##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553443_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	720886
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.249831457401033	31.0	31.0	34.0	28.0	34.0
2	31.466206862111346	31.0	31.0	34.0	28.0	34.0
3	31.660920034513087	31.0	31.0	34.0	30.0	34.0
4	35.45398162816312	37.0	35.0	37.0	33.0	37.0
5	35.18571868506255	37.0	35.0	37.0	32.0	37.0
6	32.38543680970361	36.0	35.0	37.0	25.0	37.0
7	34.01532835982388	37.0	35.0	37.0	26.0	37.0
8	35.13016482495152	37.0	35.0	37.0	32.0	37.0
9	36.93653920314724	39.0	37.0	39.0	33.0	39.0
10	36.26297778012057	38.0	35.0	39.0	32.0	39.0
11	36.4306575519569	38.0	35.0	39.0	32.0	39.0
12	36.525438141398226	38.0	35.0	39.0	32.0	39.0
13	36.52448930898922	38.0	35.0	39.0	32.0	39.0
14	37.55119672181177	39.0	36.0	40.0	32.0	41.0
15	37.56921621449161	39.0	36.0	40.0	32.0	41.0
16	37.4952613866825	39.0	36.0	40.0	32.0	41.0
17	37.490098295708336	39.0	36.0	40.0	32.0	41.0
18	37.34824785056167	39.0	36.0	40.0	32.0	41.0
19	37.267093548771925	39.0	35.0	40.0	32.0	41.0
20	37.16834145759524	39.0	35.0	40.0	32.0	41.0
21	37.094848561353665	39.0	35.0	40.0	31.0	41.0
22	37.0513104707263	39.0	35.0	40.0	31.0	41.0
23	36.87795989934608	39.0	35.0	40.0	31.0	41.0
24	36.75312046564922	39.0	35.0	40.0	31.0	41.0
25	36.81830969113008	39.0	35.0	40.0	31.0	41.0
26	36.697389046256966	39.0	35.0	40.0	31.0	41.0
27	36.6532419827823	39.0	35.0	40.0	31.0	41.0
28	36.63579262185699	39.0	35.0	40.0	31.0	41.0
29	36.4163251887261	38.0	35.0	40.0	30.0	41.0
30	36.417480711235896	38.0	35.0	40.0	30.0	41.0
31	36.02515099474813	38.0	35.0	40.0	30.0	41.0
32	36.10703911575478	38.0	35.0	40.0	30.0	41.0
33	36.14367042777915	38.0	35.0	40.0	30.0	41.0
34	36.123226141165176	38.0	35.0	40.0	30.0	41.0
35	36.1175719878039	38.0	35.0	40.0	30.0	41.0
36	36.04868037387326	38.0	35.0	40.0	30.0	41.0
37	35.92936608562241	38.0	35.0	40.0	30.0	41.0
38	35.855135208618286	38.0	35.0	40.0	29.0	41.0
39	35.736821078506175	38.0	35.0	40.0	29.0	41.0
40	35.6162458419223	38.0	35.0	40.0	28.0	41.0
41	35.66936242346224	38.0	35.0	40.0	29.0	41.0
42	35.55051006677893	38.0	35.0	40.0	28.0	41.0
43	35.50383139636503	38.0	34.0	40.0	28.0	41.0
44	35.30746192879318	38.0	34.0	40.0	27.0	41.0
45	35.11893142605072	38.0	34.0	40.0	26.0	41.0
46	34.8214433350072	37.0	33.0	40.0	25.0	41.0
47	34.85960470865019	37.0	33.0	40.0	25.0	41.0
48	34.85926901063414	37.0	33.0	40.0	25.0	41.0
49	34.73280379976862	37.0	33.0	40.0	25.0	41.0
50	34.608035112347864	37.0	33.0	40.0	24.0	41.0
51	33.43697893980463	36.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	5.0
13	9.0
14	13.0
15	40.0
16	96.0
17	213.0
18	424.0
19	815.0
20	1418.0
21	2120.0
22	2927.0
23	3823.0
24	4586.0
25	5589.0
26	6365.0
27	7971.0
28	9988.0
29	13202.0
30	17001.0
31	22466.0
32	29365.0
33	38767.0
34	53596.0
35	75644.0
36	83205.0
37	103992.0
38	129407.0
39	107817.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.746375987326704	33.85139952780329	20.713816054133385	26.688408430736622
2	20.472862560793246	34.5157209322972	20.945752865224183	24.06566364168537
3	19.268095094092548	34.361188870362305	22.50938428544874	23.861331750096408
4	18.507087112247984	35.01302563789559	20.483821297680908	25.996065952175517
