Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553439_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 520786 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 42 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 15425 | 2.961869174670594 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3682 | 0.7070082529100244 | No Hit |
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT | 2468 | 0.473899067947295 | TruSeq Adapter, Index 14 (95% over 23bp) |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2428 | 0.46621836992545884 | No Hit |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1623 | 0.3116443222360048 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGT | 845 | 0.16225474571129025 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCC | 757 | 0.14535721006325056 | No Hit |
AAGCGAGGGAAGGAAATTGAAAAAGGAACCTAATTAATTCTTACAAATTGC | 732 | 0.1405567737996029 | No Hit |
AATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT | 652 | 0.12519537775593045 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 591 | 0.11348231327263023 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGATCGT | 20 | 7.02973E-4 | 45.0 | 19 |
CGGACAG | 25 | 3.887857E-5 | 45.0 | 1 |
TCACGAG | 20 | 7.02973E-4 | 45.0 | 1 |
CCTTCCG | 20 | 7.02973E-4 | 45.0 | 19 |
TTAGGTC | 20 | 7.02973E-4 | 45.0 | 19 |
CGGCGGA | 20 | 7.02973E-4 | 45.0 | 18 |
AATCCGA | 20 | 7.02973E-4 | 45.0 | 17 |
TCGTTCA | 25 | 3.887857E-5 | 45.0 | 16 |
TCGCACA | 20 | 7.02973E-4 | 45.0 | 15 |
CGAACAG | 45 | 3.8380676E-10 | 45.0 | 1 |
GGTGTTA | 20 | 7.02973E-4 | 45.0 | 9 |
GCGACGC | 45 | 3.8380676E-10 | 45.0 | 15 |
TCCGCGG | 20 | 7.02973E-4 | 45.0 | 2 |
GTCTGCG | 35 | 1.21021E-7 | 45.0 | 1 |
CAACGAA | 20 | 7.02973E-4 | 45.0 | 32 |
CATGCGG | 20 | 7.02973E-4 | 45.0 | 2 |
CGACGAA | 20 | 7.02973E-4 | 45.0 | 17 |
TAGACGG | 70 | 0.0 | 45.0 | 2 |
TAGCGAG | 35 | 1.21021E-7 | 45.0 | 1 |
GTCTCGC | 35 | 1.21021E-7 | 45.0 | 25 |