FastQCFastQC Report
Sat 18 Jun 2016
SRR3553457_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553457_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632522
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA194941.1941033566469548No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT51620.3161978827850406TruSeq Adapter, Index 16 (95% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42510.2603946531807841No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34410.21077817021761422No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24020.14713431120683212No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG17220.10548096748466483No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC207.034158E-445.00000427
CGTATAC207.034158E-445.00000422
ATTCGGA207.034158E-445.00000427
TCGCTAT207.034158E-445.00000415
GCGATAT207.034158E-445.00000422
ACGTAAG406.8175723E-945.0000041
TATCGGC406.8175723E-945.00000440
ATCGCAT207.034158E-445.00000443
AATCGTC207.034158E-445.00000418
AGTCGAT207.034158E-445.00000436
ACGCATA253.891529E-545.01
TGGACGT253.891529E-545.017
CGCATAT253.891529E-545.02
ATAGCGC351.2123564E-745.021
CGCGCAT253.891529E-545.012
CGTTCGA253.891529E-545.019
GTTAACG351.2123564E-745.01
ACTACGT253.891529E-545.030
ACTCGGT253.891529E-545.020
ACGTCGA253.891529E-545.035