SRR3553450_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553450_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	306793
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20381	6.643241534194065	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4897	1.5961902650973132	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3969	1.293706179736826	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2570	0.8376983829487634	No Hit
CTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGCT	1567	0.5107678467240127	TruSeq Adapter, Index 16 (95% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT	723	0.2356637863315004	No Hit
AAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	704	0.22947068544588697	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGT	613	0.1998089917305806	No Hit
AATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	578	0.18840064799392423	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCC	559	0.1822075471083108	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	503	0.16395419712966072	No Hit
AAGAGAGGGATATTGAAAATATTTTAATTTGCAGTGTCTTTTTTTTGTGAC	467	0.15221990071481423	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	433	0.14113750965634808	No Hit
TAGCTAGGAAAAAGAGGCCCAGACCAACTTTCGGTTAGAATTATGAATTAT	404	0.13168488198883288	No Hit
ACAATAGGGTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCAT	400	0.13038107127607215	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT	395	0.12875130788512124	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	381	0.12418797039045872	No Hit
GGAGGAGGGGAACAAATAATTATATTTAAAAATCTACTTTCAATTAGAAAA	376	0.12255820699950781	No Hit
GCTGAAGGGGGTTTGTAATAACTGTGGCACCTCAATATTTTTTATTTGCTT	366	0.119298680217606	No Hit
ATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTA	359	0.11701701147027474	No Hit
GGAAGCCGGGGAAATTGTATCATAATTTGCACTTTGAGAGGCTTGAGAGGC	355	0.11571320075751404	No Hit
AGATGAGGGGACGTAGGGGATGAGGAGAAAGGCGGAGGCAGCGAGAGTGAA	341	0.11114986326285149	No Hit
TTCCAAGGGCAATTTTTGATGTGGATTCATGCTACTTTTCTTTTATTATCT	333	0.10854224183733006	No Hit
GTCGTAGGGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	331	0.1078903364809497	No Hit
CACCTAGGGGTATTTCATGTGTACTTTTTCTGTTTCATATTGGATTTTTTA	320	0.10430485702085772	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT	308	0.10039342488257555	No Hit
TACCATGGGTCAAAGGGGACATTGGTGTTGATTATTAACAGGTAACTAAAT	307	0.10006747220438537	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGATA	35	1.2080272E-7	45.000004	41
CCTTATT	35	1.2080272E-7	45.000004	20
CGAACAT	35	1.2080272E-7	45.000004	2
CGAACAG	35	1.2080272E-7	45.000004	1
CTCGTAG	35	1.2080272E-7	45.000004	38
CAAAAGT	35	1.2080272E-7	45.000004	18
GCGCGTC	35	1.2080272E-7	45.000004	22
CTATAGT	35	1.2080272E-7	45.000004	35
GGTCCGA	35	1.2080272E-7	45.000004	11
TGTATGC	35	1.2080272E-7	45.000004	45
ACGGGTC	35	1.2080272E-7	45.000004	5
ATCGTGT	35	1.2080272E-7	45.000004	26
ATGTAGT	35	1.2080272E-7	45.000004	25
CAGGGGT	130	0.0	45.000004	5
CATAGTA	35	1.2080272E-7	45.000004	9
CGTAGCC	35	1.2080272E-7	45.000004	40
CGCTAGC	35	1.2080272E-7	45.000004	28
ACTGTGC	35	1.2080272E-7	45.000004	9
AGTTCGT	35	1.2080272E-7	45.000004	39
TCACTAT	35	1.2080272E-7	45.000004	37

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content