FastQCFastQC Report
Sat 18 Jun 2016
SRR3553447_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553447_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010428
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA137971.365460972973829No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34430.34074669348038655No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33090.32748498656015074No Hit
CTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGCT24470.24217460323743997Illumina Single End Adapter 1 (95% over 21bp)
AGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCAGTGCTGATC16000.1583487393460989No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15630.15468692474872034No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGT10760.1064895272102515No Hit
AGCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCTGA10750.1063905592481602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG351.211738E-745.0000041
CTAACCG351.211738E-745.0000041
ATACGAG351.211738E-745.0000041
AATCGCC351.211738E-745.00000424
TCCGCAT253.8904698E-545.035
CAAGTCG253.8904698E-545.09
GATCGAT207.0328807E-445.09
CGTTGTA207.0328807E-445.013
ATAGCGA207.0328807E-445.043
TATGCGT253.8904698E-545.018
ATTTTCG207.0328807E-445.044
TAACACG502.1827873E-1145.01
AACGCAC207.0328807E-445.031
TCGTCGT207.0328807E-445.039
GTCGTAC207.0328807E-445.020
TACCTCG207.0328807E-445.017
CGTACTA207.0328807E-445.038
TCCCGTT207.0328807E-445.042
TATAGCG207.0328807E-445.01
CGTCGGA207.0328807E-445.027