FastQCFastQC Report
Sat 18 Jun 2016
SRR3553455_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553455_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843213
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGT119761.4202817081804953No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCC115081.3647797175802554No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA103301.2250759891035836No Hit
AATCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCT90941.0784938088003861No Hit
CTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTGCT29240.3467688472544897No Hit
AATGACTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTT26260.31142783614579No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21950.26031382343488535No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21060.2497589577010791No Hit
AACTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTG19660.23315579811981077No Hit
AATGATCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCT19210.22781906825440312No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACGCTGAATCGTAT18310.21714560852358775No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGCTGAATC13940.16532003183062882No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10560.12523526084156672No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG9140.10839491326628029No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTT207.0322165E-445.031
CGAACTT207.0322165E-445.033
TACCCGA253.889919E-545.013
AAATCGT253.889919E-545.039
CATATTA207.0322165E-445.027
CGTATAG207.0322165E-445.01
GATCGGT351.2114106E-745.027
CGACGTA207.0322165E-445.013
CGAAAGT207.0322165E-445.043
ACACCGT502.1827873E-1145.043
CGTTGTA207.0322165E-445.021
CGCGCAT207.0322165E-445.019
TAACGTA253.889919E-545.026
ATATGCG406.8121153E-945.01
ACGATAG253.889919E-545.01
ACCTACG207.0322165E-445.01
GTTAACG253.889919E-545.01
CGTAGGT253.889919E-545.020
CGTAGAC253.889919E-545.01
TACGACA253.889919E-545.015