##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553453_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	308517
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.451543999196154	31.0	31.0	34.0	30.0	34.0
2	31.66368141787973	31.0	31.0	34.0	30.0	34.0
3	31.854183075811058	33.0	31.0	34.0	30.0	34.0
4	35.623401627787125	37.0	35.0	37.0	33.0	37.0
5	35.373318163990966	37.0	35.0	37.0	33.0	37.0
6	32.555528544618284	37.0	35.0	37.0	26.0	37.0
7	34.15216017269713	37.0	35.0	37.0	27.0	37.0
8	35.26014125639754	37.0	35.0	37.0	32.0	37.0
9	37.051361837435216	39.0	37.0	39.0	34.0	39.0
10	36.52185130803165	38.0	35.0	39.0	32.0	39.0
11	36.651785801106584	39.0	35.0	39.0	32.0	39.0
12	36.70986687929676	39.0	35.0	39.0	32.0	39.0
13	36.72243668906414	39.0	35.0	39.0	32.0	39.0
14	37.80908669538469	39.0	37.0	41.0	33.0	41.0
15	37.864594171471914	39.0	37.0	41.0	33.0	41.0
16	37.788789596683486	39.0	37.0	41.0	33.0	41.0
17	37.77560393754639	39.0	37.0	41.0	33.0	41.0
18	37.5814655270212	39.0	37.0	40.0	33.0	41.0
19	37.52104746253853	39.0	36.0	41.0	32.0	41.0
20	37.40245756311646	39.0	36.0	40.0	32.0	41.0
21	37.35156895730219	39.0	36.0	40.0	32.0	41.0
22	37.26711656083781	39.0	35.0	40.0	32.0	41.0
23	37.10195548381451	39.0	35.0	40.0	32.0	41.0
24	36.95144189785328	39.0	35.0	40.0	31.0	41.0
25	36.930934113841374	39.0	35.0	40.0	31.0	41.0
26	36.869349176868695	39.0	35.0	40.0	31.0	41.0
27	36.777425555155794	39.0	35.0	40.0	31.0	41.0
28	36.6865748078712	39.0	35.0	40.0	31.0	41.0
29	36.501307869582554	38.0	35.0	40.0	30.0	41.0
30	36.48580143071533	38.0	35.0	40.0	30.0	41.0
31	36.04980276613606	38.0	35.0	40.0	30.0	41.0
32	36.10889513381758	38.0	35.0	40.0	30.0	41.0
33	36.05021765413251	38.0	35.0	40.0	30.0	41.0
34	36.03968338859771	38.0	35.0	40.0	30.0	41.0
35	36.04274967019646	38.0	35.0	40.0	30.0	41.0
36	35.99769218551976	38.0	35.0	40.0	30.0	41.0
37	35.906611305049644	38.0	35.0	40.0	30.0	41.0
38	35.802711033751784	38.0	35.0	40.0	29.0	41.0
39	35.73450409539831	38.0	35.0	40.0	29.0	41.0
40	35.59802863375438	38.0	35.0	40.0	28.0	41.0
41	35.556517144922324	38.0	35.0	40.0	28.0	41.0
42	35.40272983336413	38.0	34.0	40.0	27.0	41.0
43	35.389479347977584	38.0	34.0	40.0	27.0	41.0
44	35.19303312297215	38.0	34.0	40.0	26.0	41.0
45	35.00352330665733	38.0	34.0	40.0	26.0	41.0
46	34.7161226123682	37.0	33.0	40.0	24.0	41.0
47	34.71491036150358	37.0	33.0	40.0	25.0	41.0
48	34.66475105099557	37.0	33.0	40.0	24.0	41.0
49	34.54479655902268	37.0	33.0	40.0	24.0	41.0
50	34.45334292761825	37.0	33.0	40.0	24.0	41.0
51	33.33394270007812	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	3.0
12	1.0
13	2.0
14	10.0
15	12.0
16	42.0
17	81.0
18	171.0
19	339.0
20	592.0
21	873.0
22	1300.0
23	1755.0
24	2175.0
25	2521.0
26	2857.0
27	3475.0
28	4247.0
29	5360.0
30	7015.0
31	8963.0
32	11634.0
33	15595.0
34	21841.0
35	32274.0
36	35224.0
37	43700.0
38	55586.0
39	50859.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.024180191042955	36.34613327628624	20.239727470447335	23.389959062223475
2	20.652022416917056	35.50015072102996	19.54446594515051	24.303360916902474
3	18.985663674935253	35.9889406418447	20.547328024063503	24.478067659156547
