##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553455_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	843213
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30671253882471	31.0	31.0	34.0	30.0	34.0
2	31.602663858360817	31.0	31.0	34.0	30.0	34.0
3	31.786057615335626	31.0	31.0	34.0	30.0	34.0
4	35.55322083506777	37.0	35.0	37.0	33.0	37.0
5	35.33528657646407	37.0	35.0	37.0	33.0	37.0
6	32.712879189481185	37.0	35.0	37.0	26.0	37.0
7	34.05136424604459	37.0	35.0	37.0	26.0	37.0
8	35.11350750047734	37.0	35.0	37.0	32.0	37.0
9	36.99713832685217	39.0	37.0	39.0	33.0	39.0
10	36.48190670684631	38.0	35.0	39.0	32.0	39.0
11	36.58962919214955	38.0	35.0	39.0	32.0	39.0
12	36.44774689194782	38.0	35.0	39.0	32.0	39.0
13	36.5033544312054	39.0	35.0	39.0	32.0	39.0
14	37.59642462817817	39.0	36.0	41.0	32.0	41.0
15	37.68567728438722	39.0	36.0	41.0	33.0	41.0
16	37.50371258507637	39.0	36.0	41.0	32.0	41.0
17	37.53350221118507	39.0	36.0	41.0	32.0	41.0
18	37.346440341882776	39.0	36.0	41.0	32.0	41.0
19	37.27725497590763	39.0	36.0	41.0	32.0	41.0
20	37.27546183467285	39.0	36.0	40.0	32.0	41.0
21	37.31403334626008	39.0	36.0	40.0	32.0	41.0
22	37.153095362618934	39.0	35.0	40.0	31.0	41.0
23	37.012358680428314	39.0	35.0	40.0	31.0	41.0
24	36.97148881717905	39.0	35.0	40.0	31.0	41.0
25	36.965350391893864	39.0	35.0	40.0	31.0	41.0
26	36.85009837372052	39.0	35.0	40.0	31.0	41.0
27	36.81192652390321	39.0	35.0	40.0	31.0	41.0
28	36.70278565439575	39.0	35.0	40.0	30.0	41.0
29	36.55024412574284	39.0	35.0	40.0	30.0	41.0
30	36.631056447184754	39.0	35.0	40.0	30.0	41.0
31	36.16220219564926	39.0	35.0	40.0	29.0	41.0
32	36.251660019473135	38.0	35.0	40.0	30.0	41.0
33	36.20215769918158	38.0	35.0	40.0	30.0	41.0
34	36.11564575024342	38.0	35.0	40.0	30.0	41.0
35	36.100335265229546	38.0	35.0	40.0	30.0	41.0
36	36.072335222535706	38.0	35.0	40.0	30.0	41.0
37	35.92520395202636	38.0	35.0	40.0	29.0	41.0
38	35.86297056615588	38.0	35.0	40.0	29.0	41.0
39	35.687840438892664	38.0	35.0	40.0	28.0	41.0
40	35.52323671480397	38.0	34.0	40.0	27.0	41.0
41	35.4985845806457	38.0	34.0	40.0	27.0	41.0
42	35.42093041734414	38.0	34.0	40.0	27.0	41.0
43	35.46726983573546	38.0	34.0	40.0	27.0	41.0
44	35.25931763386001	38.0	34.0	40.0	26.0	41.0
45	35.09318760502981	38.0	34.0	40.0	26.0	41.0
46	34.712458180791806	37.0	33.0	40.0	24.0	41.0
47	34.73878960594772	37.0	33.0	40.0	25.0	41.0
48	34.68523967253825	37.0	33.0	40.0	24.0	41.0
49	34.60270655219974	37.0	33.0	40.0	25.0	41.0
50	34.487146189634174	37.0	33.0	40.0	24.0	41.0
51	33.162380086644774	36.0	31.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	12.0
10	15.0
11	23.0
12	28.0
13	27.0
14	40.0
15	77.0
16	136.0
17	266.0
18	498.0
19	1068.0
20	2100.0
21	3284.0
22	4424.0
23	5153.0
24	6247.0
25	6922.0
26	7800.0
27	9451.0
28	11833.0
29	15078.0
30	19531.0
31	25761.0
32	33895.0
33	44241.0
34	61292.0
35	76544.0
36	86663.0
37	115483.0
38	152508.0
39	152788.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.95745677545294	37.70115024317699	18.736309805470267	22.6050831758998
2	22.210046571862627	36.086374379901635	18.097206755588445	23.60637229264729
3	19.75953881166443	28.50098373720519	27.059592297557085	24.6798851535733
