##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553450_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	306793
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.494763570224876	31.0	31.0	34.0	30.0	34.0
2	31.661771943949176	31.0	31.0	34.0	30.0	34.0
3	31.889003334495897	33.0	31.0	34.0	30.0	34.0
4	35.64207788313293	37.0	35.0	37.0	33.0	37.0
5	35.364640001564574	37.0	35.0	37.0	33.0	37.0
6	34.03155221924881	37.0	35.0	37.0	31.0	37.0
7	34.734896167774366	37.0	35.0	37.0	32.0	37.0
8	35.33857682541649	37.0	35.0	37.0	32.0	37.0
9	36.91040538734587	39.0	37.0	39.0	32.0	39.0
10	36.58684194228682	38.0	35.0	39.0	32.0	39.0
11	36.5945539826528	38.0	35.0	39.0	32.0	39.0
12	36.478896193850574	38.0	35.0	39.0	32.0	39.0
13	36.404135687580876	38.0	35.0	39.0	32.0	39.0
14	37.50311447784011	39.0	36.0	41.0	32.0	41.0
15	37.5606581636478	39.0	36.0	41.0	32.0	41.0
16	37.4503459987679	39.0	36.0	41.0	32.0	41.0
17	37.31007878276232	39.0	36.0	40.0	32.0	41.0
18	37.006105093662505	38.0	36.0	40.0	32.0	41.0
19	36.64794503134035	38.0	35.0	40.0	31.0	41.0
20	36.51753136479646	38.0	35.0	40.0	31.0	41.0
21	36.41713142086032	38.0	35.0	40.0	31.0	41.0
22	36.3960618397421	38.0	35.0	40.0	31.0	41.0
23	36.070435114230115	38.0	35.0	40.0	30.0	41.0
24	35.87264051005075	38.0	34.0	40.0	30.0	41.0
25	35.903042116345546	38.0	34.0	40.0	30.0	41.0
26	35.85983056979788	38.0	34.0	40.0	30.0	41.0
27	35.73459955083721	38.0	34.0	40.0	30.0	41.0
28	35.56319081595734	37.0	34.0	40.0	29.0	41.0
29	35.35501461897762	37.0	34.0	40.0	27.0	41.0
30	35.28560299615702	37.0	34.0	40.0	27.0	41.0
31	35.15382032836473	37.0	34.0	40.0	26.0	41.0
32	34.99879723461748	37.0	34.0	40.0	25.0	41.0
33	34.87923779225732	37.0	34.0	40.0	25.0	41.0
34	34.71761415677672	37.0	33.0	40.0	25.0	41.0
35	34.5606223088532	37.0	33.0	40.0	24.0	41.0
36	34.46917628498694	37.0	33.0	40.0	24.0	41.0
37	34.32302888266681	37.0	33.0	40.0	23.0	41.0
38	34.22738458830547	36.0	33.0	40.0	23.0	41.0
39	34.07975084177279	36.0	33.0	40.0	22.0	41.0
40	33.843871926673685	36.0	33.0	40.0	21.0	41.0
41	33.657941999980444	36.0	33.0	40.0	21.0	41.0
42	33.48024563793828	35.0	33.0	40.0	20.0	41.0
43	33.43855303087098	35.0	33.0	40.0	20.0	41.0
44	33.258754274054496	35.0	33.0	40.0	19.0	41.0
45	33.08723145573725	35.0	33.0	39.0	20.0	41.0
46	32.86344864452579	35.0	32.0	39.0	19.0	41.0
47	32.750792879889694	35.0	32.0	39.0	18.0	41.0
48	32.50990407212681	35.0	31.0	39.0	17.0	41.0
49	32.365399471304755	35.0	31.0	39.0	15.0	41.0
50	32.2364558513395	35.0	31.0	39.0	15.0	40.0
51	31.05835856750317	35.0	28.0	38.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	2.0
14	9.0
15	44.0
16	96.0
17	194.0
18	448.0
19	851.0
20	1360.0
21	2008.0
22	2666.0
23	3474.0
24	4024.0
25	4407.0
26	4716.0
27	5107.0
28	5830.0
29	6974.0
30	8415.0
31	10401.0
32	13329.0
33	17402.0
34	25227.0
35	38969.0
36	30442.0
37	36386.0
38	45865.0
39	38140.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.67583028295952	37.074509522707494	18.499118298005495	23.75054189632749
2	18.30517645448234	42.45338061820185	16.361194681756103	22.88024824555971
3	17.703141857865077	43.5736799731415	17.313954360106	21.409223808887425
4	17.40587301535563	42.30442024426894	16.24939291313687	24.04031382723856
5	15.99775744557405	43.56553115618674	15.675716199522153	24.76099519871705
6	15.610199711205928	50.14195239135182	16.470388828949815	17.777459068492433
7	68.73201148657238	22.054936064382172	5.142229451128285	4.070822997917163
8	70.244757866053	20.65268764280802	4.332562998503877	4.769991492635099
