##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553457_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1632522
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.559658001546072	31.0	31.0	34.0	30.0	34.0
2	31.770537854926303	31.0	31.0	34.0	30.0	34.0
3	31.945054339237082	33.0	31.0	34.0	30.0	34.0
4	35.68285205344859	37.0	35.0	37.0	33.0	37.0
5	35.443024351279796	37.0	35.0	37.0	33.0	37.0
6	32.60917831428918	37.0	35.0	37.0	26.0	37.0
7	34.176306965541656	37.0	35.0	37.0	27.0	37.0
8	35.29094983099768	37.0	35.0	37.0	32.0	37.0
9	37.1247315503252	39.0	37.0	39.0	34.0	39.0
10	36.633239858329624	38.0	35.0	39.0	32.0	39.0
11	36.664512943776565	39.0	35.0	39.0	32.0	39.0
12	36.562290125339814	39.0	35.0	39.0	32.0	39.0
13	36.53191503697959	39.0	35.0	39.0	32.0	39.0
14	37.64271905677228	39.0	36.0	41.0	33.0	41.0
15	37.67182004285394	39.0	36.0	41.0	33.0	41.0
16	37.60994155055797	39.0	36.0	41.0	33.0	41.0
17	37.60560899026169	39.0	36.0	41.0	33.0	41.0
18	37.51306996169117	39.0	36.0	41.0	32.0	41.0
19	37.50158711490565	39.0	36.0	41.0	32.0	41.0
20	37.383536025854475	39.0	36.0	41.0	32.0	41.0
21	37.316569700132675	39.0	35.0	41.0	32.0	41.0
22	37.26186232099782	39.0	35.0	41.0	32.0	41.0
23	37.14764150192157	39.0	35.0	40.0	32.0	41.0
24	37.03298148508872	39.0	35.0	40.0	31.0	41.0
25	37.04219238699387	39.0	35.0	40.0	31.0	41.0
26	37.001207946967945	39.0	35.0	40.0	31.0	41.0
27	36.942291129920456	39.0	35.0	40.0	31.0	41.0
28	36.85335634068025	39.0	35.0	40.0	31.0	41.0
29	36.680238918679194	39.0	35.0	40.0	31.0	41.0
30	36.69631649680678	39.0	35.0	40.0	31.0	41.0
31	36.31799448950765	38.0	35.0	40.0	30.0	41.0
32	36.414162259375374	38.0	35.0	40.0	30.0	41.0
33	36.42494680010438	38.0	35.0	40.0	30.0	41.0
34	36.40317190212444	38.0	35.0	40.0	30.0	41.0
35	36.37022716998607	38.0	35.0	40.0	30.0	41.0
36	36.31057590648089	38.0	35.0	40.0	30.0	41.0
37	36.19775169951768	38.0	35.0	40.0	30.0	41.0
38	36.10403535143784	38.0	35.0	40.0	30.0	41.0
39	36.05168383641997	38.0	35.0	40.0	30.0	41.0
40	35.93675184775458	38.0	35.0	40.0	29.0	41.0
41	35.913238535223414	38.0	35.0	40.0	29.0	41.0
42	35.79593169341669	38.0	35.0	40.0	29.0	41.0
43	35.70264045446248	38.0	34.0	40.0	29.0	41.0
44	35.529940790996996	38.0	34.0	40.0	28.0	41.0
45	35.34445416355798	38.0	34.0	40.0	27.0	41.0
46	35.09740205645008	37.0	34.0	40.0	27.0	41.0
47	35.057557570433964	37.0	34.0	40.0	27.0	41.0
48	35.03964479498592	37.0	34.0	40.0	27.0	41.0
49	34.90197559359078	37.0	34.0	40.0	27.0	41.0
50	34.78820071031202	37.0	33.0	40.0	26.0	41.0
51	33.62977405511227	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	11.0
10	13.0
11	19.0
12	29.0
13	57.0
14	65.0
15	129.0
16	255.0
17	475.0
18	894.0
19	1596.0
20	2494.0
21	3785.0
22	5343.0
23	7117.0
24	8971.0
25	11159.0
26	13963.0
27	17058.0
28	21657.0
29	27977.0
30	36134.0
31	47165.0
32	62192.0
33	85732.0
34	123622.0
35	152555.0
36	171410.0
37	231566.0
38	307295.0
39	291739.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.124064484276474	31.5147973503573	20.707408537220324	26.6537296281459
2	23.415427173416344	30.474995130234078	20.50912637011936	25.60045132623021
