##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553442_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	376067
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57111631703925	31.0	31.0	34.0	30.0	34.0
2	31.767908378028384	31.0	31.0	34.0	30.0	34.0
3	31.96526682745362	33.0	31.0	34.0	30.0	34.0
4	35.71016334855225	37.0	35.0	37.0	33.0	37.0
5	35.434457157900056	37.0	35.0	37.0	33.0	37.0
6	34.03974025904959	37.0	35.0	37.0	32.0	37.0
7	34.914839642935966	37.0	35.0	37.0	32.0	37.0
8	35.61448093025977	37.0	35.0	37.0	32.0	37.0
9	37.27075228616177	39.0	37.0	39.0	34.0	39.0
10	36.649804423148005	38.0	35.0	39.0	32.0	39.0
11	36.703893189245534	39.0	37.0	39.0	32.0	39.0
12	36.78308120627442	39.0	37.0	39.0	32.0	39.0
13	36.775034767740856	39.0	37.0	39.0	32.0	39.0
14	37.93429894141204	40.0	37.0	41.0	33.0	41.0
15	37.9090162125366	40.0	37.0	41.0	33.0	41.0
16	37.821627529137096	39.0	37.0	41.0	33.0	41.0
17	37.77210709793734	39.0	37.0	41.0	33.0	41.0
18	37.56810089691464	39.0	36.0	40.0	33.0	41.0
19	37.43898028808697	39.0	35.0	40.0	32.0	41.0
20	37.32835904240468	39.0	35.0	40.0	32.0	41.0
21	37.29353811953721	39.0	35.0	40.0	32.0	41.0
22	37.246487992831064	39.0	35.0	40.0	32.0	41.0
23	37.02245344579556	39.0	35.0	40.0	32.0	41.0
24	36.81317158910513	39.0	35.0	40.0	31.0	41.0
25	36.88428391749343	39.0	35.0	40.0	31.0	41.0
26	36.80625792744378	39.0	35.0	40.0	31.0	41.0
27	36.786474218689754	39.0	35.0	40.0	31.0	41.0
28	36.68188381325668	39.0	35.0	40.0	31.0	41.0
29	36.55044978687308	38.0	35.0	40.0	31.0	41.0
30	36.50645230770049	38.0	35.0	40.0	31.0	41.0
31	36.433667405010276	38.0	35.0	40.0	30.0	41.0
32	36.30650655335352	38.0	35.0	40.0	30.0	41.0
33	36.24121499626396	38.0	35.0	40.0	30.0	41.0
34	36.1669463154172	38.0	35.0	40.0	30.0	41.0
35	36.03125240980995	38.0	35.0	40.0	30.0	41.0
36	35.93821047845198	38.0	35.0	40.0	30.0	41.0
37	35.88772213461963	38.0	35.0	40.0	30.0	41.0
38	35.808441049068385	38.0	35.0	40.0	29.0	41.0
39	35.77012074976002	38.0	35.0	40.0	29.0	41.0
40	35.639463180763	38.0	35.0	40.0	29.0	41.0
41	35.57033454145139	38.0	35.0	40.0	28.0	41.0
42	35.43832880843041	38.0	34.0	40.0	27.0	41.0
43	35.34044997301013	38.0	34.0	40.0	27.0	41.0
44	35.173562157806984	38.0	34.0	40.0	26.0	41.0
45	35.05533056609593	38.0	34.0	40.0	26.0	41.0
46	34.88556826310205	37.0	34.0	40.0	25.0	41.0
47	34.806353655066786	37.0	34.0	40.0	25.0	41.0
48	34.66993381498509	37.0	33.0	40.0	24.0	41.0
49	34.490295612218034	37.0	33.0	40.0	24.0	41.0
50	34.37217304363318	37.0	33.0	40.0	24.0	41.0
51	33.33166962270021	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	3.0
11	1.0
12	3.0
13	4.0
14	7.0
15	10.0
16	49.0
17	110.0
18	235.0
19	442.0
20	760.0
21	1148.0
22	1550.0
23	2035.0
24	2590.0
25	3034.0
26	3403.0
27	4082.0
28	4877.0
29	6100.0
30	8120.0
31	10526.0
32	13713.0
33	18088.0
34	26395.0
35	40637.0
36	40297.0
37	51519.0
38	70093.0
39	66225.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.272629611212896	35.654019097660786	20.238680873355015	24.834670417771303
