##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553458_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1650570
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.579056931847784	31.0	31.0	34.0	30.0	34.0
2	31.786704592958795	31.0	31.0	34.0	30.0	34.0
3	31.963429603106807	33.0	31.0	34.0	30.0	34.0
4	35.690823776028886	37.0	35.0	37.0	33.0	37.0
5	35.42912751352563	37.0	35.0	37.0	33.0	37.0
6	34.064200851826946	37.0	35.0	37.0	32.0	37.0
7	34.90532058622173	37.0	35.0	37.0	32.0	37.0
8	35.59327141532925	37.0	35.0	37.0	33.0	37.0
9	37.25411645673919	39.0	37.0	39.0	34.0	39.0
10	36.67959311025888	38.0	37.0	39.0	32.0	39.0
11	36.67204177950647	39.0	35.0	39.0	32.0	39.0
12	36.54398298769516	39.0	35.0	39.0	32.0	39.0
13	36.50265605215168	39.0	35.0	39.0	32.0	39.0
14	37.63787358306524	39.0	36.0	41.0	33.0	41.0
15	37.625008330455536	39.0	36.0	41.0	33.0	41.0
16	37.5341312395112	39.0	36.0	41.0	32.0	41.0
17	37.51784171528623	39.0	36.0	41.0	32.0	41.0
18	37.432142229654	39.0	36.0	41.0	32.0	41.0
19	37.41846392458363	39.0	36.0	41.0	32.0	41.0
20	37.310932586924515	39.0	35.0	41.0	32.0	41.0
21	37.26521747032843	39.0	35.0	40.0	32.0	41.0
22	37.24009099886706	39.0	35.0	40.0	32.0	41.0
23	37.11821734309966	39.0	35.0	40.0	32.0	41.0
24	36.9404575389108	39.0	35.0	40.0	31.0	41.0
25	36.98504334866137	39.0	35.0	40.0	31.0	41.0
26	36.934899458974776	39.0	35.0	40.0	31.0	41.0
27	36.9053848064608	39.0	35.0	40.0	31.0	41.0
28	36.78866876291221	39.0	35.0	40.0	31.0	41.0
29	36.63734103976202	39.0	35.0	40.0	31.0	41.0
30	36.65159914453795	39.0	35.0	40.0	31.0	41.0
31	36.56612442974245	39.0	35.0	40.0	30.0	41.0
32	36.47985180876909	39.0	35.0	40.0	30.0	41.0
33	36.4505946430627	39.0	35.0	40.0	30.0	41.0
34	36.39282490291233	38.0	35.0	40.0	30.0	41.0
35	36.278694026911914	38.0	35.0	40.0	30.0	41.0
36	36.23695632417892	38.0	35.0	40.0	30.0	41.0
37	36.138644831785385	38.0	35.0	40.0	30.0	41.0
38	36.05369660177999	38.0	35.0	40.0	30.0	41.0
39	36.02468844096282	38.0	35.0	40.0	30.0	41.0
40	35.88155909776623	38.0	35.0	40.0	29.0	41.0
41	35.800783365746376	38.0	35.0	40.0	29.0	41.0
42	35.69395481560915	38.0	34.0	40.0	29.0	41.0
43	35.58355780124442	38.0	34.0	40.0	28.0	41.0
44	35.46301701836335	38.0	34.0	40.0	28.0	41.0
45	35.28305615635811	38.0	34.0	40.0	27.0	41.0
46	35.12238620597733	37.0	34.0	40.0	27.0	41.0
47	35.0231489727791	37.0	34.0	40.0	27.0	41.0
48	34.89923541564429	37.0	34.0	40.0	26.0	41.0
49	34.72615581284041	37.0	33.0	40.0	26.0	41.0
50	34.63611964351709	37.0	33.0	40.0	26.0	41.0
51	33.40204777743446	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	14.0
11	27.0
12	31.0
13	54.0
14	77.0
15	151.0
16	278.0
17	514.0
18	946.0
19	1634.0
20	2509.0
21	3757.0
22	5506.0
23	7108.0
24	9201.0
25	11386.0
26	14441.0
27	17761.0
28	22239.0
29	28427.0
30	36847.0
31	48059.0
32	62387.0
33	84542.0
34	124753.0
35	152005.0
36	168818.0
37	229969.0
38	315710.0
39	301365.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.105375718691118	31.623318005295143	20.63820377203027	26.633102503983473
2	23.318550561321242	30.594582477568355	20.537571869111883	25.54929509199852
