FastQCFastQC Report
Sat 18 Jun 2016
SRR3553443_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553443_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720886
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA179142.4849976279189776No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT67030.9298280171899579No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45820.6356067394844677No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36600.5077085697322461No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGT29730.4124091742661114No Hit
AATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT25970.36025113540837245No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20770.2881176774136271No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCC17470.2423406752246541No Hit
AAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT13770.19101494549762377No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT12690.17603338114486894No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC8880.12318175134487283No Hit
AATGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT8680.12040738757584417No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7740.10736787786140944No Hit
ACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG7590.10528710503463794No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT7270.10084812300419208No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAC207.031533E-445.0000044
CGTATAA207.031533E-445.00000426
CAACGTA207.031533E-445.00000444
CTCCGCA207.031533E-445.00000444
CGCATTC207.031533E-445.00000414
CTCCCGG750.045.0000042
AGGCGTC207.031533E-445.00000432
GCGTTTC207.031533E-445.00000429
CCGCTTC207.031533E-445.00000439
CCCACCG207.031533E-445.0000041
CGTTACT207.031533E-445.00000429
GTTACGT207.031533E-445.00000431
TACGCGT207.031533E-445.00000422
CTAGTCG207.031533E-445.00000437
TACGAAG551.8189894E-1245.0000041
ACGTGTT207.031533E-445.00000445
TGTTCCG207.031533E-445.0000041
GACGGTG207.031533E-445.00000426
GTCCGCC207.031533E-445.00000421
AACGACC207.031533E-445.00000410