##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553459_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	494761
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.531022049029733	31.0	31.0	34.0	30.0	34.0
2	31.73690327248914	31.0	31.0	34.0	30.0	34.0
3	31.91428386635163	33.0	31.0	34.0	30.0	34.0
4	35.66136781193344	37.0	35.0	37.0	33.0	37.0
5	35.426446304377265	37.0	35.0	37.0	33.0	37.0
6	32.70408540689343	37.0	35.0	37.0	26.0	37.0
7	34.20978209681038	37.0	35.0	37.0	28.0	37.0
8	35.31559480233891	37.0	35.0	37.0	32.0	37.0
9	37.175905133993986	39.0	37.0	39.0	34.0	39.0
10	36.57388112644287	38.0	35.0	39.0	32.0	39.0
11	36.65949215884033	39.0	35.0	39.0	32.0	39.0
12	36.70755172699546	39.0	35.0	39.0	32.0	39.0
13	36.74101030598612	39.0	37.0	39.0	32.0	39.0
14	37.84766180034401	39.0	37.0	41.0	33.0	41.0
15	37.905105697498385	40.0	37.0	41.0	33.0	41.0
16	37.80012571726551	39.0	37.0	41.0	33.0	41.0
17	37.77907312823768	39.0	37.0	41.0	33.0	41.0
18	37.57208429928794	39.0	36.0	41.0	33.0	41.0
19	37.53343331426689	39.0	36.0	41.0	32.0	41.0
20	37.44535644482892	39.0	36.0	41.0	32.0	41.0
21	37.403332113889334	39.0	36.0	40.0	32.0	41.0
22	37.354726827700645	39.0	36.0	40.0	32.0	41.0
23	37.17474295670031	39.0	35.0	40.0	32.0	41.0
24	37.05992590361811	39.0	35.0	40.0	31.0	41.0
25	37.062895013956236	39.0	35.0	40.0	32.0	41.0
26	37.00360780255517	39.0	35.0	40.0	31.0	41.0
27	36.94011047758413	39.0	35.0	40.0	31.0	41.0
28	36.87410891319243	39.0	35.0	40.0	31.0	41.0
29	36.70054228203112	39.0	35.0	40.0	31.0	41.0
30	36.671504019112255	39.0	35.0	40.0	31.0	41.0
31	36.24481921574255	38.0	35.0	40.0	30.0	41.0
32	36.35500372907323	38.0	35.0	40.0	30.0	41.0
33	36.24945377667197	38.0	35.0	40.0	30.0	41.0
34	36.25926457420856	38.0	35.0	40.0	30.0	41.0
35	36.195369077190804	38.0	35.0	40.0	30.0	41.0
36	36.153480165170656	38.0	35.0	40.0	30.0	41.0
37	36.14895474784795	38.0	35.0	40.0	30.0	41.0
38	35.995947538306375	38.0	35.0	40.0	30.0	41.0
39	35.85277133808041	38.0	35.0	40.0	29.0	41.0
40	35.675940504607276	38.0	35.0	40.0	28.0	41.0
41	35.706009568256185	38.0	35.0	40.0	29.0	41.0
42	35.65107395287826	38.0	35.0	40.0	28.0	41.0
43	35.63206881706521	38.0	35.0	40.0	28.0	41.0
44	35.48205092964078	38.0	35.0	40.0	27.0	41.0
45	35.265204816062706	38.0	34.0	40.0	26.0	41.0
46	34.91730956967101	38.0	34.0	40.0	25.0	41.0
47	34.89671174567114	38.0	33.0	40.0	25.0	41.0
48	34.92650997148118	37.0	34.0	40.0	26.0	41.0
49	34.85303207002977	37.0	34.0	40.0	26.0	41.0
50	34.720465032611706	37.0	33.0	40.0	25.0	41.0
51	33.60658580607606	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	6.0
12	6.0
13	9.0
14	11.0
15	24.0
16	56.0
17	115.0
18	256.0
19	479.0
20	842.0
21	1343.0
22	1926.0
23	2398.0
24	3088.0
25	3662.0
26	4379.0
27	5061.0
28	6484.0
29	8392.0
30	10685.0
31	13858.0
32	18535.0
33	24960.0
34	34811.0
35	50303.0
36	53953.0
37	69192.0
38	91092.0
39	88819.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.162181336039016	36.60939322218202	21.892792681719055	22.335632760059905
2	22.27964613217291	36.33451302750217	19.32225862588199	22.063582214442935