3	21.345292838231643	31.48451746972258	19.982369726821645	27.187819965224136
4	21.443864846689326	31.820340851948114	19.57529823030832	27.16049607105424
5	18.6580393439842	35.57437733631412	17.229381365225347	28.53820195447633
6	19.765656712529612	40.700121776113704	18.235336883622022	21.29888462773466
7	83.44365885724325	7.644450099056689	4.806884894309238	4.1050061493908165
8	85.02965642172097	6.644128997861346	4.453734164561333	3.872480415856341
9	77.45039592383237	10.328613751613078	7.580835711299732	4.640154613254815
10	36.61844090223377	33.071181470643474	13.75397589923481	16.55640172788794
11	27.845653319762263	29.688410670253308	22.900452570930042	19.565483439054386
12	27.509102915962362	25.234615920560778	27.749868227339647	19.506412936137213
13	25.311134941262715	25.459326172170826	25.93455594128089	23.294982945285568
14	21.626589602379784	28.20783123405854	27.97300326553857	22.192575898023108
15	21.517475781093804	29.676293644013885	27.516978983017985	21.289251591874322
16	22.919597472388325	28.518632957099673	25.96533318792902	22.596436382582986
17	22.458544623978383	29.089526648369958	26.16477943982988	22.287149287821784
18	23.669701981739642	27.976032522098425	26.135516821461678	22.218748674700254
19	23.16369496598145	31.467856558643376	24.84990033745918	20.518548137915992
20	24.629673385557716	29.268676881319788	25.593279897247616	20.50836983587488
21	25.45593340482379	28.34390543872723	25.662225776549917	20.537935379899064
22	24.047268519359978	27.327105181846274	25.750316557310505	22.875309741483246
23	23.293831827792825	28.15027535942129	24.705525969816488	23.850366842969397
24	21.209703314612526	28.537535518033163	26.721859721187226	23.530901446167082
25	22.178338392191787	29.815275934980036	24.81730553687514	23.189080135953034
26	22.883670489588447	27.738114711887405	25.467020483832858	23.91119431469129
27	21.17371574668145	28.844035696759303	24.790526908886022	25.191721647673226
28	21.25938312219415	29.621221759755723	26.930211987374058	22.189183130676067
29	22.84441132457272	29.243473466741793	25.119383001023888	22.792732207661594
30	23.026469643820015	28.788055035533176	24.630521577394475	23.55495374325233
31	23.811834699528042	29.100916653035014	24.408779997213085	22.678468650223863
32	23.80686671876988	27.532488776604445	25.34330564592838	23.317338858697298
33	22.361911339719008	27.616944449493207	25.0710360663286	24.950108144459186
34	20.444755448117924	28.661189770806452	27.111422114784588	23.78263266629104
35	21.962291814342922	27.321894860563322	27.306748577764044	23.40906474732971
36	24.030425852887184	29.59904760173758	25.042015788485187	21.328510756890044
37	22.4958044796646	30.318193109047176	24.540007391386006	22.645995019902216
38	21.855722568567224	28.85342639209485	26.226697443913316	23.06415359542461
39	21.837244103552106	26.495937766953237	26.71319604742604	24.95362208206862
40	20.90132499681928	26.660789908940547	27.27990936464373	25.157975729596444
41	20.435849433831947	26.505328462288784	29.388696026221243	23.670126077658022
42	21.819735000636147	27.14940899204517	26.937361032855318	24.09349497446337
