FastQCFastQC Report
Sat 18 Jun 2016
SRR3553444_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553444_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734355
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180772.4616159759244507No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT70810.964247536954198No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45170.615097602658115No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37830.5151459444001879No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGT35020.4768810725058044No Hit
AATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT28280.3850998495278169No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20910.2847396695058929No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCC18690.2545090589701166No Hit
AAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT15070.20521409944781474No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT13610.1853327069332952No Hit
AATGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT9940.1353568778043317No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC8830.12024157253644355No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT8030.10934765882985749No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7850.10689652824587563No Hit
ACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG7800.106215658639214No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG351.2111195E-745.0000041
CGGGTCG351.2111195E-745.0000046
CGGGTAG351.2111195E-745.0000046
ACACGTA650.045.00000427
ATCTACG650.045.0000041
TAGCGAT351.2111195E-745.00000445
TCGGCCG351.2111195E-745.00000413
CGTTAGC351.2111195E-745.00000432
TACGCGG750.045.0000042
GTCACTA351.2111195E-745.00000423
TATTCGG351.2111195E-745.0000042
TTGACGT351.2111195E-745.00000429
CTCGCTA351.2111195E-745.00000420
CCGGTCG351.2111195E-745.0000041
CAGTCAA351.2111195E-745.00000414
CACGTAC650.045.00000428
CCGCACG351.2111195E-745.0000041
CGGACGT351.2111195E-745.00000428
TCCGGAC253.8894246E-545.04
ATTTACG253.8894246E-545.01