3	20.68938335883386	35.1737505583504	20.881193141739143	23.2556729410766
4	19.90132609482154	35.73240413047916	18.39312314430604	25.973146630393263
5	16.40569891321264	39.36567352721819	17.344333930928265	26.884293628640897
6	16.65551650190698	45.45709948843987	17.99778074666354	19.889603262989606
7	79.54871139802854	12.490475199136553	4.502375894623869	3.4584375082110355
8	82.00808066925242	9.795234466742528	3.295732687095385	4.90095217690966
9	76.29117897328204	14.338438154987966	5.835342721030963	3.5350401506990243
10	29.675136075802257	47.33780552630462	11.253716440867409	11.733341957025715
11	17.607289175985983	32.014447379643904	31.966747581155346	18.411515863214763
12	21.612657424493847	28.964287807648542	32.4479900396353	16.975064728222314
13	22.391619387946907	29.893019053644082	28.832709126224582	18.88265243218443
14	17.215787016357393	32.56218659110156	30.227119760854233	19.994906631686817
15	16.44450552893215	36.36745822730571	27.530464203928766	19.657572039833372
16	18.984317680657934	35.066628129541336	28.005643128702545	17.943411061098185
17	17.819108620121636	34.57346072143924	28.739128589359307	18.868302069079817
18	17.30269766614588	34.11808934010563	28.44363238007846	20.135580613670033
19	17.954325421769298	36.91196355412007	27.54319762471173	17.5905133993989
20	18.971180024294558	36.21041270431582	27.643650166444	17.17475710494562
21	19.217157374974988	35.71906435632558	27.676797484037746	17.386980784661684
22	19.35621441463656	33.29870381861141	27.20909691750158	20.135984849250445
23	17.678434638138416	35.18688821471377	26.389105042636746	20.74557210451107
24	15.1760142776007	38.085055208474394	27.18726819615936	19.551662317765548
25	17.049241957227835	36.70216528788647	26.074811878866765	20.173780876018927
26	18.40605868287921	36.47720818738745	25.354261956783176	19.76247117295017
27	16.17265710110538	35.410026255100945	26.691473256784587	21.725843387009082
28	17.67479651791471	36.26720780336365	27.36796150060332	18.690034178118324
29	17.8003116656325	35.44883287082045	27.397470697973365	19.35338476557368
30	18.58594351616235	36.45012440349987	24.41279729000467	20.551134790333112
31	20.44643777500652	36.772502278878086	23.810688392981664	18.970371553133734
32	19.111449770697366	35.050458706324875	24.874838558415075	20.963252964562688
33	18.276096943776896	36.499845379890495	24.83097899794042	20.393078678392193
34	17.709156542249694	35.34231679538201	26.281982613827687	20.666544048540608
35	17.930677640315224	34.69634833788435	25.864205141472347	21.508768880328077
36	19.181382526108564	35.73907401755595	26.809914281845177	18.26962917449031
37	18.740361507879562	35.78596534488369	24.071420342347114	21.402252804889635
38	19.943972948554958	35.285319578543984	25.986284286756632	18.784423186144423
39	19.796831197285155	34.95202734249466	24.917081176568082	20.334060283652107
40	18.980881678224435	37.04091470427136	23.35915724966196	20.61904636784225
41	17.74573986227694	34.489379720713636	26.90450540766148	20.860375009347948