4	24.815200904160633	31.64052262002602	17.921687640015037	25.62258883579831
5	16.816747369881632	39.032130671609664	17.43580803426892	26.71531392423978
6	17.195418002331557	41.47765748393348	21.473696444433376	19.853228069301586
7	78.37094541948476	12.638325073261441	5.240550133833326	3.7501793734204765
8	79.60610189833412	6.371699677305734	4.087579294911251	9.934619129448906
9	77.38863134225872	10.089977265530774	6.920789883457679	5.600601508752829
10	35.1905153264952	36.99243251705085	13.670092847240259	14.146959309213686
11	24.40593302048237	27.35074056021432	26.793941744256788	21.449384675046517
12	25.881005155281052	24.05797823325779	32.32789342669053	17.733123184770633
13	24.601850303541337	27.788115221183734	26.557228126226708	21.052806349048222
14	19.88038609461666	26.859880006593826	30.47687832137313	22.782855577416385
15	18.37507249058067	31.111237611374587	25.715803717447432	24.797886180597313
16	20.23889574757505	31.65843031357439	28.063846264229795	20.038827674620766
17	20.159674957573	29.396842790611625	26.80556395596368	23.6379182958517
18	20.210314594295866	29.935259537032756	25.64512169523003	24.20930417344135
19	21.62442941463189	30.366230122163678	29.19309830375006	18.816242159454372
20	22.257839952657278	33.54727690393768	25.250203685189867	18.944679458215184
21	22.304803175472866	26.519278047183807	29.254174212209726	21.9217445651336
22	22.868243255262904	29.965975382258105	26.22635087457143	20.93943048790756
23	22.077695671200516	30.016496424983963	25.160546623451012	22.74526128036451
24	19.320266646742876	34.293588927115685	25.732169689034677	20.653974737106758
25	20.662987880879445	29.491006424236815	26.436380843274478	23.409624851609262
26	23.101161865388697	31.45610895467693	22.63081807325077	22.811911106683603
27	19.640707626661353	29.729498952222038	27.718856326930442	22.910937094186167
28	21.34205710775332	30.196166330452684	27.118533514070585	21.34324304772341
29	19.933397611279712	31.358031719150443	27.132290417723638	21.57628025184621
30	23.95954521574027	28.013918191488983	24.671939355773688	23.35459723699706
31	23.675038216915535	29.82733899975451	22.89872191249423	23.59890087083572
32	22.86006026946928	27.42308289838985	25.652474523044592	24.064382309096278
33	23.99607216681906	29.01117511233816	25.213202358123038	21.77955036271974
34	19.94751029692379	29.28180661351284	26.75943089112715	24.01125219843622
35	23.802052387712237	29.903950721822365	25.189720746715246	21.104276143750155
36	20.868392683699135	28.284549692663656	26.45084931090958	24.396208312727627
37	22.758780996023543	30.145408099732805	24.787331314863504	22.308479589380145
38	23.767185752591573	29.06999773485466	25.49759076295076	21.665225749603007
39	20.704021403844582	28.575342173329872	28.047480292642547	22.673156130183003
40	23.40476249773189	29.90122305989115	24.89371013018063	21.800304312196324
41	20.217786016107436	27.716247258996248	29.873353470594026	22.192613254302294
42	21.93063911490928	30.14813576166401	24.90628109386359	23.01494402956311
43	22.240406635096946	26.71697424019791	26.735237715737302	24.30738140896784
44	21.812400899891248	26.201920511187566	27.25681411458315	24.72886447433804