3	21.374658350699104	31.38573323973582	20.025702563273267	27.213905846291812
4	21.365347603278853	31.91485321484182	19.568250841336287	27.151548340543037
5	18.732488750534447	35.48962892996235	17.20693503671007	28.570947282793124
6	18.90265491062295	43.236905842616515	17.405400968562752	20.455038278197783
7	83.48248905680904	7.638549434555859	4.835218147136762	4.043743361498344
8	84.87518085514316	6.755437292728674	4.399695685571159	3.9696861665570204
9	77.52220184475308	10.349998346117234	7.562532082262903	4.565267726866774
10	36.71834131484905	33.108772806737065	13.702296201827602	16.47058967658629
11	27.789334538830108	29.821833947720155	22.83001392936818	19.558817584081563
12	27.573472210481697	25.149431370603274	27.798155246912447	19.478941172002582
13	25.33607510342893	25.43879959963786	26.00393746608009	23.221187830853122
14	21.67431740582975	28.21266727186525	27.93420241809911	22.178812904205884
15	21.54574333454618	29.68725689454721	27.53586169129727	21.23113807960934
16	23.04863272899232	28.43851415172353	25.953953453613487	22.558899665670662
17	22.46983501600591	29.098535884968168	26.15958621078307	22.272042888242854
18	23.588962353952965	28.042501111776748	26.115788944957558	22.252747589312733
19	23.230621088107846	31.359761154826703	24.91696895968324	20.49264879738221
20	24.722790872037255	29.217982973583208	25.60939454414703	20.44983161023251
21	25.4781865114222	28.224489470892276	25.765472073270683	20.53185194441484
22	24.153487671222805	27.237060205007957	25.858273272886983	22.751178850882255
23	23.30069671342867	28.13628239006886	24.819818660943007	23.743202235559462
24	21.276956757703726	28.489478242865946	26.84974536330904	23.383819636121288
25	22.17293243215099	29.7303803562831	24.957764734564066	23.13892247700184
26	22.848022875036296	27.733592564143088	25.568598769266202	23.849785791554417
27	21.140787076682578	28.894495755646783	24.88297248061588	25.08174468705475
28	21.228320353416372	29.603888952185635	26.950019662828435	22.21777103156956
29	22.867624448552608	29.26294408283625	25.17999757430528	22.689433894305864
30	22.981007300361036	28.799856908513334	24.679544900466883	23.539590890658747
31	23.830796767210487	29.110725613498623	24.387848984577236	22.67062863471365
32	23.73389148813921	27.621128536093238	25.44241363975493	23.202566336012623
33	22.321230586785354	27.68415984593163	25.138956779755496	24.855652787527518
34	20.451424238080712	28.798999339671994	27.137214689909232	23.612361732338062
35	21.926626409935057	27.423642682916373	27.277549705302594	23.37218120184598
36	23.993367317561418	29.668451634954994	25.0612855446971	21.276895502786488
37	22.457032738303067	30.3749045954664	24.533574432687583	22.63448823354295
38	21.88717824323348	28.898599835101763	26.141332245446	23.072889676218757
39	21.816673833491983	26.479765663188616	26.796086055808132	24.907474447511273
40	20.967680680566634	26.7600681644719	27.21592725856068	25.056323896400784
41	20.50153076038179	26.541388109930526	29.410752198132705	23.54632893155498