42	18.253257633483642	34.343652793975274	25.27220213395963	22.130887438581457
43	17.25701904555937	33.90930166282306	26.637709924589853	22.195969367027715
44	19.052027140376868	32.58644072592626	25.79811262407506	22.563419509621816
45	20.80337779250992	33.817135950489224	25.752434003488556	19.627052253512304
46	17.876914308120487	34.38892717898136	28.66677042046564	19.06738809243251
47	17.983228265768723	35.56363577565734	25.71382950555925	20.739306453014688
48	17.343929695347853	33.258684496150664	29.2042016246228	20.19318418387868
49	18.668407574566306	32.880926346256075	26.892580458039333	21.558085621138286
50	19.232114091450214	31.795149577270642	25.751423414537523	23.221312916741617
51	17.358886411823082	31.624157926756556	28.523671024999945	22.493284636420412
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17579.0
1	13122.5
2	8666.0
3	5427.0
4	2188.0
5	1829.0
6	1470.0
7	1552.0
8	1634.0
9	1981.0
10	2328.0
11	2906.5
12	3485.0
13	3657.5
14	3830.0
15	4044.5
16	4259.0
17	4211.0
18	4163.0
19	4422.0
20	4681.0
21	4440.0
22	4199.0
23	4628.5
24	5058.0
25	5154.5
26	5735.0
27	6219.0
28	7312.5
29	8406.0
30	10423.5
31	12441.0
32	14331.5
33	16222.0
34	17892.0
35	19562.0
36	19964.0
37	20366.0
38	21319.0
39	22272.0
40	23678.0
41	25084.0
42	26574.5
43	28065.0
44	33142.0
45	38219.0
46	38823.0
47	39427.0
48	41073.5
49	42720.0
50	39786.5
51	36853.0
52	33445.0
53	30037.0
54	25681.0
55	21325.0
56	18256.0
57	15187.0
58	13377.5
59	11568.0
60	10094.5
61	8621.0
62	8248.0
63	7875.0
64	6621.5
65	5368.0
66	4244.0
67	3120.0
68	2858.5
69	2597.0
70	2050.5
71	1504.0
72	1391.0
73	1278.0
74	1100.5
75	578.5
76	234.0
77	269.0
78	304.0
79	187.5
80	71.0
81	64.5
82	58.0
83	43.5
84	29.0
85	17.5
86	6.0
87	6.0
88	6.0
89	4.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	494761.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.404672364808253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.88959002606508	22.315085306160636
2	9.279551985614273	5.457243716583871
3	3.502256912052936	3.08948151108905
4	1.9303825565043162	2.2704906645100147
5	1.3119733907493327	1.9289073853165342
6	0.9747641328428416	1.719757197552611
7	0.7445041098216597	1.5324329596491386
8	0.596432506022927	1.4030321941860553
9	0.49608106464016305	1.312839105491633
>10	4.327682832137313	26.690097401780164
>50	0.6671289373428978	13.600781057982156
>100	0.2726950400817685	11.93264495438997
>500	0.0013913012249069819	0.22746380713605288
>1k	0.004869554287174437	3.330953808816084
>5k	0.0	0.0
>10k+	6.956506124534909E-4	3.1887889293560514
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15589	3.150814231517844	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3374	0.6819454241542886	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2763	0.5584514543385594	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCC	2472	0.4996351773886786	No Hit
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	2260	0.456786205865054	RNA PCR Primer, Index 30 (96% over 25bp)
AATGATACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGT	2260	0.456786205865054	No Hit
AATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCT	1726	0.34885530589516955	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1429	0.2888263222040541	No Hit
AATGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTT	598	0.12086643854305411	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	514	0.10388854416576891	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5628980457230865	0.0
2	0.0	0.0	0.0	0.744804056908285	0.0
3	0.0	0.0	0.0	0.9766331622743102	0.0
4	0.0	0.0	0.0	2.202881795452754	0.0
5	0.0	0.0	0.0	2.378926390721985	0.0
6	0.0	0.0	0.0	3.1993225011672304	0.0
7	0.0	0.0	0.0	3.8501417856298294	0.0
8	0.0	0.0	0.0	4.224463933090926	0.0
9	0.0	0.0	0.0	5.456573982185338	0.0
10	0.0	0.0	0.0	6.531234272709449	0.0
11	0.0	0.0	0.0	8.17829214509632	0.0
12	0.0	0.0	0.0	8.77211421272089	0.0
13	0.0	0.0	0.0	9.036080046729634	0.0
14	0.0	0.0	0.0	9.383520528093362	0.0
15	0.0	0.0	0.0	9.644050359668608	0.0
16	0.0	0.0	0.0	10.12771823163103	0.0
17	0.0	0.0	0.0	10.815120836120874	0.0
18	0.0	0.0	0.0	11.693524752355177	0.0
19	0.0	0.0	0.0	12.094728565913643	0.0
20	0.0	0.0	0.0	12.48299684089894	0.0
21	0.0	0.0	0.0	12.89834889977181	0.0
22	2.0211779020577611E-4	0.0	0.0	13.384240067426495	0.0
23	2.0211779020577611E-4	0.0	0.0	13.844058040144635	0.0
24	2.0211779020577611E-4	0.0	0.0	14.184626516641368	0.0
25	2.0211779020577611E-4	0.0	0.0	14.450815646342376	0.0
26	2.0211779020577611E-4	0.0	0.0	14.735397494952108	0.0
27	2.0211779020577611E-4	0.0	0.0	15.019170872401018	0.0
28	2.0211779020577611E-4	0.0	0.0	15.294859538241697	0.0
29	2.0211779020577611E-4	0.0	0.0	15.623300947326083	0.0
30	2.0211779020577611E-4	0.0	0.0	15.996208270255739	0.0
31	2.0211779020577611E-4	0.0	0.0	16.305246371480372	0.0
32	2.0211779020577611E-4	0.0	0.0	16.59771081390813	0.0
33	2.0211779020577611E-4	0.0	0.0	16.908972210825024	0.0
34	2.0211779020577611E-4	0.0	0.0	17.217201840888833	0.0
35	2.0211779020577611E-4	0.0	0.0	17.566663500154622	0.0
36	2.0211779020577611E-4	0.0	0.0	17.884190548567894	0.0
37	2.0211779020577611E-4	0.0	0.0	18.192824414212115	0.0
38	2.0211779020577611E-4	0.0	0.0	18.49984133753469	0.0
39	2.0211779020577611E-4	0.0	0.0	18.82040015280105	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	25	3.887573E-5	45.000004	23
TCGTTCA	25	3.887573E-5	45.000004	16
CGAACAG	30	2.1629858E-6	45.000004	1
CGAAACT	30	2.1629858E-6	45.000004	29
CGGCCAC	35	1.2100463E-7	45.000004	29
CGTTGTC	30	2.1629858E-6	45.000004	45
TAACCTC	25	3.887573E-5	45.000004	30
CTACGGC	35	1.2100463E-7	45.000004	26
GGGCGAA	25	3.887573E-5	45.000004	7
TCTAACG	30	2.1629858E-6	45.000004	1
TCTCGAG	35	1.2100463E-7	45.000004	20
ACGTGAC	25	3.887573E-5	45.000004	41
ACTACCG	35	1.2100463E-7	45.000004	1
CGGGACG	50	2.1827873E-11	45.000004	6
CTCGAGA	35	1.2100463E-7	45.000004	21
TACTAGT	30	2.1629858E-6	45.000004	12
CACGTAG	25	3.887573E-5	45.000004	1
TAGGACG	25	3.887573E-5	45.000004	1
ATACGAG	25	3.887573E-5	45.000004	1
TTCGATG	30	2.1629858E-6	45.000004	1
>>END_MODULE