45	24.747009355880422	28.307556928083415	25.867841221613048	21.07759249442312
46	19.11142261800992	27.253374888669885	31.552170092254272	22.08303240106592
47	22.90536317632674	28.77588462227219	25.068873463763016	23.249878737638056
48	20.549256237747755	27.208664951797466	29.720485808449347	22.521593002005424
49	21.903362495597197	26.758126357160055	27.85772989742805	23.480781249814697
50	21.81999091569983	26.520701175147916	26.34150564566723	25.317802263485028
51	20.549137643750747	26.12530878911971	29.652650042160168	23.672903524969374
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11903.0
1	9175.5
2	6448.0
3	4117.0
4	1786.0
5	1511.5
6	1237.0
7	1327.5
8	1418.0
9	1542.5
10	1667.0
11	1709.0
12	1751.0
13	2013.0
14	2275.0
15	2256.0
16	2237.0
17	2386.5
18	2536.0
19	2838.0
20	3140.0
21	3762.0
22	4384.0
23	5127.5
24	5871.0
25	7078.5
26	9269.5
27	10253.0
28	12205.5
29	14158.0
30	14289.0
31	14420.0
32	17263.0
33	20106.0
34	24548.0
35	28990.0
36	29514.5
37	30039.0
38	30878.0
39	31717.0
40	36795.0
41	41873.0
42	41269.5
43	40666.0
44	58072.5
45	75479.0
46	74869.5
47	74260.0
48	69415.5
49	64571.0
50	61350.5
51	58130.0
52	53760.0
53	49390.0
54	45732.5
55	42075.0
56	39726.0
57	37377.0
58	34869.0
59	32361.0
60	30646.0
61	28931.0
62	26387.0
63	23843.0
64	21705.0
65	19567.0
66	17460.0
67	15353.0
68	13375.5
69	11398.0
70	9643.5
71	7889.0
72	6389.5
73	4890.0
74	4590.0
75	3531.5
76	2773.0
77	2334.5
78	1896.0
79	1220.5
80	545.0
81	566.5
82	588.0
83	395.5
84	203.0
85	187.5
86	172.0
87	105.0
88	38.0
89	29.0
90	20.0
91	11.5
92	3.0
93	5.5
94	8.0
95	5.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	843213.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.000519372942353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.13361282957145	16.969795433620213
2	8.865450837662097	3.9008904580770594
3	3.3504322565034093	2.2113374930080267
4	1.6987105594134078	1.4949005828558566
5	1.1071115891247771	1.2178514982274327
6	0.7862505355641489	1.0378752083799214
7	0.6107220457035849	0.9405341538589294
8	0.49853788705653734	0.8774473953866477
9	0.3618887699331399	0.716556680438789
>10	3.593625544823784	17.847665197735104
>50	1.223954069812872	19.408891536766063
>100	0.7587308285751677	25.439062239776923
>500	0.0038402861894621648	0.5834519961240691
>1k	0.004937510815022783	2.1748493004053784
>5k	5.486123127803092E-4	1.0976235938668355
>10k+	0.0016458369383409277	4.081267231472749
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGT	11976	1.4202817081804953	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCC	11508	1.3647797175802554	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10330	1.2250759891035836	No Hit
AATCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCT	9094	1.0784938088003861	No Hit
CTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTGCT	2924	0.3467688472544897	No Hit
AATGACTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTT	2626	0.31142783614579	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2195	0.26031382343488535	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2106	0.2497589577010791	No Hit
AACTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTG	1966	0.23315579811981077	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCT	1921	0.22781906825440312	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACGCTGAATCGTAT	1831	0.21714560852358775	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGCTGAATC	1394	0.16532003183062882	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1056	0.12523526084156672	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	914	0.10839491326628029	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.42124587737617897	0.0
2	0.0	0.0	0.0	0.5219321808368704	0.0
3	0.0	0.0	0.0	0.9257447406527176	0.0
4	0.0	0.0	0.0	2.6297032896788832	0.0
5	0.0	0.0	0.0	2.79526050950353	0.0
6	0.0	0.0	0.0	3.5312548549417526	0.0
7	0.0	0.0	0.0	4.069078631377837	0.0
8	0.0	0.0	0.0	4.286105645904415	0.0
9	1.185939970090594E-4	0.0	0.0	6.356282457694556	0.0
10	1.185939970090594E-4	0.0	0.0	6.947354938787709	0.0
11	1.185939970090594E-4	0.0	0.0	9.14371576339549	0.0
12	1.185939970090594E-4	0.0	0.0	9.532822667582213	0.0
13	1.185939970090594E-4	0.0	0.0	9.844250503728002	0.0
14	1.185939970090594E-4	0.0	0.0	10.262294343184937	0.0
15	1.185939970090594E-4	0.0	0.0	10.445166286572906	0.0
16	1.185939970090594E-4	0.0	0.0	10.728843127418576	0.0
17	1.185939970090594E-4	0.0	0.0	11.02722562389337	0.0
18	1.185939970090594E-4	0.0	0.0	11.714003460572833	0.0
19	1.185939970090594E-4	0.0	0.0	11.911343871595907	0.0
20	1.185939970090594E-4	0.0	0.0	12.133944803981912	0.0
21	1.185939970090594E-4	0.0	0.0	12.357968864332026	0.0
22	1.185939970090594E-4	0.0	0.0	12.585432150595402	0.0
23	1.185939970090594E-4	0.0	0.0	12.819418106694275	0.0
24	1.185939970090594E-4	0.0	0.0	12.979757190650524	0.0
25	1.185939970090594E-4	0.0	0.0	13.124204679007558	0.0
26	1.185939970090594E-4	0.0	0.0	13.271379829295801	0.0
27	1.185939970090594E-4	0.0	0.0	13.426382183386641	0.0
28	1.185939970090594E-4	0.0	0.0	13.576047807612074	0.0
29	1.185939970090594E-4	0.0	0.0	13.739944711478595	0.0
30	1.185939970090594E-4	0.0	0.0	13.93586199453756	0.0
31	1.185939970090594E-4	0.0	0.0	14.100944838374172	0.0
32	1.185939970090594E-4	0.0	0.0	14.262351268303501	0.0
33	1.185939970090594E-4	0.0	0.0	14.434075375972618	0.0
34	1.185939970090594E-4	0.0	0.0	14.604969325662674	0.0
35	1.185939970090594E-4	0.0	0.0	14.768984823526203	0.0
36	1.185939970090594E-4	0.0	0.0	14.934660637347859	0.0
37	1.185939970090594E-4	0.0	0.0	15.100099263175496	0.0
38	2.371879940181188E-4	0.0	0.0	15.26850273892836	0.0
39	2.371879940181188E-4	0.0	0.0	15.457304382166784	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGTT	20	7.0322165E-4	45.0	31
CGAACTT	20	7.0322165E-4	45.0	33
TACCCGA	25	3.889919E-5	45.0	13
AAATCGT	25	3.889919E-5	45.0	39
CATATTA	20	7.0322165E-4	45.0	27
CGTATAG	20	7.0322165E-4	45.0	1
GATCGGT	35	1.2114106E-7	45.0	27
CGACGTA	20	7.0322165E-4	45.0	13
CGAAAGT	20	7.0322165E-4	45.0	43
ACACCGT	50	2.1827873E-11	45.0	43
CGTTGTA	20	7.0322165E-4	45.0	21
CGCGCAT	20	7.0322165E-4	45.0	19
TAACGTA	25	3.889919E-5	45.0	26
ATATGCG	40	6.8121153E-9	45.0	1
ACGATAG	25	3.889919E-5	45.0	1
ACCTACG	20	7.0322165E-4	45.0	1
GTTAACG	25	3.889919E-5	45.0	1
CGTAGGT	25	3.889919E-5	45.0	20
CGTAGAC	25	3.889919E-5	45.0	1
TACGACA	25	3.889919E-5	45.0	15
>>END_MODULE