##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553444_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	734355
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.261429417652227	31.0	31.0	34.0	28.0	34.0
2	31.47180995567539	31.0	31.0	34.0	28.0	34.0
3	31.670309319062305	31.0	31.0	34.0	30.0	34.0
4	35.446463903697804	37.0	35.0	37.0	33.0	37.0
5	35.149408664746616	37.0	35.0	37.0	32.0	37.0
6	33.825115918050535	36.0	35.0	37.0	30.0	37.0
7	34.723644558830536	37.0	35.0	37.0	32.0	37.0
8	35.42277645008205	37.0	35.0	37.0	32.0	37.0
9	37.05076019091584	39.0	37.0	39.0	33.0	39.0
10	36.297623084203146	38.0	35.0	39.0	32.0	39.0
11	36.41233871901192	38.0	35.0	39.0	32.0	39.0
12	36.50674673693241	38.0	35.0	39.0	32.0	39.0
13	36.480248653580354	38.0	35.0	39.0	32.0	39.0
14	37.5475880194184	39.0	36.0	40.0	32.0	41.0
15	37.51261583294183	39.0	36.0	40.0	32.0	41.0
16	37.4132361051535	39.0	36.0	40.0	32.0	41.0
17	37.3712904521655	39.0	36.0	40.0	32.0	41.0
18	37.24886328819168	39.0	36.0	40.0	32.0	41.0
19	37.16924239638867	39.0	35.0	40.0	32.0	41.0
20	37.07981289703209	39.0	35.0	40.0	31.0	41.0
21	37.009841289294684	39.0	35.0	40.0	31.0	41.0
22	37.016575089704574	39.0	35.0	40.0	31.0	41.0
23	36.82947484527238	39.0	35.0	40.0	31.0	41.0
24	36.63395496728422	38.0	35.0	40.0	31.0	41.0
25	36.75271360581735	39.0	35.0	40.0	31.0	41.0
26	36.61886281158295	38.0	35.0	40.0	31.0	41.0
27	36.61383390866815	39.0	35.0	40.0	31.0	41.0
28	36.56477180655133	38.0	35.0	40.0	31.0	41.0
29	36.37040668341606	38.0	35.0	40.0	30.0	41.0
30	36.371155639983385	38.0	35.0	40.0	30.0	41.0
31	36.29201271864425	38.0	35.0	40.0	30.0	41.0
32	36.1866004861409	38.0	35.0	40.0	30.0	41.0
33	36.1713517304301	38.0	35.0	40.0	30.0	41.0
34	36.098535449476074	38.0	35.0	40.0	30.0	41.0
35	35.99932185387176	38.0	35.0	40.0	30.0	41.0
36	35.957358498274	38.0	35.0	40.0	30.0	41.0
37	35.84568226538936	38.0	35.0	40.0	29.0	41.0
38	35.784349531221274	38.0	35.0	40.0	29.0	41.0
39	35.69623547194477	38.0	35.0	40.0	29.0	41.0
40	35.54775687508085	38.0	34.0	40.0	28.0	41.0
41	35.52774747908028	38.0	34.0	40.0	28.0	41.0
42	35.41575532269815	38.0	34.0	40.0	27.0	41.0
43	35.341737987757966	38.0	34.0	40.0	27.0	41.0
44	35.19210191256273	38.0	34.0	40.0	26.0	41.0
45	35.01298963035589	38.0	34.0	40.0	26.0	41.0
46	34.84535817145658	37.0	33.0	40.0	25.0	41.0
47	34.80709193782299	37.0	33.0	40.0	25.0	41.0
48	34.65301795453153	37.0	33.0	40.0	24.0	41.0
49	34.486195368724935	37.0	33.0	40.0	24.0	41.0
50	34.389040722811174	37.0	33.0	40.0	24.0	41.0
51	33.14378059657795	36.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	6.0
12	5.0
13	4.0
14	15.0
15	40.0
16	106.0
17	222.0
18	436.0
19	801.0
20	1458.0
21	2239.0
22	2939.0
23	3923.0
24	4907.0
25	5792.0
26	6955.0
27	8467.0
28	10892.0
29	13666.0
30	17732.0
31	22960.0
32	29657.0
33	38713.0
34	53547.0
35	76103.0
36	82692.0
37	103973.0
38	133619.0
39	112470.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.76857922939178	34.0000408521764	20.616323167950107	26.615056750481713
2	20.438207678847423	34.65149689183025	21.00441884374723	23.905876585575097
3	19.247911432481565	34.32604121984599	22.54577145930783	23.88027588836462
4	18.559143738382662	35.05933778622056	20.39790019813306	25.98361827726372