4	19.509783901697475	36.58534213673801	18.821329132592368	25.083544828972148
5	16.24092027343712	40.01432660112732	17.29531922065883	26.44943390477672
6	16.840887212049903	47.09432543425484	16.992904766998254	19.071882586697004
7	81.12356855537945	11.245085359963957	4.168651970555917	3.4626941141006817
8	82.27812405799357	10.531024222328105	3.302249146724492	3.8886025729538405
9	74.63737816716744	15.333352781208168	6.657655817993822	3.371613233630562
10	27.402379771617124	49.51072388231443	11.438915845804283	11.647980500264167
11	17.746834048042732	33.03124301091998	32.044263363121	17.17765957791629
12	21.5686655840683	29.725428420476018	31.89192167692542	16.81398431853026
13	22.365056058499206	31.263755319804094	28.29665788270987	18.07453073898683
14	17.91278924662174	34.93421756337576	28.755951860027164	18.397041329975334
15	16.807177562338545	36.400263194572744	28.515122343339268	18.277436899749446
16	18.255720106185397	35.808723668387806	27.292175147560748	18.64338107786605
17	17.58606494941932	35.76723486874305	27.841901742853715	18.804798438983912
18	17.397420563534585	35.399021771895875	27.62376141347154	19.579796251097996
19	17.571803174541436	37.406042454710764	27.19558403588781	17.826570334859994
20	18.84466658239254	37.06408398888878	26.21605940677499	17.875190021943684
21	19.07350324293313	37.303941111835	25.88739032208922	17.735165323142645
22	17.440854150662684	36.14355124677084	26.221245506730583	20.194349095835886
23	17.726737910714807	36.65567861738575	25.09488942262501	20.52269404927443
24	16.100571443388855	39.219880914179775	25.3211330331878	19.358414609243574
25	17.31379470175063	39.56475656122677	24.234969223738076	18.886479513284517
26	18.905279125623547	38.89801858568572	23.405517362090258	18.79118492660048
27	17.103109391054623	36.93086604627946	25.653367561593036	20.312657001072875
28	17.91894774031901	37.53180537863391	25.97360923385097	18.575637647196103
29	17.92316144653293	37.205081081431494	24.04405591912277	20.827701552912806
30	19.353552640535206	37.371360411257726	24.035952637942156	19.239134310264912
31	19.18305960449505	37.87181905697255	22.372835208432598	20.5722861300998
32	19.94606456046182	36.6365548737995	22.701180161871147	20.716200403867536
33	19.295533147281997	36.83751624707877	24.13092309337897	19.736027512260264
34	16.934237011250598	37.462117160480624	24.40967596599215	21.19396986227663
35	18.75812353938357	36.33446455138615	24.317622691780354	20.58978921744993
36	20.403089619048544	36.012278091644866	25.0381664543607	18.546465834945884
37	19.615450688292704	37.10848996975856	23.444737243004436	19.831322098944305
38	19.49325320808902	36.076131947348124	25.525983981433765	18.9046308631291
39	17.45511592554057	34.836005795466704	26.62381651578357	21.085061763209158
40	19.54025223893659	35.22269437340568	26.72235241493986	18.514700972717872
41	17.521886962468844	35.52640535205515	27.595237863715777	19.35646982176023
42	18.43366816091172	37.56324610961471	25.118875134919634	18.884210594553945
43	18.157184207029108	35.33840922866487	25.542838806289442	20.961567758016574