43	21.874079863319945	26.090623239244625	27.89902882034691	24.13626807708852
44	22.142471994523106	26.289221299308725	25.981812343614628	25.58649436255354
45	22.687132323985047	27.449365976602024	26.272984484147898	23.59051721526503
46	21.007409561545405	26.781657245678765	28.099807945134103	24.111125247641723
47	21.01001472218688	27.18915283811047	27.863586518596605	23.937245921106044
48	20.958032679619766	26.78995740865277	28.636955718327606	23.615054193399857
49	21.421327177884002	27.302992299629825	27.616399183312428	23.65928133917374
50	20.535754315175968	26.460556050334127	28.00038774483966	25.003301889650242
51	21.633678062729842	25.15858158090841	28.393706416571245	24.814033939790498
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23093.0
1	17523.5
2	11954.0
3	7468.5
4	2983.0
5	2741.5
6	2500.0
7	2476.5
8	2453.0
9	2602.0
10	2751.0
11	3079.5
12	3408.0
13	3694.0
14	3980.0
15	4209.0
16	4438.0
17	4515.5
18	4593.0
19	4984.5
20	5376.0
21	5939.0
22	6502.0
23	7633.0
24	8764.0
25	9268.5
26	11501.0
27	13229.0
28	18201.0
29	23173.0
30	27491.5
31	31810.0
32	36551.5
33	41293.0
34	45385.5
35	49478.0
36	50013.5
37	50549.0
38	54382.0
39	58215.0
40	59972.0
41	61729.0
42	72950.0
43	84171.0
44	89687.5
45	95204.0
46	106012.0
47	116820.0
48	124236.5
49	131653.0
50	128555.5
51	125458.0
52	116394.0
53	107330.0
54	104112.5
55	100895.0
56	91465.0
57	82035.0
58	80903.5
59	79772.0
60	74760.0
61	69748.0
62	65157.0
63	60566.0
64	53777.5
65	46989.0
66	42183.0
67	37377.0
68	32725.5
69	28074.0
70	24622.5
71	21171.0
72	17661.5
73	14152.0
74	12773.5
75	9402.5
76	7410.0
77	5257.5
78	3105.0
79	3107.0
80	3109.0
81	2125.5
82	1142.0
83	809.5
84	477.0
85	343.0
86	209.0
87	150.0
88	91.0
89	97.5
90	104.0
91	73.0
92	42.0
93	30.0
94	18.0
95	11.5
96	5.0
97	4.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1650570.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.847325880106816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.00768046787991	15.845568841049221
2	9.99148898216068	4.165916536772009
3	4.022464578665885	2.5157288973790277
4	2.045148192109423	1.7054348333606573
5	1.2141129811872393	1.2655504487039184
6	0.8277484597720149	1.0353805132574208
7	0.5890583209529514	0.859620354550631
8	0.41776988471552673	0.6967507943647393
9	0.3351051206742711	0.6287441089310144
>10	2.543712852363256	11.790241377742976
>50	0.8517729541991416	13.007261563324205
>100	1.1402565064939467	42.391653833256335
>500	0.010706633863756164	1.4396702197767917
>1k	0.0023792519697235916	1.08905286005079
>5k	2.9740649621544894E-4	0.34646327252854053
>10k+	2.9740649621544894E-4	1.216961544951717
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19628	1.1891649551367103	No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	5588	0.33854971312940374	TruSeq Adapter, Index 16 (95% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4468	0.27069436618865	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3509	0.21259322537062952	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2302	0.13946697201572789	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1705	0.10329764869105824	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1689	0.10232828659190463	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.2409591837971124E-4	0.0	0.0	0.4102825084667721	0.0
