Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553457_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1632522 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 19494 | 1.1941033566469548 | No Hit |
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT | 5162 | 0.3161978827850406 | TruSeq Adapter, Index 16 (95% over 23bp) |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 4251 | 0.2603946531807841 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3441 | 0.21077817021761422 | No Hit |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2402 | 0.14713431120683212 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 1722 | 0.10548096748466483 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCGATC | 20 | 7.034158E-4 | 45.000004 | 27 |
CGTATAC | 20 | 7.034158E-4 | 45.000004 | 22 |
ATTCGGA | 20 | 7.034158E-4 | 45.000004 | 27 |
TCGCTAT | 20 | 7.034158E-4 | 45.000004 | 15 |
GCGATAT | 20 | 7.034158E-4 | 45.000004 | 22 |
ACGTAAG | 40 | 6.8175723E-9 | 45.000004 | 1 |
TATCGGC | 40 | 6.8175723E-9 | 45.000004 | 40 |
ATCGCAT | 20 | 7.034158E-4 | 45.000004 | 43 |
AATCGTC | 20 | 7.034158E-4 | 45.000004 | 18 |
AGTCGAT | 20 | 7.034158E-4 | 45.000004 | 36 |
ACGCATA | 25 | 3.891529E-5 | 45.0 | 1 |
TGGACGT | 25 | 3.891529E-5 | 45.0 | 17 |
CGCATAT | 25 | 3.891529E-5 | 45.0 | 2 |
ATAGCGC | 35 | 1.2123564E-7 | 45.0 | 21 |
CGCGCAT | 25 | 3.891529E-5 | 45.0 | 12 |
CGTTCGA | 25 | 3.891529E-5 | 45.0 | 19 |
GTTAACG | 35 | 1.2123564E-7 | 45.0 | 1 |
ACTACGT | 25 | 3.891529E-5 | 45.0 | 30 |
ACTCGGT | 25 | 3.891529E-5 | 45.0 | 20 |
ACGTCGA | 25 | 3.891529E-5 | 45.0 | 35 |