##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553456_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	852851
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32216412949038	31.0	31.0	34.0	30.0	34.0
2	31.60718929801337	31.0	31.0	34.0	30.0	34.0
3	31.790718425610102	31.0	31.0	34.0	30.0	34.0
4	35.54804414839169	37.0	35.0	37.0	33.0	37.0
5	35.316561744079564	37.0	35.0	37.0	33.0	37.0
6	34.03865270721381	37.0	35.0	37.0	32.0	37.0
7	34.70443606210229	37.0	35.0	37.0	32.0	37.0
8	35.407332582127474	37.0	35.0	37.0	32.0	37.0
9	37.12289016487053	39.0	37.0	39.0	34.0	39.0
10	36.52359204597286	38.0	35.0	39.0	32.0	39.0
11	36.59192871908458	38.0	35.0	39.0	32.0	39.0
12	36.44220971775844	38.0	35.0	39.0	32.0	39.0
13	36.47815620782528	38.0	35.0	39.0	32.0	39.0
14	37.593179816873054	39.0	36.0	41.0	32.0	41.0
15	37.638776292693564	39.0	36.0	41.0	33.0	41.0
16	37.435692752895875	39.0	36.0	41.0	32.0	41.0
17	37.44277253588259	39.0	36.0	41.0	32.0	41.0
18	37.24672422263678	39.0	36.0	41.0	32.0	41.0
19	37.20234953116078	39.0	36.0	41.0	31.0	41.0
20	37.21727945444163	39.0	35.0	40.0	32.0	41.0
21	37.263559519775434	39.0	36.0	40.0	32.0	41.0
22	37.14143736713682	39.0	35.0	40.0	31.0	41.0
23	37.002780087025755	39.0	35.0	40.0	31.0	41.0
24	36.88749382952005	39.0	35.0	40.0	31.0	41.0
25	36.92025453449665	39.0	35.0	40.0	31.0	41.0
26	36.80700497507771	39.0	35.0	40.0	31.0	41.0
27	36.79462766649743	39.0	35.0	40.0	31.0	41.0
28	36.657474752330714	39.0	35.0	40.0	30.0	41.0
29	36.525631089135146	39.0	35.0	40.0	30.0	41.0
30	36.59891704412611	39.0	35.0	40.0	30.0	41.0
31	36.454959893345965	39.0	35.0	40.0	30.0	41.0
32	36.36540966710481	39.0	35.0	40.0	30.0	41.0
33	36.2674195140769	39.0	35.0	40.0	30.0	41.0
34	36.136099975259455	38.0	35.0	40.0	30.0	41.0
35	36.03319219887178	38.0	35.0	40.0	29.0	41.0
36	36.034954523123034	38.0	35.0	40.0	30.0	41.0
37	35.90959147611951	38.0	35.0	40.0	29.0	41.0
38	35.85788607857644	38.0	35.0	40.0	29.0	41.0
39	35.680307580104845	38.0	35.0	40.0	28.0	41.0
40	35.50000762149543	38.0	34.0	40.0	27.0	41.0
41	35.410001278066154	38.0	34.0	40.0	27.0	41.0
42	35.34491253454589	38.0	34.0	40.0	27.0	41.0
43	35.37076699212406	38.0	34.0	40.0	27.0	41.0
44	35.21523572112831	38.0	34.0	40.0	26.0	41.0
45	35.040864113426615	38.0	34.0	40.0	26.0	41.0
46	34.75768451933573	37.0	33.0	40.0	25.0	41.0
47	34.72299147213288	37.0	33.0	40.0	25.0	41.0
48	34.54934332022827	37.0	33.0	40.0	24.0	41.0
49	34.410029418972364	37.0	33.0	40.0	24.0	41.0
50	34.328644745682425	37.0	33.0	40.0	24.0	41.0
51	32.91730677457141	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	14.0
10	21.0
11	24.0
12	20.0
13	27.0
14	44.0
15	74.0
16	120.0
17	234.0
18	520.0
19	986.0
20	1876.0
21	3161.0
22	4289.0
23	5393.0
24	6239.0
25	7008.0
26	8101.0
27	9736.0
28	12107.0
29	15600.0
30	20077.0
31	26559.0
32	33982.0
33	44293.0
34	61585.0
35	76722.0
36	85597.0
37	113743.0
38	156727.0
39	157945.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.944103952507533	37.774476432577316	18.75134109006145	22.530078524853696
