##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553439_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	520786
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.492576605361894	31.0	31.0	34.0	30.0	34.0
2	31.71010933473634	31.0	31.0	34.0	30.0	34.0
3	31.87614874439789	33.0	31.0	34.0	30.0	34.0
4	35.635861947133755	37.0	35.0	37.0	33.0	37.0
5	35.38174029255779	37.0	35.0	37.0	33.0	37.0
6	32.34915685137465	37.0	35.0	37.0	25.0	37.0
7	34.041398962337695	37.0	35.0	37.0	25.0	37.0
8	35.249787820717145	37.0	35.0	37.0	32.0	37.0
9	37.11708072029586	39.0	37.0	39.0	34.0	39.0
10	36.53403125276025	38.0	35.0	39.0	32.0	39.0
11	36.63934322351215	39.0	35.0	39.0	32.0	39.0
12	36.683192328518814	39.0	35.0	39.0	32.0	39.0
13	36.69541231907156	39.0	35.0	39.0	32.0	39.0
14	37.79482167339368	39.0	37.0	41.0	33.0	41.0
15	37.84086553786008	39.0	37.0	41.0	33.0	41.0
16	37.7755200792648	39.0	37.0	41.0	33.0	41.0
17	37.76406815851425	39.0	37.0	41.0	33.0	41.0
18	37.580693413417414	39.0	36.0	40.0	33.0	41.0
19	37.53486076814661	39.0	36.0	40.0	32.0	41.0
20	37.41889374906391	39.0	36.0	40.0	32.0	41.0
21	37.38066307466023	39.0	36.0	40.0	32.0	41.0
22	37.3122030930171	39.0	36.0	40.0	32.0	41.0
23	37.138381216084916	39.0	35.0	40.0	32.0	41.0
24	37.040732661784304	39.0	35.0	40.0	31.0	41.0
25	37.03583045627187	39.0	35.0	40.0	31.0	41.0
26	36.959405590780094	39.0	35.0	40.0	31.0	41.0
27	36.87022116569954	39.0	35.0	40.0	31.0	41.0
28	36.75874927513412	39.0	35.0	40.0	31.0	41.0
29	36.554517594558995	39.0	35.0	40.0	30.0	41.0
30	36.57706812395111	39.0	35.0	40.0	31.0	41.0
31	36.22611590941385	38.0	35.0	40.0	30.0	41.0
32	36.26734013587155	38.0	35.0	40.0	30.0	41.0
33	36.26466149243643	38.0	35.0	40.0	30.0	41.0
34	36.24533109568998	38.0	35.0	40.0	30.0	41.0
35	36.21672049555863	38.0	35.0	40.0	30.0	41.0
36	36.09434009362771	38.0	35.0	40.0	30.0	41.0
37	36.03037908085087	38.0	35.0	40.0	30.0	41.0
38	35.920662229783446	38.0	35.0	40.0	29.0	41.0
39	35.81760646407545	38.0	35.0	40.0	29.0	41.0
40	35.65865057816455	38.0	35.0	40.0	28.0	41.0
41	35.64192393804749	38.0	35.0	40.0	28.0	41.0
42	35.52914440864386	38.0	34.0	40.0	27.0	41.0
43	35.45811523351242	38.0	34.0	40.0	27.0	41.0
44	35.23325703839965	38.0	34.0	40.0	26.0	41.0
45	34.98168345539243	38.0	34.0	40.0	26.0	41.0
46	34.64372314155911	37.0	33.0	40.0	24.0	41.0
47	34.59917317285795	37.0	33.0	40.0	24.0	41.0
48	34.55183127042586	37.0	33.0	40.0	24.0	41.0
49	34.47320588495082	37.0	33.0	40.0	24.0	41.0
50	34.29211038699197	37.0	33.0	40.0	24.0	41.0
51	33.14461218235513	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	4.0
12	6.0
13	6.0
14	10.0
15	22.0
16	65.0
17	132.0
18	254.0
19	545.0
20	906.0
21	1385.0
22	1928.0
23	2696.0
24	3299.0
25	3854.0
26	4690.0
27	5752.0
28	7398.0
29	9310.0
30	11781.0
31	15672.0
32	20304.0
33	26627.0
34	37651.0
35	53992.0
36	57711.0
37	73742.0
38	94724.0
39	86301.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.819054275652572	34.2163575825771	22.52883142019947	24.435756721570854
2	23.080113520716765	32.92964864646899	20.007642294531728	23.98259553828252
3	19.34345393309344	36.01536907674169	19.715391734800857	24.92578525536401
4	19.391458295729915	36.73370635923393	19.205009351249842	24.669825993786315
5	17.085136697223042	39.9824496050201	16.08453376242833	26.84787993532852
6	16.049010534077336	45.999124400425515	16.76638772931684	21.185477336180313
7	82.10032527756123	10.633734393781708	3.804249730215482	3.4616905984415864
8	83.82060961700199	9.730484306413768	2.81055942364042	3.6383466529438198
9	77.42412430441678	13.699869044098728	5.711559066487962	3.164447584996524
10	28.192386124050955	50.6073511960767	9.484317934813916	11.715944745058431
11	20.16317642947391	32.83114369433894	30.11640097852093	16.88927889766622
12	22.200289562315422	30.27001493895765	30.052843202390235	17.47685229633669
13	23.43534580422669	31.02233931019651	27.2100248470581	18.3322900385187