42	21.854468117428127	27.14971069302588	26.89047988327263	24.105341306273363
43	21.935447118017397	26.11438008186107	27.93683637954037	24.01333642058116
44	22.23106334861031	26.30549542364513	25.924122308918346	25.539318918826208
45	22.56380005904974	27.65010211194704	26.255450156261297	23.53064767274193
46	21.081308552044014	26.756331614520356	28.142652901461663	24.019706931973964
47	20.991018804034496	27.29856014191539	27.871171108260718	23.839249945789398
48	20.962045228180692	26.877432585900834	28.616459686301315	23.54406249961716
49	21.377108547388644	27.351484390409443	27.756256883521324	23.515150178680592
50	20.604194001673484	26.52092896757287	28.087033436609122	24.78784359414452
51	21.61869794097721	25.2912977589276	28.32059843603945	24.769405864055738
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23041.0
1	17394.5
2	11748.0
3	7386.0
4	3024.0
5	2716.0
6	2408.0
7	2432.5
8	2457.0
9	2557.0
10	2657.0
11	3011.0
12	3365.0
13	3676.0
14	3987.0
15	4223.0
16	4459.0
17	4622.0
18	4785.0
19	5056.5
20	5328.0
21	5981.0
22	6634.0
23	7728.5
24	8823.0
25	9336.5
26	11730.0
27	13610.0
28	18354.5
29	23099.0
30	27534.0
31	31969.0
32	36557.0
33	41145.0
34	45105.0
35	49065.0
36	49841.5
37	50618.0
38	54063.0
39	57508.0
40	59567.0
41	61626.0
42	72751.0
43	83876.0
44	89104.0
45	94332.0
46	104908.0
47	115484.0
48	122357.5
49	129231.0
50	126169.0
51	123107.0
52	114370.0
53	105633.0
54	102356.0
55	99079.0
56	90001.5
57	80924.0
58	80065.5
59	79207.0
60	74076.5
61	68946.0
62	64310.5
63	59675.0
64	53013.5
65	46352.0
66	41681.0
67	37010.0
68	32376.5
69	27743.0
70	24257.0
71	20771.0
72	17224.0
73	13677.0
74	12463.5
75	9120.0
76	6990.0
77	5088.0
78	3186.0
79	3069.5
80	2953.0
81	2011.5
82	1070.0
83	735.0
84	400.0
85	290.5
86	181.0
87	121.5
88	62.0
89	87.0
90	112.0
91	78.0
92	44.0
93	29.0
94	14.0
95	8.5
96	3.0
97	2.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1632522.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.150262371449184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.26136836049203	15.917976872586905
2	9.988543179904296	4.225206179270503
3	3.904028422084448	2.477136762980425
4	2.1556741422890555	1.823722947870488
5	1.2773914086914917	1.350858172243006
6	0.894682852318436	1.1353666239462865
7	0.6319998262306907	0.9356873500442582
8	0.49845041610171803	0.8433885663767484
9	0.3889889487653952	0.7404496493384081
>10	3.0765840385940018	13.90058171606306
>50	0.83345077225955	12.888016669087953
>100	1.0765509099273982	39.931162996415814
>500	0.009361312260122353	1.2515090295698486
>1k	0.002340328065030588	1.0533910727456703
>5k	2.925410081288235E-4	0.3193894107203025
>10k+	2.925410081288235E-4	1.2061559807403288
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19494	1.1941033566469548	No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	5162	0.3161978827850406	TruSeq Adapter, Index 16 (95% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4251	0.2603946531807841	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3441	0.21077817021761422	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2402	0.14713431120683212	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1722	0.10548096748466483	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.125491723848131E-5	0.0	0.0	0.3735937402374976	0.0