2	22.06059440629137	36.296961602906016	18.125205926943863	23.517238063858752
3	19.7950169490333	28.39569866248618	27.107665934612257	24.70161845386826
4	24.84478531419908	31.57925593098912	17.959760849198748	25.616197905613053
5	16.729885994153726	39.12195682481465	17.43446393332481	26.71369324770681
6	17.92200513337031	39.06426796708921	22.43123359179974	20.582493307740744
7	78.22738086723237	12.652620446009912	5.3173414816890645	3.8026572050686465
8	79.6536557968508	6.186191960846619	4.171185822611453	9.98896641969113
9	77.05191176418859	10.12791214409082	7.038861418934843	5.781314672785751
10	35.02534440365316	36.94408519190339	13.818943754536257	14.211626649907194
11	24.372369851240133	27.316377655651458	26.973879376350617	21.337373116757792
12	25.685260379597374	23.979921463420926	32.57802359380478	17.75679456317692
13	24.680864535540206	27.613615977468513	26.515065351391982	21.190454135599303
14	19.770628163653438	26.85345974853755	30.732097400366538	22.64381468744247
15	18.29147178112003	31.259856645533628	25.66626526790729	24.78240630543905
16	20.204701641904624	31.469037381676284	28.118862497669582	20.20739847874951
17	20.138218750989328	29.46528760592413	26.859908706210113	23.53658493687643
18	20.102808110678183	29.961388331607747	25.730872098408746	24.204931459305318
19	21.504928762468474	30.451860876049864	29.185637350486783	18.857573010994884
20	22.112537828999436	33.50397666180845	25.25939466565672	19.124090843535388
21	22.260629347916577	26.50310546625378	29.128417507864796	22.107847677964852
22	22.599610013941472	30.173031397043566	26.253003162334338	20.974355426680628
23	22.143961840931183	29.840265181139497	25.044116733169098	22.971656244760222
24	19.34980436207497	34.34409996587915	25.607286618647336	20.69880905339854
25	20.631036370948735	29.477833759941653	26.304360316163084	23.586769552946528
26	23.097235038711332	31.494481451038926	22.492322809025257	22.91596070122448
27	19.5707104758041	29.59438401315118	27.83991576488742	22.9949897461573
28	21.52099252976194	30.082980497179463	26.904582394814568	21.49144457824403
29	19.827613498723693	31.289404597051533	27.07776622176676	21.805215682458016
30	24.205517728184642	27.89807363771632	24.531717732640285	23.364690901458754
31	23.568477963911633	29.87532406012305	22.913498371931322	23.642699604033997
32	22.74477018846199	27.484871331569057	25.463298981885462	24.307059498083486
33	24.1518154988386	29.024530662448655	25.134753901912525	21.688899936800215
34	19.81588812113722	29.36808422573228	26.605468012583678	24.210559640546826
35	23.922115351919622	29.851873304950104	25.16266030056833	21.063351042561948
36	20.87668303138532	28.17326824967081	26.346454421698517	24.60359429724536
37	22.81875732103263	30.115577046869852	24.76645979192145	22.299205840176068
38	24.074310752992023	28.915015635791015	25.48030077938585	21.53037283183112
39	20.676296328432517	28.5827184349904	28.107488881410703	22.633496355166375
40	23.53013597920387	29.957753464555942	24.778302423283787	21.7338081329564