44	21.03255250115877	33.70284295516941	23.847308252057424	21.417296291614402
45	19.96032633533971	35.22917699835017	25.47963321308064	19.330863453229483
46	17.439881756921014	35.06354593101839	27.64807125701339	19.848501055047212
47	18.606105984435217	35.47357195875754	25.723055779746336	20.19726627706091
48	18.731544777111147	33.93167961570999	27.359918578230698	19.97685702894816
49	18.242106593801964	34.690146734215624	25.873128547211337	21.194618124771083
50	19.014835487185472	31.98235429490109	26.22448681920283	22.778323398710608
51	16.89598952407809	33.11648952894006	28.189046308631294	21.79847463835056
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11093.0
1	8323.0
2	5553.0
3	3488.0
4	1423.0
5	1338.0
6	1253.0
7	1311.0
8	1369.0
9	1846.5
10	2324.0
11	2522.0
12	2720.0
13	2947.5
14	3175.0
15	3121.0
16	3067.0
17	3062.0
18	3057.0
19	2986.0
20	2915.0
21	2861.5
22	2808.0
23	3013.5
24	3219.0
25	3154.0
26	3960.5
27	4832.0
28	5308.5
29	5785.0
30	6808.0
31	7831.0
32	8286.5
33	8742.0
34	10268.0
35	11794.0
36	11508.5
37	11223.0
38	11914.0
39	12605.0
40	13533.5
41	14462.0
42	15872.0
43	17282.0
44	18927.0
45	20572.0
46	22898.0
47	25224.0
48	26830.0
49	28436.0
50	26387.0
51	24338.0
52	21130.0
53	17922.0
54	15631.5
55	13341.0
56	11475.0
57	9609.0
58	8513.5
59	7418.0
60	6927.5
61	6437.0
62	5447.0
63	4457.0
64	3700.0
65	2943.0
66	2474.5
67	2006.0
68	1683.0
69	1360.0
70	1270.5
71	1181.0
72	864.0
73	547.0
74	529.5
75	407.0
76	302.0
77	213.0
78	124.0
79	96.0
80	68.0
81	62.0
82	56.0
83	37.5
84	19.0
85	15.5
86	12.0
87	10.0
88	8.0
89	5.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	308517.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.95781110185253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.89440885779595	25.092923774760933
2	10.134737584990441	6.883070089559011
3	4.257275521619128	4.337032739150492
4	2.3536963762312824	3.197055077407069
5	1.5425995244918058	2.6191651629250137
6	1.1124871383273733	2.2666576857936858
7	0.9069442750496934	2.1558489660430857
8	0.6315677378363878	1.7157326351577902
9	0.5477577109912484	1.6740587591481553
>10	4.041833199541426	28.001605029572023
>50	0.4519749292833243	10.567135637857723
>100	0.11972845808829782	6.14736314127707
>500	0.0	0.0
>1k	0.003990948602943261	2.0636898640607715
>5k	9.977371507358152E-4	3.2786614372871585
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9677	3.136618079392708	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2117	0.6861858503745336	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1812	0.5873258199710227	No Hit
CTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCT	1138	0.3688613593416246	TruSeq Adapter, Index 22 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1024	0.33191039715801723	No Hit
GATGGAGGGACAAAGAGGTGCAGACAGCACTGGTGGTGGGGTGGAAGGAGC	472	0.15298994869002355	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCC	458	0.1484521112288788	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGT	414	0.13419033635099523	No Hit
AATCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCT	381	0.12349400519258258	No Hit