2	20.893883270800153	35.33173610021618	20.522672821598277	23.251707807385387
3	19.321025242842367	36.85460303616111	19.729197190926083	24.09517453007044
4	18.352846700189062	38.30567425485352	18.406294623032597	24.935184421924816
5	16.14100678868393	41.727936777223206	15.87988310593591	26.251173328156952
6	17.024360020953715	45.0028851241933	16.260666317438115	21.712088537414875
7	80.06206340891384	12.960722424461599	3.9011665474503214	3.076047619174243
8	81.65965107281416	12.261378956409363	2.9492085186948067	3.1297614520816768
9	73.89507720698705	17.37722267574661	5.787798450807967	2.9399016664583706
10	24.8349363278352	51.8128418606259	11.299582255289616	12.052639556249286
11	16.74143171296604	36.9588397812092	30.872424328643568	15.427304177181195
12	22.702869435499526	30.561309553882687	31.072920516822798	15.662900493794988
13	21.650929222718293	33.338740171299264	26.894675682790563	18.11565492319188
14	16.392291799067717	36.926664663477524	26.452467246527878	20.228576290926885
15	15.0739097022605	39.64187232594192	27.170956239180782	18.113261732616795
16	17.89787458085926	37.256924962839065	25.66617118758094	19.179029268720733
17	17.121417194276553	35.810108305169024	26.406730715537392	20.66174378501703
18	17.428809228142857	37.30585241459634	25.245767376557897	20.019570980702905
19	17.16981282590602	39.42568744399269	25.80630579125528	17.598193938846006
20	19.64623325098985	37.032496868909	25.60953234397062	17.71173753613053
21	19.356391281340827	37.769333655970875	24.7612792401354	18.112995822552897
22	17.981104430859396	36.141964064913964	25.70791906761295	20.16901243661369
23	17.810390169836758	38.76277365469451	23.56814078342423	19.858695392044503
24	15.924024176543009	39.56688568792263	25.5390661770376	18.970023958496757
25	16.135954497469864	39.89289142626181	25.107228233266945	18.86392584300138
26	18.046784216642248	39.57858573073415	23.700563995245528	18.674066057378074
27	15.490058952261165	38.37161995070028	25.4071747853441	20.73114631169446
28	17.113173982295706	38.0586438054921	27.329704547328003	17.49847766488418
29	19.190995221596154	36.88970316459567	23.031268364413787	20.88803324939439
30	17.424820577184384	38.43623609622753	25.937133542693193	18.20180978389489
31	20.059989310415432	36.99633310021884	22.969577229589408	19.97410035977632
32	19.543857876389048	37.4584847912739	23.631959198759795	19.36569813357726
33	17.74125355322323	37.06866063759915	25.073191745087975	20.116894064089642
34	18.08188434507681	36.29087370069695	25.599427761542493	20.027814192683753
35	19.95947530626192	36.011668133603855	25.002193758027158	19.026662802107072
36	20.209164856262316	36.609965777374775	25.633730159785355	17.54713920657755
37	19.236997662650538	37.378445862040536	24.179468020326166	19.205088454982754
38	18.02737278197768	37.07052200804644	26.00813152975401	18.893973680221876
39	17.96993620817567	37.015478624819515	24.84450909013553	20.170076076869282