2	6.125491723848131E-5	0.0	0.0	0.49567479029379086	0.0
3	6.125491723848131E-5	0.0	0.0	0.644462984266062	0.0
4	6.125491723848131E-5	0.0	0.0	1.3270877819716977	0.0
5	6.125491723848131E-5	0.0	0.0	1.430057297849585	0.0
6	6.125491723848131E-5	0.0	0.0	2.085484912301335	0.0
7	6.125491723848131E-5	0.0	0.0	2.631204969978965	0.0
8	6.125491723848131E-5	0.0	0.0	3.0378151106080042	0.0
9	6.125491723848131E-5	0.0	0.0	3.6665355811437763	0.0
10	6.125491723848131E-5	0.0	0.0	4.636078411194458	0.0
11	6.125491723848131E-5	0.0	0.0	5.479742386320062	0.0
12	6.125491723848131E-5	0.0	0.0	5.916061161809765	0.0
13	6.125491723848131E-5	0.0	0.0	6.087819949746466	0.0
14	6.125491723848131E-5	0.0	0.0	6.2897774118817384	0.0
15	6.125491723848131E-5	0.0	0.0	6.472684594755844	0.0
16	6.125491723848131E-5	0.0	0.0	6.852710101303382	0.0
17	6.125491723848131E-5	0.0	0.0	7.314755942033247	0.0
18	6.125491723848131E-5	0.0	0.0	7.941700019969103	0.0
19	6.125491723848131E-5	0.0	0.0	8.201788398563695	0.0
20	6.125491723848131E-5	0.0	0.0	8.476822976964476	0.0
21	6.125491723848131E-5	0.0	0.0	8.79210203599094	0.0
22	6.125491723848131E-5	0.0	0.0	9.149769497746432	0.0
23	6.125491723848131E-5	0.0	0.0	9.503087860377992	0.0
24	6.125491723848131E-5	0.0	0.0	9.77383459457208	0.0
25	6.125491723848131E-5	0.0	0.0	10.014688929153788	0.0
26	6.125491723848131E-5	0.0	0.0	10.217503960130399	0.0
27	6.125491723848131E-5	0.0	0.0	10.45223280298826	0.0
28	6.125491723848131E-5	0.0	0.0	10.685920312253065	0.0
29	6.125491723848131E-5	0.0	0.0	10.927264686172682	0.0
30	6.125491723848131E-5	0.0	0.0	11.234641860875382	0.0
31	6.125491723848131E-5	0.0	0.0	11.485113217463532	0.0
32	6.125491723848131E-5	0.0	0.0	11.747039243575278	0.0
33	6.125491723848131E-5	0.0	0.0	12.024769038334552	0.0
34	6.125491723848131E-5	0.0	0.0	12.282162200570651	0.0
35	1.2250983447696263E-4	0.0	0.0	12.55646171996457	0.0
36	1.8376475171544396E-4	0.0	0.0	12.808893233904351	0.0
37	1.8376475171544396E-4	0.0	0.0	13.055750550375432	0.0
38	1.8376475171544396E-4	0.0	0.0	13.312653673273623	0.0
39	1.8376475171544396E-4	0.0	0.0	13.60796362928034	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATC	20	7.034158E-4	45.000004	27
CGTATAC	20	7.034158E-4	45.000004	22
ATTCGGA	20	7.034158E-4	45.000004	27
TCGCTAT	20	7.034158E-4	45.000004	15
GCGATAT	20	7.034158E-4	45.000004	22
ACGTAAG	40	6.8175723E-9	45.000004	1
TATCGGC	40	6.8175723E-9	45.000004	40
ATCGCAT	20	7.034158E-4	45.000004	43
AATCGTC	20	7.034158E-4	45.000004	18
AGTCGAT	20	7.034158E-4	45.000004	36
ACGCATA	25	3.891529E-5	45.0	1
TGGACGT	25	3.891529E-5	45.0	17
CGCATAT	25	3.891529E-5	45.0	2
ATAGCGC	35	1.2123564E-7	45.0	21
CGCGCAT	25	3.891529E-5	45.0	12
CGTTCGA	25	3.891529E-5	45.0	19
GTTAACG	35	1.2123564E-7	45.0	1
ACTACGT	25	3.891529E-5	45.0	30
ACTCGGT	25	3.891529E-5	45.0	20
ACGTCGA	25	3.891529E-5	45.0	35
>>END_MODULE