5	16.271504787164684	38.94416038042076	18.387512033802846	26.39682279861171
6	17.03084815074783	43.250111668141706	18.678542793173953	21.04049738793651
7	78.20501438507614	10.796297888986608	6.085844363741285	4.912843362195964
8	79.91679683056682	9.230030823181474	4.847090940869985	6.00608140538171
9	72.85978642947705	13.759457112497678	8.99837144846758	4.382385009557683
10	25.51499127462595	46.88480564194616	13.065311297486703	14.53489178594119
11	16.70375066237935	31.82611397641236	34.021468026844744	17.448667334363545
12	20.465787933182224	27.2380098933812	33.692983356591746	18.603218816844826
13	21.28145088127665	28.441528896385837	29.54489336732854	20.732126855008975
14	16.548247573125295	31.33796467125177	31.56879173683495	20.54499601878799
15	14.76530269695902	34.78344703600847	28.969906476197345	21.481343790835165
16	16.623155394889068	33.70130644789884	28.403797549127045	21.27174060808505
17	15.226401955371585	33.27488673659913	29.72730778514217	21.771403522887116
18	15.73424924329228	32.793257186295754	27.89997308867144	23.572520481740526
19	16.715264272020818	36.50632693657527	28.998759859395246	17.77964893200867
20	18.01311164317243	34.23814583720588	28.206540285149106	19.54220223447258
21	17.49166997278349	35.14161739859007	27.011066937074656	20.355645691551786
22	17.002549640303737	32.485441526122024	28.1137378170751	22.39827101649914
23	17.911431211037527	34.36715375246572	25.711416229473176	22.009998807023578
24	13.939097166542282	35.914000271887645	27.451358467219507	22.695544094350563
25	17.38236004028376	33.647206354402776	25.921713003165546	23.048720602147913
26	18.22007918034197	34.59784209986045	24.626501277594514	22.555577442203067
27	14.851862846552713	33.90744167593767	26.42900541833244	24.81169005917718
28	17.745246821272712	32.272647825037524	27.09110733181113	22.890998021878634
29	18.155713940900505	31.91031591680238	26.83350765585682	23.100462486440296
30	17.55839342142863	31.250017339773557	28.86018038913226	22.33140884966555
31	19.313594659904616	32.86885859900178	23.315198242163117	24.50234849893048
32	18.121034393787642	30.355701178827164	28.14938839150712	23.373876035878073
33	18.061801727318883	30.40369767203136	27.778594673776436	23.755905926873318
34	16.015153574906435	29.239019761793127	27.424863293225282	27.32096337007516
35	16.4037032207589	28.494241807997383	30.576817971218752	24.52523700002497
36	17.77909405925486	29.869771364681796	29.64560277214428	22.705531803919065
37	17.51663924670475	31.429379957441263	28.9754552037354	22.078525592118588
38	18.95029172435031	29.82496538981198	32.25489189691574	18.969850988921966
39	17.607915814705795	29.37940256850598	30.647009374575173	22.365672242213055
40	18.423994917365576	29.362895104080255	28.735334019525972	23.477775959028197
41	16.363058791542628	27.910099516428392	31.63856698562602	24.088274706402952
42	19.245622747563417	29.40159747865821	26.90855419580905	24.444225577969334
43	16.29037046079408	29.441687035120673	29.3142050199338	24.953737484151446
44	19.558432262521396	28.290603507350674	27.568436618272514	24.58252761185541
45	20.50546133507933	29.790563279076025	27.989307602034163	21.71466778381048
46	16.792391584799816	29.01762553302464	31.867313278382436	22.32266960379311
