FastQCFastQC Report
Sat 18 Jun 2016
SRR3553439_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553439_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520786
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA154252.961869174670594No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36820.7070082529100244No Hit
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT24680.473899067947295TruSeq Adapter, Index 14 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24280.46621836992545884No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16230.3116443222360048No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGT8450.16225474571129025No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCC7570.14535721006325056No Hit
AAGCGAGGGAAGGAAATTGAAAAAGGAACCTAATTAATTCTTACAAATTGC7320.1405567737996029No Hit
AATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT6520.12519537775593045No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG5910.11348231327263023No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGT207.02973E-445.019
CGGACAG253.887857E-545.01
TCACGAG207.02973E-445.01
CCTTCCG207.02973E-445.019
TTAGGTC207.02973E-445.019
CGGCGGA207.02973E-445.018
AATCCGA207.02973E-445.017
TCGTTCA253.887857E-545.016
TCGCACA207.02973E-445.015
CGAACAG453.8380676E-1045.01
GGTGTTA207.02973E-445.09
GCGACGC453.8380676E-1045.015
TCCGCGG207.02973E-445.02
GTCTGCG351.21021E-745.01
CAACGAA207.02973E-445.032
CATGCGG207.02973E-445.02
CGACGAA207.02973E-445.017
TAGACGG700.045.02
TAGCGAG351.21021E-745.01
GTCTCGC351.21021E-745.025