FastQCFastQC Report
Sat 18 Jun 2016
SRR3553442_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553442_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376067
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA158704.219992714064249No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35210.9362693349855212No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25540.6791343031959731No Hit
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT18200.4839563162947028TruSeq Adapter, Index 3 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13380.355787665495776No Hit
ACCTAGGGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA11730.31191250495257494No Hit
ATGCAGGGGTTGCTTGTTTTGTTTTAATCTGAAGGTTCTGGTAACCTGTTT5420.14412325463281808No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC5190.13800732316315975RNA PCR Primer, Index 3 (95% over 23bp)
CGCGACGGGATACCACCACACCTAGAGTTAGAATTACATTTTTTTTTTAAT4490.11939361869028656No Hit
GGTTACGGGGAACACACACTCCTCTACACAAACATACACACACTCCTCTAC3830.10184355447300615No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG302.1615924E-645.0000041
TATCACC207.027226E-445.00000418
CAGAACG351.2090095E-745.0000041
GTACCGG207.027226E-445.0000042
CCCCTAT351.2090095E-745.00000439
AGTCCAT351.2090095E-745.00000437
TCGCAGG600.045.0000042
CTCGTAG351.2090095E-745.0000041
CTGTAGG406.7939254E-945.0000042
CTCAACG302.1615924E-645.0000041
CAACGTG302.1615924E-645.0000041
CGAAATC207.027226E-445.00000424
CGAAAGT207.027226E-445.00000442
TCCGATG302.1615924E-645.0000041
CTATCCG351.2090095E-745.0000041
GAGTCGA207.027226E-445.00000429
GACCGTT351.2090095E-745.00000434
ATCAGCG351.2090095E-745.0000041
TAAACGG700.045.0000042
CGCGCAT351.2090095E-745.00000438