SRR3553459_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553459_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	494761
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15589	3.150814231517844	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3374	0.6819454241542886	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2763	0.5584514543385594	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCC	2472	0.4996351773886786	No Hit
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	2260	0.456786205865054	RNA PCR Primer, Index 30 (96% over 25bp)
AATGATACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGT	2260	0.456786205865054	No Hit
AATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCT	1726	0.34885530589516955	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1429	0.2888263222040541	No Hit
AATGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTT	598	0.12086643854305411	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	514	0.10388854416576891	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	25	3.887573E-5	45.000004	23
TCGTTCA	25	3.887573E-5	45.000004	16
CGAACAG	30	2.1629858E-6	45.000004	1
CGAAACT	30	2.1629858E-6	45.000004	29
CGGCCAC	35	1.2100463E-7	45.000004	29
CGTTGTC	30	2.1629858E-6	45.000004	45
TAACCTC	25	3.887573E-5	45.000004	30
CTACGGC	35	1.2100463E-7	45.000004	26
GGGCGAA	25	3.887573E-5	45.000004	7
TCTAACG	30	2.1629858E-6	45.000004	1
TCTCGAG	35	1.2100463E-7	45.000004	20
ACGTGAC	25	3.887573E-5	45.000004	41
ACTACCG	35	1.2100463E-7	45.000004	1
CGGGACG	50	2.1827873E-11	45.000004	6
CTCGAGA	35	1.2100463E-7	45.000004	21
TACTAGT	30	2.1629858E-6	45.000004	12
CACGTAG	25	3.887573E-5	45.000004	1
TAGGACG	25	3.887573E-5	45.000004	1
ATACGAG	25	3.887573E-5	45.000004	1
TTCGATG	30	2.1629858E-6	45.000004	1