Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553442_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 376067 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 40 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 15870 | 4.219992714064249 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3521 | 0.9362693349855212 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2554 | 0.6791343031959731 | No Hit |
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT | 1820 | 0.4839563162947028 | TruSeq Adapter, Index 3 (95% over 24bp) |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1338 | 0.355787665495776 | No Hit |
ACCTAGGGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA | 1173 | 0.31191250495257494 | No Hit |
ATGCAGGGGTTGCTTGTTTTGTTTTAATCTGAAGGTTCTGGTAACCTGTTT | 542 | 0.14412325463281808 | No Hit |
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC | 519 | 0.13800732316315975 | RNA PCR Primer, Index 3 (95% over 23bp) |
CGCGACGGGATACCACCACACCTAGAGTTAGAATTACATTTTTTTTTTAAT | 449 | 0.11939361869028656 | No Hit |
GGTTACGGGGAACACACACTCCTCTACACAAACATACACACACTCCTCTAC | 383 | 0.10184355447300615 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCACGCG | 30 | 2.1615924E-6 | 45.000004 | 1 |
TATCACC | 20 | 7.027226E-4 | 45.000004 | 18 |
CAGAACG | 35 | 1.2090095E-7 | 45.000004 | 1 |
GTACCGG | 20 | 7.027226E-4 | 45.000004 | 2 |
CCCCTAT | 35 | 1.2090095E-7 | 45.000004 | 39 |
AGTCCAT | 35 | 1.2090095E-7 | 45.000004 | 37 |
TCGCAGG | 60 | 0.0 | 45.000004 | 2 |
CTCGTAG | 35 | 1.2090095E-7 | 45.000004 | 1 |
CTGTAGG | 40 | 6.7939254E-9 | 45.000004 | 2 |
CTCAACG | 30 | 2.1615924E-6 | 45.000004 | 1 |
CAACGTG | 30 | 2.1615924E-6 | 45.000004 | 1 |
CGAAATC | 20 | 7.027226E-4 | 45.000004 | 24 |
CGAAAGT | 20 | 7.027226E-4 | 45.000004 | 42 |
TCCGATG | 30 | 2.1615924E-6 | 45.000004 | 1 |
CTATCCG | 35 | 1.2090095E-7 | 45.000004 | 1 |
GAGTCGA | 20 | 7.027226E-4 | 45.000004 | 29 |
GACCGTT | 35 | 1.2090095E-7 | 45.000004 | 34 |
ATCAGCG | 35 | 1.2090095E-7 | 45.000004 | 1 |
TAAACGG | 70 | 0.0 | 45.000004 | 2 |
CGCGCAT | 35 | 1.2090095E-7 | 45.000004 | 38 |