40	20.06318023118221	35.818617427213766	26.037913456910605	18.080288884693417
41	18.769793680381422	33.36905391858366	27.32598180643343	20.535170594601492
42	19.470998518880943	35.04854188216461	25.402122494130037	20.078337104824406
43	18.34433757814432	35.677685093347726	24.805952130870295	21.172025197637655
44	19.465680317602978	34.754179441429315	24.981984593170896	20.7981556477968
45	20.329356205144297	35.03763956954479	24.69134489333018	19.941659331980738
46	17.558307429261273	36.77562774718335	27.01858977256712	18.647475050988255
47	18.056091068878686	36.185041495265466	25.841671829753743	19.917195606102105
48	18.779898262809553	34.11652710820147	27.672462619692766	19.431112009296218
49	19.5659284116926	34.110677086795704	25.941388103715564	20.382006397796136
50	17.5987257589738	33.45733605979785	26.6106837345473	22.333254446681046
51	18.24275993373521	33.15233721650663	27.869501977041327	20.73540087271683
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17727.0
1	13044.0
2	8361.0
3	5261.5
4	2162.0
5	1770.0
6	1378.0
7	1447.5
8	1517.0
9	1774.5
10	2032.0
11	2411.0
12	2790.0
13	2945.5
14	3101.0
15	3386.5
16	3672.0
17	3401.0
18	3130.0
19	3212.0
20	3294.0
21	3100.5
22	2907.0
23	3047.0
24	3187.0
25	3617.5
26	4185.0
27	4322.0
28	6129.5
29	7937.0
30	8010.0
31	8083.0
32	9354.0
33	10625.0
34	12268.5
35	13912.0
36	14851.5
37	15791.0
38	15702.5
39	15614.0
40	17413.5
41	19213.0
42	19887.0
43	20561.0
44	22488.0
45	24415.0
46	26967.5
47	29520.0
48	32274.0
49	35028.0
50	32432.5
51	29837.0
52	26492.5
53	23148.0
54	19368.0
55	15588.0
56	13497.5
57	11407.0
58	9819.0
59	8231.0
60	7517.0
61	6803.0
62	6197.0
63	5591.0
64	4641.0
65	3691.0
66	3151.0
67	2611.0
68	2145.0
69	1679.0
70	1479.0
71	1279.0
72	962.5
73	646.0
74	627.5
75	493.5
76	378.0
77	273.5
78	169.0
79	103.5
80	38.0
81	26.5
82	15.0
83	11.0
84	7.0
85	6.0
86	5.0
87	3.5
88	2.0
89	2.5
90	3.0
91	3.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	376067.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.760343714554324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.91048499171075	20.240201237647447
2	10.962364779245418	6.086380283923544
3	4.146631622337752	3.4533575728120804
4	2.1439631968399686	2.3806862102252886
5	1.4238640268274116	1.9763477393759172
6	1.0159584620675055	1.6922013664022375
7	0.8444598435450094	1.6409746856963763
8	0.6201332063870978	1.377208876649159
9	0.524421137393026	1.3102299922687124
>10	4.396762466975843	25.740454489593894
>50	0.6889258188058544	13.450475861441141
>100	0.31405446153957184	13.085416030558372
>500	0.0019939965812036304	0.2936531315895521
>1k	0.004984991453009077	2.880070204826464
>5k	0.0	0.0
>10k+	9.969982906018152E-4	4.392342316989812
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15870	4.219992714064249	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3521	0.9362693349855212	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2554	0.6791343031959731	No Hit