47	17.800734096653283	28.638924878552224	28.967132112428317	24.593208912366173
48	17.92405456618661	27.26339532186781	32.275977061560354	22.53657305038522
49	18.762189860810167	27.512394470138133	30.222670436102238	23.502745232949454
50	18.15737855916192	26.491012448570235	28.15133044614544	27.20027854612241
51	17.80017922389948	25.63942148966688	32.226315950094744	24.334083336338892
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20634.0
1	16213.0
2	11792.0
3	7343.0
4	2894.0
5	2366.0
6	1838.0
7	1997.5
8	2157.0
9	2564.0
10	2971.0
11	3846.0
12	4721.0
13	4856.0
14	4991.0
15	5271.0
16	5551.0
17	5260.0
18	4969.0
19	5184.5
20	5400.0
21	4970.0
22	4540.0
23	4482.5
24	4425.0
25	5843.5
26	7843.5
27	8425.0
28	9995.5
29	11566.0
30	13863.5
31	16161.0
32	16651.0
33	17141.0
34	20118.5
35	23096.0
36	24793.5
37	26491.0
38	27436.0
39	28381.0
40	29712.0
41	31043.0
42	35335.0
43	39627.0
44	50232.0
45	60837.0
46	64334.0
47	67831.0
48	72844.5
49	77858.0
50	69892.5
51	61927.0
52	53509.5
53	45092.0
54	39760.0
55	34428.0
56	28312.5
57	22197.0
58	20626.0
59	19055.0
60	16155.0
61	13255.0
62	11580.0
63	9905.0
64	8516.0
65	7127.0
66	6253.0
67	5379.0
68	4246.5
69	3114.0
70	2817.0
71	2520.0
72	2114.0
73	1708.0
74	1416.5
75	869.5
76	614.0
77	555.0
78	496.0
79	355.0
80	214.0
81	131.5
82	49.0
83	46.5
84	44.0
85	32.0
86	20.0
87	13.0
88	6.0
89	4.5
90	3.0
91	4.0
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	720886.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.83022718602607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.06652232887494	22.09421187949212
2	10.670303987177235	6.365951841629134
3	4.316804412566432	3.8631376903348946
4	2.3329907151487217	2.7837457222310324
5	1.44525627756774	2.155616155093802
6	1.0265189442816036	1.8372775991207926
7	0.7218282306485373	1.5072610076673165
8	0.5886320256333425	1.4047221642890664
9	0.48071973873097956	1.2905981117236995
>10	3.4515032814884723	21.282928132848294
>50	0.5524955133837972	11.758828001792656
>100	0.3356281156046485	16.151865427881873
>500	0.006102329374629973	1.216826418679776
>1k	0.0037552796151569066	2.8400084492132587
>5k	4.694099518946133E-4	0.9385946472279101
>10k+	4.694099518946133E-4	2.508426750774397
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17914	2.4849976279189776	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT	6703	0.9298280171899579	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4582	0.6356067394844677	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3660	0.5077085697322461	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGT	2973	0.4124091742661114	No Hit
AATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	2597	0.36025113540837245	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2077	0.2881176774136271	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCC	1747	0.2423406752246541	No Hit
AAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	1377	0.19101494549762377	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT	1269	0.17603338114486894	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	888	0.12318175134487283	No Hit
AATGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT	868	0.12040738757584417	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	774	0.10736787786140944	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	759	0.10528710503463794	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT	727	0.10084812300419208	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7743637690286676E-4	0.0	0.0	1.0758982696293173	0.0
2	2.7743637690286676E-4	0.0	0.0	1.4211678406849348	0.0
3	4.161545653543001E-4	0.0	0.0	1.8642337345988131	0.0
4	4.161545653543001E-4	0.0	0.0	3.8907677496858035	0.0
5	4.161545653543001E-4	0.0	0.0	4.188179545725676	0.0
6	4.161545653543001E-4	0.0	0.0	5.882483499471483	0.0
7	4.161545653543001E-4	0.0	0.0	7.349567060533843	0.0
8	5.548727538057335E-4	0.0	0.0	8.378301146089672	0.0
9	5.548727538057335E-4	0.0	0.0	10.203832506110537	0.0
10	5.548727538057335E-4	0.0	0.0	12.488243633528741	0.0
11	5.548727538057335E-4	0.0	0.0	14.601337798209425	0.0
12	5.548727538057335E-4	0.0	0.0	15.714412542343727	0.0
13	5.548727538057335E-4	0.0	0.0	16.178563600902223	0.0
14	5.548727538057335E-4	0.0	0.0	16.63120104981925	0.0
15	5.548727538057335E-4	0.0	0.0	17.009346831537858	0.0
16	5.548727538057335E-4	0.0	0.0	17.720693701916808	0.0
17	5.548727538057335E-4	0.0	0.0	18.73056211384324	0.0
18	5.548727538057335E-4	0.0	0.0	20.054765940800625	0.0
19	5.548727538057335E-4	0.0	0.0	20.610193567360167	0.0
20	5.548727538057335E-4	0.0	0.0	21.134132165141228	0.0
21	5.548727538057335E-4	0.0	0.0	21.743382448819926	0.0
22	5.548727538057335E-4	0.0	0.0	22.458197273910162	0.0
23	5.548727538057335E-4	0.0	0.0	23.1247381694193	0.0
24	5.548727538057335E-4	0.0	0.0	23.591941028123724	0.0
25	5.548727538057335E-4	0.0	0.0	23.988536328906374	0.0
26	5.548727538057335E-4	0.0	0.0	24.38194111135464	0.0
27	5.548727538057335E-4	0.0	0.0	24.80225722236248	0.0
28	5.548727538057335E-4	0.0	0.0	25.245739270841714	0.0
29	5.548727538057335E-4	0.0	0.0	25.696989537874227	0.0
30	5.548727538057335E-4	0.0	0.0	26.272670019947675	0.0
31	5.548727538057335E-4	0.0	0.0	26.69506690378229	0.0
32	5.548727538057335E-4	0.0	0.0	27.089303995361263	0.0
33	5.548727538057335E-4	0.0	0.0	27.519330379560706	0.0
34	5.548727538057335E-4	0.0	0.0	27.918145171358578	0.0
35	5.548727538057335E-4	0.0	0.0	28.358159265126524	0.0
36	5.548727538057335E-4	0.0	0.0	28.778475376134367	0.0
37	5.548727538057335E-4	0.0	0.0	29.21307946055271	0.0
38	5.548727538057335E-4	0.0	0.0	29.608565015827747	0.0
39	5.548727538057335E-4	0.0	0.0	30.01917085364399	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAC	20	7.031533E-4	45.000004	4
CGTATAA	20	7.031533E-4	45.000004	26
CAACGTA	20	7.031533E-4	45.000004	44
CTCCGCA	20	7.031533E-4	45.000004	44
CGCATTC	20	7.031533E-4	45.000004	14
CTCCCGG	75	0.0	45.000004	2
AGGCGTC	20	7.031533E-4	45.000004	32
GCGTTTC	20	7.031533E-4	45.000004	29
CCGCTTC	20	7.031533E-4	45.000004	39
CCCACCG	20	7.031533E-4	45.000004	1
CGTTACT	20	7.031533E-4	45.000004	29
GTTACGT	20	7.031533E-4	45.000004	31
TACGCGT	20	7.031533E-4	45.000004	22
CTAGTCG	20	7.031533E-4	45.000004	37
TACGAAG	55	1.8189894E-12	45.000004	1
ACGTGTT	20	7.031533E-4	45.000004	45
TGTTCCG	20	7.031533E-4	45.000004	1
GACGGTG	20	7.031533E-4	45.000004	26
GTCCGCC	20	7.031533E-4	45.000004	21
AACGACC	20	7.031533E-4	45.000004	10
>>END_MODULE