14	18.349379591617286	35.34177186022666	27.988847626472296	18.320000921683764
15	17.277538182670042	35.24595515240425	28.57526892043949	18.901237744486217
16	18.836335846201703	35.68529107925328	26.56331007361949	18.915063000925525
17	18.50971416282312	33.93543605242845	27.452734904548127	20.10211488020031
18	20.359034229030733	32.863978678382296	27.456767271009593	19.320219821577385
19	19.06310845529642	36.60524668481872	26.818885300296092	17.512759559588776
20	20.52301713179694	35.69892431824204	26.049087341057554	17.728971208903467
21	20.525129323752942	35.432404096884326	26.297941956965047	17.744524622397684
22	19.872462009347412	33.12819469033346	25.546961707880012	21.45238159243912
23	18.20479045135622	36.72429750415718	24.91388017343016	20.15703187105644
24	16.792502102591083	36.27785693163795	27.189286962399144	19.740354003371827
25	18.06404166010607	37.58434366515229	24.249691811991873	20.101922862749767
26	21.064698359786938	36.06567764878472	24.043465070105572	18.82615892132277
27	18.36051660374895	34.95869704638758	25.616472024977632	21.064314324885846
28	17.82248370731932	36.27401658262703	26.142791856923957	19.76070785312969
29	18.688674426731904	36.67821331602616	24.16366799414731	20.46944426309463
30	20.295668470350588	35.88268501841447	24.69766852411547	19.12397798711947
31	21.912455403947114	35.1591632647575	23.052847042739245	19.875534288556143
32	19.696382007196814	35.07429155161621	23.961665636173016	21.26766080501396
33	18.89317301156329	35.06065831262745	24.925017185561824	21.121151490247435
34	17.64064318165235	35.776499368262584	25.649114991570432	20.93374245851463
35	19.25013345212813	34.72097944261174	26.10899678562788	19.91989031963225
36	20.424320162216343	36.40382037919606	25.166959173249666	18.004900285337932
37	19.127050266328204	35.60233954061745	24.26006075432135	21.01054943873299
38	19.93198741901664	33.98824085132857	26.072513470024155	20.007258259630635
39	20.15280748714443	32.57211215355251	24.074763914544555	23.2003164447585
40	21.641134746325747	34.009362770888615	23.06936054348619	21.280141939299444
41	18.682721885764977	33.875334590407576	25.54580960317674	21.89613392065071
42	21.25498765327793	33.578091576962514	24.035400337182644	21.131520432576913
43	20.115556101738527	33.32136424558264	25.71593706436041	20.847142588318427
44	21.20583118593818	32.59265802076093	24.623741805655296	21.577768987645598
45	21.342547610726864	32.350331998171995	26.146440188484327	20.160680202616813
46	19.635896510274854	33.372824922328945	26.562157968916217	20.42912059847999
47	19.9807598514553	35.316041521853506	24.473200124427308	20.229998502263886
48	19.9339075935221	33.00741571394008	27.360182493385	19.69849419915282
49	20.48154136247902	32.15159393685698	25.237237560149467	22.12962714051453
50	18.719013183918154	32.127399738088194	25.63624982238386	23.517337255609792
51	19.97327117088401	29.73275011233021	26.578479452212616	23.715499264573165
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17428.0
1	13040.0
2	8652.0
3	5403.0
4	2154.0
5	1724.5
6	1295.0
7	1469.5
8	1644.0
9	1881.5
10	2119.0
11	2316.5
12	2514.0
13	2829.0
14	3144.0
15	3298.5
16	3453.0
17	3348.5
18	3244.0
19	3301.5
20	3359.0
21	3366.0
22	3373.0
23	4054.5
24	4736.0
25	4982.0
26	5862.0
27	6496.0
28	7302.0
29	8108.0
30	9840.5
31	11573.0
32	14038.5
33	16504.0
34	17080.5
35	17657.0
36	18894.5
37	20132.0
38	20970.0
39	21808.0
40	22110.5
41	22413.0
42	25666.5
43	28920.0
44	32499.5
45	36079.0
46	38972.5
47	41866.0
48	45529.5
49	49193.0
50	47724.5
51	46256.0
52	40973.5
53	35691.0
54	31118.0
55	26545.0
56	22337.5
57	18130.0
58	16232.5
59	14335.0
60	13034.5
61	11734.0
62	9901.0
63	8068.0
64	7319.0
65	6570.0
66	5246.0
67	3922.0
68	3171.5
69	2421.0
70	1967.0
71	1513.0
72	1350.5
73	1188.0
74	879.0
75	414.0
76	258.0
77	192.5
78	127.0
79	113.5
80	100.0
81	130.5
82	161.0
83	107.0