AGTAATGGGAAAGGCATACCTGCCAGACCACATGGAGATGCCCACTGTGAT	339	0.10988049280914829	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	322	0.10437026160632965	No Hit
AGAAACGGATGGAAGGACCATGAGCATACTGTGCCCAGTGAAACACTTGTC	319	0.10339786786465577	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.241312472246262E-4	0.0	0.0	0.49267949578143183	0.0
2	3.241312472246262E-4	0.0	0.0	0.6835928003967366	0.0
3	3.241312472246262E-4	0.0	0.0	0.9101605422067504	0.0
4	3.241312472246262E-4	0.0	0.0	2.0167446202316244	0.0
5	3.241312472246262E-4	0.0	0.0	2.202471824891335	0.0
6	3.241312472246262E-4	0.0	0.0	3.2565466408658192	0.0
7	3.241312472246262E-4	0.0	0.0	4.031220321732676	0.0
8	3.241312472246262E-4	0.0	0.0	4.825666008680235	0.0
9	3.241312472246262E-4	0.0	0.0	6.0187931297140835	0.0
10	3.241312472246262E-4	0.0	0.0	7.7856325583355215	0.0
11	3.241312472246262E-4	0.0	0.0	9.363503469825002	0.0
12	3.241312472246262E-4	0.0	0.0	10.143039119400228	0.0
13	3.241312472246262E-4	0.0	0.0	10.509955691258504	0.0
14	3.241312472246262E-4	0.0	0.0	10.835707594719254	0.0
15	3.241312472246262E-4	0.0	0.0	11.109274367376837	0.0
16	3.241312472246262E-4	0.0	0.0	11.668076637592094	0.0
17	3.241312472246262E-4	0.0	0.0	12.402233912555872	0.0
18	3.241312472246262E-4	0.0	0.0	13.357448698126845	0.0
19	3.241312472246262E-4	0.0	0.0	13.76131623216873	0.0
20	3.241312472246262E-4	0.0	0.0	14.1992175471692	0.0
21	3.241312472246262E-4	0.0	0.0	14.635174074686322	0.0
22	3.241312472246262E-4	0.0	0.0	15.150866889020703	0.0
23	3.241312472246262E-4	0.0	0.0	15.6286363474298	0.0
24	3.241312472246262E-4	0.0	0.0	15.981939406904644	0.0
25	3.241312472246262E-4	0.0	0.0	16.291160616756937	0.0
26	3.241312472246262E-4	0.0	0.0	16.5605136832006	0.0
27	3.241312472246262E-4	0.0	0.0	16.917382186394914	0.0
28	3.241312472246262E-4	0.0	0.0	17.223037952527736	0.0
29	3.241312472246262E-4	0.0	0.0	17.567589468327515	0.0
30	3.241312472246262E-4	0.0	0.0	18.00646317706966	0.0
31	3.241312472246262E-4	0.0	0.0	18.35198708661111	0.0
32	3.241312472246262E-4	0.0	0.0	18.657318721496708	0.0
33	3.241312472246262E-4	0.0	0.0	18.999925449813137	0.0
34	3.241312472246262E-4	0.0	0.0	19.313036234632126	0.0
35	3.241312472246262E-4	0.0	0.0	19.67282191905146	0.0
36	3.241312472246262E-4	0.0	0.0	20.016076909862342	0.0
37	3.241312472246262E-4	0.0	0.0	20.35998016316767	0.0
38	3.241312472246262E-4	0.0	0.0	20.693835347809035	0.0
39	3.241312472246262E-4	0.0	0.0	21.034497288642118	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCAGTA	35	1.2080636E-7	45.000004	8
TAGACCG	35	1.2080636E-7	45.000004	1
CATTGGC	30	2.160321E-6	45.000004	43
GGACTAT	30	2.160321E-6	45.000004	8
TAGCGAG	35	1.2080636E-7	45.000004	1
TGCGAAG	30	2.160321E-6	45.000004	1
CGGGCCT	35	1.2080636E-7	45.000004	6
GGACGTA	30	2.160321E-6	45.000004	8
TTAACGG	35	1.2080636E-7	45.000004	2
ACTCGCG	35	1.2080636E-7	45.000004	1
CCGACGG	30	2.160321E-6	45.000004	2
TATACGG	30	2.160321E-6	45.000004	2
GCGTAGG	30	2.160321E-6	45.000004	2
GATAGCG	35	1.2080636E-7	45.000004	1
GTACGGG	70	0.0	45.000004	3
TACAACG	30	2.160321E-6	45.000004	1
CGGACAA	20	7.025255E-4	45.0	19
AACCGTC	40	6.7866495E-9	45.0	29
CTAGACG	25	3.8841506E-5	45.0	1
AACGTAG	45	3.8198777E-10	45.0	1
>>END_MODULE