Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553458_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1650570 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 19628 | 1.1891649551367103 | No Hit |
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT | 5588 | 0.33854971312940374 | TruSeq Adapter, Index 16 (95% over 23bp) |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 4468 | 0.27069436618865 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3509 | 0.21259322537062952 | No Hit |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2302 | 0.13946697201572789 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 1705 | 0.10329764869105824 | No Hit |
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 1689 | 0.10232828659190463 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACGTTGC | 20 | 7.0341805E-4 | 45.000004 | 35 |
ATAGCGC | 20 | 7.0341805E-4 | 45.000004 | 21 |
TTCGTTC | 20 | 7.0341805E-4 | 45.000004 | 32 |
ATTCGTT | 20 | 7.0341805E-4 | 45.000004 | 23 |
ATTCGAT | 20 | 7.0341805E-4 | 45.000004 | 42 |
TCGATAT | 20 | 7.0341805E-4 | 45.000004 | 40 |
TATTACG | 40 | 6.8175723E-9 | 45.000004 | 1 |
GTATCGA | 20 | 7.0341805E-4 | 45.000004 | 9 |
CGCAATA | 40 | 6.8175723E-9 | 45.000004 | 20 |
ATACGAT | 20 | 7.0341805E-4 | 45.000004 | 34 |
GCGTAAT | 20 | 7.0341805E-4 | 45.000004 | 1 |
TGCGTAA | 20 | 7.0341805E-4 | 45.000004 | 15 |
TATCCGT | 20 | 7.0341805E-4 | 45.000004 | 40 |
AACCGAT | 45 | 3.8562575E-10 | 45.0 | 44 |
GTCGAAA | 35 | 1.2123564E-7 | 45.0 | 13 |
ACGCATA | 50 | 2.1827873E-11 | 45.0 | 1 |
GCGAAGT | 25 | 3.891548E-5 | 45.0 | 10 |
CGCATAT | 55 | 1.8189894E-12 | 45.0 | 2 |
TAGCGAA | 25 | 3.891548E-5 | 45.0 | 39 |
CGCTATA | 35 | 1.2123564E-7 | 45.0 | 30 |