2	4.2409591837971124E-4	0.0	0.0	0.5377536245054738	0.0
3	4.2409591837971124E-4	0.0	0.0	0.7004247017696917	0.0
4	4.2409591837971124E-4	0.0	0.0	1.4175708997497833	0.0
5	4.2409591837971124E-4	0.0	0.0	1.5302592437763924	0.0
6	4.2409591837971124E-4	0.0	0.0	2.230805115808478	0.0
7	4.2409591837971124E-4	0.0	0.0	2.8073938094112942	0.0
8	4.2409591837971124E-4	0.0	0.0	3.230641535954246	0.0
9	4.2409591837971124E-4	0.0	0.0	3.8748432359730276	0.0
10	4.2409591837971124E-4	0.0	0.0	4.903821104224601	0.0
11	4.2409591837971124E-4	0.0	0.0	5.769582629031183	0.0
12	4.2409591837971124E-4	0.0	0.0	6.211187650326857	0.0
13	4.8468104957681286E-4	0.0	0.0	6.387429796979226	0.0
14	4.8468104957681286E-4	0.0	0.0	6.590753497276698	0.0
15	4.8468104957681286E-4	0.0	0.0	6.782687192909116	0.0
16	5.452661807739145E-4	0.0	0.0	7.177096397002248	0.0
17	5.452661807739145E-4	0.0	0.0	7.64735818535415	0.0
18	5.452661807739145E-4	0.0	0.0	8.260419127937622	0.0
19	5.452661807739145E-4	0.0	0.0	8.520087000248399	0.0
20	6.058513119710161E-4	0.0	0.0	8.80871456527139	0.0
21	6.664364431681177E-4	0.0	0.0	9.11933453291893	0.0
22	6.664364431681177E-4	0.0	0.0	9.482481809314358	0.0
23	6.664364431681177E-4	0.0	0.0	9.83339088920797	0.0
24	6.664364431681177E-4	0.0	0.0	10.105175787758169	0.0
25	6.664364431681177E-4	0.0	0.0	10.347213386890589	0.0
26	6.664364431681177E-4	0.0	0.0	10.557746717800518	0.0
27	6.664364431681177E-4	0.0	0.0	10.807296873201379	0.0
28	6.664364431681177E-4	0.0	0.0	11.05266665454964	0.0
29	6.664364431681177E-4	0.0	0.0	11.306700109659088	0.0
30	6.664364431681177E-4	0.0	0.0	11.627013698298164	0.0
31	6.664364431681177E-4	0.0	0.0	11.873474012007973	0.0
32	6.664364431681177E-4	0.0	0.0	12.141199706767965	0.0
33	6.664364431681177E-4	0.0	0.0	12.427403866543074	0.0
34	6.664364431681177E-4	0.0	0.0	12.685920621361106	0.0
35	6.664364431681177E-4	0.0	0.0	12.97406350533452	0.0
36	6.664364431681177E-4	0.0	0.0	13.23118680213502	0.0
37	6.664364431681177E-4	0.0	0.0	13.492369302725725	0.0
38	6.664364431681177E-4	0.0	0.0	13.755672282908328	0.0
39	6.664364431681177E-4	0.0	0.0	14.051024797494199	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTGC	20	7.0341805E-4	45.000004	35
ATAGCGC	20	7.0341805E-4	45.000004	21
TTCGTTC	20	7.0341805E-4	45.000004	32
ATTCGTT	20	7.0341805E-4	45.000004	23
ATTCGAT	20	7.0341805E-4	45.000004	42
TCGATAT	20	7.0341805E-4	45.000004	40
TATTACG	40	6.8175723E-9	45.000004	1
GTATCGA	20	7.0341805E-4	45.000004	9
CGCAATA	40	6.8175723E-9	45.000004	20
ATACGAT	20	7.0341805E-4	45.000004	34
GCGTAAT	20	7.0341805E-4	45.000004	1
TGCGTAA	20	7.0341805E-4	45.000004	15
TATCCGT	20	7.0341805E-4	45.000004	40
AACCGAT	45	3.8562575E-10	45.0	44
GTCGAAA	35	1.2123564E-7	45.0	13
ACGCATA	50	2.1827873E-11	45.0	1
GCGAAGT	25	3.891548E-5	45.0	10
CGCATAT	55	1.8189894E-12	45.0	2
TAGCGAA	25	3.891548E-5	45.0	39
CGCTATA	35	1.2123564E-7	45.0	30
>>END_MODULE