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	1820	0.4839563162947028	TruSeq Adapter, Index 3 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1338	0.355787665495776	No Hit
ACCTAGGGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1173	0.31191250495257494	No Hit
ATGCAGGGGTTGCTTGTTTTGTTTTAATCTGAAGGTTCTGGTAACCTGTTT	542	0.14412325463281808	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	519	0.13800732316315975	RNA PCR Primer, Index 3 (95% over 23bp)
CGCGACGGGATACCACCACACCTAGAGTTAGAATTACATTTTTTTTTTAAT	449	0.11939361869028656	No Hit
GGTTACGGGGAACACACACTCCTCTACACAAACATACACACACTCCTCTAC	383	0.10184355447300615	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5496361020775553	0.0
2	0.0	0.0	0.0	0.827246208787264	0.0
3	0.0	0.0	0.0	1.0458242813115748	0.0
4	0.0	0.0	0.0	2.0608029952109597	0.0
5	0.0	0.0	0.0	2.223008134188855	0.0
6	0.0	0.0	0.0	3.1630002100689505	0.0
7	0.0	0.0	0.0	3.892125605277783	0.0
8	0.0	0.0	0.0	4.5236620070359805	0.0
9	0.0	0.0	0.0	5.46897228419404	0.0
10	0.0	0.0	0.0	6.955675451448811	0.0
11	0.0	0.0	0.0	8.114511509917115	0.0
12	0.0	0.0	0.0	8.713340973815836	0.0
13	0.0	0.0	0.0	8.945746369662853	0.0
14	0.0	0.0	0.0	9.208199602730364	0.0
15	0.0	0.0	0.0	9.454964142027883	0.0
16	0.0	0.0	0.0	9.987316089952056	0.0
17	0.0	0.0	0.0	10.728939258164104	0.0
18	0.0	0.0	0.0	11.613622040753377	0.0
19	0.0	0.0	0.0	12.020730348581504	0.0
20	0.0	0.0	0.0	12.45682285337453	0.0
21	0.0	0.0	0.0	12.899563109765015	0.0
22	0.0	0.0	0.0	13.412237712960723	0.0
23	0.0	0.0	0.0	13.894864478935935	0.0
24	0.0	0.0	0.0	14.256236255773572	0.0
25	0.0	0.0	0.0	14.570010131173435	0.0
26	0.0	0.0	0.0	14.876870344911945	0.0
27	0.0	0.0	0.0	15.232923920471618	0.0
28	0.0	0.0	0.0	15.573820622389096	0.0
29	0.0	0.0	0.0	15.926151457054194	0.0
30	0.0	0.0	0.0	16.389898608492636	0.0
31	0.0	0.0	0.0	16.72707256951554	0.0
32	0.0	0.0	0.0	17.060789699707765	0.0
33	0.0	0.0	0.0	17.417641005459135	0.0
34	0.0	0.0	0.0	17.754814966482037	0.0
35	0.0	0.0	0.0	18.10794353133883	0.0
36	0.0	0.0	0.0	18.44830841312851	0.0
37	0.0	0.0	0.0	18.81154156041344	0.0
38	0.0	0.0	0.0	19.166797405781416	0.0
39	0.0	0.0	0.0	19.51274639891296	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	30	2.1615924E-6	45.000004	1
TATCACC	20	7.027226E-4	45.000004	18
CAGAACG	35	1.2090095E-7	45.000004	1
GTACCGG	20	7.027226E-4	45.000004	2
CCCCTAT	35	1.2090095E-7	45.000004	39
AGTCCAT	35	1.2090095E-7	45.000004	37
TCGCAGG	60	0.0	45.000004	2
CTCGTAG	35	1.2090095E-7	45.000004	1
CTGTAGG	40	6.7939254E-9	45.000004	2
CTCAACG	30	2.1615924E-6	45.000004	1
CAACGTG	30	2.1615924E-6	45.000004	1
CGAAATC	20	7.027226E-4	45.000004	24
CGAAAGT	20	7.027226E-4	45.000004	42
TCCGATG	30	2.1615924E-6	45.000004	1
CTATCCG	35	1.2090095E-7	45.000004	1
GAGTCGA	20	7.027226E-4	45.000004	29
GACCGTT	35	1.2090095E-7	45.000004	34
ATCAGCG	35	1.2090095E-7	45.000004	1
TAAACGG	70	0.0	45.000004	2
CGCGCAT	35	1.2090095E-7	45.000004	38
>>END_MODULE