41	20.116526802454356	27.700852786711867	30.048038871971777	22.134581538862005
42	21.92563531027108	30.08169070564495	24.787213710249503	23.20546027383447
43	22.362874640470608	26.583541556496975	26.681448459344008	24.372135343688406
44	21.655599864454636	26.122734217348636	27.350381250652223	24.871284667544508
45	24.85393110871653	28.388663435934298	25.772028173737265	20.98537728161191
46	19.040957916447304	27.05466722792141	31.7085868457679	22.19578800986339
47	22.994520731053843	28.768213908408384	25.00530573335788	23.231959627179894
48	20.508271667618377	27.038486206852074	29.840734196242952	22.612507929286593
49	21.8923352379255	26.632670888584293	27.771439559782422	23.703554313707787
50	21.878616546149328	26.444478578321416	26.21219884833341	25.464706027195838
51	20.40590912128848	26.01919913326009	29.7478692057581	23.827022539693335
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11936.0
1	9257.5
2	6579.0
3	4194.0
4	1809.0
5	1543.0
6	1277.0
7	1341.5
8	1406.0
9	1559.5
10	1713.0
11	1787.5
12	1862.0
13	2068.0
14	2274.0
15	2286.5
16	2299.0
17	2467.5
18	2636.0
19	2820.5
20	3005.0
21	3644.0
22	4283.0
23	5003.0
24	5723.0
25	7034.5
26	9321.5
27	10297.0
28	12181.5
29	14066.0
30	14203.0
31	14340.0
32	17170.0
33	20000.0
34	24734.5
35	29469.0
36	29672.5
37	29876.0
38	30832.5
39	31789.0
40	36771.5
41	41754.0
42	41259.0
43	40764.0
44	59123.5
45	77483.0
46	76848.0
47	76213.0
48	70947.0
49	65681.0
50	62164.0
51	58647.0
52	54311.5
53	49976.0
54	46217.5
55	42459.0
56	39943.5
57	37428.0
58	34908.5
59	32389.0
60	30920.5
61	29452.0
62	26914.5
63	24377.0
64	22248.5
65	20120.0
66	17825.0
67	15530.0
68	13551.0
69	11572.0
70	9871.5
71	8171.0
72	6571.5
73	4972.0
74	4657.0
75	3576.0
76	2810.0
77	2409.5
78	2009.0
79	1313.0
80	617.0
81	593.5
82	570.0
83	386.5
84	203.0
85	212.0
86	221.0
87	132.5
88	44.0
89	40.0
90	36.0
91	24.0
92	12.0
93	11.0
94	10.0
95	7.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	852851.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.700452213391983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.26631703520442	16.984144727406402
2	8.87092169714519	3.850060247552826
3	3.102655790360697	2.0198710113997866
4	1.5768394942395476	1.3687252037173794
5	0.9893429378854542	1.0734594573120066
6	0.6749920082698018	0.878857909192819
7	0.5014663573223814	0.7617432706588659
8	0.38612826567716535	0.67033263820538
9	0.2827734976155933	0.5522681494998805
>10	3.280998500249721	16.687189202283566
>50	1.233118895152359	19.42009317230635
>100	0.8239249735009921	27.421189342714484
>500	0.0033222781189556137	0.45636095475476257
>1k	0.00498341717843342	2.286010311798146
>5k	5.537130198259355E-4	1.1662557732621712
>10k+	0.0016611390594778069	4.403438627935173
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGT	13521	1.5853883034668423	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCC	12827	1.5040141830167286	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10299	1.2075966376307232	No Hit
AATCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCT	9706	1.1380651485429458	No Hit
CTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTGCT	3010	0.352933865352799	No Hit
AATGACTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTT	2878	0.3374563669386563	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2297	0.2693319231612556	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2221	0.2604206361955371	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCT	2175	0.25502696250576007	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACGCTGAATCGTAT	2003	0.23485931305702873	No Hit
AACTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTG	1997	0.23415579040184042	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGCTGAATC	1326	0.1554785067966151	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1118	0.13108972141675393	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	930	0.10904601155418707	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4489647077860025	0.0
2	0.0	0.0	0.0	0.5595350184264308	0.0
3	0.0	0.0	0.0	0.9968916024018264	0.0
4	0.0	0.0	0.0	2.8261677596672805	0.0
5	0.0	0.0	0.0	2.9992343328436033	0.0
6	0.0	0.0	0.0	3.784951884913074	0.0
7	0.0	0.0	0.0	4.343079858029128	0.0
8	0.0	0.0	0.0	4.577470155982698	0.0
9	0.0	0.0	0.0	6.781606634687654	0.0
10	0.0	0.0	0.0	7.376904054752823	0.0
11	0.0	0.0	0.0	9.678830182528953	0.0
12	0.0	0.0	0.0	10.074092660968915	0.0
13	0.0	0.0	0.0	10.385987704769063	0.0
14	0.0	0.0	0.0	10.81795061505468	0.0
15	0.0	0.0	0.0	11.002508058265747	0.0
16	0.0	0.0	0.0	11.301153425393181	0.0
17	0.0	0.0	0.0	11.601440345382722	0.0
18	0.0	0.0	0.0	12.255012892052656	0.0
19	0.0	0.0	0.0	12.453289027039894	0.0
20	0.0	0.0	0.0	12.672905349234508	0.0
21	0.0	0.0	0.0	12.897680837567172	0.0
22	0.0	0.0	0.0	13.13078134398623	0.0
23	0.0	0.0	0.0	13.374551944008976	0.0
24	0.0	0.0	0.0	13.54140406706447	0.0
25	0.0	0.0	0.0	13.693247706809279	0.0
26	0.0	0.0	0.0	13.84016668796777	0.0
27	0.0	0.0	0.0	13.99705224007476	0.0
28	0.0	0.0	0.0	14.157220897905965	0.0
29	0.0	0.0	0.0	14.326535350254616	0.0
30	0.0	0.0	0.0	14.531612204242007	0.0
31	0.0	0.0	0.0	14.690139309211105	0.0
32	0.0	0.0	0.0	14.859571015335622	0.0
33	0.0	0.0	0.0	15.031465050753297	0.0
34	0.0	0.0	0.0	15.20007598044676	0.0
35	0.0	0.0	0.0	15.3681006412609	0.0
36	0.0	0.0	0.0	15.538704885144064	0.0
37	0.0	0.0	0.0	15.708957367699634	0.0
38	0.0	0.0	0.0	15.887652122117462	0.0
39	0.0	0.0	0.0	16.074320133294094	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTG	35	1.2114288E-7	45.0	41
CTCGTAC	35	1.2114288E-7	45.0	29
CGAGTTA	20	7.032262E-4	45.0	28
GATCGGT	35	1.2114288E-7	45.0	27
ACGTTAC	20	7.032262E-4	45.0	38
TAGACCG	20	7.032262E-4	45.0	1
TTAGATC	20	7.032262E-4	45.0	17
TAGAACG	20	7.032262E-4	45.0	1
TAGCGAG	20	7.032262E-4	45.0	1
ATAGCGG	45	3.8380676E-10	45.0	2
TTCGTGG	20	7.032262E-4	45.0	41
ATATGCG	20	7.032262E-4	45.0	1
GCGTTAG	25	3.889956E-5	45.0	1
GTAATCG	20	7.032262E-4	45.0	9
CGTTACT	20	7.032262E-4	45.0	39
GCCGATT	20	7.032262E-4	45.0	39
ACGATAG	25	3.889956E-5	45.0	1
ACCTACG	25	3.889956E-5	45.0	1
TCTCGAC	35	1.2114288E-7	45.0	43
TCGTCGT	25	3.889956E-5	45.0	14
>>END_MODULE