84	53.0
85	43.0
86	33.0
87	19.5
88	6.0
89	5.5
90	5.0
91	5.5
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	520786.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.419866522222776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.80048501774225	22.300401515417242
2	9.820603010285994	5.778416594607063
3	3.8139915202331647	3.366213643264477
4	2.095262179121914	2.4656933455531336
5	1.3020970941998262	1.9153761355166512
6	0.9189754619529193	1.622168125671174
7	0.701585492991105	1.444838609040832
8	0.5505991978504612	1.2958843926402799
9	0.42477237715764893	1.1247071974474772
>10	3.532253597213918	22.069495748776717
>50	0.6728863895962991	13.980915872885872
>100	0.35988526234542	16.96258911882218
>500	0.003301699654545138	0.6949078186548198
>1k	0.0026413597236361105	1.9817597590432814
>5k	0.0	0.0
>10k+	6.603399309090276E-4	2.996632122658819
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15425	2.961869174670594	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3682	0.7070082529100244	No Hit
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	2468	0.473899067947295	TruSeq Adapter, Index 14 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2428	0.46621836992545884	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1623	0.3116443222360048	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGT	845	0.16225474571129025	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCC	757	0.14535721006325056	No Hit
AAGCGAGGGAAGGAAATTGAAAAAGGAACCTAATTAATTCTTACAAATTGC	732	0.1405567737996029	No Hit
AATCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCT	652	0.12519537775593045	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	591	0.11348231327263023	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5931419047363025	0.0
2	0.0	0.0	0.0	0.7538605108432255	0.0
3	0.0	0.0	0.0	0.9512544500044164	0.0
4	0.0	0.0	0.0	1.9172942437008675	0.0
5	0.0	0.0	0.0	2.068603994731041	0.0
6	0.0	0.0	0.0	2.9442035692203707	0.0
7	0.0	0.0	0.0	3.6452592811634723	0.0
8	0.0	0.0	0.0	4.374349540886276	0.0
9	0.0	0.0	0.0	5.4799860211296005	0.0
10	0.0	0.0	0.0	7.037439562507441	0.0
11	0.0	0.0	0.0	8.506565076634164	0.0
12	0.0	0.0	0.0	9.258505412971932	0.0
13	0.0	0.0	0.0	9.581094729889053	0.0
14	0.0	0.0	0.0	9.920197547553121	0.0
15	0.0	0.0	0.0	10.204767409262153	0.0
16	0.0	0.0	0.0	10.783892040108604	0.0
17	0.0	0.0	0.0	11.622432246642575	0.0
18	0.0	0.0	0.0	12.739205739017562	0.0
19	0.0	0.0	0.0	13.168556758438207	0.0
20	0.0	0.0	0.0	13.60501242352905	0.0
21	0.0	0.0	0.0	14.055101327608654	0.0
22	0.0	0.0	0.0	14.633841923554012	0.0
23	0.0	0.0	0.0	15.187620250928404	0.0
24	0.0	0.0	0.0	15.573191291624584	0.0
25	0.0	0.0	0.0	15.907301655574459	0.0
26	0.0	0.0	0.0	16.259269642425103	0.0
27	0.0	0.0	0.0	16.60451701850664	0.0
28	0.0	0.0	0.0	16.937667295203788	0.0
29	0.0	0.0	0.0	17.303076503592646	0.0
30	0.0	0.0	0.0	17.782544077605774	0.0
31	0.0	0.0	0.0	18.13720030876406	0.0
32	0.0	0.0	0.0	18.514130564185674	0.0
33	0.0	0.0	0.0	18.88760450549746	0.0
34	0.0	0.0	0.0	19.261654499160883	0.0
35	0.0	0.0	0.0	19.676220174889494	0.0
36	0.0	0.0	0.0	20.022811673124853	0.0
37	0.0	0.0	0.0	20.394557457381726	0.0
38	0.0	0.0	0.0	20.756510351660758	0.0
39	0.0	0.0	0.0	21.137088938642744	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGT	20	7.02973E-4	45.0	19
CGGACAG	25	3.887857E-5	45.0	1
TCACGAG	20	7.02973E-4	45.0	1
CCTTCCG	20	7.02973E-4	45.0	19
TTAGGTC	20	7.02973E-4	45.0	19
CGGCGGA	20	7.02973E-4	45.0	18
AATCCGA	20	7.02973E-4	45.0	17
TCGTTCA	25	3.887857E-5	45.0	16
TCGCACA	20	7.02973E-4	45.0	15
CGAACAG	45	3.8380676E-10	45.0	1
GGTGTTA	20	7.02973E-4	45.0	9
GCGACGC	45	3.8380676E-10	45.0	15
TCCGCGG	20	7.02973E-4	45.0	2
GTCTGCG	35	1.21021E-7	45.0	1
CAACGAA	20	7.02973E-4	45.0	32
CATGCGG	20	7.02973E-4	45.0	2
CGACGAA	20	7.02973E-4	45.0	17
TAGACGG	70	0.0	45.0	2
TAGCGAG	35	1.21021E-7	45.0	1
GTCTCGC	35	1.21021E-7	45.0	25
>>END_MODULE