9	64.38054323273347	23.792589791814027	7.82449403995528	4.002372935497225
10	29.87812629362469	43.78326754521778	11.546547672208948	14.792058488948575
11	22.892634447330938	40.777983852304324	21.41443905173847	14.914942648626273
12	22.772683861756953	34.567933427425004	25.911282200050195	16.748100510767845
13	21.680416437141655	37.10580097981375	24.03216501028381	17.18161757276079
14	16.237006711365645	39.82424631591985	24.5967150489092	19.3420319238053
15	15.901927358186137	39.98494098626762	25.59152262274563	18.52160903280062
16	17.270602653906707	39.491774584165874	23.611686055418474	19.62593670650895
17	16.525148878885762	40.0615398656423	24.223173279703254	19.19013797576868
18	18.6516641513985	38.9529748071175	21.441493124028256	20.953867917455742
19	16.853383225823276	43.83053068355537	21.26710844119651	18.048977649424856
20	19.88246146424462	39.16517000061931	22.42978164430088	18.52258689083519
21	18.517371647984145	41.19063994289309	21.61620375953819	18.675784649584575
22	18.991632794750856	38.50772344870972	20.83978448008918	21.660859276450246
23	19.06203857323994	38.69840576545097	21.391948316943346	20.847607344365745
24	15.401915949842401	41.1736904036272	22.203570485636895	21.220823160893502
25	17.797016229183846	41.71607566013566	21.010257730782644	19.476650379897848
26	19.349202882725486	40.2541778984527	20.08259640865339	20.31402281016842
27	17.034938867575207	40.13683493430424	21.102828291388658	21.7253979067319
28	17.342638195786737	40.47354405087469	22.457813574625234	19.726004178713335
29	19.215562284667513	41.2069375768026	18.983483977796105	20.594016160733787
30	18.20087159746148	40.511680514222945	21.255374144781662	20.032073743533914
31	19.022598299178924	40.23396883240491	18.64547105051289	22.09796181790328
32	20.118777155932502	38.346702825683764	20.25763299684152	21.276887021542212
33	18.150022979663813	37.20293487791442	21.313067768821323	23.33397437360044
34	18.22108066350927	38.023357768919105	22.280821270367966	21.474740297203653
35	18.807143578895218	36.04384715427014	22.17912403477263	22.96988523206201
36	19.709380592125637	36.895235549702896	23.22934356390139	20.166040294270076
37	20.41897957254566	35.790255970638185	22.197051432073092	21.593713024743067
38	20.498512026024063	35.234180701645734	23.72739925617599	20.539908016154214
39	21.21951935018074	33.54020463309137	23.324521745932923	21.915754270794967
40	21.244943659079574	34.87595870831473	23.805953851619822	20.073143780985877
41	18.911122483237882	32.50400106912478	27.87090970132956	20.713966746307772
42	20.791869436395224	34.4088685204682	25.138448400061282	19.660813643075297
43	18.660790826387824	32.85440019817923	26.274067530875868	22.21074144455708
44	20.96299459244507	32.789535615219386	23.834637687300557	22.41283210503499
45	20.89324071931237	32.89090689813653	24.77533711655742	21.440515265993685
46	18.79606118783675	33.626582092811766	26.979103173801228	20.598253545550257
47	19.572154514607572	32.70185434478623	25.563164739743083	22.16282640086312
48	18.080269106531112	32.783994419690146	28.450779515829893	20.684956957948845
49	19.344639545230823	32.59787544044356	25.73559370650569	22.32189130781993
50	19.28401234708745	31.630447891575102	25.128995772393765	23.956543988943686
51	18.186529679621113	31.733448937883196	26.86990902660752	23.21011235588817
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23746.0
1	17849.5
2	11953.0
3	7370.5
4	2788.0
5	2263.5
6	1739.0
7	1795.0