5	16.2701962947076	38.98373402509686	18.37231311831471	26.373756561880835
6	17.81182125811086	40.66425638825908	19.559068842725928	21.964853510904124
7	77.96419987608174	10.796957874597437	6.225463161549931	5.0133790877709
8	79.89868660252874	9.099005249504668	4.981650564100469	6.020657583866114
9	72.62482042064124	13.703454051514594	9.171722123496128	4.500003404348033
10	25.57264538268276	46.65604510080274	13.094620449237768	14.676689067276726
11	16.687297015748513	31.74636245412641	34.09985633651299	17.466484193612082
12	20.3829210667865	27.21871574374791	33.734093183814366	18.66427000565122
13	21.246944597640105	28.300617548733243	29.536123536981435	20.916314316645217
14	16.39888065036665	31.261174772419338	31.827249763397813	20.512694813816207
15	14.74436750617889	34.76942350770404	28.915034281784695	21.571174704332375
16	16.61158431548774	33.519755431637286	28.397709554643193	21.470950698231782
17	15.280075712700262	33.28921298282166	29.693132068277606	21.737579236200474
18	15.753960958936755	32.58669172266819	27.960999788930422	23.698347529464634
19	16.635278577799564	36.48725752531133	29.031871506287832	17.845592390601276
20	17.969238311171026	34.22704277903739	28.122638233551893	19.68108067623969
21	17.492765760429222	35.08589170088036	26.92458007367009	20.496762465020325
22	16.929958943562717	32.44629640977457	28.23416467512307	22.38957997153965
23	17.98203865977627	34.158547296607225	25.64522608275289	22.214187960863615
24	13.916430064478352	35.81469452785097	27.336097663936382	22.9327777437343
25	17.36803044848881	33.462698558599044	25.984435320791714	23.184835672120432
26	18.344261290520254	34.482777403299494	24.650747935262917	22.522213370917335
27	14.83437846817956	33.77562622982072	26.537165267479622	24.85283003452009
28	17.875959175058384	32.15924178360602	26.909464768402202	23.05533427293339
29	18.028201619107925	31.947355162012926	26.7881338044951	23.236309414384053
30	17.67033655384657	31.039211280647645	28.785532882597654	22.50491928290813
31	19.22244690919242	32.69808199031804	23.26204628551586	24.817424814973684
32	18.04086579379183	30.25226218926813	28.124408494529213	23.582463522410823
33	18.15021345262169	30.187579576635276	27.875618740255053	23.78658823048798
34	16.007108278693547	29.115073772221884	27.39955471127724	27.478263237807326
35	16.387033519210735	28.314234940866474	30.713483260820716	24.58524827910207
36	17.823940737109435	29.784913291255595	29.64792232639527	22.7432236452397
37	17.535660545648906	31.297397035493734	29.026016027670543	22.140926391186824
38	19.015054027003288	29.609929802343554	32.35151936052726	19.02349681012589
39	17.699069251247693	29.14012977374703	30.63981316937993	22.520987805625346
40	18.332141811521677	29.265001259608773	28.7212587917288	23.681598137140757
41	16.323712645791204	27.640582552035458	31.754941411170346	24.28076339100299
42	19.198480299037932	29.227825779085048	26.959985293216498	24.613708628660525
43	16.353943256326982	29.266090650979432	29.368084918057342	25.011881174636247
44	19.605640323821586	28.09894397124007	27.572223243526633	24.723192461411713
45	20.583641426830347	29.60203171490628	27.986055790455573	21.828271067807805
