FastQCFastQC Report
Sat 18 Jun 2016
SRR3553458_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553458_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650570
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA196281.1891649551367103No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT55880.33854971312940374TruSeq Adapter, Index 16 (95% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44680.27069436618865No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35090.21259322537062952No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23020.13946697201572789No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG17050.10329764869105824No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16890.10232828659190463No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGC207.0341805E-445.00000435
ATAGCGC207.0341805E-445.00000421
TTCGTTC207.0341805E-445.00000432
ATTCGTT207.0341805E-445.00000423
ATTCGAT207.0341805E-445.00000442
TCGATAT207.0341805E-445.00000440
TATTACG406.8175723E-945.0000041
GTATCGA207.0341805E-445.0000049
CGCAATA406.8175723E-945.00000420
ATACGAT207.0341805E-445.00000434
GCGTAAT207.0341805E-445.0000041
TGCGTAA207.0341805E-445.00000415
TATCCGT207.0341805E-445.00000440
AACCGAT453.8562575E-1045.044
GTCGAAA351.2123564E-745.013
ACGCATA502.1827873E-1145.01
GCGAAGT253.891548E-545.010
CGCATAT551.8189894E-1245.02
TAGCGAA253.891548E-545.039
CGCTATA351.2123564E-745.030