8	1851.0
9	1996.5
10	2142.0
11	2347.5
12	2553.0
13	2603.0
14	2653.0
15	2971.0
16	3289.0
17	2892.5
18	2496.0
19	2505.0
20	2514.0
21	2281.0
22	2048.0
23	2059.5
24	2071.0
25	2253.5
26	2626.5
27	2817.0
28	3391.5
29	3966.0
30	4140.5
31	4315.0
32	5158.5
33	6002.0
34	5785.5
35	5569.0
36	6581.5
37	7594.0
38	7014.0
39	6434.0
40	6916.0
41	7398.0
42	9256.0
43	11114.0
44	14124.5
45	17135.0
46	18918.0
47	20701.0
48	22432.5
49	24164.0
50	23288.0
51	22412.0
52	20214.5
53	18017.0
54	16018.0
55	14019.0
56	13246.0
57	12473.0
58	11662.5
59	10852.0
60	10705.0
61	10558.0
62	9952.5
63	9347.0
64	7739.5
65	6132.0
66	6116.5
67	6101.0
68	5554.0
69	5007.0
70	4317.5
71	3628.0
72	3397.0
73	3166.0
74	2236.5
75	1134.0
76	961.0
77	799.5
78	638.0
79	425.5
80	213.0
81	219.5
82	226.0
83	200.5
84	175.0
85	109.0
86	43.0
87	27.0
88	11.0
89	9.5
90	8.0
91	8.5
92	9.0
93	5.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	306793.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.92343371589313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.05290676775269	24.253812831453132
2	8.268792958835768	4.948613560283318
3	2.983562628672266	2.678353156688712
4	1.5936298378048646	1.9074750727689356
5	0.9672886506976897	1.4472298911644008
6	0.6274304761282311	1.1264924558252631
7	0.48255503632778884	1.0107792550677492
8	0.3703582671590253	0.8865912846772905
9	0.3169831051272834	0.8536700641800824
>10	2.3125605917017964	15.113447829644091
>50	0.5642517129069856	12.268858807078388
>100	0.4476977876539982	21.423565726727794
>500	0.006535734126335741	1.1995058557398637
>1k	0.004357156084223827	4.238362674506916
>5k	0.0	0.0
>10k+	0.0010892890210559567	6.643241534194065
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20381	6.643241534194065	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4897	1.5961902650973132	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3969	1.293706179736826	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2570	0.8376983829487634	No Hit
CTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGCT	1567	0.5107678467240127	TruSeq Adapter, Index 16 (95% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT	723	0.2356637863315004	No Hit
AAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	704	0.22947068544588697	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGT	613	0.1998089917305806	No Hit
AATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	578	0.18840064799392423	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCC	559	0.1822075471083108	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	503	0.16395419712966072	No Hit
AAGAGAGGGATATTGAAAATATTTTAATTTGCAGTGTCTTTTTTTTGTGAC	467	0.15221990071481423	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	433	0.14113750965634808	No Hit
TAGCTAGGAAAAAGAGGCCCAGACCAACTTTCGGTTAGAATTATGAATTAT	404	0.13168488198883288	No Hit
ACAATAGGGTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCAT	400	0.13038107127607215	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT	395	0.12875130788512124	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	381	0.12418797039045872	No Hit
GGAGGAGGGGAACAAATAATTATATTTAAAAATCTACTTTCAATTAGAAAA	376	0.12255820699950781	No Hit
GCTGAAGGGGGTTTGTAATAACTGTGGCACCTCAATATTTTTTATTTGCTT	366	0.119298680217606	No Hit
ATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTA	359	0.11701701147027474	No Hit
GGAAGCCGGGGAAATTGTATCATAATTTGCACTTTGAGAGGCTTGAGAGGC	355	0.11571320075751404	No Hit
AGATGAGGGGACGTAGGGGATGAGGAGAAAGGCGGAGGCAGCGAGAGTGAA	341	0.11114986326285149	No Hit