46	16.700097364353752	28.72779513995275	32.13078143404757	22.441326061645935
47	17.900606654819534	28.470017906870655	28.883305758114265	24.746069680195546
48	17.855533086858536	27.20659626474934	32.25864874617862	22.679221902213506
49	18.665359397021877	27.20482600377202	30.26152201591873	23.868292583287378
50	18.208632064873257	26.37988438834079	28.06135996895235	27.350123577833607
51	17.768381777205846	25.364163109122973	32.4232830170694	24.44417209660178
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20753.0
1	16244.0
2	11735.0
3	7341.5
4	2948.0
5	2400.0
6	1852.0
7	2032.5
8	2213.0
9	2612.5
10	3012.0
11	3947.5
12	4883.0
13	4973.5
14	5064.0
15	5293.5
16	5523.0
17	5311.0
18	5099.0
19	5198.0
20	5297.0
21	4953.0
22	4609.0
23	4524.5
24	4440.0
25	5888.0
26	7768.5
27	8201.0
28	9825.0
29	11449.0
30	13863.5
31	16278.0
32	16532.5
33	16787.0
34	20161.0
35	23535.0
36	25013.5
37	26492.0
38	27538.0
39	28584.0
40	30021.5
41	31459.0
42	35651.5
43	39844.0
44	51215.5
45	62587.0
46	66080.0
47	69573.0
48	75104.5
49	80636.0
50	72072.0
51	63508.0
52	54711.0
53	45914.0
54	40776.5
55	35639.0
56	29156.0
57	22673.0
58	21109.5
59	19546.0
60	16621.5
61	13697.0
62	11955.0
63	10213.0
64	8808.5
65	7404.0
66	6398.5
67	5393.0
68	4255.5
69	3118.0
70	2803.5
71	2489.0
72	2162.0
73	1835.0
74	1509.0
75	897.0
76	611.0
77	589.0
78	567.0
79	403.5
80	240.0
81	152.0
82	64.0
83	50.5
84	37.0
85	28.0
86	19.0
87	11.5
88	4.0
89	5.5
90	7.0
91	5.5
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	734355.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.20407495533498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.4155443554496	21.732371351986053
2	10.581229123173747	6.1803001686547905
3	4.26528649397189	3.7369123942779905
4	2.2852727560057997	2.6695710743911327
5	1.415293429229598	2.066616769550713
6	0.9717528669416775	1.7027486138535848
7	0.7043304088614818	1.4398522637598712
8	0.5436620562944652	1.2701717953916085
9	0.424427508850482	1.1155511503418019
>10	3.4231324624371395	20.60476441476581
>50	0.5942976209701235	12.322281501756597
>100	0.3653196407066509	17.477306538975828
>500	0.005700696604004955	1.2142201772503787
>1k	0.00380046440266997	2.977003720685401
>5k	4.750580503337463E-4	0.9823918047429081
>10k+	4.750580503337463E-4	2.5079362596155277
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18077	2.4616159759244507	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT	7081	0.964247536954198	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4517	0.615097602658115	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3783	0.5151459444001879	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGT	3502	0.4768810725058044	No Hit
AATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	2828	0.3850998495278169	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2091	0.2847396695058929	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCC	1869	0.2545090589701166	No Hit
AAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	1507	0.20521409944781474	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT	1361	0.1853327069332952	No Hit
AATGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT	994	0.1353568778043317	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	883	0.12024157253644355	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT	803	0.10934765882985749	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	785	0.10689652824587563	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	780	0.106215658639214	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3617392133232566E-4	0.0	0.0	1.1384139823382424	0.0
2	1.3617392133232566E-4	0.0	0.0	1.4921938299596245	0.0
3	1.3617392133232566E-4	0.0	0.0	1.973296294026731	0.0
4	1.3617392133232566E-4	0.0	0.0	4.146632078490648	0.0
5	1.3617392133232566E-4	0.0	0.0	4.453431923252378	0.0
6	1.3617392133232566E-4	0.0	0.0	6.279796556161529	0.0
7	1.3617392133232566E-4	0.0	0.0	7.795820822354311	0.0
8	1.3617392133232566E-4	0.0	0.0	8.839049233681258	0.0
9	1.3617392133232566E-4	0.0	0.0	10.731866740200584	0.0
10	1.3617392133232566E-4	0.0	0.0	13.019043922898327	0.0
11	1.3617392133232566E-4	0.0	0.0	15.183800750318307	0.0
12	1.3617392133232566E-4	0.0	0.0	16.299746035636716	0.0
13	1.3617392133232566E-4	0.0	0.0	16.752932845830696	0.0
14	1.3617392133232566E-4	0.0	0.0	17.208570786608657	0.0
15	1.3617392133232566E-4	0.0	0.0	17.579780896160575	0.0
16	1.3617392133232566E-4	0.0	0.0	18.31430302782714	0.0
17	1.3617392133232566E-4	0.0	0.0	19.365565700512693	0.0
18	1.3617392133232566E-4	0.0	0.0	20.701568042704142	0.0
19	1.3617392133232566E-4	0.0	0.0	21.27758372993988	0.0
20	1.3617392133232566E-4	0.0	0.0	21.82513906761716	0.0
21	1.3617392133232566E-4	0.0	0.0	22.43683232224197	0.0
22	1.3617392133232566E-4	0.0	0.0	23.14248558258608	0.0
23	1.3617392133232566E-4	0.0	0.0	23.829483015707662	0.0
24	1.3617392133232566E-4	0.0	0.0	24.32188791524535	0.0
25	1.3617392133232566E-4	0.0	0.0	24.729456461793003	0.0
26	1.3617392133232566E-4	0.0	0.0	25.11932239856745	0.0
27	1.3617392133232566E-4	0.0	0.0	25.53560607608037	0.0
28	1.3617392133232566E-4	0.0	0.0	25.99805271292495	0.0
29	1.3617392133232566E-4	0.0	0.0	26.44851604469228	0.0
30	1.3617392133232566E-4	0.0	0.0	27.045638689734528	0.0
31	1.3617392133232566E-4	0.0	0.0	27.482348455447298	0.0
32	1.3617392133232566E-4	0.0	0.0	27.880521001423016	0.0
33	1.3617392133232566E-4	0.0	0.0	28.302660157553227	0.0
34	1.3617392133232566E-4	0.0	0.0	28.712816008606193	0.0
35	1.3617392133232566E-4	0.0	0.0	29.170224210361475	0.0
36	1.3617392133232566E-4	0.0	0.0	29.594269801390336	0.0
37	1.3617392133232566E-4	0.0	0.0	30.039830871989704	0.0
38	1.3617392133232566E-4	0.0	0.0	30.427926547786832	0.0
39	1.3617392133232566E-4	0.0	0.0	30.849521008231715	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	35	1.2111195E-7	45.000004	1
CGGGTCG	35	1.2111195E-7	45.000004	6
CGGGTAG	35	1.2111195E-7	45.000004	6
ACACGTA	65	0.0	45.000004	27
ATCTACG	65	0.0	45.000004	1
TAGCGAT	35	1.2111195E-7	45.000004	45
TCGGCCG	35	1.2111195E-7	45.000004	13
CGTTAGC	35	1.2111195E-7	45.000004	32
TACGCGG	75	0.0	45.000004	2
GTCACTA	35	1.2111195E-7	45.000004	23
TATTCGG	35	1.2111195E-7	45.000004	2
TTGACGT	35	1.2111195E-7	45.000004	29
CTCGCTA	35	1.2111195E-7	45.000004	20
CCGGTCG	35	1.2111195E-7	45.000004	1
CAGTCAA	35	1.2111195E-7	45.000004	14
CACGTAC	65	0.0	45.000004	28
CCGCACG	35	1.2111195E-7	45.000004	1
CGGACGT	35	1.2111195E-7	45.000004	28
TCCGGAC	25	3.8894246E-5	45.0	4
ATTTACG	25	3.8894246E-5	45.0	1
>>END_MODULE