGTA	105	0.0	45.0	5
CGAACCG	20	7.031181E-4	45.0	1
GTATTCG	20	7.031181E-4	45.0	31
ATTAGCG	40	6.8084773E-9	45.0	1
GGTCGTT	20	7.031181E-4	45.0	7
>>END_MODULE