TTCCAAGGGCAATTTTTGATGTGGATTCATGCTACTTTTCTTTTATTATCT	333	0.10854224183733006	No Hit
GTCGTAGGGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	331	0.1078903364809497	No Hit
CACCTAGGGGTATTTCATGTGTACTTTTTCTGTTTCATATTGGATTTTTTA	320	0.10430485702085772	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT	308	0.10039342488257555	No Hit
TACCATGGGTCAAAGGGGACATTGGTGTTGATTATTAACAGGTAACTAAAT	307	0.10006747220438537	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2595267819018035E-4	0.0	0.0	0.6268070001597168	0.0
2	3.2595267819018035E-4	0.0	0.0	0.8497586320418001	0.0
3	3.2595267819018035E-4	0.0	0.0	1.1248626924343124	0.0
4	3.2595267819018035E-4	0.0	0.0	2.5163546756281923	0.0
5	3.2595267819018035E-4	0.0	0.0	2.7588634682016866	0.0
6	3.2595267819018035E-4	0.0	0.0	4.063977991675168	0.0
7	3.2595267819018035E-4	0.0	0.0	5.018367433416016	0.0
8	3.2595267819018035E-4	0.0	0.0	5.784356227162941	0.0
9	3.2595267819018035E-4	0.0	0.0	6.878253415169186	0.0
10	3.2595267819018035E-4	0.0	0.0	8.731294390680361	0.0
11	3.2595267819018035E-4	0.0	0.0	10.141039723852892	0.0
12	3.2595267819018035E-4	0.0	0.0	10.926259725613036	0.0
13	3.2595267819018035E-4	0.0	0.0	11.252538356481406	0.0
14	3.2595267819018035E-4	0.0	0.0	11.536443139185053	0.0
15	3.2595267819018035E-4	0.0	0.0	11.781233600505878	0.0
16	3.2595267819018035E-4	0.0	0.0	12.282222866884187	0.0
17	3.2595267819018035E-4	0.0	0.0	12.90316271883648	0.0
18	3.2595267819018035E-4	0.0	0.0	14.038129944294687	0.0
19	3.2595267819018035E-4	0.0	0.0	14.328879733240328	0.0
20	3.2595267819018035E-4	0.0	0.0	14.830520904975016	0.0
21	3.2595267819018035E-4	0.0	0.0	15.14506523942854	0.0
22	3.2595267819018035E-4	0.0	0.0	15.475907207791574	0.0
23	3.2595267819018035E-4	0.0	0.0	15.792081305636048	0.0
24	3.2595267819018035E-4	0.0	0.0	16.01535889019632	0.0
25	3.2595267819018035E-4	0.0	0.0	16.214841929248713	0.0
26	3.2595267819018035E-4	0.0	0.0	16.397049476357022	0.0
27	3.2595267819018035E-4	0.0	0.0	16.647707085885273	0.0
28	3.2595267819018035E-4	0.0	0.0	16.869028954376404	0.0
29	3.2595267819018035E-4	0.0	0.0	17.096869876431338	0.0
30	3.2595267819018035E-4	0.0	0.0	17.44140185727836	0.0
31	3.2595267819018035E-4	0.0	0.0	17.655552766849308	0.0
32	3.2595267819018035E-4	0.0	0.0	17.861554859465503	0.0
33	3.2595267819018035E-4	0.0	0.0	18.088417923485867	0.0
34	3.2595267819018035E-4	0.0	0.0	18.279100240227123	0.0
35	3.2595267819018035E-4	0.0	0.0	18.464567314117335	0.0
36	3.2595267819018035E-4	0.0	0.0	18.65459772550221	0.0
37	3.2595267819018035E-4	0.0	0.0	18.85245100116365	0.0
38	3.2595267819018035E-4	0.0	0.0	19.062690478596316	0.0
39	3.2595267819018035E-4	0.0	0.0	19.263477328361468	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGATA	35	1.2080272E-7	45.000004	41
CCTTATT	35	1.2080272E-7	45.000004	20
CGAACAT	35	1.2080272E-7	45.000004	2
CGAACAG	35	1.2080272E-7	45.000004	1
CTCGTAG	35	1.2080272E-7	45.000004	38
CAAAAGT	35	1.2080272E-7	45.000004	18
GCGCGTC	35	1.2080272E-7	45.000004	22
CTATAGT	35	1.2080272E-7	45.000004	35
GGTCCGA	35	1.2080272E-7	45.000004	11
TGTATGC	35	1.2080272E-7	45.000004	45
ACGGGTC	35	1.2080272E-7	45.000004	5
ATCGTGT	35	1.2080272E-7	45.000004	26
ATGTAGT	35	1.2080272E-7	45.000004	25
CAGGGGT	130	0.0	45.000004	5
CATAGTA	35	1.2080272E-7	45.000004	9
CGTAGCC	35	1.2080272E-7	45.000004	40
CGCTAGC	35	1.2080272E-7	45.000004	28
ACTGTGC	35	1.2080272E-7	45.000004	9
AGTTCGT	35	1.2080272E-7	45.000004	39
TCACTAT	35	1.2080272E-7	45.000004	37
>>END_MODULE